Standard name
Human Ortholog
Description Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.21 0.21 0.26 0.14 0.15 0.12
Golgi 0.24 0.4 0.1 0.17 0.43 0.19 – 0.38 0.19 0.2 0.06 0.08 0.32 0.18 0.27 0.39 0.43 0.39 0.26 0.48 0.19 0.26 0.31 0.33
Mitochondria 0.13 0.35 0.32 0.78 0.52 0.73 – 0.54 0.7 0.76 0.82 0.81 0.29 0.35 0.2 0.07 0.1 0.07 0 0.08 0.05 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.16 0.2 0.29 0.25 0.28 – 0.29 0.29 0.21 0.25 0.25 0.28 0.28 0.29 0.33 0.34 0.35 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.76 0.35 0.68 0.17 0.23 0.09 – 0.11 0.09 0.1 0.08 0.1 0.68 0.65 0.74 0.59 0.76 0.7 0.42 0.16 0.41 0.49 0.45 0.47
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 2 5 5 7 – 19 14 13 8 8 0 3 3 3 1 0 3 2 1 1 1 2
Bud 0 2 0 1 0 1 – 2 0 0 3 4 0 0 0 0 0 0 2 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 4 5 8 7 10 – 9 6 5 5 10 3 6 11 12 2 8 0 1 0 0 0 0
Endoplasmic Reticulum 1 3 1 2 3 5 – 16 7 10 4 4 1 1 3 10 5 4 1 0 2 0 2 0
Endosome 0 1 5 0 1 4 – 6 1 0 0 1 2 1 1 1 0 0 79 23 66 18 34 30
Golgi 83 38 25 40 95 57 – 167 48 60 16 27 78 64 102 50 45 48 99 52 48 34 68 84
Mitochondria 46 33 77 186 115 219 – 239 181 227 224 267 69 126 74 9 10 9 13 8 13 5 7 8
Nucleus 3 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 2 0 0 1 1 0
Nuclear Periphery 0 1 2 1 0 0 – 2 1 1 0 0 4 1 0 0 1 0 4 0 10 1 2 2
Nucleolus 59 15 47 69 56 84 – 127 76 63 69 82 68 101 110 42 35 43 5 0 1 1 2 0
Peroxisomes 0 0 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
SpindlePole 0 0 1 0 0 1 – 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 266 33 165 42 51 28 – 48 24 29 23 34 164 230 276 76 79 85 159 17 104 64 99 120
Unique Cell Count 349 95 241 240 223 301 442 258 299 273 328 241 356 373 128 104 122 377 109 253 130 222 258
Labelled Cell Count 468 135 330 355 334 417 635 359 409 352 440 389 533 580 204 178 197 377 109 253 130 222 258


Lipid Particles, Punctate Nuclear, Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.2 46.9 26.5 35.5 38.1 35.5 – 35.2 35.2 33.0 31.9 30.3 44.8 48.6 48.3 63.1 63.6 63.3 41.2 45.1 46.4
Std Deviation (1e-4) 12.2 19.8 8.2 9.5 10.5 10.5 – 10.1 9.9 9.2 9.5 9.0 13.0 13.4 13.2 26.7 22.5 23.2 15.1 13.7 15.3
Intensity Change (Log2) – – – 0.42 0.52 0.42 – 0.41 0.41 0.32 0.27 0.19 0.76 0.88 0.87 1.25 1.26 1.26 0.64 0.77 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 2.5 2.9 2.5 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.7 0.9 – 0 0.2 0 0 0.7 0 0 0.7 3.2 0 0
Endoplasmic Reticulum 0 0 0 – 2.6 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.0 7.9 2.8 – 7.6 2.6 3.1 -1.9 -0.9 5.9 2.6 5.1 6.5 7.0 6.5
Mitochondria 10.0 4.3 9.5 – 5.5 8.5 10.2 11.5 11.9 -0.8 0.9 -3.4 -5.4 -4.4 -5.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.4 1.5 2.3 – 2.6 2.6 0.4 1.6 1.5 2.2 2.5 2.8 2.8 2.8 3.3
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -11.3 -9.8 -14.3 – -15.5 -13.6 -14.1 -14.1 -14.4 -0.1 -1.0 1.5 -1.7 1.4 0.2