Standard name
Human Ortholog
Description Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.1 0 0 0 0 0 0 – 0 0 0 0.19 0.06 0 0.17 0.12 0 0 0 0 0 0 0
Bud 0 0.05 0.06 0.07 0.06 0.06 0 0 – 0.07 0.09 0.09 0 0 0.07 0.06 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0 0 0.09 0 0 0 – 0 0 0 0.13 0.11 0.32 0.21 0.22 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.13 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.29 0 0.45 0.16 0.28 0 0 0 – 0 0 0 0.29 0.32 0.34 0.15 0.33 0.35 0.35 0.3 0.36 0.28 0.27 0.28
Golgi 0.17 0 0 0 0 0 0 0 – 0 0 0 0.13 0.11 0.06 0.28 0.16 0.35 0.45 0.28 0.4 0.41 0.44 0.38
Mitochondria 0.57 0.74 0.54 0.75 0.64 0.84 0.84 0.84 – 0.91 0.91 0.87 0.13 0.28 0.08 0.08 0.12 0.12 0.08 0.23 0.08 0.12 0.14 0.14
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.1 – 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0.15 0.08 0.1 0.09 0.13 0.1 – 0.07 0.07 0.09 0 0.07 0.08 0 0 0.12 0.05 0 0.1 0.1 0.05 0.05
SpindlePole 0.07 0 0.14 0.08 0.07 0.07 0.09 0.07 – 0.06 0.06 0.07 0.1 0.1 0.09 0.11 0.14 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0 0 0 0 0 0 – 0 0 0 0.16 0.1 0.08 0.15 0.1 0.24 0 0.08 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 20 4 0 0 1 0 2 – 1 3 3 12 6 3 9 10 0 3 4 4 5 1 5
Bud 7 11 20 12 13 23 18 11 – 30 35 34 0 0 8 3 5 0 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 2 10 4 2 – 5 6 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 0 0 2 0 3 0 – 0 3 2 1 1 0 1 1 0 0 0 0 0 0 0
Cytoplasm 4 24 15 7 20 15 11 11 – 6 2 6 8 12 35 11 18 2 1 4 0 0 1 1
Endoplasmic Reticulum 5 4 2 2 0 1 1 1 – 0 2 1 8 2 3 3 2 0 0 1 1 1 1 3
Endosome 62 9 161 28 65 17 11 9 – 9 7 9 18 34 37 8 27 6 92 75 148 44 56 63
Golgi 37 8 10 2 4 0 0 0 – 0 2 0 8 12 7 15 13 6 118 71 166 66 92 85
Mitochondria 124 153 192 131 147 328 337 297 – 367 352 326 8 29 9 4 10 2 20 57 33 19 28 31
Nucleus 2 1 1 0 1 1 1 3 – 2 0 2 1 0 0 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 2 1 – 6 1 2 0 1 0 0 3 0 0 1 0 0 0 0
Nucleolus 5 0 3 4 2 14 14 35 – 21 14 13 0 2 0 0 1 1 0 0 0 0 1 0
Peroxisomes 3 3 53 14 24 34 51 34 – 28 26 35 0 7 9 0 0 2 12 5 40 16 10 11
SpindlePole 16 2 50 14 17 26 35 23 – 24 25 25 6 10 10 6 11 3 2 2 4 0 1 0
Vac/Vac Membrane 24 27 14 1 5 6 1 2 – 1 2 3 10 11 9 8 8 4 9 20 6 3 10 13
Unique Cell Count 217 208 355 174 229 389 400 353 404 386 376 63 105 109 53 81 17 264 251 411 160 208 225
Labelled Cell Count 299 266 526 216 302 478 489 431 500 480 466 80 127 130 68 110 26 264 251 411 160 208 225


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.9 5.4 5.1 5.7 4.9 4.9 5.1 – 4.9 5.3 5.2 7.1 6.5 6.7 7.8 7.6 9.8 7.2 7.3 7.9
Std Deviation (1e-4) 0.8 1.2 1.1 1.0 1.8 1.1 1.3 1.0 – 1.1 1.0 1.1 1.1 1.1 1.4 1.7 1.6 2.6 1.6 1.6 2.3
Intensity Change (Log2) – – – -0.07 0.08 -0.15 -0.13 -0.09 – -0.13 -0.04 -0.05 0.39 0.27 0.31 0.52 0.5 0.86 0.41 0.43 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0.6 0 0 -0.7 -1.6 – 1.0 1.8 1.8 0 0 0.7 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 2.2 -0.3 -1.1 -0.8 – -2.3 -3.4 -2.1 0 2.8 8.2 0 5.5 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -6.6 -4.1 0 -13.9 -13.3 – -14.2 -14.1 -13.7 -2.5 -2.4 -2.1 -4.2 -2.0 -0.8
Golgi 0 -0.8 0 0 0 – 0 -2.5 0 0 3.6 0 0 0 0
Mitochondria 4.7 2.4 0 9.0 8.6 – 11.5 11.4 9.7 -6.1 -4.8 -8.4 -6.3 -6.8 -3.4
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.5 5.4 – 3.4 2.5 2.4 0 0 0 0 0 0
Peroxisomes -2.2 -1.6 0 -0.9 -2.1 – -3.6 -3.6 -2.3 -3.3 -2.2 -1.8 -3.0 -3.7 0
SpindlePole -2.0 -2.5 0 -2.3 -3.3 – -3.8 -3.4 -3.3 -1.0 -1.2 -1.3 -0.5 -0.1 0
Vacuole 0 -1.2 -2.0 -3.6 -3.0 – -3.7 -3.2 -2.8 0 2.6 1.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9718 4.4192 3.8104 3.8957 3.1394 4.1839 5.8215 7.339 7.023 5.4735 7.2754 7.1199 5.8773 7.3315 6.9827 7.1434 6.8568 7.1242
Actin 0.0062 0.0048 0.0092 0.0014 0.0251 0.0059 0.0173 0.0024 0.0222 0.0021 0.0024 0.012 0.0209 0.0047 0.0203 0.001 0.0004 0.0106
Bud 0.0003 0.0002 0.0002 0.0001 0.0024 0.0001 0.0002 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Bud Neck 0.0019 0.0003 0.0002 0.0002 0.0021 0.0002 0.0011 0.0002 0.0011 0.0013 0.0001 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.0005 0.0001 0.0002 0.0001 0.0035 0.0001 0.0002 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Bud Site 0.0083 0.0005 0.0005 0.0002 0.0082 0.0001 0.004 0.0041 0.0047 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0 0 0.0001
Cell Periphery 0.0004 0 0 0 0.0005 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0016 0.0023 0.0034 0.001 0.0027 0.0009 0.0021 0.0013 0.0015 0.0005 0.0006 0.0007 0.0012 0.0006 0.0006 0.0005 0.0003 0.0005
Cytoplasmic Foci 0.1141 0.1463 0.0974 0.1035 0.0999 0.0885 0.1365 0.1494 0.1315 0.1353 0.1211 0.106 0.1155 0.1232 0.1072 0.1026 0.0894 0.0859
Eisosomes 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0006 0.0004 0.0012 0.0002 0.0012 0.0002 0.0008 0.0002 0.0009 0.0001 0.0002 0.0002 0.0008 0.0001 0.0001 0.0001 0.0001 0.0002
Endosome 0.2505 0.2721 0.2765 0.2648 0.2574 0.155 0.1874 0.1805 0.1611 0.1237 0.1752 0.1247 0.1804 0.157 0.1386 0.2057 0.1137 0.112
Golgi 0.4445 0.5147 0.5391 0.5729 0.4055 0.657 0.5547 0.6225 0.5863 0.6569 0.6271 0.6567 0.5985 0.6739 0.6668 0.6694 0.6818 0.7141
Lipid Particles 0.0507 0.0124 0.0106 0.0207 0.0342 0.0269 0.0157 0.0031 0.012 0.0036 0.0241 0.0202 0.0176 0.0083 0.0059 0.0031 0.0725 0.0285
Mitochondria 0.0513 0.0206 0.0256 0.0061 0.0455 0.0269 0.016 0.009 0.0146 0.0046 0.0199 0.0198 0.019 0.0072 0.0214 0.0112 0.0029 0.014
None 0.0005 0.0001 0.0007 0 0.0023 0 0.0015 0.0001 0.002 0 0 0.0001 0.0003 0 0 0 0 0
Nuclear Periphery 0.0003 0.0001 0.0021 0.0001 0.0044 0 0.0005 0 0.0007 0 0.0001 0.0001 0.0004 0 0 0 0 0
Nucleolus 0.0005 0 0.0001 0 0.0198 0 0.0001 0 0.0001 0 0 0.0008 0 0 0 0 0 0
Nucleus 0.0003 0 0.001 0 0.0481 0 0.0002 0 0.0002 0 0 0.0006 0.0001 0 0 0 0 0
Peroxisomes 0.0622 0.0227 0.0275 0.0264 0.0189 0.0366 0.0589 0.0261 0.0579 0.0711 0.0271 0.0554 0.0426 0.0212 0.0376 0.0053 0.0377 0.0325
Punctate Nuclear 0.0006 0.0002 0.001 0.0001 0.004 0.0001 0.0008 0.0001 0.0013 0.0001 0.0001 0.0005 0.0007 0.0001 0.0001 0 0.0001 0.0001
Vacuole 0.0022 0.001 0.0014 0.0009 0.0089 0.0004 0.001 0.0003 0.0006 0.0001 0.0006 0.0006 0.0005 0.0016 0.0002 0.0002 0.0003 0.0004
Vacuole Periphery 0.0022 0.0013 0.0021 0.0013 0.0053 0.0009 0.0009 0.0005 0.0008 0.0003 0.0011 0.0009 0.0009 0.0016 0.0005 0.0007 0.0007 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.187 63.6583 39.4455 37.034 36.1698 53.5622 44.9559 41.4266 44.5345 42.6601
Translational Efficiency 0.7551 0.6807 0.7658 0.804 0.9286 0.5579 0.6831 0.7384 0.5729 0.5713

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
Localization
Cell Percentages Golgi (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Anp1

Anp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Anp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available