Standard name | |
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Human Ortholog | |
Description | Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 5 |
Bud | 0 | 0 | 0 | 2 | 0 | 8 | 0 | – | 2 | 3 | 3 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 0 | 3 | 15 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 5 | 2 | – | 6 | 4 | 8 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 7 | 18 | 25 | 45 | 66 | 92 | – | 59 | 50 | 43 | 51 | 36 | 111 | 120 | 59 | 51 | 39 | 2 | 5 | 7 | 7 | 14 | 21 |
Endoplasmic Reticulum | 0 | 3 | 2 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 16 | 8 | 12 | 0 | 0 | 0 | 0 | 3 | 6 |
Endosome | 3 | 0 | 1 | 11 | 9 | 1 | 23 | – | 1 | 4 | 2 | 12 | 3 | 5 | 11 | 16 | 14 | 2 | 5 | 5 | 2 | 4 | 25 | 32 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 1 | 0 | 2 | 0 | 4 |
Mitochondria | 21 | 23 | 1 | 27 | 7 | 160 | 100 | – | 199 | 194 | 240 | 271 | 11 | 1 | 4 | 6 | 4 | 4 | 3 | 4 | 3 | 4 | 17 | 16 |
Nucleus | 210 | 233 | 194 | 232 | 190 | 121 | 282 | – | 192 | 248 | 263 | 262 | 221 | 386 | 419 | 129 | 85 | 81 | 213 | 235 | 171 | 107 | 253 | 175 |
Nuclear Periphery | 5 | 0 | 0 | 3 | 0 | 0 | 0 | – | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 7 | 9 |
Nucleolus | 23 | 3 | 0 | 2 | 0 | 3 | 2 | – | 0 | 2 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 1 | 28 | 22 | 12 | 13 | 33 | 25 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 5 | 2 | 2 | 1 | 20 | – | 2 | 9 | 3 | 10 | 0 | 1 | 3 | 2 | 2 | 0 | 7 | 7 | 11 | 7 | 28 | 33 |
Vac/Vac Membrane | 18 | 59 | 14 | 13 | 46 | 15 | 66 | – | 28 | 22 | 33 | 36 | 14 | 46 | 94 | 110 | 96 | 79 | 33 | 43 | 40 | 57 | 184 | 163 |
Unique Cell Count | 254 | 304 | 228 | 285 | 281 | 284 | 515 | 381 | 417 | 477 | 516 | 273 | 534 | 609 | 294 | 216 | 192 | 313 | 335 | 259 | 218 | 606 | 535 | |
Labelled Cell Count | 297 | 328 | 235 | 317 | 300 | 381 | 589 | 491 | 537 | 598 | 671 | 287 | 557 | 652 | 339 | 262 | 218 | 313 | 335 | 259 | 218 | 606 | 535 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.4 | 5.6 | 5.5 | 6.0 | 3.8 | 4.9 | – | 4.1 | 4.3 | 4.2 | 4.3 | 5.8 | 6.2 | 6.0 | 6.3 | 6.8 | 6.5 | 5.5 | 5.6 | 5.7 |
Std Deviation (1e-4) | 0.9 | 0.7 | 0.8 | 1.5 | 1.6 | 1.5 | 1.5 | – | 1.4 | 1.1 | 0.8 | 0.8 | 1.3 | 1.6 | 1.8 | 1.3 | 1.5 | 1.4 | 2.1 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.09 | -0.55 | -0.2 | – | -0.45 | -0.38 | -0.43 | -0.38 | 0.04 | 0.13 | 0.1 | 0.17 | 0.28 | 0.21 | -0.03 | -0.02 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 2.8 | 4.7 | 3.5 | – | 2.7 | 1.6 | 0.5 | 0.9 | 1.9 | 4.3 | 4.1 | 3.9 | 4.6 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 3.1 |
Endosome | 2.5 | 0 | 0 | 2.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.5 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.5 | 0 | 0 | 7.0 | – | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.1 | -4.6 | -9.8 | -7.9 | – | -8.6 | -6.7 | -7.8 | -8.9 | -1.2 | -3.8 | -4.7 | -9.6 | -10.0 | -9.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | 3.6 | -0.4 | 2.7 | – | 0.6 | -0.5 | 0.4 | 0.4 | -0.5 | 1.2 | 3.6 | 8.3 | 9.3 | 8.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8972 | 0.7195 | 0.3944 | 0.4396 | 0.5082 | 0.4515 | 1.0456 | 1.6173 | 1.3302 | 1.234 | 1.0088 | 1.3975 | 4.3015 | 4.7387 | 4.3013 | 4.4468 | 3.773 | 4.1702 |
Actin | 0.0391 | 0.0002 | 0.0286 | 0.0015 | 0.0084 | 0.0043 | 0.0065 | 0.0001 | 0.0076 | 0.0013 | 0.0072 | 0 | 0.0306 | 0.0014 | 0.0044 | 0.0014 | 0.0065 | 0.0008 |
Bud | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0046 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0001 |
Bud Neck | 0.0016 | 0.001 | 0.0021 | 0.0009 | 0.0729 | 0.0088 | 0.001 | 0.0008 | 0.0008 | 0.0011 | 0.0012 | 0.0027 | 0.0038 | 0.0022 | 0.0041 | 0.0014 | 0.0017 | 0.004 |
Bud Periphery | 0.0009 | 0.0003 | 0.0014 | 0.0004 | 0.0073 | 0.0025 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0013 | 0.0007 | 0.001 | 0.0003 |
Bud Site | 0.0018 | 0.0019 | 0.0025 | 0.0004 | 0.0142 | 0.001 | 0.0003 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0026 | 0.0033 | 0.0025 | 0.0008 | 0.0004 | 0.0004 |
Cell Periphery | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.03 | 0.0154 | 0.0078 | 0.017 | 0.0065 | 0.0109 | 0.008 | 0.0064 | 0.0043 | 0.0229 | 0.001 | 0.0029 | 0.0042 | 0.0123 | 0.0065 | 0.0055 | 0.0163 | 0.0024 |
Cytoplasmic Foci | 0.0202 | 0.0021 | 0.002 | 0.005 | 0.0061 | 0.0026 | 0.0004 | 0.0001 | 0.0001 | 0.0014 | 0.0015 | 0.0013 | 0.0053 | 0.0004 | 0.0044 | 0.0002 | 0.0062 | 0.0003 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0044 | 0.0025 | 0.0038 | 0.0127 | 0.0014 | 0.0021 | 0.0017 | 0.0003 | 0.0004 | 0.0014 | 0.0008 | 0.0005 | 0.0042 | 0.0019 | 0.0035 | 0.001 | 0.0012 | 0.0003 |
Endosome | 0.0465 | 0.0059 | 0.0131 | 0.0212 | 0.008 | 0.0233 | 0.0035 | 0.0003 | 0.0008 | 0.0043 | 0.0074 | 0.0006 | 0.0125 | 0.003 | 0.0095 | 0.0033 | 0.0148 | 0.0012 |
Golgi | 0.0171 | 0.0003 | 0.0025 | 0.0035 | 0.0094 | 0.0134 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0021 | 0 | 0.0039 | 0.0002 | 0.0022 | 0.0017 | 0.0157 | 0.0001 |
Lipid Particles | 0.0105 | 0.0033 | 0.0016 | 0.0066 | 0.0052 | 0.005 | 0.0022 | 0.0001 | 0.0001 | 0.0006 | 0.0064 | 0.0001 | 0.008 | 0.0001 | 0.0025 | 0.0048 | 0.0232 | 0.0003 |
Mitochondria | 0.0065 | 0.0011 | 0.0063 | 0.0571 | 0.053 | 0.0034 | 0.0006 | 0.0003 | 0.0007 | 0.0014 | 0.004 | 0.0002 | 0.0021 | 0.0005 | 0.0034 | 0.0223 | 0.0144 | 0.0025 |
None | 0.022 | 0.0135 | 0.0111 | 0.005 | 0.0018 | 0.0127 | 0.0052 | 0.0128 | 0.0015 | 0.0357 | 0.0037 | 0.0019 | 0.0023 | 0.0023 | 0.0157 | 0.0011 | 0.0068 | 0.0047 |
Nuclear Periphery | 0.038 | 0.0357 | 0.0302 | 0.0648 | 0.0159 | 0.0264 | 0.0307 | 0.008 | 0.0118 | 0.0439 | 0.0067 | 0.0032 | 0.0204 | 0.0314 | 0.005 | 0.0243 | 0.0062 | 0.0053 |
Nucleolus | 0.05 | 0.0808 | 0.1188 | 0.0621 | 0.094 | 0.0838 | 0.0482 | 0.069 | 0.079 | 0.0987 | 0.0658 | 0.0508 | 0.0567 | 0.0535 | 0.0744 | 0.0577 | 0.027 | 0.0667 |
Nucleus | 0.6712 | 0.82 | 0.7355 | 0.7265 | 0.6725 | 0.7779 | 0.8797 | 0.893 | 0.8837 | 0.7645 | 0.8759 | 0.9187 | 0.8144 | 0.8693 | 0.8363 | 0.8631 | 0.8036 | 0.8997 |
Peroxisomes | 0.0027 | 0.0003 | 0.0034 | 0.0003 | 0.0082 | 0.0005 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0032 | 0 | 0.013 | 0.0001 | 0.0013 | 0.0006 | 0.0055 | 0.0001 |
Punctate Nuclear | 0.0304 | 0.007 | 0.0221 | 0.0103 | 0.0035 | 0.0149 | 0.0086 | 0.0066 | 0.0061 | 0.0194 | 0.0114 | 0.0148 | 0.0094 | 0.01 | 0.014 | 0.0053 | 0.0381 | 0.0094 |
Vacuole | 0.0042 | 0.0066 | 0.0038 | 0.0026 | 0.0044 | 0.0045 | 0.0018 | 0.0008 | 0.0014 | 0.0012 | 0.0003 | 0.0013 | 0.0024 | 0.0062 | 0.0071 | 0.0022 | 0.0069 | 0.001 |
Vacuole Periphery | 0.0019 | 0.0015 | 0.0021 | 0.0016 | 0.0021 | 0.0012 | 0.0009 | 0.0002 | 0.0003 | 0.0011 | 0.0005 | 0.0002 | 0.0008 | 0.0014 | 0.0006 | 0.0021 | 0.0039 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.3171 | 8.2611 | 9.8082 | 8.2194 | 13.1798 | 3.6945 | 7.0999 | 10.7114 | 8.2398 | 11.1036 |
Translational Efficiency | 1.5961 | 1.5815 | 1.2469 | 1.9149 | 1.1717 | 3.0532 | 1.8665 | 0.8535 | 1.2314 | 1.3706 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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