Standard name | |
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Human Ortholog | |
Description | Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 |
Bud Neck | 2 | 2 | 1 | 3 | 0 | 3 | 5 | – | 1 | 5 | 5 | 6 | 5 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 1 | 0 | 1 | 1 | 1 | 3 | – | 9 | 6 | 2 | 8 | 1 | 1 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 5 | 5 | 4 | 10 | 8 | 11 | – | 1 | 6 | 5 | 1 | 14 | 26 | 30 | 10 | 10 | 10 | 0 | 3 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 2 | 4 | 8 | 8 | 17 | – | 8 | 14 | 2 | 8 | 3 | 4 | 9 | 1 | 4 | 1 | 0 | 3 | 2 | 2 | 4 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 36 | 1 | 6 | 5 | 15 | 15 | – | 19 | 14 | 28 | 11 | 1 | 4 | 2 | 30 | 20 | 26 | 0 | 1 | 1 | 0 | 0 | 2 |
Nucleus | 21 | 57 | 33 | 16 | 17 | 5 | 16 | – | 9 | 20 | 17 | 10 | 13 | 8 | 8 | 31 | 27 | 31 | 8 | 45 | 22 | 15 | 27 | 19 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 90 | 364 | 139 | 136 | 201 | 181 | 244 | – | 173 | 210 | 170 | 171 | 169 | 197 | 185 | 222 | 199 | 176 | 80 | 338 | 129 | 145 | 150 | 173 |
Peroxisomes | 0 | 4 | 1 | 0 | 1 | 6 | 5 | – | 2 | 1 | 3 | 4 | 0 | 1 | 5 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 6 | 1 | 23 | 12 | 27 | 42 | 58 | – | 61 | 75 | 35 | 65 | 18 | 20 | 26 | 3 | 2 | 6 | 2 | 12 | 7 | 4 | 13 | 12 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 3 | 6 | 5 | 0 | 4 | 1 | 1 | 4 | 6 |
Unique Cell Count | 94 | 398 | 164 | 152 | 226 | 200 | 276 | 199 | 254 | 193 | 187 | 198 | 234 | 234 | 256 | 236 | 213 | 92 | 418 | 172 | 175 | 209 | 232 | |
Labelled Cell Count | 126 | 475 | 206 | 182 | 273 | 269 | 375 | 286 | 354 | 272 | 284 | 225 | 265 | 270 | 304 | 276 | 258 | 92 | 418 | 172 | 175 | 209 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.5 | 23.0 | 17.8 | 16.3 | 15.0 | 12.0 | 11.2 | – | 9.5 | 10.3 | 9.1 | 9.4 | 19.0 | 17.5 | 16.4 | 27.7 | 28.2 | 26.0 | 16.6 | 16.6 | 16.7 |
Std Deviation (1e-4) | 5.2 | 6.8 | 6.0 | 4.8 | 4.9 | 4.1 | 2.7 | – | 3.2 | 3.5 | 2.9 | 3.1 | 8.4 | 6.8 | 7.3 | 12.9 | 11.5 | 11.8 | 5.0 | 5.4 | 5.4 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.24 | -0.57 | -0.67 | – | -0.91 | -0.78 | -0.97 | -0.92 | 0.09 | -0.03 | -0.11 | 0.64 | 0.67 | 0.55 | -0.09 | -0.1 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.7 | 0.5 | 0.5 | – | 0 | 0 | 0 | 0 | 1.7 | 3.0 | 3.4 | 0.5 | 0.6 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 2.5 | – | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.2 | 2.6 | – | 3.7 | 2.6 | 4.8 | 2.7 | 0 | 0 | 0 | 4.2 | 3.5 | 4.3 |
Nucleus | -2.4 | -3.7 | -5.5 | -4.6 | – | -4.6 | -3.7 | -3.1 | -4.2 | -3.9 | -5.4 | -5.4 | -2.2 | -2.4 | -1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | 1.2 | 1.7 | 1.1 | – | 0.6 | -0.6 | 0.9 | 1.9 | 0.2 | -0.2 | -1.4 | 0.6 | -0.1 | -0.6 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.7 | -0.6 | 1.7 | 1.8 | – | 3.7 | 3.7 | 1.0 | 4.5 | -1.5 | -1.7 | -0.9 | -5.3 | -5.4 | -4.0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.278 | 13.1794 | 12.6863 | 11.6057 | 12.8058 | 11.3404 | 12.2559 | 15.0037 | 13.0881 | 11.8826 | 10.3793 | 12.9066 | 14.4916 | 15.3324 | 14.2936 | 12.9077 | 12.3184 | 13.082 |
Actin | 0.0104 | 0 | 0 | 0.0058 | 0 | 0.0004 | 0.0019 | 0 | 0.0006 | 0 | 0.0017 | 0.001 | 0.0147 | 0.0001 | 0.011 | 0.0095 | 0.0049 | 0 |
Bud | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.0001 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0027 | 0.0006 | 0.0001 |
Bud Periphery | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0 |
Bud Site | 0.0015 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0007 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0034 | 0.0002 | 0 |
Cell Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0001 |
Cytoplasm | 0.0036 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0011 | 0 | 0.001 | 0.0002 | 0.0026 | 0 | 0.0006 | 0 | 0 | 0.0012 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0036 | 0.0001 | 0.0006 | 0.0023 | 0.0004 | 0.0082 | 0.0086 | 0 | 0.0077 | 0.0016 | 0.0239 | 0.0003 | 0.0068 | 0 | 0.001 | 0.0245 | 0.0004 | 0.0001 |
Eisosomes | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0081 | 0 | 0 | 0.0003 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0.0001 | 0.0041 | 0.0004 | 0.0007 | 0 | 0.0001 | 0.0008 | 0.0022 | 0 |
Endosome | 0.0111 | 0 | 0 | 0.0045 | 0 | 0.0008 | 0.0101 | 0 | 0.0042 | 0.0004 | 0.0298 | 0.0018 | 0.0041 | 0 | 0.0001 | 0.0042 | 0.0082 | 0 |
Golgi | 0.0028 | 0 | 0 | 0.006 | 0 | 0.0011 | 0.0013 | 0 | 0.0005 | 0.0001 | 0.0076 | 0.0006 | 0.0019 | 0 | 0.0003 | 0.0025 | 0.0014 | 0 |
Lipid Particles | 0.0105 | 0.0002 | 0.0005 | 0.0564 | 0.0017 | 0.0041 | 0.0185 | 0.0001 | 0.0046 | 0.0019 | 0.0294 | 0.0006 | 0.0044 | 0.0001 | 0.0005 | 0.0119 | 0.0018 | 0.0002 |
Mitochondria | 0.0152 | 0.0001 | 0.0016 | 0.0266 | 0.0417 | 0.0318 | 0.0034 | 0.0001 | 0.001 | 0.0003 | 0.0341 | 0.0032 | 0.0007 | 0.001 | 0.0005 | 0.0068 | 0.0061 | 0.0001 |
None | 0.0008 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0.0013 | 0 | 0.0006 | 0.0001 | 0.0014 | 0.0001 | 0.002 | 0 | 0 | 0.0037 | 0.0012 | 0 |
Nuclear Periphery | 0.0015 | 0 | 0 | 0.0043 | 0 | 0 | 0.0191 | 0 | 0.0033 | 0.0019 | 0.0078 | 0.0052 | 0.0013 | 0.0003 | 0.0001 | 0.0037 | 0.0399 | 0 |
Nucleolus | 0.8681 | 0.9287 | 0.9296 | 0.8081 | 0.8765 | 0.9021 | 0.8256 | 0.8877 | 0.8756 | 0.866 | 0.7715 | 0.9196 | 0.8699 | 0.8781 | 0.9061 | 0.839 | 0.8667 | 0.9292 |
Nucleus | 0.0431 | 0.0703 | 0.0668 | 0.0747 | 0.0746 | 0.0476 | 0.0784 | 0.1107 | 0.0894 | 0.1163 | 0.0731 | 0.0648 | 0.0815 | 0.1188 | 0.0793 | 0.0508 | 0.0581 | 0.0696 |
Peroxisomes | 0.0019 | 0 | 0.0001 | 0.0053 | 0.0018 | 0.0007 | 0.0018 | 0 | 0.002 | 0 | 0.0043 | 0.0001 | 0.0059 | 0 | 0.0001 | 0.0056 | 0.0003 | 0 |
Punctate Nuclear | 0.0015 | 0.0001 | 0.0002 | 0.0012 | 0.0012 | 0.0012 | 0.0246 | 0.0001 | 0.0071 | 0.0074 | 0.0016 | 0.0011 | 0.0032 | 0.0005 | 0.0002 | 0.0245 | 0.0029 | 0.0005 |
Vacuole | 0.012 | 0.0002 | 0.0002 | 0.0009 | 0.0004 | 0.0005 | 0.001 | 0.0007 | 0.0005 | 0.0024 | 0.0023 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0012 | 0.0012 | 0.0001 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0001 | 0.0018 | 0.0003 | 0.0004 | 0.0013 | 0.0003 | 0.001 | 0.0007 | 0.003 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0027 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.8329 | 36.7594 | 40.5367 | 39.5088 | 43.3182 | 29.111 | 30.7147 | 25.9565 | 24.7339 | 44.3916 |
Translational Efficiency | 1.9828 | 1.6644 | 1.5487 | 1.3482 | 1.4373 | 2.0231 | 1.2965 | 1.1092 | 1.2047 | 1.1992 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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