ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.05 0.05 0.05 0 0 0.05 0.05 0.05 0.07 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0.07 0 0.06 0 0.05 0.06 0.05 0.06 0.07 0.14 0.21 0.16 0.09 0.17 0 0 0 0 0 0
Cytoplasm 0.18 0.22 0.22 0.23 0.22 0.23 0.16 0.22 0.24 0.2 0.23 0.15 0.12 0.1 0.1 0.44 0.5 0.43 0 0.06 0.06 0 0 0
Endoplasmic Reticulum 0.78 0.77 0.6 0.62 0.5 0.35 0.38 0.13 0.39 0.4 0.27 0.36 0.78 0.81 0.76 0.07 0.05 0 0.84 0.8 0.76 0.74 0.73 0.72
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0.17 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.22 0 0.06 0.08 0 0.22 0.14 0 0 0 0.1 0.08 0.1 0.05 0 0.07 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0.26 0.23 0.35 0.3 0.51 0.61 0.32 0.42 0.33 0.37 0.11 0.08 0.08 0.35 0.36 0.42 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 7 5 9 18 11 9 9 14 14 14 26 8 26 39 5 3 3 4 8 1 9 24 23
Bud 0 0 1 1 1 6 4 15 7 4 7 7 1 2 3 0 1 0 0 2 0 2 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 6 4
Bud Site 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 6 5 8 25 8 25 9 15 18 13 23 24 74 126 20 11 14 0 0 3 11 16 17
Cytoplasm 28 42 22 44 79 52 69 102 66 56 63 60 39 53 60 56 65 35 4 11 7 21 24 27
Endoplasmic Reticulum 121 148 60 120 181 80 158 61 108 113 74 144 255 446 448 9 7 2 136 167 99 346 469 439
Endosome 1 0 1 2 3 1 3 7 2 1 2 2 0 0 0 1 3 1 1 1 0 2 2 9
Golgi 0 0 0 2 9 0 3 0 1 2 0 2 7 6 12 16 11 14 1 1 0 3 1 4
Mitochondria 3 4 0 4 6 50 17 28 23 10 61 57 2 9 1 13 10 8 8 4 9 29 33 29
Nucleus 0 3 2 0 2 3 5 11 5 2 5 6 1 1 2 4 2 1 0 1 0 1 0 0
Nuclear Periphery 3 2 0 0 1 2 3 0 4 2 3 11 9 5 3 0 0 0 0 1 0 12 11 4
Nucleolus 0 0 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0
Vac/Vac Membrane 17 7 26 44 127 69 216 285 87 118 91 147 35 44 49 45 46 34 1 4 4 20 31 36
Unique Cell Count 156 191 100 194 364 230 420 469 274 280 274 400 326 548 593 128 129 81 163 209 130 470 641 613
Labelled Cell Count 179 219 123 234 452 283 512 528 332 340 335 486 381 667 743 169 160 112 163 209 130 470 641 613


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.0 7.9 8.6 9.9 7.3 9.6 10.9 10.2 9.6 11.1 9.5 12.7 15.9 17.8 11.7 10.9 12.9 7.8 8.0 8.4
Std Deviation (1e-4) 1.4 1.8 1.8 1.6 1.9 2.8 1.8 13.3 11.8 1.8 14.0 1.9 3.1 3.9 4.1 3.1 2.7 3.3 1.9 2.3 2.4
Intensity Change (Log2) – – – 0.13 0.33 -0.1 0.29 0.47 0.38 0.28 0.49 0.28 0.7 1.01 1.18 0.58 0.48 0.72 -0.01 0.02 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.6 0 0 0.6 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.7 0 0.4 0 0.2 0.5 0 0.3 0.8 2.4 3.8 2.5 1.0 2.7
Cytoplasm 0.1 -0.1 0.1 -1.3 -0.1 0.4 -0.4 0.2 -1.7 -2.5 -3.5 -3.4 3.4 4.4 3.1
Endoplasmic Reticulum 0.3 -1.8 -4.3 -4.1 -10.4 -3.5 -3.4 -5.9 -4.4 3.6 4.8 3.3 -8.6 -9.0 -8.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0 4.3
Mitochondria 0 0 5.1 0 0 3.0 0 5.2 4.0 0 0 0 3.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 1.7 0.7 4.6 6.3 1.1 2.9 1.3 2.0 -3.8 -5.3 -5.3 1.5 1.6 2.3