Standard name
Human Ortholog
Description Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0.23 – 0.05 0.09 0.1 0.12 0.13 0.12 0.13
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 – 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0.14 0.07 0.08 0.06 0 0 0 – 0.05 0 0 0.23 0.24 0.44 0.53 – 0.47 0.11 0.17 0.07 0.17 0.19 0.19
Mitochondria 0.94 0.91 0.88 0.91 0.84 0.89 0.89 0.84 – 0.85 0.92 0.94 0.79 0.81 0.76 0.05 – 0.05 0.72 0.63 0.67 0.51 0.5 0.51
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Nucleolus 0.09 0 0.08 0.05 0.13 0.22 0.21 0.25 – 0.16 0.17 0.14 0 0 0 0.18 – 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0.06 0 0 – 0.05 0 0 0.1 0.12 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0 0 0 0 0 0 – 0 0 0 0.11 0.06 0.06 0.1 – 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 22 8 2 1 3 1 2 – 3 1 1 16 20 16 14 – 1 66 68 41 39 57 54
Bud 2 1 5 1 1 1 5 0 – 2 9 4 1 1 3 0 – 1 3 6 0 1 4 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 – 1 1 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 5 13 4 6 1 1 1 1 – 0 0 0 10 7 6 4 – 1 0 1 0 0 0 1
Cytoplasm 1 4 2 1 5 4 7 18 – 8 8 8 5 1 6 1 – 4 0 0 0 0 0 0
Endoplasmic Reticulum 2 0 6 2 3 9 7 4 – 13 7 3 1 5 1 1 – 1 0 1 0 0 1 0
Endosome 0 0 0 0 1 0 0 0 – 0 0 0 2 3 2 0 – 0 7 11 3 6 8 5
Golgi 29 81 20 16 19 9 7 17 – 19 11 8 96 90 178 33 – 9 83 114 24 53 86 77
Mitochondria 621 543 269 172 257 325 434 390 – 338 395 423 323 309 305 3 – 1 528 423 223 160 233 211
Nucleus 1 1 0 0 1 3 3 0 – 0 2 0 1 0 0 1 – 0 0 0 0 0 0 0
Nuclear Periphery 12 11 5 4 9 5 7 1 – 7 10 10 5 2 4 0 – 1 2 2 0 1 4 2
Nucleolus 62 26 23 9 41 81 105 116 – 64 74 62 17 13 14 11 – 2 1 4 1 4 5 5
Peroxisomes 10 8 17 7 3 1 4 1 – 5 2 1 18 21 13 1 – 1 30 22 34 37 48 35
SpindlePole 1 0 0 0 1 0 3 1 – 0 0 1 0 2 1 1 – 1 0 2 2 1 2 0
Vac/Vac Membrane 38 33 2 3 13 4 13 7 – 13 7 11 47 24 26 6 – 1 3 7 1 4 5 12
Unique Cell Count 664 595 304 190 307 366 489 463 397 428 452 411 381 403 62 19 730 669 336 312 463 412
Labelled Cell Count 792 743 361 223 356 446 598 560 473 527 533 542 498 575 76 24 730 669 336 312 463 412


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.8 28.5 22.9 22.4 22.9 24.4 23.7 27.1 – 29.9 30.2 30.1 27.1 25.2 24.3 29.3 – 25.0 33.7 32.9 34.6
Std Deviation (1e-4) 8.0 7.0 8.5 7.9 8.9 9.3 10.5 12.0 – 14.3 13.7 13.8 6.4 6.4 5.7 8.1 – 10.2 12.5 13.2 12.9
Intensity Change (Log2) – – – -0.03 -0.0 0.09 0.05 0.25 – 0.38 0.4 0.39 0.25 0.14 0.09 0.36 – 0.13 0.56 0.52 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0.9 1.7 1.0 0 – 0
Bud 0 0 0 0 0 – 0 0.4 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 1.1 0 0 0 – 0
Cytoplasm 0 0 0 0 2.7 – 0 0 0 0 0 0 0 – 0
Endoplasmic Reticulum 0 0 0.4 0 0 – 1.1 -0.3 0 0 0 0 0 – 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Golgi 0.8 -0.2 -2.6 -3.9 -1.8 – -1.0 -2.7 -3.4 6.0 6.0 11.0 9.5 – 0
Mitochondria 0.7 -1.7 0.1 0.1 -1.7 – -1.3 1.7 2.5 -3.5 -2.6 -4.3 -13.7 – -9.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 1.1 0 0 0 – 0.1 0.7 0.5 0 0 0 0 – 0
Nucleolus -1.2 2.3 5.2 5.2 6.2 – 3.4 3.8 2.6 -2.0 -2.4 -2.4 2.5 – 0
Peroxisomes -1.0 -3.2 -4.2 -4.1 -4.8 – -3.3 -4.3 -4.7 -0.7 0 -1.5 0 – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Vacuole 0 2.9 0 2.0 0 – 2.4 0 1.8 5.6 3.8 3.9 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8512 -0.5 -0.5989 -0.6307 -0.6448 -0.5289 1.7879 2.7817 2.5427 1.955 2.2033 2.8898 0.5572 -0.5236 -0.7281 0.1304 -1.2834 -0.3979
Actin 0.0026 0.0003 0.0004 0.0003 0.0015 0.0003 0.0006 0.0004 0.0005 0.0003 0.0002 0.0006 0.0496 0 0 0.0523 0 0.0001
Bud 0.0059 0.002 0.0042 0.0004 0.0001 0.0036 0.0004 0.0033 0.0056 0.0065 0.0013 0.0012 0.0009 0 0 0.0003 0 0
Bud Neck 0.0073 0.0001 0.0013 0.0097 0.0094 0.0035 0.0008 0.0023 0.0005 0.0006 0.0006 0.0011 0.0199 0 0 0.0011 0 0
Bud Periphery 0.0017 0.0003 0.0013 0.0002 0.0001 0.0013 0.0008 0.0036 0.0085 0.0047 0.0028 0.0034 0.0017 0 0 0.0007 0 0
Bud Site 0.0247 0.0077 0.0093 0.0065 0.0008 0.0006 0.0077 0.0138 0.0036 0.003 0.0011 0.0001 0.0171 0 0 0.0007 0 0
Cell Periphery 0.0007 0.0001 0.0001 0 0.0001 0.0001 0.0016 0.0055 0.0024 0.0004 0.0005 0.0013 0.0032 0 0 0.0001 0 0
Cytoplasm 0.0125 0.0091 0.0058 0.005 0.0071 0.0132 0.0199 0.0327 0.0244 0.0233 0.0117 0.0362 0.0173 0.0266 0.0192 0.0149 0.0565 0.0572
Cytoplasmic Foci 0.005 0.0031 0.0053 0.003 0.0082 0.0034 0.0033 0.0043 0.0045 0.0036 0.0014 0.0018 0.0164 0.0019 0.0005 0.0061 0.0012 0.002
Eisosomes 0.0004 0.0002 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0022 0.0001 0.0002 0.0004 0 0.0001
Endoplasmic Reticulum 0.0016 0.0002 0.0002 0.0001 0.0001 0.0001 0.0165 0.0419 0.026 0.002 0.0355 0.0241 0.025 0.0003 0.0002 0.0011 0.0007 0.0002
Endosome 0.0011 0.0002 0.0001 0.0001 0.0011 0.0001 0.0014 0.0013 0.0021 0.0009 0.0035 0.0025 0.0256 0.0001 0 0.0068 0.0002 0.0001
Golgi 0.0002 0 0 0.0001 0.0009 0 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0068 0 0 0.0127 0 0
Lipid Particles 0.0007 0.0001 0.0002 0.0002 0.0046 0.0001 0.001 0.0009 0.0018 0.0005 0.0004 0.0006 0.0124 0.0002 0.0001 0.029 0.0001 0.0001
Mitochondria 0.001 0.0006 0.0003 0.0002 0.0083 0.0003 0.0003 0.0005 0.001 0.0004 0.0001 0.0002 0.0052 0 0 0.002 0 0
None 0.916 0.9656 0.9581 0.9647 0.9177 0.9695 0.9227 0.8242 0.8801 0.8984 0.9038 0.8885 0.7039 0.9705 0.9794 0.8584 0.9405 0.9357
Nuclear Periphery 0.0022 0.0011 0.0003 0.0002 0.0002 0.0002 0.0048 0.0068 0.0047 0.008 0.001 0.0058 0.0424 0.0001 0.0001 0.0016 0.0001 0.0003
Nucleolus 0.0017 0.0009 0.002 0.0006 0.0018 0.0002 0.001 0.0016 0.0025 0.0036 0.0004 0.0005 0.0102 0 0 0.0002 0 0.0001
Nucleus 0.0096 0.0039 0.0038 0.0009 0.0013 0.0011 0.0023 0.0089 0.0069 0.0213 0.0007 0.0037 0.0104 0.0001 0 0.0002 0.0001 0.0002
Peroxisomes 0.0008 0.0001 0.0002 0.0002 0.022 0.0002 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0063 0 0 0.0081 0 0.0001
Punctate Nuclear 0.0032 0.0041 0.0067 0.0069 0.0145 0.0019 0.0016 0.006 0.0043 0.0176 0.0002 0.0031 0.0129 0.0001 0 0.0025 0.0001 0.0037
Vacuole 0.001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0125 0.0401 0.0189 0.0035 0.0325 0.023 0.0066 0.0001 0.0001 0.0003 0.0003 0
Vacuole Periphery 0.0002 0.0001 0 0 0 0 0.0004 0.0015 0.0013 0.001 0.0018 0.0019 0.004 0 0 0.0003 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.2159 43.6677 60.9945 90.7411 63.3344 57.4372 57.1384 70.9938 72.7438 72.4115
Translational Efficiency 2.2088 2.7304 2.2448 1.6392 2.824 2.4655 1.9466 1.7188 2.0749 2.2398

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
Localization
Cell Percentages cytoplasm (34%), nucleus (2%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Qcr7

Qcr7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qcr7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available