Standard name
Human Ortholog
Description Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0.07 0.06 0.06 0.05 0 0.05 0.05 0 0 0.05 0.1 0.15 0.07 0.15 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.25 0 0.1 0 0 0.15 0.3 0.19 0.41 0.36 0.43 0.27 0 0 0 0 0.05 – 0 0 0 0 0 0
Nucleus 0.9 0.85 0.93 0.91 0.91 0.86 0.89 0.86 0.82 0.83 0.79 0.87 0.92 0.87 0.83 0.79 0.85 – 0.85 0.79 0.79 0.84 0.74 0.75
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.2 0.11 0.08 0.06 0 0.05 0.09 0.14 0.14 0.14 0.2 0.14 0 0 0 0.14 0.15 – 0.09 0.05 0.08 0.06 0.13 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0.09 0.05 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 1 2 0 2 1 1 1 0 0 0 0 0 – 2 0 0 0 2 3
Bud Neck 0 0 0 1 0 2 1 0 0 2 0 1 1 0 0 0 0 – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 5 6 8 13 9 13 6 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 3 6 1 11 14 12 12 9 12 11 8 3 12 39 50 4 3 – 0 2 0 4 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 0 0 2 2
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 2 1 0 2 0 – 0 0 0 2 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Mitochondria 50 2 9 4 7 32 80 44 101 87 97 64 7 3 1 1 1 – 1 0 1 1 1 2
Nucleus 183 63 81 153 205 182 234 197 203 200 180 206 208 328 275 45 17 – 172 68 82 170 257 245
Nuclear Periphery 1 0 0 1 2 3 2 1 1 1 0 1 1 1 1 0 0 – 0 0 1 0 2 0
Nucleolus 40 8 7 10 2 11 25 33 35 33 46 32 4 8 5 8 3 – 17 4 8 13 46 31
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 1
SpindlePole 0 0 1 5 4 3 2 3 2 2 1 2 10 6 9 0 0 – 0 0 2 1 7 6
Vac/Vac Membrane 1 1 1 0 1 1 1 0 0 0 0 0 0 2 2 3 0 – 4 7 4 6 19 18
Unique Cell Count 203 74 87 168 226 211 264 228 248 240 227 236 226 377 333 57 20 203 87 105 203 349 327
Labelled Cell Count 278 80 100 185 235 252 365 296 369 346 346 316 245 390 343 63 24 203 87 105 203 349 327


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 6.9 5.3 5.9 6.3 5.2 4.7 5.0 4.4 4.4 4.3 4.6 6.0 6.9 6.8 9.2 8.7 – 6.7 6.4 7.2
Std Deviation (1e-4) 0.9 1.2 0.8 1.8 2.0 1.6 1.2 1.2 1.2 1.3 1.3 1.0 1.2 1.6 1.6 1.7 1.6 – 1.8 1.7 1.9
Intensity Change (Log2) – – – 0.17 0.25 -0.03 -0.18 -0.09 -0.28 -0.27 -0.3 -0.2 0.19 0.37 0.36 0.79 0.71 – 0.33 0.28 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.8 3.5 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 1.1 0 1.9 0 0 0 0 0 0 0 0 0 –
Nucleus -0.6 -0.7 -1.7 -1.2 -1.7 -2.5 -2.2 -2.9 -1.5 -0.3 -1.6 -2.4 -2.5 -1.2 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus -0.6 0 -0.9 0 1.5 0 0 0 0 0 0 0 1.2 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5846 1.2703 1.2201 1.087 0.5877 1.2111 2.6273 1.829 1.6471 1.5431 1.4416 1.8168 1.7584 1.007 1.0568 0.9034 1.4148 1.2927
Actin 0.0073 0 0.0014 0.0215 0.0002 0.0017 0.0089 0 0.0025 0.0002 0.001 0.0003 0.0368 0 0.0191 0 0.0118 0.002
Bud 0.0002 0.0001 0.0001 0.0001 0 0 0.0008 0 0.0003 0.0002 0.0004 0.0001 0.0011 0 0.0003 0 0.0007 0.0002
Bud Neck 0.0009 0.0001 0.0003 0.0002 0.0003 0.0006 0.0021 0.0001 0.001 0.0007 0.0011 0.0009 0.0017 0.0001 0.0006 0 0.0013 0.0011
Bud Periphery 0.0003 0 0.0002 0.0002 0 0.0001 0.0021 0 0.0015 0.0004 0.0009 0.0003 0.0051 0.0001 0.0005 0 0.0019 0.0005
Bud Site 0.0006 0.0001 0.0002 0.0002 0.0001 0.0001 0.0037 0 0.0038 0.0043 0.0023 0.0002 0.0015 0.0001 0.0014 0 0.0025 0.0005
Cell Periphery 0.0001 0 0 0 0 0 0.0006 0 0.0006 0.0006 0.0003 0 0.0007 0 0.0001 0 0.0003 0.0001
Cytoplasm 0.0049 0.0003 0.0006 0.0007 0.0001 0.0002 0.0062 0 0.0025 0.0011 0.0075 0.0003 0.0057 0.0067 0.0003 0 0.0017 0.0006
Cytoplasmic Foci 0.0059 0 0.001 0.0021 0.002 0.0012 0.0101 0 0.0062 0.0005 0.0032 0.0019 0.0206 0 0.0051 0 0.039 0.0014
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0003 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0039 0 0.0007 0.0018 0 0.0016 0.0051 0 0.0003 0.0004 0.0031 0.0002 0.0078 0 0.0006 0 0.0175 0.0013
Endosome 0.015 0 0.0038 0.0042 0.0004 0.0033 0.0315 0 0.004 0.0062 0.0318 0.0024 0.0149 0.0001 0.0091 0 0.0938 0.012
Golgi 0.0018 0 0.0004 0.0032 0.0006 0.0012 0.0063 0 0.0009 0.0006 0.0024 0.0003 0.0081 0 0.0078 0 0.0601 0.0028
Lipid Particles 0.0105 0 0.0004 0.0028 0.0202 0.0018 0.0183 0 0.0096 0.0004 0.0235 0.0016 0.0191 0 0.0045 0 0.0401 0.0039
Mitochondria 0.0016 0.0001 0.0011 0.002 0.0039 0.0014 0.0162 0 0.006 0.0005 0.0132 0.0006 0.0137 0.0002 0.0072 0 0.0531 0.0102
None 0.0116 0.0001 0.0093 0.0005 0.0001 0.0002 0.0033 0 0.001 0.0001 0.0016 0.0003 0.0072 0.0002 0.0001 0 0.0025 0.0003
Nuclear Periphery 0.035 0.0008 0.0029 0.024 0.0013 0.0076 0.0257 0.001 0.0026 0.0007 0.0255 0.0021 0.0229 0.0015 0.0013 0.0003 0.1045 0.0094
Nucleolus 0.0335 0.0286 0.0221 0.0138 0.0706 0.0568 0.0478 0.021 0.0232 0.02 0.1033 0.0708 0.0287 0.0323 0.0179 0.0145 0.0438 0.057
Nucleus 0.8505 0.9626 0.9359 0.9146 0.8462 0.9115 0.7829 0.9758 0.9223 0.9492 0.7501 0.9074 0.759 0.9573 0.9175 0.9849 0.4721 0.8827
Peroxisomes 0.0009 0 0.0001 0.0035 0.0127 0.0005 0.0051 0 0.0014 0.0001 0.0014 0.0008 0.0142 0 0.0039 0 0.0253 0.0026
Punctate Nuclear 0.0117 0.0072 0.0184 0.0032 0.0412 0.0092 0.0118 0.0019 0.007 0.0006 0.0138 0.0076 0.0183 0.0011 0.0004 0.0002 0.0096 0.0087
Vacuole 0.0019 0 0.0005 0.0003 0.0001 0.0003 0.0081 0 0.0024 0.0118 0.008 0.0015 0.0069 0.0002 0.0014 0 0.0065 0.0013
Vacuole Periphery 0.0016 0 0.0005 0.0008 0 0.0005 0.0031 0 0.0009 0.0012 0.0055 0.0004 0.0057 0.0001 0.0009 0 0.0115 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8968 9.7276 12.9929 11.4871 16.2249 9.7881 10.0814 12.2809 10.5687 12.554
Translational Efficiency 2.6113 1.0259 0.8701 1.0489 0.7172 1.0351 1.2271 1.0779 1.2241 0.9407

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1524 246 1943 1474 1900 1335 298 990 3424 1581 2241 2464

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.14 873.55 963.60 840.90 769.57 827.37 1069.79 980.26 792.97 834.56 977.72 896.89
Standard Deviation 113.09 127.65 125.97 167.01 118.74 132.87 120.74 154.02 119.16 133.12 130.38 175.74
Intensity Change Log 2 — 0.087506 0.229050 0.032550 — 0.104480 0.475203 0.349112 — 0.095738 0.353309 0.194280

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000454 0.000862 0.000658 0.001217 0.000394 0.000664 0.001514 0.001073 0.000420 0.000695 0.000771 0.001159
Bud Neck 0.005273 0.014906 0.025621 0.016923 0.007989 0.013157 0.012489 0.030892 0.006780 0.013429 0.023875 0.022535
Bud Site 0.000936 0.001623 0.001375 0.004130 0.001700 0.001892 0.001378 0.003739 0.001360 0.001850 0.001376 0.003973
Cell Periphery 0.000056 0.000079 0.000035 0.000169 0.000097 0.000128 0.000042 0.000091 0.000079 0.000120 0.000036 0.000137
Cytoplasm 0.001690 0.003431 0.000242 0.008262 0.002291 0.003018 0.000183 0.002386 0.002023 0.003083 0.000234 0.005901
Cytoplasmic Foci 0.000407 0.001018 0.000007 0.000387 0.000193 0.000830 0.000039 0.000774 0.000288 0.000859 0.000011 0.000542
Eisosomes 0.000023 0.000031 0.000035 0.000026 0.000022 0.000029 0.000058 0.000031 0.000022 0.000030 0.000038 0.000028
Endoplasmic Reticulum 0.002433 0.003670 0.002797 0.005575 0.002941 0.003416 0.005617 0.005071 0.002715 0.003456 0.003172 0.005373
Endosome 0.000195 0.000480 0.000093 0.000757 0.000340 0.000656 0.000167 0.002945 0.000275 0.000629 0.000103 0.001636
Golgi 0.000113 0.000251 0.000085 0.001468 0.000166 0.000383 0.000139 0.001468 0.000143 0.000362 0.000092 0.001468
Lipid Particles 0.000193 0.000283 0.000031 0.000156 0.000128 0.000255 0.000131 0.000224 0.000157 0.000259 0.000044 0.000183
Mitochondria 0.002016 0.001578 0.001218 0.002833 0.001481 0.003144 0.001180 0.004006 0.001720 0.002900 0.001213 0.003304
Mitotic Spindle 0.005642 0.001786 0.000943 0.005232 0.001880 0.005993 0.000517 0.012346 0.003555 0.005338 0.000886 0.008090
None 0.003184 0.004372 0.000991 0.008690 0.005735 0.004283 0.001652 0.004409 0.004600 0.004297 0.001078 0.006970
Nuclear Periphery 0.000340 0.000300 0.000214 0.000750 0.000382 0.000600 0.000207 0.000962 0.000363 0.000553 0.000213 0.000835
Nuclear Periphery Foci 0.000265 0.000207 0.000036 0.000649 0.000359 0.000327 0.000163 0.000379 0.000317 0.000308 0.000053 0.000540
Nucleolus 0.038492 0.044531 0.032775 0.012212 0.040719 0.041951 0.040563 0.013123 0.039728 0.042352 0.033811 0.012578
Nucleus 0.937102* 0.916831* 0.932021* 0.926823* 0.932161* 0.916438* 0.931123* 0.910603* 0.934360* 0.916499* 0.931901* 0.920306*
Peroxisomes 0.000714 0.002400 0.000266 0.000262 0.000311 0.000958 0.001136 0.000323 0.000490 0.001182 0.000381 0.000286
Vacuole 0.000325 0.001059 0.000417 0.002752 0.000513 0.001078 0.001518 0.003787 0.000429 0.001075 0.000563 0.003168
Vacuole Periphery 0.000148 0.000301 0.000142 0.000729 0.000199 0.000801 0.000184 0.001368 0.000176 0.000723 0.000147 0.000986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.77 -2.60 -5.75 -2.98 -5.66 -1.72 -12.00 -6.82 -3.30 1.04 -1.94 -7.68 -9.64 -3.53 -5.52
Bud Neck -3.71 -19.62 -11.88 -2.15 3.87 -4.70 -5.17 -11.64 -9.06 -8.16 -6.88 -19.26 -16.04 -9.09 -0.95
Bud Site -1.23 -1.92 -3.46 -2.47 -2.70 -0.37 -0.15 -3.82 -4.00 -4.93 -1.15 -0.84 -4.59 -3.73 -4.49
Cell Periphery -1.16 1.70 -4.98 -3.06 -6.96 -0.89 3.01 0.43 1.27 -5.42 -1.56 3.84 -3.34 -0.31 -8.47
Cytoplasm -0.79 3.18 -4.91 -1.90 -6.42 -1.04 4.45 -1.52 -0.28 -4.87 -1.53 5.43 -4.99 -2.73 -7.71
Cytoplasmic Foci -0.77 1.75 0.40 0.92 -3.00 -1.81 3.31 -1.06 0.97 -1.86 -1.75 2.75 -0.47 1.54 -3.26
Eisosomes -2.14 -7.25 -2.34 1.06 6.91 -3.39 -11.58 -9.08 -3.78 6.38 -3.77 -13.30 -7.67 -1.10 6.14
Endoplasmic Reticulum -3.48 -2.03 -10.31 -4.92 -8.82 -1.72 -5.05 -8.66 -7.76 -0.39 -3.42 -2.80 -12.93 -9.36 -9.74
Endosome -1.49 2.49 -2.76 -0.72 -3.39 -2.35 2.20 -1.89 -1.48 -2.10 -3.23 4.17 -2.56 -1.44 -3.09
Golgi -1.11 0.56 -1.81 -1.52 -1.83 -1.97 -0.14 -1.67 -1.14 -1.66 -2.47 1.22 -2.34 -1.72 -2.43
Lipid Particles -0.55 5.11 1.09 0.82 -3.71 -2.23 -0.38 -2.51 -0.31 -2.21 -1.93 5.92 -1.13 1.14 -5.38
Mitochondria 0.53 0.94 -0.98 -1.92 -2.69 -1.93 0.16 -2.92 -0.77 -3.87 -1.67 0.82 -2.64 -0.48 -4.44
Mitotic Spindle 1.89 2.37 -0.27 -2.39 -3.07 -2.16 0.57 -4.38 -3.05 -4.55 -0.96 2.37 -3.74 -2.56 -5.51
None -0.96 4.15 -3.49 -1.83 -5.82 1.38 4.74 1.64 0.26 -3.63 0.39 6.70 -1.64 -1.88 -7.03
Nuclear Periphery 0.59 1.22 -5.33 -6.04 -7.02 -1.68 1.09 -7.15 -4.88 -8.30 -1.77 1.95 -8.64 -5.01 -10.79
Nuclear Periphery Foci 0.94 3.34 -1.83 -2.20 -2.89 0.85 1.61 -1.02 -1.62 -2.09 0.62 4.55 -1.93 -2.27 -3.92
Nucleolus -1.20 0.69 12.93 6.50 12.10 -0.33 -1.24 10.51 9.40 5.80 -1.05 0.80 17.11 12.96 12.85
Nucleus 2.31 3.26 2.93 -0.70 0.36 3.07 2.00 6.08 3.31 3.29 4.19 3.40 6.04 1.08 3.37
Peroxisomes -0.93 1.42 1.55 1.17 1.54 -2.88 -7.27 -1.64 2.45 6.21 -1.98 0.55 1.28 2.56 3.01
Vacuole -1.51 -1.96 -4.35 -2.19 -3.95 -2.12 -3.30 -5.95 -5.13 -4.06 -2.81 -2.52 -7.25 -5.35 -6.63
Vacuole Periphery -1.34 -0.08 -2.59 -1.49 -2.60 -2.67 0.10 -2.53 -0.17 -2.55 -2.88 0.93 -3.56 0.05 -3.73
Standard name
Human Ortholog
Description Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt3

Spt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available