Standard name
Human Ortholog
Description Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.06 0 0.05 0.09 – 0.07 0.09 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.93 0.86 0.88 0.8 0.77 – 0.77 0.77 0.74 0.73 0.96 0.98 0.99 0.95 0.92 0.96 0.94 0.95 0.9 0.89 0.92 0.91
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.05
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.06 – 0.16 0.18 0.27 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.14 0.09 0.15 0.14 – 0.13 0.05 0.08 0.07 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 3 0 5 – 6 6 4 3 0 0 0 0 0 0 0 0 4 0 1 0
Bud 0 0 0 0 0 2 0 – 7 0 3 5 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 4 16 17 7 11 37 – 26 40 30 35 2 11 9 5 4 3 0 0 0 0 0 0
Cytoplasm 304 237 205 243 214 170 313 – 299 342 265 325 149 350 317 129 138 212 292 227 212 162 266 248
Endoplasmic Reticulum 6 5 10 3 4 1 2 – 3 15 3 11 7 9 4 4 2 10 10 3 8 8 16 12
Endosome 0 0 0 0 0 1 3 – 1 6 4 3 0 0 0 0 0 0 1 0 1 1 0 0
Golgi 0 0 1 1 8 1 11 – 1 0 1 0 0 0 0 0 2 0 0 0 1 0 0 2
Mitochondria 0 0 1 4 2 28 25 – 62 80 97 103 0 0 0 2 4 3 1 0 1 0 0 1
Nucleus 0 0 0 1 0 3 3 – 4 1 3 1 0 1 0 1 1 3 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 2 1 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 7 39 21 31 58 – 49 20 28 33 0 0 1 2 13 4 2 2 0 3 1 0
Unique Cell Count 310 240 221 282 242 212 406 389 444 358 443 156 357 321 136 150 220 312 239 236 182 290 273
Labelled Cell Count 317 246 240 310 259 250 459 459 510 438 520 159 371 331 143 164 236 312 239 236 182 290 273


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.1 18.3 17.2 17.3 15.8 10.7 11.6 – 9.7 9.1 8.7 8.5 21.1 23.5 23.5 21.0 19.7 21.4 16.9 18.5 19.6
Std Deviation (1e-4) 3.5 3.6 3.5 3.2 2.6 3.8 2.4 – 2.4 1.7 2.4 1.6 5.5 4.9 5.0 4.9 6.0 5.8 3.0 3.7 4.3
Intensity Change (Log2) – – – 0.0 -0.12 -0.69 -0.57 – -0.83 -0.92 -0.98 -1.03 0.29 0.45 0.45 0.28 0.19 0.31 -0.03 0.1 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -2.2 -0.9 0.8 – -0.3 0.8 0.5 0.3 -2.7 -2.3 -2.4 -1.4 -1.9 -3.0
Cytoplasm -2.4 -1.6 -3.8 -4.9 – -5.0 -5.0 -5.6 -5.9 1.1 3.2 3.6 0.8 -0.3 1.7
Endoplasmic Reticulum -2.4 -1.8 -2.7 0 – 0 -0.7 0 -1.4 0 -1.3 -2.4 -0.7 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 3.4 – 6.0 6.5 8.3 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 4.1 2.5 4.2 4.4 – 3.9 0.8 2.3 2.2 0 0 0 0 2.3 -0.9