Standard name
Human Ortholog
Description Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 1.0 0.96 0.91 0.93 0.92 0.78 0.82 0.71 0.76 0.99 0.98 0.98 – 0.93 0.94 0.84 0.85 0.69 0.89 0.83 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.08 0.09 0.08 0.35 0.24 0.36 0.35 0 0 0 – 0.05 0.05 0.08 0.06 0.15 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 – 0 0 0 5 4 1 1 5
Bud 1 2 1 0 3 1 0 0 2 3 4 4 1 0 0 – 0 0 1 3 2 0 4 5
Bud Neck 0 6 4 1 3 4 6 14 0 2 2 4 1 1 0 – 4 2 2 2 1 0 2 3
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 6 5 1 0 1 1 1 0 0 1 2 4 2 0 – 2 1 1 4 2 1 2 2
Cytoplasm 238 542 148 90 50 194 213 209 126 130 102 150 233 327 337 – 227 171 218 462 105 220 212 217
Endoplasmic Reticulum 1 3 0 0 0 0 0 0 0 0 0 0 0 1 2 – 16 18 4 3 3 1 1 4
Endosome 1 0 0 0 0 0 0 0 0 0 0 1 0 4 2 – 5 4 1 4 1 0 4 2
Golgi 0 3 0 0 0 0 0 0 0 0 0 0 0 0 1 – 4 1 1 1 1 1 2 0
Mitochondria 5 10 2 3 5 17 21 18 56 38 52 70 0 1 0 – 12 9 19 32 22 8 10 13
Nucleus 0 0 1 1 2 2 2 4 0 4 1 3 2 1 2 – 2 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 3 1 0 0 1 4 7 2 3 5 3 0 1 0 – 9 4 1 5 0 2 4 3
Vac/Vac Membrane 2 1 0 0 0 2 2 1 2 2 0 2 1 1 1 – 2 4 3 7 0 0 0 3
Unique Cell Count 246 557 154 90 52 213 228 226 162 158 144 198 235 334 344 245 182 259 543 153 248 255 272
Labelled Cell Count 249 577 162 96 63 222 249 254 189 183 167 239 243 340 345 283 217 259 543 153 248 255 272


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.5 4.3 4.3 3.7 4.1 4.4 4.3 3.3 3.9 3.2 3.6 4.7 4.8 4.4 4.4 5.5 5.6 5.1 5.3 5.6
Std Deviation (1e-4) 0.7 0.8 0.9 1.5 1.3 1.6 2.2 1.6 1.3 2.0 1.1 1.3 1.3 1.3 1.2 1.8 2.6 1.9 1.4 1.8 1.6
Intensity Change (Log2) – – – -0.0 -0.2 -0.07 0.03 -0.01 -0.36 -0.15 -0.42 -0.27 0.15 0.17 0.05 0.04 0.36 0.38 0.25 0.3 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5404 -0.7545 -0.5802 -1.1792 -0.8453 -0.6357 4.697 5.0355 5.0989 4.1435 5.0862 4.8876 1.1847 1.2878 1.302 1.2818 1.2839 1.4213
Actin 0.0236 0.0052 0.0111 0.0002 0.0075 0.0031 0.0535 0.0004 0.0226 0.0001 0.0255 0.0008 0.0183 0.0003 0.0032 0.0003 0.0154 0.0041
Bud 0.0004 0.0062 0.0017 0.0025 0.0195 0.0044 0.0012 0.0002 0.0047 0 0.0035 0.0001 0.0005 0.0008 0.001 0.0059 0.0028 0.0033
Bud Neck 0.002 0.0003 0.0028 0.0002 0.0006 0.002 0.0071 0.0002 0.0009 0.0001 0.0006 0.0009 0.0017 0.0002 0.0037 0.0002 0.0005 0.003
Bud Periphery 0.0011 0.0027 0.0024 0.0008 0.0062 0.0061 0.0028 0.0003 0.01 0 0.0032 0.0002 0.0019 0.0005 0.0048 0.0009 0.0089 0.0016
Bud Site 0.0038 0.0135 0.0052 0.0009 0.0078 0.0003 0.0067 0.0034 0.0126 0 0.0028 0.0001 0.0027 0.0061 0.0056 0.0137 0.0014 0.0005
Cell Periphery 0.0005 0.0006 0.0005 0.0001 0.0003 0.0002 0.0006 0.0004 0.0008 0.0001 0.0006 0.0001 0.0009 0.0003 0.0008 0.0007 0.0005 0.0002
Cytoplasm 0.0912 0.1093 0.1102 0.1223 0.0704 0.1214 0.0739 0.0666 0.1155 0.098 0.085 0.1368 0.0357 0.0521 0.044 0.0675 0.0384 0.0619
Cytoplasmic Foci 0.0112 0.0051 0.0059 0.0055 0.0065 0.0153 0.0241 0.0054 0.006 0.0022 0.0106 0.0065 0.0106 0.0041 0.0052 0.0053 0.0142 0.0072
Eisosomes 0.0007 0.0007 0.0003 0.0001 0.0002 0.0001 0.0006 0.0002 0.0006 0.0001 0.0004 0.0001 0.0009 0.0003 0.0003 0.0001 0.0007 0.0002
Endoplasmic Reticulum 0.0331 0.0162 0.0261 0.0014 0.0024 0.0074 0.0357 0.0025 0.0385 0.0614 0.0055 0.0154 0.0022 0.0013 0.0035 0.0007 0.0026 0.0013
Endosome 0.0066 0.0036 0.0048 0.0006 0.0014 0.0016 0.0151 0.0009 0.0016 0.0014 0.0148 0.0015 0.0085 0.0005 0.0097 0.0004 0.0076 0.0029
Golgi 0.0018 0.0041 0.0006 0 0.0003 0.0002 0.0107 0.0001 0.0004 0 0.0053 0.0005 0.0042 0.0001 0.0023 0.0001 0.0061 0.0008
Lipid Particles 0.0078 0.0011 0.0016 0.0001 0.0005 0.0008 0.0119 0.0005 0.0009 0.0001 0.0027 0.0016 0.0076 0.0002 0.0039 0.0009 0.0162 0.0032
Mitochondria 0.0019 0.0037 0.0022 0.0002 0.0324 0.0012 0.0054 0.0004 0.0011 0.0003 0.0046 0.0003 0.0057 0.0003 0.0027 0.0004 0.0201 0.0011
None 0.7979 0.818 0.8111 0.8289 0.8373 0.8242 0.7135 0.9135 0.7609 0.8263 0.774 0.828 0.8768 0.9288 0.8938 0.899 0.8278 0.8925
Nuclear Periphery 0.0039 0.0009 0.0028 0.0009 0.0009 0.0012 0.0062 0.0006 0.0038 0.0053 0.022 0.0023 0.0035 0.0003 0.004 0.0002 0.001 0.0033
Nucleolus 0.0005 0.0006 0.0008 0.0022 0.0003 0.0005 0.0015 0.0003 0.0005 0.0003 0.0013 0.0001 0.0006 0.0003 0.0022 0.0007 0.0013 0.0006
Nucleus 0.0014 0.0022 0.0025 0.0071 0.0019 0.0028 0.0068 0.0012 0.002 0.0025 0.0104 0.0007 0.0009 0.001 0.0025 0.0012 0.0012 0.0016
Peroxisomes 0.0055 0.0013 0.0006 0.0001 0.0007 0.0005 0.0132 0.0003 0.0072 0.0001 0.0067 0.0008 0.0088 0.0003 0.0014 0.0003 0.0119 0.0037
Punctate Nuclear 0.0028 0.0035 0.0021 0.0256 0.0023 0.0055 0.0055 0.0014 0.0069 0.0005 0.0149 0.0024 0.0063 0.0015 0.0026 0.0007 0.0195 0.0051
Vacuole 0.0018 0.0011 0.0031 0.0003 0.0005 0.0008 0.0029 0.0009 0.0018 0.001 0.004 0.0004 0.0013 0.0006 0.002 0.0005 0.0012 0.0016
Vacuole Periphery 0.0005 0.0002 0.0017 0.0001 0.0001 0.0002 0.001 0.0001 0.0006 0.0002 0.0017 0.0001 0.0006 0 0.0007 0.0001 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.9437 131.5168 134.9054 97.0578 71.9981 33.1559 65.1846 48.3947 44.3227 45.5497
Translational Efficiency 1.354 1.121 1.076 1.36 1.8167 1.0922 1.2013 1.2929 1.5387 1.3472

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tir1

Tir1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tir1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available