Standard name
Human Ortholog
Description P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0.07 0.06 0.09 0.07 0.07 – 0.09 0.06 0.09 0 0 0 0.19 – – 0 0 0 0 0 0
Bud 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0.09 0.07 0.08 0.07 0 0 – 0.06 0.06 0.08 0.12 0.16 0.15 0.44 – – 0 0 0 0 0 0
Cytoplasm 0.05 0.17 0 0.07 0.09 0.07 0.1 0.08 – 0.13 0.14 0.14 0.07 0.06 0.09 0.31 – – 0 0.09 0 0 0 0
Endoplasmic Reticulum 0.93 0.77 0.94 0.82 0.78 0.84 0.77 0.75 – 0.7 0.65 0.65 0.89 0.87 0.86 0 – – 0.94 0.69 0.94 0.87 0.9 0.9
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0.16 0.12 0.11 0 0 0.05 – 0 0 0 0.05 0 0 0.12 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.06 0.07 – 0.13 0.16 0.13 0 0 0 0.19 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.06 0.11 – 0.07 0.07 0.05 0 0 0 0 – – 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 24 21 30 32 25 28 36 – 38 18 36 7 5 4 3 – – 2 18 11 10 9 6
Bud 0 31 0 1 0 2 0 4 – 3 4 4 0 0 0 0 – – 1 9 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 1 3 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 9 12 54 30 44 19 14 18 – 26 19 32 42 39 61 7 – – 1 5 10 6 7 9
Cytoplasm 19 81 22 27 53 20 39 41 – 55 43 55 24 14 36 5 – – 9 63 0 7 5 4
Endoplasmic Reticulum 350 373 566 330 438 238 291 389 – 306 195 251 319 208 350 0 – – 364 464 574 371 397 457
Endosome 0 1 0 0 1 0 0 0 – 0 0 0 0 0 0 0 – – 0 4 0 0 0 0
Golgi 1 3 95 50 64 11 15 28 – 6 3 9 17 3 10 2 – – 0 10 3 10 5 6
Mitochondria 0 2 1 10 3 17 24 34 – 57 49 49 0 0 0 3 – – 0 13 4 7 6 5
Nucleus 0 2 0 4 1 0 0 1 – 1 0 0 1 1 1 0 – – 0 2 0 0 0 0
Nuclear Periphery 2 0 5 5 9 9 11 17 – 18 13 18 1 1 1 0 – – 0 6 1 1 0 1
Nucleolus 0 0 0 1 0 0 0 3 – 0 0 1 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 7 9 4 10 42 7 23 59 – 30 22 21 6 6 7 0 – – 3 57 0 3 2 7
Unique Cell Count 375 483 604 401 559 283 379 520 439 299 384 358 239 406 16 388 671 609 426 443 509
Labelled Cell Count 390 538 768 498 687 348 445 630 540 366 476 417 277 470 20 388 671 609 426 443 509


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.5 35.1 31.8 28.9 28.7 23.4 22.6 21.4 – 18.2 18.5 18.4 35.7 36.2 35.2 22.5 – – 37.3 38.3 41.4
Std Deviation (1e-4) 6.9 8.0 6.0 6.5 6.3 5.0 4.8 5.2 – 4.4 4.4 4.2 8.0 8.5 8.3 7.6 – – 8.5 8.7 9.1
Intensity Change (Log2) – – – -0.14 -0.15 -0.45 -0.49 -0.57 – -0.8 -0.78 -0.79 0.17 0.19 0.15 -0.5 – – 0.23 0.27 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.8 1.8 3.4 2.7 2.6 – 3.6 1.8 3.9 -1.4 -1.0 -2.5 0 – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Cell Periphery -0.8 -0.7 -1.1 -3.2 -3.7 – -1.8 -1.3 -0.3 1.4 3.1 3.0 0 – –
Cytoplasm 2.2 4.1 2.2 4.2 3.1 – 5.4 5.9 6.1 2.2 1.4 3.5 0 – –
Endoplasmic Reticulum -5.7 -7.6 -4.6 -7.7 -8.8 – -10.3 -11.1 -11.5 -2.5 -3.2 -4.0 -13.1 – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Golgi -1.4 -2.1 -5.1 -5.7 -5.5 – -7.7 -6.7 -6.7 -5.1 -5.9 -6.8 0 – –
Mitochondria 0 0 5.8 6.0 6.1 – 8.9 10.0 8.8 0 0 0 0 – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 1.2 0 2.5 2.9 – 3.6 3.6 3.9 0 0 0 0 – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Vacuole 2.4 6.0 0 5.0 7.8 – 5.5 5.7 4.7 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 33.5833 43.2308 40.6347 39.308 35.1728 39.8448 27.3893 44.3338 40.7802 37.4629 31.688 38.0267 37.3523 46.6191 44.7516 41.6891 46.1666 42.5679
Actin 0.0275 0.0014 0.0084 0.0018 0.0249 0.0015 0.014 0.0194 0.0004 0.0066 0.0012 0.0075 0.0637 0.0009 0.0022 0.0229 0.0001 0.0033
Bud 0.0011 0 0.0001 0.0001 0.0004 0.0002 0.0007 0.0005 0.0004 0 0 0 0.0005 0.0001 0.0003 0.0001 0 0
Bud Neck 0.006 0.0008 0.0009 0.0057 0.0063 0.0035 0.0054 0.0008 0.0008 0.0004 0.0006 0.0004 0.001 0.0005 0.0009 0.0009 0.0004 0.0011
Bud Periphery 0.002 0.0002 0.0003 0.0003 0.0008 0.0013 0.0009 0.0005 0.0007 0.0001 0.0002 0.0001 0.0012 0.0006 0.0003 0.0007 0.0001 0.0002
Bud Site 0.0071 0.0001 0.0006 0 0.0011 0.0001 0.0519 0.0136 0.0085 0.0007 0 0 0.0022 0.0014 0.0072 0.0002 0 0
Cell Periphery 0.0075 0.0281 0.0237 0.0137 0.0178 0.0066 0.0048 0.0694 0.019 0.0048 0.0028 0.0014 0.0098 0.0546 0.01 0.0176 0.0094 0.0044
Cytoplasm 0.0242 0.0255 0.0148 0.0243 0.0149 0.0351 0.0047 0.0048 0.0016 0.002 0.0029 0.0096 0.0027 0.0504 0.0165 0.0023 0.0009 0.0008
Cytoplasmic Foci 0.0242 0.0002 0.0129 0.0003 0.0158 0.003 0.0276 0.0062 0.0007 0.0177 0.0003 0.0004 0.0182 0.002 0.001 0.0002 0 0.0002
Eisosomes 0.0013 0.0024 0.0008 0.0017 0.0006 0.0001 0.0004 0.0006 0.0002 0.0001 0.0002 0.0001 0.0015 0.0013 0.0002 0.0043 0.0688 0.0005
Endoplasmic Reticulum 0.6216 0.8828 0.8799 0.8853 0.8041 0.881 0.5977 0.8348 0.9116 0.9143 0.8299 0.938 0.7092 0.8232 0.9344 0.8918 0.8745 0.9148
Endosome 0.0235 0.0009 0.0029 0.0016 0.0089 0.0025 0.0563 0.0018 0.0063 0.0074 0.0107 0.0012 0.0166 0.0014 0.0014 0.0015 0.0006 0.002
Golgi 0.008 0.0069 0.0016 0.0044 0.0072 0.0007 0.0196 0.0056 0.0017 0.0056 0.0574 0.0039 0.0093 0.0011 0.0012 0.0031 0.0063 0.0033
Lipid Particles 0.0188 0.0073 0.0083 0.0025 0.0299 0.0026 0.0293 0.0087 0.0058 0.0157 0.0031 0.0012 0.0329 0.0036 0.0006 0.002 0.0003 0.0018
Mitochondria 0.0233 0.0006 0.002 0.0002 0.023 0.0018 0.0119 0.001 0.0004 0.0003 0.0061 0.0005 0.0112 0.0002 0.0003 0.0069 0.0115 0.0021
None 0.0072 0.0002 0.0011 0.0001 0.0025 0.0009 0.0013 0.0001 0.0002 0.0005 0.0001 0 0.005 0.0001 0.0001 0.0008 0.0005 0.0003
Nuclear Periphery 0.1489 0.0295 0.0323 0.0506 0.0219 0.0431 0.139 0.0222 0.0241 0.0152 0.0696 0.0326 0.0838 0.0492 0.0179 0.0373 0.0216 0.0512
Nucleolus 0.0012 0.0001 0.0001 0 0.0006 0.0002 0.0017 0.0001 0.0043 0 0 0 0.0003 0 0.0001 0.0001 0 0.0015
Nucleus 0.0116 0.0019 0.0009 0.0006 0.0035 0.0022 0.0029 0.0003 0.0022 0.0002 0.0013 0.0005 0.002 0.0018 0.0014 0.0009 0.0005 0.0048
Peroxisomes 0.0072 0 0.0012 0 0.0041 0.0001 0.0156 0.0024 0.0001 0.0036 0 0.0001 0.0141 0 0.0002 0 0 0
Punctate Nuclear 0.0048 0 0.0005 0.0001 0.0023 0.0022 0.002 0.0004 0.0002 0.0012 0.0001 0.0001 0.0066 0 0.0001 0.0001 0 0.0001
Vacuole 0.0135 0.01 0.0057 0.004 0.0075 0.0079 0.0081 0.0063 0.009 0.0026 0.0065 0.0012 0.0041 0.0065 0.0028 0.0047 0.0035 0.0027
Vacuole Periphery 0.0095 0.001 0.0009 0.0026 0.0019 0.0037 0.0043 0.0005 0.002 0.0009 0.0071 0.0012 0.0042 0.0009 0.001 0.0017 0.001 0.0049

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 233.5055 199.5465 173.9546 171.9985 165.2969 203.4485 202.2397 182.4896 175.2498 202.8262
Translational Efficiency 0.7929 0.7585 0.7717 0.7134 0.7121 0.7913 0.718 0.5997 0.6607 0.6268

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-4

Spf1

Spf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available