Standard name
Human Ortholog
Description Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.96 0.91 0.7 0.55 0.56 0.48 0.48 0.41 0.45 0.99 0.99 0.99 0.94 0.91 0.95 0.83 0.86 0.69 0.76 0.77 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.07 0.21 0.49 0.64 0.58 0.68 0.62 0.69 0.69 0 0 0 0 0 0 0.08 0.06 0.15 0.12 0.09 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.07 0.07 0.13 0.06 0.06 0 0 0 0 0.09 0.05 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 0 0 6 3 12 2 3 2
Bud 2 2 1 1 0 5 3 9 10 7 9 14 0 0 0 0 1 0 2 0 1 1 4 3
Bud Neck 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 4 1 5 6
Bud Site 0 0 0 0 0 1 0 0 2 2 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 0 2 0 1 1 2 1 1 4 2 4 0 2 1 0 1 3 1 1 1 2 1
Cytoplasm 508 325 311 252 255 273 270 301 210 246 174 216 321 353 430 49 30 35 456 290 233 153 209 216
Endoplasmic Reticulum 1 3 2 1 0 2 2 2 0 1 3 5 2 1 2 3 3 1 0 0 4 1 1 3
Endosome 1 0 0 2 1 3 0 1 4 1 4 2 1 2 0 1 0 0 13 6 6 1 1 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0
Mitochondria 4 3 15 19 59 192 311 309 297 316 292 332 0 0 0 0 0 0 43 20 50 23 24 29
Nucleus 0 0 0 1 0 3 4 11 6 6 6 9 1 1 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 3 1 3 5 4 2 0 1 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 1 1 3 0 3 2 5 2 7 4 9 0 0 0 0 0 0 5 1 4 1 2 1
Vac/Vac Membrane 10 1 4 2 8 13 23 35 32 69 24 28 0 1 2 0 3 2 6 3 5 9 10 12
Unique Cell Count 520 331 325 262 279 388 487 537 435 513 424 484 324 357 434 52 33 37 550 338 336 202 271 287
Labelled Cell Count 529 338 337 284 323 496 619 678 569 661 526 626 330 361 437 55 37 39 550 338 336 202 271 287


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.1 3.7 3.9 3.6 3.8 3.1 3.7 3.5 3.4 3.7 3.6 5.5 5.8 5.9 7.4 7.3 6.9 5.5 5.8 5.7
Std Deviation (1e-4) 0.6 0.7 1.2 1.9 1.1 1.3 1.0 1.6 1.1 1.1 1.4 1.1 0.9 1.4 1.4 1.4 1.0 1.1 1.5 1.8 1.6
Intensity Change (Log2) – – – 0.11 -0.01 0.06 -0.23 0.01 -0.07 -0.09 0.02 -0.03 0.6 0.65 0.7 1.01 0.99 0.92 0.58 0.66 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4531 2.8928 2.3507 1.5208 1.4527 2.4332 3.5425 4.1328 3.6412 3.0271 3.6484 3.818 2.7456 2.3082 1.7589 1.8047 2.9184 2.0086
Actin 0.0011 0.0008 0.0015 0.0006 0.0007 0.0012 0.0034 0.0007 0.0033 0.001 0.0014 0.0019 0.1069 0.0003 0.0294 0.0177 0.0939 0.025
Bud 0.0039 0.0046 0.0016 0.0022 0.0009 0.0035 0.0015 0.0028 0.0039 0.0027 0.0011 0.0006 0.0007 0.0008 0.0008 0.0006 0.0008 0.0003
Bud Neck 0.0008 0.0005 0.001 0.0006 0.0006 0.0047 0.0004 0.0003 0.0008 0.0005 0.0008 0.0011 0.0015 0.0001 0.0161 0.0004 0.0025 0.0013
Bud Periphery 0.0017 0.0032 0.0015 0.0051 0.0007 0.0018 0.0011 0.0024 0.0057 0.0025 0.0011 0.0005 0.0009 0.0003 0.0007 0.0008 0.0017 0.0006
Bud Site 0.003 0.0135 0.0048 0.0015 0.0003 0.0009 0.0027 0.0032 0.0077 0.0008 0.0007 0.0002 0.0026 0.0008 0.0152 0.0002 0.0017 0.0003
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0 0.0004 0.0001 0.0003 0.0001 0.0003 0.0001
Cytoplasm 0.5669 0.6106 0.5456 0.4302 0.4958 0.6102 0.6099 0.6657 0.5439 0.5697 0.682 0.6111 0.4509 0.6631 0.5177 0.5836 0.3916 0.6718
Cytoplasmic Foci 0.0213 0.0179 0.0174 0.0188 0.0182 0.0218 0.0167 0.0088 0.0146 0.0221 0.018 0.0165 0.0167 0.0024 0.0088 0.0224 0.0258 0.0076
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0004 0 0.0001 0.0001 0.001 0.0002
Endoplasmic Reticulum 0.0055 0.0031 0.0029 0.0043 0.0025 0.0037 0.0046 0.0028 0.0048 0.0026 0.0033 0.0022 0.0063 0.0022 0.004 0.0061 0.0067 0.0032
Endosome 0.0107 0.006 0.0069 0.006 0.008 0.0113 0.0063 0.0046 0.009 0.0173 0.0106 0.0068 0.0182 0.0006 0.0068 0.0112 0.0528 0.0067
Golgi 0.0005 0.0005 0.0005 0.0004 0.0003 0.0007 0.0006 0.0003 0.0012 0.0007 0.0009 0.0005 0.017 0.0001 0.0068 0.002 0.0387 0.0019
Lipid Particles 0.0006 0.0003 0.0004 0.0003 0.0001 0.0003 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0113 0.0001 0.0048 0.0067 0.034 0.001
Mitochondria 0.0007 0.0008 0.001 0.0012 0.0009 0.0016 0.0004 0.0009 0.0068 0.0022 0.0008 0.0006 0.0047 0.0001 0.003 0.0014 0.0207 0.0005
None 0.3578 0.3062 0.3913 0.498 0.4534 0.311 0.3435 0.2966 0.3779 0.3636 0.2475 0.3471 0.2731 0.3256 0.3727 0.3062 0.2484 0.2576
Nuclear Periphery 0.0027 0.0022 0.002 0.0024 0.0021 0.0026 0.0014 0.0012 0.0024 0.0022 0.0015 0.0011 0.0123 0.0006 0.0024 0.0044 0.0427 0.0113
Nucleolus 0.0006 0.0006 0.0004 0.0005 0.0004 0.0006 0.0001 0.0002 0.0005 0.0003 0.0004 0.0001 0.0006 0 0.0003 0.0001 0.0019 0.0003
Nucleus 0.0124 0.0202 0.0118 0.0118 0.0068 0.0144 0.0032 0.0061 0.0077 0.0046 0.0135 0.0054 0.0404 0.0018 0.0016 0.0037 0.0062 0.004
Peroxisomes 0.0006 0.0013 0.0007 0.0007 0.0011 0.0008 0.0003 0.0003 0.0007 0.0012 0.0004 0.0009 0.0097 0 0.0033 0.0229 0.0054 0.0005
Punctate Nuclear 0.0036 0.0049 0.006 0.0115 0.003 0.0055 0.0011 0.0009 0.004 0.0023 0.013 0.0017 0.0027 0.0003 0.0036 0.0044 0.0164 0.0043
Vacuole 0.0048 0.0022 0.002 0.0032 0.0032 0.0028 0.0021 0.0019 0.0035 0.0025 0.0024 0.0014 0.0198 0.0006 0.0013 0.0039 0.0033 0.0011
Vacuole Periphery 0.0007 0.0003 0.0003 0.0005 0.0006 0.0006 0.0003 0.0002 0.0007 0.0007 0.0004 0.0003 0.0031 0.0001 0.0003 0.0012 0.0034 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1416 2230 376 154 1813 1786 1393 142 3229 4016 1769 296

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.21 729.25 1284.69 886.28 685.60 679.39 793.66 832.53 659.56 707.08 898.03 860.49
Standard Deviation 80.59 87.03 371.49 136.54 86.46 83.43 105.85 107.29 88.96 88.96 280.20 126.27
Intensity Change Log 2 — 0.219767 1.036702 0.501116 — -0.013127 0.211154 0.280135 — 0.102744 0.663880 0.389851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000499 0.001572 0.001436 0.000095 0.000817 0.000113 0.000151 0.000079 0.000640 0.000423 0.000819
Bud Neck 0.005927 0.045767 0.007249 0.004749 0.016514 0.053653 0.003960 0.000979 0.011871 0.049274 0.004659 0.002940
Bud Site 0.002966 0.019467 0.023766 0.023905 0.004667 0.025921 0.006260 0.001868 0.003921 0.022337 0.009981 0.013333
Cell Periphery 0.000115 0.000195 0.000288 0.000261 0.000118 0.000223 0.000100 0.000289 0.000117 0.000208 0.000140 0.000274
Cytoplasm 0.588747* 0.418346* 0.469752* 0.351690* 0.501552* 0.300264* 0.483959* 0.487631* 0.539789* 0.365833* 0.480940* 0.416905*
Cytoplasmic Foci 0.130897 0.221340* 0.030202 0.033976 0.213829* 0.328750* 0.009556 0.115359 0.177461 0.269107* 0.013944 0.073018
Eisosomes 0.000072 0.000120 0.000042 0.000102 0.000133 0.000323 0.000024 0.000126 0.000106 0.000211 0.000028 0.000114
Endoplasmic Reticulum 0.000395 0.000570 0.004364 0.004970 0.000726 0.000760 0.001258 0.001381 0.000581 0.000654 0.001918 0.003248
Endosome 0.004621 0.017959 0.010538 0.014858 0.013729 0.035453 0.001406 0.012264 0.009735 0.025739 0.003347 0.013613
Golgi 0.000643 0.005652 0.002023 0.001706 0.002945 0.017849 0.000115 0.000158 0.001935 0.011076 0.000520 0.000963
Lipid Particles 0.004318 0.005690 0.003162 0.004099 0.009014 0.011205 0.000732 0.004958 0.006955 0.008142 0.001248 0.004511
Mitochondria 0.003383 0.016531 0.000501 0.000661 0.004344 0.042446 0.000505 0.000163 0.003923 0.028056 0.000504 0.000422
Mitotic Spindle 0.000663 0.007667 0.019776 0.006254 0.001903 0.009982 0.003550 0.000121 0.001359 0.008697 0.006999 0.003312
None 0.012280 0.003412 0.006063 0.002579 0.004556 0.002142 0.005847 0.004489 0.007943 0.002847 0.005893 0.003495
Nuclear Periphery 0.000312 0.000411 0.001333 0.001750 0.000481 0.000284 0.001153 0.000777 0.000407 0.000355 0.001191 0.001283
Nuclear Periphery Foci 0.000318 0.000228 0.007860 0.004246 0.000342 0.000272 0.000653 0.003093 0.000331 0.000247 0.002185 0.003693
Nucleolus 0.001429 0.001426 0.000695 0.000986 0.001196 0.002186 0.000396 0.001071 0.001298 0.001764 0.000460 0.001027
Nucleus 0.215473* 0.137312* 0.274881* 0.343858* 0.167064* 0.072968 0.442333* 0.261604* 0.188292* 0.108697 0.406741* 0.304399*
Peroxisomes 0.001658 0.016847 0.001320 0.002075 0.004832 0.036720 0.000569 0.000685 0.003440 0.025685 0.000728 0.001408
Vacuole 0.025057 0.078680 0.133148 0.193938 0.050893 0.054331 0.037378 0.102514 0.039563 0.067851 0.057734 0.150079
Vacuole Periphery 0.000666 0.001883 0.001467 0.001902 0.001068 0.003452 0.000136 0.000319 0.000892 0.002581 0.000419 0.001142

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.63 -6.03 -2.17 -0.93 2.05 -4.22 -4.15 -2.23 3.55 -0.82 -6.03 -6.66 -2.38 0.53 -0.14
Bud Neck -18.22 -2.82 2.26 18.79 4.53 -12.69 8.14 11.68 20.02 4.68 -20.49 6.59 10.48 27.53 4.15
Bud Site -10.78 -5.36 -2.16 1.58 1.50 -11.01 -0.52 3.11 12.73 2.59 -15.23 -3.85 -1.56 5.90 0.42
Cell Periphery -2.58 -8.38 -3.05 0.16 4.84 -6.97 2.06 -7.40 -2.79 -7.98 -4.96 -4.05 -7.21 -1.45 -4.50
Cytoplasm 18.81 12.98 12.35 4.64 3.55 23.03 7.64 1.57 -8.56 -2.12 27.23 14.72 9.61 -1.61 1.98
Cytoplasmic Foci -14.18 21.93 18.10 31.78 -0.56 -15.43 42.31 9.43 19.41 -10.95 -18.18 48.06 16.74 29.84 -10.06
Eisosomes -4.01 5.47 -3.29 1.31 -6.13 -2.92 15.19 -0.20 2.86 -9.33 -3.48 16.97 -1.44 3.05 -11.72
Endoplasmic Reticulum -2.98 -10.63 -7.97 -7.69 -1.02 -0.31 -9.21 -3.89 -3.78 1.46 -1.04 -15.00 -8.36 -8.19 -2.58
Endosome -11.85 -3.69 -1.78 3.67 -0.14 -11.21 12.62 0.21 9.42 -7.21 -14.45 10.47 -0.81 8.62 -5.49
Golgi -11.53 -2.16 -0.10 7.27 1.16 -10.93 5.94 5.79 13.86 -1.75 -13.42 5.47 3.87 15.63 -0.23
Lipid Particles -2.04 1.07 1.36 3.51 0.42 -2.29 11.08 3.49 6.10 -6.76 -2.00 11.60 4.11 6.64 -7.26
Mitochondria -8.84 3.67 3.80 12.77 1.06 -14.14 6.75 7.42 16.06 7.97 -16.55 7.27 7.76 20.12 3.46
Mitotic Spindle -5.36 -5.85 -2.07 1.54 4.05 -4.95 -1.73 1.20 5.55 2.65 -7.01 -5.35 -1.17 4.40 3.18
None 6.36 5.44 7.38 2.06 3.16 3.86 -0.84 0.32 -1.87 0.84 7.10 4.16 5.63 -0.53 2.98
Nuclear Periphery -2.08 -15.57 -11.05 -10.21 -2.01 3.80 -17.03 -5.00 -7.44 7.29 1.36 -23.41 -11.24 -11.67 1.10
Nuclear Periphery Foci 2.27 -9.58 -7.70 -7.86 4.05 1.07 -3.86 -5.50 -5.62 -4.45 2.13 -9.68 -9.28 -9.49 -3.35
Nucleolus -0.17 4.51 3.70 4.79 -0.13 -2.62 7.43 0.62 2.78 -4.31 -2.49 8.45 3.33 4.63 -3.54
Nucleus 11.94 -5.29 -2.94 -6.60 0.17 18.95 -34.73 -4.78 -10.17 11.28 19.34 -31.82 -5.50 -11.22 10.43
Peroxisomes -15.87 1.74 1.02 14.34 0.06 -16.65 8.54 8.92 19.32 -1.21 -21.68 8.42 6.35 23.94 -0.94
Vacuole -19.02 -15.10 -12.90 -10.53 -4.76 -1.22 -4.56 -5.54 -5.27 -4.04 -13.47 -13.74 -12.77 -10.81 -8.59
Vacuole Periphery -7.45 -1.19 -0.25 1.92 0.25 -6.30 9.45 7.16 8.50 -5.81 -8.77 6.93 1.09 6.01 -0.95
Standard name
Human Ortholog
Description Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cyc7

Cyc7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyc7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available