Standard name
Human Ortholog
Description DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.16 0.34 0.32 0.2 0.27 0.21 – 0.16 0.16 0.18 0.15 0.29 0.37 0.41 0.27 0.22 0.35 0.08 0 0.14 0.16 0.23 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Mitochondria 0.28 0 0 0.11 0 0.22 0.28 – 0.59 0.46 0.72 0.45 0 0 0 0 0.05 0.09 0 0 0 0.05 0 0
Nucleus 0.6 0.54 0.46 0.5 0.57 0.48 0.57 – 0.47 0.56 0.41 0.58 0.6 0.57 0.48 0.32 0.4 0.26 0.59 0.64 0.31 0.42 0.32 0.3
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.48 0.19 0.13 0.27 0.15 0.08 – 0.05 0.07 0.07 0.07 0.16 0.13 0.14 0.36 0.33 0.35 0.27 0.26 0.38 0.27 0.37 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 1 0 1 1 0 0 0 0 0 0 0 2 0 0 1
Bud 0 0 0 0 0 1 4 – 2 3 0 6 1 0 0 0 0 0 0 0 7 1 3 5
Bud Neck 0 0 0 0 1 0 3 – 1 2 1 1 1 0 0 0 0 0 0 0 1 0 1 3
Bud Site 0 0 0 0 0 2 1 – 10 2 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 20 13 37 48 39 67 89 – 38 53 38 47 18 61 70 34 18 16 10 3 23 13 56 46
Endoplasmic Reticulum 0 0 1 0 0 0 1 – 1 0 1 2 1 4 0 12 4 1 0 0 1 0 0 0
Endosome 0 0 2 2 1 1 6 – 1 1 0 2 0 1 3 9 7 4 0 0 2 2 1 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 2 3 0 0 1 0 0 1
Mitochondria 34 3 0 17 5 54 118 – 139 157 154 143 0 2 1 4 4 4 3 2 2 4 5 6
Nucleus 72 44 50 75 111 119 238 – 112 190 88 182 38 95 81 41 33 12 84 70 52 35 78 68
Nuclear Periphery 0 0 0 0 0 0 1 – 0 1 2 2 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 4 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 2 1 3 – 2 4 3 5 1 0 0 0 1 0 2 1 3 1 4 10
Vac/Vac Membrane 29 39 21 20 53 38 35 – 11 25 14 22 10 22 24 46 27 16 39 28 65 22 91 76
Unique Cell Count 120 81 108 150 194 248 420 236 340 214 316 63 167 169 127 83 46 143 111 173 85 249 226
Labelled Cell Count 155 99 114 164 212 284 499 317 440 303 417 71 185 180 152 97 56 143 111 173 85 249 226


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.1 5.5 5.3 5.7 4.4 4.6 – 3.7 4.1 3.8 4.0 5.7 5.4 5.6 7.4 7.4 7.5 5.3 5.2 5.5
Std Deviation (1e-4) 0.4 0.9 1.2 1.9 1.5 1.0 1.4 – 0.7 0.7 1.7 1.1 1.0 1.1 1.2 2.3 2.7 2.7 1.3 1.1 1.3
Intensity Change (Log2) – – – -0.07 0.05 -0.33 -0.25 – -0.59 -0.42 -0.53 -0.48 0.05 -0.04 0.02 0.42 0.42 0.43 -0.05 -0.09 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -2.7 -1.4 -2.8 – -3.8 -4.2 -3.3 -4.4 -0.8 0.4 1.2 -1.2 -1.9 0.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.8 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 1.9 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0.6 1.8 0.3 1.9 – 0.2 1.7 -0.9 2.0 1.8 1.7 0.3 -2.2 -0.9 -2.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 1.5 -1.0 -3.3 – -4.4 -3.6 -3.5 -3.7 -0.6 -1.4 -1.2 2.8 2.1 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5745 -0.3848 -0.3823 -0.7589 -0.6608 -0.5811 -0.0438 0.1681 -0.2043 0.1528 -0.0202 -0.034 0.5408 0.2187 0.0245 0.0627 0.6207 -0.2175
Actin 0.0198 0.0003 0.0018 0.0005 0.0082 0.0005 0.0122 0.0004 0.0159 0.0069 0.015 0.0046 0.0251 0.0118 0.0182 0.0139 0.0285 0.0115
Bud 0.0005 0.0005 0.0005 0.0001 0.004 0.0001 0.0006 0.0002 0.0009 0.0009 0.0013 0.0001 0.0011 0.0001 0.0014 0.003 0.0009 0.0002
Bud Neck 0.0136 0.0005 0.0009 0.0009 0.0023 0.0078 0.0055 0.0007 0.0025 0.0046 0.0013 0.0042 0.0218 0.0004 0.004 0.0011 0.0128 0.0046
Bud Periphery 0.0007 0.0002 0.0022 0.0001 0.0021 0.0002 0.0011 0.0002 0.0049 0.0027 0.0046 0.0002 0.0014 0.0001 0.0018 0.0118 0.0017 0.0003
Bud Site 0.0195 0.0122 0.0045 0.0002 0.0015 0.0002 0.0113 0.0019 0.0026 0.0037 0.0013 0.0003 0.0172 0.0009 0.0057 0.0017 0.0024 0.0005
Cell Periphery 0.0005 0.0002 0.0008 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0007 0.0002 0.0001 0.0008 0.0001 0.0003 0.0005 0.0003 0.0001
Cytoplasm 0.1124 0.1206 0.123 0.1135 0.0883 0.158 0.0989 0.1134 0.0865 0.0481 0.0912 0.0873 0.1007 0.126 0.1415 0.0608 0.1085 0.0818
Cytoplasmic Foci 0.0381 0.0038 0.0092 0.001 0.0266 0.0038 0.0119 0.0033 0.0083 0.0216 0.019 0.0028 0.0229 0.0107 0.0111 0.0082 0.0259 0.0039
Eisosomes 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0005 0.0001 0.0002 0.0003 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0108 0.0062 0.008 0.0083 0.0056 0.0041 0.007 0.0033 0.0051 0.0031 0.0043 0.0016 0.0131 0.0057 0.0066 0.008 0.0082 0.0042
Endosome 0.0391 0.0055 0.0143 0.0021 0.081 0.0141 0.0206 0.003 0.0102 0.0735 0.0438 0.0026 0.042 0.0096 0.0184 0.0179 0.075 0.0069
Golgi 0.0103 0.0001 0.0046 0 0.0331 0.0032 0.0038 0.0001 0.0055 0.0355 0.0213 0.0014 0.0092 0.0019 0.005 0.0025 0.057 0.0021
Lipid Particles 0.0341 0.0008 0.0128 0.0001 0.0417 0.0178 0.0142 0.0005 0.0034 0.0252 0.024 0.0076 0.0172 0.0011 0.0082 0.0026 0.0339 0.0017
Mitochondria 0.0057 0.0005 0.0113 0.0008 0.014 0.0165 0.0121 0.001 0.0074 0.0301 0.1214 0.0068 0.006 0.0005 0.0036 0.0133 0.0273 0.0019
None 0.1185 0.1739 0.1448 0.1114 0.2639 0.0968 0.1468 0.163 0.183 0.1678 0.0872 0.1235 0.051 0.1679 0.0785 0.0985 0.1064 0.058
Nuclear Periphery 0.0426 0.0301 0.0421 0.0308 0.0137 0.0136 0.0421 0.0199 0.0373 0.0267 0.02 0.0123 0.0278 0.0291 0.0254 0.0249 0.0333 0.0206
Nucleolus 0.0042 0.007 0.0067 0.0027 0.0054 0.0051 0.0075 0.0099 0.005 0.0078 0.0039 0.0077 0.0082 0.0032 0.0039 0.007 0.0033 0.0063
Nucleus 0.4759 0.5937 0.5681 0.7167 0.3486 0.6379 0.5473 0.6324 0.5718 0.4954 0.4902 0.691 0.5916 0.608 0.6218 0.7013 0.4111 0.7551
Peroxisomes 0.0254 0.0001 0.0028 0.0001 0.0172 0.0012 0.0144 0.0006 0.0094 0.0071 0.0203 0.0011 0.0102 0.0002 0.0065 0.0012 0.0232 0.0014
Punctate Nuclear 0.0211 0.0347 0.0359 0.0083 0.0362 0.0148 0.034 0.0428 0.0352 0.0235 0.0237 0.0436 0.019 0.0188 0.0317 0.0132 0.0319 0.0371
Vacuole 0.0054 0.0082 0.0041 0.0017 0.0042 0.0026 0.0056 0.0026 0.0035 0.0092 0.0036 0.0009 0.0115 0.0032 0.0052 0.0049 0.0051 0.0013
Vacuole Periphery 0.0015 0.0009 0.0012 0.0005 0.0017 0.0015 0.0025 0.0004 0.001 0.0055 0.0025 0.0004 0.0018 0.0007 0.0011 0.0037 0.003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3915 5.7136 4.6252 7.0994 6.0268 4.6458 7.3526 9.7477 9.7139 7.6919
Translational Efficiency 0.8276 0.6534 0.8607 0.5509 0.5273 1.1157 0.8749 0.4523 0.7147 0.5602

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
Localization
Cell Percentages nucleus (33%), cytoplasm (4%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad30

Rad30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available