Standard name
Human Ortholog
Description Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.07 0.11 0.13 0.13 0.16 0.14 0.11 0 0 0 0.07 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0.28 0.17 0.18 0.16 0.35 0.48 0.51 0.49 0.64 0.55 0.64 0.68 0.23 0.19 0.16 0.3 0.46 0.5 0.16 0.2 0.1 0.05 0.05 0
Endoplasmic Reticulum 0.6 0.8 0.65 0.58 0.36 0.31 0.33 0.39 0.24 0.29 0.21 0.2 0.67 0.72 0.73 0.09 0.14 0.09 0.46 0.42 0.52 0.41 0.32 0.32
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0.13 0.23 0.25 0.17 0.14 0.08 0.11 0.11 0.1 0.09 0.09 0.08 0.12 0.48 0.27 0.26 0.27 0.19 0.22 0.39 0.54 0.55
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 1 2 7 1 10 8 11 0 0 1 1 0 0 0 7 3 2 0 0
Bud 0 3 3 0 3 9 8 16 5 15 7 16 3 1 4 0 2 0 2 8 2 8 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 3 1 5 24 49 74 49 71 47 53 1 5 4 3 7 3 0 0 0 0 0 0
Cytoplasm 34 30 33 35 82 154 225 287 241 248 217 321 50 59 63 14 32 17 40 59 30 16 13 8
Endoplasmic Reticulum 73 137 121 127 83 100 147 228 90 129 72 94 148 222 278 4 10 3 116 126 153 122 92 123
Endosome 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 5 16 12 11 4 7
Golgi 2 0 7 10 5 1 3 2 0 2 1 1 11 11 14 2 1 2 2 1 10 5 3 5
Mitochondria 0 0 0 1 0 2 0 0 1 0 3 0 0 0 0 0 0 0 0 1 0 1 0 1
Nucleus 0 0 1 2 2 1 0 1 2 0 2 3 0 0 0 0 0 0 3 3 1 2 3 5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 3 3 1 3
Nucleolus 8 4 13 7 8 9 3 7 1 3 0 0 6 1 4 1 3 1 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0
Vac/Vac Membrane 9 1 24 51 57 54 64 46 40 51 35 41 20 25 45 22 19 9 66 58 66 119 155 211
Unique Cell Count 122 172 187 220 231 322 445 586 376 447 341 470 222 308 383 46 70 34 251 303 295 303 286 386
Labelled Cell Count 126 177 205 235 246 355 501 669 430 529 394 540 240 324 413 47 74 35 251 303 295 303 286 386


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 96.8 108.9 64.2 63.6 51.1 42.6 43.1 41.5 42.6 42.2 39.8 42.1 71.8 71.4 71.3 97.6 98.4 100.8 74.0 69.5 74.2
Std Deviation (1e-4) 24.4 26.8 19.2 17.4 17.6 16.0 14.1 12.8 13.6 13.8 12.6 12.5 21.6 20.5 20.2 28.8 29.3 29.3 20.5 21.8 22.2
Intensity Change (Log2) – – – -0.01 -0.33 -0.59 -0.57 -0.63 -0.59 -0.61 -0.69 -0.61 0.16 0.15 0.15 0.61 0.62 0.65 0.2 0.11 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.2 0 1.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.8 3.9 4.4 4.4 5.1 4.6 4.0 0 0 0 0 0 0
Cytoplasm -0.5 4.1 6.8 7.7 7.6 10.4 8.7 10.1 11.8 1.2 0.4 -0.4 1.9 4.6 4.1
Endoplasmic Reticulum -1.4 -5.9 -7.4 -7.4 -6.2 -9.4 -8.4 -9.9 -11.0 0.4 1.7 1.9 -6.8 -7.2 -6.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.4 -1.0 0 0 0 0 0 0 0 0.6 -0.1 -0.1 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.8 -1.6 -2.2 0 0 0 0 0 0 -2.0 -4.3 -3.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.7 3.0 1.2 0.5 -2.1 -0.8 -0.5 -0.9 -1.6 -1.2 -1.7 -0.4 5.3 2.7 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 114.2517 140.9982 129.7823 123.9543 121.2546 129.1022 105.3123 144.9189 132.9916 124.2206 127.5113 132.9213 73.4734 119.9213 108.0838 77.8039 58.8719 117.1746
Actin 0.0003 0 0 0 0.0081 0 0.0084 0 0.0003 0 0 0 0.1125 0 0.0002 0 0.0643 0.0074
Bud 0 0.0001 0 0 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0001 0.0018 0.0001 0.0007 0.0009 0.0015 0.0022
Bud Neck 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0004 0.0002 0.0002 0.0001 0.0001 0.0002 0.0092 0.0001 0.0009 0.0001 0.0007 0.0081
Bud Periphery 0.0002 0.0003 0.0002 0.0001 0.0003 0.0004 0.0011 0.0004 0.0008 0.0002 0.0008 0.0006 0.006 0.0003 0.0026 0.0002 0.002 0.0021
Bud Site 0.0001 0.0002 0 0 0.0001 0 0.0005 0.0001 0.0013 0 0 0 0.0035 0.0002 0.0011 0.0001 0.0024 0.0011
Cell Periphery 0.3929 0.3882 0.3872 0.3159 0.3554 0.3521 0.3173 0.374 0.3982 0.2981 0.3084 0.3219 0.2221 0.3458 0.2271 0.0896 0.1426 0.3122
Cytoplasm 0.0068 0.0161 0.0145 0.0015 0.0009 0.0205 0.0046 0.0032 0.0024 0.0009 0.001 0.0011 0.0236 0.0009 0.0085 0.2855 0.0069 0.0523
Cytoplasmic Foci 0.0009 0.0003 0.0002 0 0.0021 0.0003 0.0104 0.0002 0.0046 0.0006 0.0001 0.0003 0.0005 0.0002 0.001 0.002 0.0202 0.0017
Eisosomes 0.0002 0 0 0 0.0001 0 0.0003 0 0.0003 0 0 0 0.0017 0 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0033 0.0064 0.006 0.0008 0.001 0.0091 0.0051 0.0144 0.0062 0.0007 0.0026 0.0089 0.0067 0.0005 0.0013 0.0003 0.0124 0.0024
Endosome 0.0008 0.0003 0.0004 0.0003 0.0026 0.0015 0.003 0.0008 0.001 0.0008 0.0026 0.0014 0.003 0.0004 0.0056 0.0021 0.1367 0.0031
Golgi 0.0001 0 0 0 0.0027 0.0001 0.0012 0.0001 0.0002 0.0001 0.0002 0.0005 0.0025 0 0.0018 0 0.0496 0.0005
Lipid Particles 0.0169 0.0163 0.0228 0.0053 0.0176 0.0296 0.0335 0.054 0.0236 0.0602 0.0521 0.046 0.0111 0.0077 0.1194 0.0003 0.0129 0.0149
Mitochondria 0.0002 0 0 0 0.0005 0.0001 0.0045 0.0001 0.0014 0 0.0001 0.0001 0.0025 0.0001 0.0111 0.0001 0.0131 0.0009
None 0.0013 0 0 0 0.0012 0 0.0052 0 0.0014 0 0 0 0.0004 0 0.0002 0.0009 0.0017 0.0002
Nuclear Periphery 0.0052 0.0034 0.0024 0.0008 0.0023 0.006 0.0068 0.0036 0.006 0.0008 0.0018 0.0103 0.0163 0.0007 0.0012 0.0013 0.0168 0.0107
Nucleolus 0.0061 0.0033 0.0041 0.0004 0.0007 0.001 0.012 0.0017 0.0037 0.0023 0.0007 0.0021 0.0089 0.0326 0.049 0.0009 0.0011 0.0072
Nucleus 0.0019 0.0019 0.0009 0.0005 0.0006 0.0009 0.0046 0.0009 0.0009 0.0007 0.0009 0.0011 0.0094 0.0029 0.004 0.0018 0.0026 0.014
Peroxisomes 0.0003 0 0 0 0.0057 0 0.0063 0 0.0002 0 0 0 0.0002 0 0.0002 0 0.0034 0.0003
Punctate Nuclear 0.0007 0.0001 0 0 0.003 0 0.0038 0 0.0009 0 0 0 0.0002 0 0 0 0.0021 0.0006
Vacuole 0.5522 0.5562 0.5532 0.6575 0.5758 0.5636 0.5314 0.5255 0.5243 0.6089 0.5733 0.5538 0.5012 0.5412 0.5058 0.5465 0.2723 0.5165
Vacuole Periphery 0.0094 0.0067 0.0079 0.0167 0.0191 0.0144 0.0395 0.0206 0.0218 0.0254 0.0551 0.0514 0.0567 0.0663 0.0579 0.0675 0.2348 0.0415

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 380.3402 425.9352 499.0457 981.5486 579.8314 250.6349 576.1057 782.7645 620.2151 793.2768
Translational Efficiency 2.0213 2.7242 3.5306 0.9016 2.1763 4.0175 2.3477 1.8745 2.7553 2.0874

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
Localization
Cell Percentages ER (72%)
Cell Cycle Regulation No
Subcompartmental Group ER-4

Pmp2

Pmp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available