Standard name
Human Ortholog
Description Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.05 0 0.05 0 0 0 0 0 0 0 0.07 0.09 0 0.17 0.16 0.21 0.08 0.05 0.09 0.07 0.06 0.14
Bud 0 0 0.06 0.05 0 0.05 0 0 0.05 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.06 0 0.05 0.07 0.08 0.05 0 0 0 0 0 0.15 0.2 0.15 0.07 0.14 0.12 0 0 0 0 0 0.05
Cytoplasm 0.27 0.54 0.79 0.74 0.8 0.76 0.76 0.75 0.67 0.64 0.51 0.57 0.75 0.8 0.71 0.66 0.64 0 0.19 0.1 0.12 0.16 0.1
Endoplasmic Reticulum 0.66 0.4 0.09 0.09 0 0.07 0 0 0 0 0.07 0.35 0.13 0.09 0.05 0.05 0 0.71 0.53 0.5 0.56 0.44 0.47
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.08 0.17 0.07 0.21 0.26 0.29 0.44 0.48 0.58 0 0 0.05 0 0 0 0.05 0.08 0.16 0.11 0.15 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 5 8 12 4 4 1 2 2 1 1 14 24 11 55 37 53 9 6 26 13 13 43
Bud 2 0 15 11 16 19 23 23 28 28 14 1 6 18 7 2 10 0 0 1 2 2 1
Bud Neck 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 2 0 2 3 7 6 6 7
Bud Site 0 0 1 0 1 2 2 0 0 1 1 0 0 0 0 0 0
Cell Periphery 5 3 11 17 31 20 22 14 10 16 22 30 55 44 21 33 29 2 2 9 2 4 16
Cytoplasm 24 57 190 175 301 316 416 460 352 265 263 112 209 231 227 153 160 3 23 27 23 39 32
Endoplasmic Reticulum 59 42 22 21 17 30 18 10 11 2 38 68 37 26 16 11 8 82 65 142 116 107 150
Endosome 2 0 0 1 10 4 4 9 2 0 4 1 2 0 7 6 4 1 1 5 3 5 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 3 1 2 2
Mitochondria 1 10 19 40 28 86 143 176 229 200 300 1 2 15 12 9 8 6 10 45 22 37 37
Nucleus 1 0 0 1 1 1 0 2 0 0 0 0 1 1 3 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 1 0 1 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 0 2 4 3 1 1 0 0 0 3 5 2 0 0 0 0 0 1
Vac/Vac Membrane 1 0 6 0 17 3 17 13 2 5 10 0 0 2 5 6 8 2 5 10 9 20 12
Unique Cell Count 90 105 240 238 378 414 550 610 522 416 513 195 277 290 318 233 250 116 123 285 206 246 319
Labelled Cell Count 104 117 272 279 432 487 649 715 640 519 655 227 336 348 359 266 283 116 123 285 206 246 319


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.7 4.8 4.8 4.9 4.5 4.3 4.2 3.5 3.7 3.6 6.4 6.2 5.4 7.2 7.9 8.0 6.3 5.6 6.3
Std Deviation (1e-4) 0.9 0.9 0.8 1.1 1.3 1.1 1.2 0.9 0.8 0.9 1.0 1.2 1.0 0.9 1.0 1.2 1.2 1.0 1.1 1.2
Intensity Change (Log2) 0.0 0.03 -0.1 -0.17 -0.19 -0.45 -0.39 -0.43 0.41 0.39 0.18 0.6 0.73 0.74 0.39 0.24 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 1.8 2.5 0.3 5.2 4.6 6.0
Bud 0 -1.1 0 0 0 0 0 0 -3.2 -2.3 0 -2.4 -3.1 -1.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.7 0 0 0 0 0 0 3.8 5.2 4.0 1.0 3.6 2.8
Cytoplasm -1.5 0.1 -0.8 -1.1 -1.2 -3.3 -4.1 -7.3 -4.9 -1.0 0.1 -2.1 -3.3 -3.7
Endoplasmic Reticulum 0 -2.3 0 0 0 0 0 0 6.6 1.5 -0.1 -1.9 -1.9 -2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.2 0 0 0 0 0 0 -3.7 -4.1 -1.3 -2.1 -1.9 -2.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0 0.5 -0.3 0 0 -0.5 0 0 0 0 0.1 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6825 2.8354 2.5108 2.6144 2.3991 2.9515 3.2594 3.5417 3.165 2.8452 2.8256 3.209 3.4865 3.3717 3.412 3.2405 3.4906 3.3857
Actin 0.0133 0.0098 0.0246 0.0081 0.0044 0.0058 0.022 0.0036 0.0148 0.0183 0.0071 0.0038 0.015 0.0038 0.0184 0.0353 0.0159 0.0099
Bud 0.0001 0.0003 0.0007 0.0002 0.0004 0.0002 0.0007 0.0004 0.0013 0.0015 0.0008 0.0002 0.0002 0.0002 0.0005 0.0006 0.0003 0.0002
Bud Neck 0.0027 0.0016 0.0033 0.0031 0.0075 0.0291 0.0027 0.0011 0.0058 0.0125 0.0108 0.012 0.0019 0.0009 0.0028 0.0093 0.004 0.0151
Bud Periphery 0.0005 0.0005 0.0027 0.0005 0.0063 0.0008 0.0016 0.0008 0.0033 0.0059 0.0025 0.0006 0.0006 0.0004 0.0024 0.0019 0.001 0.0005
Bud Site 0.0022 0.0254 0.0057 0.0026 0.001 0.0005 0.0042 0.0182 0.0094 0.0067 0.0019 0.0003 0.0022 0.0279 0.0077 0.0027 0.0015 0.0003
Cell Periphery 0.0183 0.0117 0.0138 0.0096 0.0205 0.0078 0.0096 0.0118 0.0121 0.0069 0.0075 0.0026 0.0152 0.0079 0.0151 0.0115 0.005 0.0054
Cytoplasm 0.347 0.3714 0.4176 0.4145 0.4504 0.4874 0.3341 0.4445 0.3976 0.3263 0.3856 0.4378 0.3844 0.3955 0.4303 0.3924 0.4118 0.5489
Cytoplasmic Foci 0.0251 0.027 0.028 0.0409 0.0363 0.0194 0.0385 0.029 0.0337 0.0916 0.0318 0.0152 0.017 0.0262 0.0276 0.0187 0.0324 0.0145
Eisosomes 0.0015 0.0004 0.0006 0.0004 0.0004 0.0004 0.0008 0.0004 0.0004 0.0007 0.0002 0.0001 0.0017 0.0006 0.0012 0.0017 0.0011 0.0007
Endoplasmic Reticulum 0.3952 0.408 0.3528 0.2974 0.3709 0.3254 0.296 0.2973 0.302 0.2457 0.3269 0.3876 0.3829 0.2997 0.2831 0.3087 0.2214 0.2655
Endosome 0.0281 0.0309 0.0312 0.0397 0.026 0.0239 0.0553 0.0289 0.0437 0.049 0.0511 0.0198 0.0153 0.0539 0.0264 0.0232 0.0246 0.0087
Golgi 0.0092 0.0134 0.0098 0.0081 0.0037 0.0057 0.0153 0.0063 0.0144 0.0201 0.0137 0.0046 0.0061 0.0122 0.0099 0.0148 0.0121 0.0022
Lipid Particles 0.0597 0.0435 0.044 0.0733 0.0345 0.0204 0.0659 0.0381 0.0361 0.0534 0.0385 0.0185 0.0492 0.0461 0.0436 0.043 0.0291 0.0136
Mitochondria 0.0024 0.0012 0.0012 0.0007 0.0009 0.0014 0.0108 0.0023 0.0035 0.0115 0.0272 0.0018 0.0009 0.0022 0.0019 0.0083 0.002 0.0004
None 0.0675 0.0296 0.0435 0.0861 0.0205 0.0585 0.0813 0.0918 0.091 0.0847 0.0321 0.0828 0.085 0.1063 0.1115 0.1101 0.2202 0.1064
Nuclear Periphery 0.0112 0.0087 0.0049 0.0039 0.0056 0.0041 0.0154 0.0047 0.0075 0.0084 0.0115 0.0049 0.008 0.0062 0.0044 0.004 0.0051 0.0024
Nucleolus 0.0004 0.0003 0.0002 0.0001 0.0001 0.0002 0.0014 0.0004 0.0006 0.0012 0.0043 0.0001 0.0005 0.0003 0.0005 0.0003 0.001 0.0001
Nucleus 0.003 0.0024 0.0019 0.0013 0.0021 0.0019 0.0066 0.0034 0.003 0.0049 0.011 0.0015 0.004 0.0013 0.0015 0.0013 0.0031 0.001
Peroxisomes 0.0036 0.0007 0.0023 0.0008 0.0005 0.0004 0.0168 0.0045 0.0048 0.0278 0.009 0.0003 0.0008 0.0007 0.0023 0.0008 0.001 0.0003
Punctate Nuclear 0.0019 0.0017 0.0015 0.0015 0.0012 0.0015 0.0093 0.0033 0.0051 0.0073 0.0127 0.0008 0.0014 0.0017 0.002 0.0016 0.0026 0.0007
Vacuole 0.0064 0.0109 0.0092 0.0069 0.0062 0.0049 0.0097 0.0087 0.0092 0.014 0.0101 0.0043 0.0072 0.0055 0.0065 0.0093 0.0046 0.0029
Vacuole Periphery 0.0007 0.0006 0.0005 0.0004 0.0004 0.0005 0.0022 0.0006 0.0008 0.0017 0.0037 0.0005 0.0004 0.0006 0.0005 0.0006 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.6361 19.9268 26.0039 28.0198 19.4336 26.5039 31.3509 32.3551 26.3739 34.6131
Translational Efficiency 0.4684 0.5904 0.4468 0.3166 0.4281 0.4759 0.3881 0.3962 0.4917 0.3803

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
624 672 181 1050 1398 1956 1336 145 2022 2628 1517 1195

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.74 784.43 1044.98 903.14 692.53 811.85 964.37 976.16 693.21 804.84 973.99 912.00
Standard Deviation 108.06 104.66 115.34 107.79 92.14 99.80 105.65 121.17 97.34 101.77 110.00 112.07
Intensity Change Log 2 0.175172 0.588930 0.378476 0.229337 0.477710 0.495241 0.202465 0.534480 0.437947

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001152 0.005822 0.003652 0.014993 0.001977 0.002389 0.004944 0.055867 0.001722 0.003267 0.004790 0.019952
Bud Neck 0.015416 0.022387 0.004665 0.022900 0.009417 0.013904 0.013858 0.023604 0.011268 0.016073 0.012762 0.022986
Bud Site 0.006551 0.021781 0.013799 0.053611 0.006613 0.014586 0.024527 0.081735 0.006594 0.016426 0.023247 0.057023
Cell Periphery 0.004542 0.003370 0.002068 0.002484 0.003176 0.002985 0.003196 0.012634 0.003597 0.003084 0.003062 0.003716
Cytoplasm 0.262938* 0.234326* 0.168392* 0.308788* 0.289420* 0.338810* 0.327319* 0.250911* 0.281247* 0.312092* 0.308357* 0.301765*
Cytoplasmic Foci 0.108416 0.194439* 0.118200 0.139458 0.149506 0.127871 0.089007 0.092600 0.136825 0.144893 0.092491 0.133772
Eisosomes 0.005939 0.005231 0.000679 0.004726 0.005584 0.005251 0.002299 0.011852 0.005693 0.005246 0.002106 0.005590
Endoplasmic Reticulum 0.240502* 0.174529* 0.199831* 0.049183 0.197337* 0.191157* 0.227140* 0.132523* 0.210658* 0.186905* 0.223881* 0.059295
Endosome 0.026337 0.044813 0.051194 0.024658 0.018817 0.024698 0.016580 0.012979 0.021138 0.029841 0.020710 0.023240
Golgi 0.061093 0.077380 0.004372 0.013250 0.083525 0.065533 0.008598 0.010724 0.076603 0.068562 0.008094 0.012943
Lipid Particles 0.162996* 0.094958 0.081716 0.056438 0.123449* 0.104327* 0.069757 0.053042 0.135653* 0.101931* 0.071184 0.056026
Mitochondria 0.025229 0.024845 0.000146 0.002838 0.036274 0.022955 0.000929 0.000301 0.032865 0.023438 0.000836 0.002530
Mitotic Spindle 0.000103 0.001583 0.000554 0.007276 0.000566 0.001942 0.000673 0.000762 0.000423 0.001850 0.000659 0.006486
None 0.004131 0.003289 0.000113 0.000623 0.006631 0.007429 0.000696 0.000211 0.005860 0.006370 0.000626 0.000573
Nuclear Periphery 0.002212 0.001186 0.004752 0.000867 0.001162 0.000532 0.002895 0.000366 0.001486 0.000699 0.003117 0.000807
Nuclear Periphery Foci 0.002902 0.002719 0.092756 0.009442 0.001831 0.001906 0.031810 0.016472 0.002162 0.002114 0.039082 0.010295
Nucleolus 0.000553 0.000746 0.000262 0.000513 0.000875 0.000641 0.000249 0.000138 0.000775 0.000668 0.000251 0.000467
Nucleus 0.016380 0.018095 0.029480 0.054482 0.021290 0.026497 0.085380 0.042541 0.019775 0.024348 0.078710 0.053033
Peroxisomes 0.020430 0.019078 0.002203 0.011707 0.021254 0.016499 0.004335 0.007704 0.021000 0.017158 0.004081 0.011222
Vacuole 0.025097 0.040900 0.220529* 0.219909* 0.016107 0.025520 0.085347 0.192545 0.018881 0.029453 0.101477 0.216589*
Vacuole Periphery 0.007080 0.008524 0.000636 0.001854 0.005190 0.004571 0.000457 0.000491 0.005773 0.005582 0.000479 0.001689

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.63 -2.45 -7.53 -4.16 -5.22 -1.04 -4.83 -4.81 -4.77 -4.52 -3.97 -5.67 -8.49 -7.62 -6.75
Bud Neck -1.93 4.36 -2.02 0.31 -8.33 -3.19 -2.34 -3.06 -1.98 -1.96 -3.38 -0.82 -5.21 -2.78 -4.17
Bud Site -5.60 -2.09 -10.43 -6.24 -6.96 -5.99 -7.29 -6.32 -5.58 -4.66 -8.06 -7.55 -12.28 -9.51 -7.17
Cell Periphery 1.95 4.54 4.35 2.44 -0.69 0.51 0.07 -3.26 -3.35 -3.28 1.63 1.65 0.43 -0.78 -0.88
Cytoplasm 2.58 5.93 -3.46 -6.34 -8.60 -6.68 -4.18 2.44 5.25 4.37 -4.89 -3.32 -1.67 2.04 1.26
Cytoplasmic Foci -10.29 -1.02 -4.10 7.16 -1.94 4.25 11.91 6.04 3.89 -0.24 -1.78 10.19 1.08 2.63 -7.53
Eisosomes 0.71 6.25 1.60 1.09 -6.03 0.56 6.49 -2.20 -2.37 -3.69 0.87 8.08 0.85 0.25 -4.90
Endoplasmic Reticulum 4.39 2.25 16.13 12.14 8.38 0.71 -2.49 4.31 4.06 5.63 3.15 -0.94 22.19 21.33 20.43
Endosome -5.63 -5.69 1.22 6.90 6.58 -4.19 1.59 3.02 5.82 1.98 -6.36 0.32 -0.53 4.46 -0.76
Golgi -3.19 16.74 13.69 16.20 -4.88 5.40 27.43 19.70 16.95 -0.45 2.93 31.38 26.29 26.16 -2.74
Lipid Particles 7.95 8.43 14.06 7.15 3.60 3.90 10.94 8.71 6.77 2.45 7.82 14.54 18.06 12.63 4.45
Mitochondria 0.12 9.88 8.58 8.81 -3.32 5.48 16.26 16.56 20.85 5.37 4.77 18.85 16.85 17.59 -2.27
Mitotic Spindle -2.00 -2.11 -4.36 -2.93 -3.87 -1.86 -0.36 -0.77 1.20 -0.57 -2.53 -1.06 -4.17 -2.96 -3.98
None 0.37 3.44 3.01 2.29 -2.72 -0.53 6.12 6.64 7.21 3.25 -0.47 6.84 6.89 7.14 0.46
Nuclear Periphery 1.31 -2.04 1.91 0.73 3.91 3.04 -5.49 3.67 2.02 10.07 2.86 -4.58 2.44 -0.96 8.82
Nuclear Periphery Foci 0.24 -11.00 -7.52 -8.28 10.13 -0.33 -17.26 -6.51 -6.49 5.67 0.16 -19.64 -10.93 -11.21 14.27
Nucleolus -1.56 3.83 0.48 1.61 -1.49 1.29 2.97 3.44 7.92 2.89 -0.85 3.50 1.91 1.95 -1.52
Nucleus -1.10 -3.40 -13.72 -10.40 -5.56 -3.49 -19.35 -4.71 -3.62 7.62 -3.82 -19.97 -13.70 -11.47 7.16
Peroxisomes 0.60 12.18 5.09 4.64 -7.94 3.68 16.10 9.57 6.90 -2.67 3.51 19.38 8.12 5.46 -6.62
Vacuole -4.22 -14.51 -28.05 -25.18 -1.07 -6.08 -21.29 -11.11 -10.61 -7.07 -6.92 -23.83 -31.67 -30.02 -17.21
Vacuole Periphery -1.26 8.43 5.62 6.74 -1.78 1.02 10.14 9.22 9.08 0.02 0.37 13.20 6.53 6.42 -2.03
Standard name
Human Ortholog
Description Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
Localization
Cell Percentages ER (93%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gta1

Gta1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gta1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available