Standard name
Human Ortholog
Description Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum; human homolog DDOST can complement yeast growth defect during down-regulation of yeast gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.07 0.11 0.13 0.11 0.13 0.14 0.09 – 0.16 0.21 0.2 0 0.05 0.06 0.26 0.26 0.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.06 0.05 0.05 0.07 0.06 – 0.1 0.14 0.15 0.06 0.1 0.11 0.34 0.39 0.38 0 0 0 0 0 0
Cytoplasm 0.05 0.12 0 0.05 0 0 0 0 – 0 0.07 0.06 0.06 0 0.06 0.23 0.2 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0.92 0.86 0.94 0.8 0.78 0.87 0.86 0.87 – 0.78 0.62 0.71 0.93 0.93 0.85 0 0 0.06 0.95 0.91 0.93 0.89 0.89 0.9
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0.14 0.35 0.4 0.14 0.14 0.09 – 0.05 0.07 0.06 0.06 0.07 0.05 0.12 0.21 0.24 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.08 0.07 0.05 – 0.19 0.25 0.19 0 0 0 0.18 0.15 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 19 57 47 69 96 140 98 – 158 132 178 17 25 26 25 62 15 0 5 18 14 16 13
Bud 0 4 3 0 2 4 11 7 – 13 19 10 1 0 3 6 2 0 0 1 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 36 22 33 36 71 69 – 103 86 132 27 46 51 33 94 54 0 0 8 5 21 25
Cytoplasm 4 33 14 17 25 30 26 31 – 39 45 53 29 17 29 22 47 37 0 9 0 0 4 1
Endoplasmic Reticulum 70 235 500 297 490 664 849 982 – 772 384 622 436 446 394 1 3 8 90 261 498 292 528 612
Endosome 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 4 3 73 129 248 106 142 101 – 48 46 53 30 36 24 12 50 35 0 2 0 6 8 7
Mitochondria 3 4 15 17 27 63 72 57 – 184 152 163 0 0 0 17 35 20 0 1 2 4 7 6
Nucleus 0 2 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 1 1 4 4 9 30 41 – 52 24 37 5 2 2 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 2 – 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 2 5 34 11 19 23 – 18 27 10 1 5 20 3 3 1 0 3 1 1 2 4
Unique Cell Count 76 272 534 372 625 759 991 1127 986 616 870 469 481 461 97 240 143 95 288 537 328 594 679
Labelled Cell Count 95 309 701 538 932 1020 1360 1411 1389 916 1259 546 577 549 120 297 171 95 288 537 328 594 679


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.5 26.5 23.3 23.7 23.3 20.1 18.9 19.4 – 16.5 16.2 15.9 26.2 26.0 25.9 16.3 20.0 22.5 27.0 28.1 29.8
Std Deviation (1e-4) 4.5 5.2 4.0 3.9 3.8 3.6 3.2 3.7 – 3.2 3.2 2.8 4.2 4.4 6.3 3.7 5.3 6.6 4.2 4.9 5.2
Intensity Change (Log2) – – – 0.03 0.0 -0.21 -0.3 -0.27 – -0.49 -0.52 -0.55 0.17 0.16 0.15 -0.51 -0.22 -0.05 0.21 0.27 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 0.2 1.1 1.9 -1.3 – 2.9 4.9 4.8 -4.3 -3.2 -2.9 4.1 5.4 -0.1
Bud 0 0 0 0 0 – 1.4 3.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -1.0 -1.5 0.3 -0.5 – 2.4 4.0 4.7 -0.6 1.6 2.4 7.9 11.2 9.7
Cytoplasm 1.6 1.3 1.3 0 0.2 – 1.4 3.6 3.0 2.8 0.8 2.8 7.8 8.1 9.4
Endoplasmic Reticulum -6.3 -7.3 -3.6 -4.6 -4.0 – -7.7 -12.6 -10.1 -0.4 -0.6 -4.3 -20.7 -24.9 -21.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 7.5 9.9 0.2 0.4 -2.9 – -6.1 -3.4 -4.8 -3.8 -3.2 -4.5 -0.3 2.5 3.1
Mitochondria 1.4 1.4 4.1 3.6 2.1 – 8.7 10.5 8.7 -3.7 -3.7 -3.6 0 6.2 5.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3.7 4.2 – 5.2 4.3 4.6 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 5.0 1.9 2.5 2.6 – 2.4 4.3 0 0 0 4.2 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.1042 18.6755 18.6695 6.9116 15.9994 14.7278 20.5043 21.963 21.8974 19.1021 19.4589 21.195 23.9225 22.3705 23.7706 21.463 22.8003 23.0478
Actin 0.0515 0.0032 0.004 0.1146 0.0042 0.0604 0.013 0.001 0.0176 0.0058 0.018 0.0109 0.0056 0.0238 0.0034 0.007 0.0009 0.0004
Bud 0.0028 0.0001 0.0001 0.0015 0.0005 0.0004 0.0001 0.0001 0.0001 0.0015 0.0001 0.0001 0.0001 0.0003 0.0001 0 0 0
Bud Neck 0.0014 0.0008 0.002 0.0087 0.0036 0.0077 0.0006 0.0007 0.002 0.0016 0.0009 0.0014 0.0003 0.0009 0.0005 0.0023 0.0008 0.0006
Bud Periphery 0.0028 0.0003 0.0006 0.0018 0.001 0.001 0.0001 0.0005 0.0004 0.0016 0.0001 0.0002 0.0002 0.0006 0.0001 0.0002 0.0002 0
Bud Site 0.0155 0.0009 0.0007 0.0053 0.0005 0.0004 0.0006 0.0004 0.0004 0.0163 0.0001 0.0001 0.0003 0.0024 0.0002 0 0 0
Cell Periphery 0.0059 0.0149 0.0101 0.0009 0.0053 0.0048 0.0034 0.0189 0.0076 0.0035 0.0019 0.0027 0.0054 0.0158 0.0062 0.0042 0.004 0.0016
Cytoplasm 0.0447 0.0938 0.0826 0.1568 0.046 0.0496 0.0262 0.0489 0.0116 0.0429 0.0048 0.0058 0.0079 0.0498 0.0197 0.006 0.0034 0.0055
Cytoplasmic Foci 0.0162 0.0027 0.0014 0.047 0.0098 0.0046 0.0117 0.0024 0.0034 0.0178 0.0099 0.0066 0.0015 0.0021 0.0018 0.0002 0.0011 0.0006
Eisosomes 0.0013 0.0003 0.0001 0.0011 0 0.0004 0.0005 0.0029 0.0008 0.0001 0.0002 0.0011 0.0028 0.0008 0.0005 0.0058 0.0002 0.0001
Endoplasmic Reticulum 0.5822 0.7931 0.8783 0.2228 0.7099 0.6361 0.8285 0.8506 0.8742 0.8258 0.8672 0.9182 0.9244 0.8372 0.923 0.9462 0.9452 0.9739
Endosome 0.0335 0.004 0.0018 0.0763 0.0375 0.0426 0.0184 0.0101 0.0092 0.0255 0.0074 0.0045 0.0095 0.0085 0.0066 0.0026 0.0065 0.0023
Golgi 0.013 0.0166 0.0019 0.0155 0.0034 0.0418 0.0043 0.014 0.0148 0.0063 0.0153 0.0087 0.0098 0.0129 0.0179 0.0174 0.0223 0.0065
Lipid Particles 0.0431 0.0262 0.0061 0.0106 0.0067 0.0507 0.0072 0.0141 0.0079 0.0073 0.0092 0.0077 0.0061 0.0125 0.0019 0.0008 0.003 0.0012
Mitochondria 0.0066 0.0002 0.0001 0.0557 0.0011 0.0112 0.0007 0.0149 0.0226 0.0089 0.0008 0.0044 0.0031 0.0036 0.0007 0.0008 0.0007 0.0003
None 0.0083 0.0087 0.0001 0.0898 0.0022 0.0008 0.0028 0.0006 0.0004 0.002 0.0002 0.0004 0.001 0.0002 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0321 0.0092 0.0064 0.0685 0.0177 0.0389 0.0581 0.011 0.0113 0.0161 0.0403 0.0185 0.0167 0.0182 0.0127 0.0043 0.0089 0.0055
Nucleolus 0.0018 0 0 0.0161 0.0014 0.0001 0.002 0.0002 0.0008 0.0008 0 0.0002 0.0001 0.0001 0 0 0 0
Nucleus 0.0475 0.0008 0.0005 0.059 0.0043 0.0304 0.0087 0.0007 0.0026 0.0016 0.0006 0.0024 0.0005 0.0014 0.0008 0.0002 0.0003 0.0002
Peroxisomes 0.0616 0.0182 0 0.0076 0.0002 0.0075 0.0014 0.0001 0.0002 0.0017 0.0204 0.0017 0.0002 0.0001 0 0 0 0
Punctate Nuclear 0.0139 0.0003 0.0002 0.0236 0.0005 0.003 0.0056 0.0001 0.0055 0.0006 0.0003 0.0011 0.0005 0.0003 0.0001 0 0 0
Vacuole 0.011 0.0055 0.0028 0.0047 0.1337 0.0045 0.0037 0.0065 0.0044 0.0094 0.001 0.0019 0.0022 0.0065 0.0029 0.0014 0.0015 0.0009
Vacuole Periphery 0.0034 0.0004 0.0002 0.0122 0.0105 0.0029 0.0025 0.0014 0.0022 0.0029 0.0013 0.0014 0.0018 0.0021 0.0009 0.0005 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 139.5256 135.7888 95.1004 129.6832 120.0475 104.0532 125.1476 115.8319 109.9209 147.6316
Translational Efficiency 1.3011 1.1913 1.5113 1.0791 1.2463 1.9624 1.2255 1.1838 1.3371 1.2243

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum; human homolog DDOST can complement yeast growth defect during down-regulation of yeast gene
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Wbp1

Wbp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wbp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available