Standard name
Human Ortholog
Description Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 – 0.99 0.99 0.86 0.97 0.92 0.82 0.85 0.87 0.84 0.83 0.98 0.94 0.93 0.98 0.96 0.97 0.95 0.86 0.94 0.91 0.93 0.77
Endoplasmic Reticulum 0 – 0 0 0 0 0.06 0.09 0.07 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0.14 0 0 0.07 0 0 0 0 0 0.13 0.15 0 0 0 0 0.05 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 1 2 7 6 6 8 0 0 0 0 0 0 0 0 0 0 0 3
Bud 2 – 0 1 0 0 2 2 2 0 1 2 0 0 0 0 2 0 1 0 0 0 0 2
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 – 2 0 3 1 3 8 7 10 4 6 2 0 0 4 0 0 0 0 0 0 0 0
Cytoplasm 249 – 170 149 197 218 287 375 225 256 191 239 173 289 272 169 157 107 234 42 154 81 206 243
Endoplasmic Reticulum 3 – 1 0 4 3 18 43 18 15 10 13 1 3 3 5 10 3 0 0 2 2 1 12
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 2 0 0 1 2 1 5 1 1 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 – 0 0 0 0 1 5 7 13 9 19 1 1 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 1 2 2 1 0 2 4 5 1 6 0 1 1 0 3 2 4 2 1 1 5 17
Nuclear Periphery 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 2
Nucleolus 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 – 2 0 32 4 7 34 2 6 7 7 7 39 43 5 1 3 2 2 2 0 1 22
Unique Cell Count 254 172 150 230 224 312 457 264 295 227 289 177 308 292 173 164 110 248 49 165 89 222 315
Labelled Cell Count 261 176 152 240 227 319 473 274 313 234 301 185 333 319 183 174 115 248 49 165 89 222 315


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.9 – 29.1 29.4 32.1 25.8 26.5 23.8 22.7 21.4 20.8 21.0 34.7 33.6 32.3 39.1 44.4 53.4 29.9 29.6 31.6
Std Deviation (1e-4) 6.1 – 6.4 5.9 13.0 5.8 6.4 5.7 4.6 5.1 5.0 5.4 8.3 7.0 7.4 11.9 15.2 14.4 8.8 7.0 8.5
Intensity Change (Log2) – – – 0.02 0.14 -0.18 -0.14 -0.29 -0.36 -0.44 -0.49 -0.47 0.25 0.21 0.15 0.43 0.61 0.88 0.04 0.02 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -4.6 -1.1 -3.1 -5.5 -4.8 -4.4 -5.0 -5.3 -0.8 -2.6 -2.8 -0.8 -1.8 -1.0
Endoplasmic Reticulum 0 0 0 2.8 3.9 3.1 2.6 0 2.4 0 0 0 0 2.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.5 0 0 3.0 0 0 0 0 0 4.3 4.8 0 0 0