Standard name
Human Ortholog
Description Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.97 0.95 0.91 0.79 0.79 0.78 0.67 0.63 0.59 0.59 0.98 0.98 0.99 0.97 0.97 0.95 0.83 0.83 0.7 0.84 0.82 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0 0.07 0.11 0.43 0.35 0.32 0.51 0.58 0.63 0.59 0 0 0 0 0 0 0.07 0.07 0.14 0.07 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 0 2 3 0 0 0 0 0 0 0 2 2 10 2 3 10
Bud 3 0 3 3 5 11 9 14 17 8 12 15 0 3 2 0 0 0 2 3 10 1 14 7
Bud Neck 1 1 1 0 5 1 0 2 1 2 2 1 0 1 0 2 3 1 1 1 4 1 2 4
Bud Site 0 0 1 0 0 0 0 1 2 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 2 1 1 0 6 6 11 5 6 8 3 10 16 6 7 10 1 3 5 2 3 5
Cytoplasm 329 392 507 334 362 419 553 630 364 366 206 211 473 677 604 344 289 249 283 345 366 198 354 295
Endoplasmic Reticulum 0 2 3 3 2 1 0 3 0 7 4 3 2 2 0 33 29 17 2 0 7 3 3 1
Endosome 0 1 1 1 6 4 7 5 3 0 0 0 2 0 3 4 2 6 6 7 10 0 1 2
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 2 1 5 2 0 0 0
Mitochondria 67 10 17 25 44 226 244 262 274 340 221 211 0 0 2 10 5 3 23 28 73 16 23 26
Nucleus 0 0 0 0 0 2 4 1 2 1 1 1 1 2 0 2 4 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 2 1 2 1 0 0 2 0 0 1 1 0 0 0 1 0 1 0 2 0
Nucleolus 0 0 2 0 0 1 0 2 1 0 1 1 1 1 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 1 3 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0 1 0
SpindlePole 1 1 4 1 2 3 16 16 8 6 4 4 0 1 1 0 2 2 2 3 5 2 7 6
Vac/Vac Membrane 4 3 1 1 5 5 3 5 4 6 2 6 2 4 4 7 4 1 5 7 6 1 6 15
Unique Cell Count 349 406 523 350 399 529 704 810 540 582 351 355 481 689 613 354 297 261 341 419 525 235 431 384
Labelled Cell Count 408 415 543 372 435 675 847 949 687 745 466 462 486 703 634 408 347 292 341 419 525 235 431 384


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.9 3.6 3.6 3.6 3.2 3.0 3.4 3.1 2.9 3.2 3.2 4.4 4.4 4.5 5.5 5.9 5.9 4.2 4.4 5.3
Std Deviation (1e-4) 1.2 0.7 1.0 1.5 1.6 1.4 1.3 1.4 1.1 1.0 1.3 1.4 1.5 1.3 1.5 1.8 1.8 2.0 1.2 1.4 1.9
Intensity Change (Log2) – – – -0.01 0.01 -0.16 -0.29 -0.08 -0.22 -0.34 -0.19 -0.19 0.29 0.28 0.31 0.6 0.72 0.7 0.21 0.29 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2538 -0.5501 -0.5863 -0.8056 -1.1986 -0.572 2.4112 1.6013 1.953 1.4728 1.98 2.0589 2.1534 3.1398 2.5185 2.1829 2.3372 2.6412
Actin 0.0455 0.0003 0.0175 0.0022 0.0018 0.0028 0.067 0.0003 0.0105 0.0002 0.0446 0.0054 0.0037 0.0005 0.0009 0 0.0002 0.0002
Bud 0.0006 0.0022 0.0009 0.0007 0.0001 0.0011 0.0017 0.0067 0.0054 0.0348 0.0011 0.0065 0.0002 0.0038 0.0013 0 0 0.0012
Bud Neck 0.0032 0.0007 0.0013 0 0.0001 0.0005 0.0008 0 0.007 0.0006 0.0001 0.0011 0.0007 0 0.0035 0 0.0005 0.0008
Bud Periphery 0.0014 0.0031 0.0019 0.0023 0.0001 0.0051 0.0069 0.0011 0.0032 0.0235 0.0016 0.0035 0.0005 0.003 0.0025 0 0.0001 0.0024
Bud Site 0.0061 0.0125 0.0037 0.0001 0.0002 0.0004 0.003 0.0057 0.0171 0.0029 0.001 0.001 0.0007 0.0037 0.001 0 0.0001 0.0002
Cell Periphery 0.0013 0.0002 0.0002 0.0001 0.0001 0.0002 0.0006 0.0002 0.0009 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0222 0.0293 0.0426 0.04 0.0722 0.0691 0.0288 0.0336 0.021 0.0323 0.0213 0.0458 0.0199 0.0246 0.0234 0.0325 0.0482 0.0457
Cytoplasmic Foci 0.0373 0.0076 0.018 0.0048 0.0365 0.0247 0.0404 0.0039 0.0129 0.0069 0.0178 0.032 0.0078 0.0024 0.0129 0.0014 0.0054 0.0024
Eisosomes 0.0009 0.0002 0.0003 0.0002 0.0002 0.0002 0.0007 0.0002 0.0004 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0077 0.0004 0.0015 0.0025 0.0011 0.0013 0.0016 0.0003 0.0012 0.0005 0.0004 0.0005 0.0013 0.0002 0.0004 0.0002 0.0007 0.0002
Endosome 0.0326 0.0005 0.0013 0.0017 0.0138 0.003 0.033 0.0025 0.0045 0.0011 0.0029 0.0083 0.0067 0.0001 0.003 0.0003 0.002 0.0001
Golgi 0.0116 0.0002 0.0012 0.0002 0.0035 0.0008 0.009 0.0006 0.0023 0.0001 0.0053 0.0056 0.002 0 0.0006 0 0.0001 0
Lipid Particles 0.0338 0.0005 0.0023 0.0004 0.0121 0.006 0.0232 0.0027 0.0027 0.0003 0.0057 0.0112 0.0103 0.0001 0.0008 0.0001 0.0006 0.0001
Mitochondria 0.0108 0.0005 0.001 0.0006 0.0486 0.0025 0.0076 0.0067 0.0093 0.0012 0.0014 0.0033 0.0038 0.0003 0.0005 0.0001 0.0001 0.0001
None 0.7016 0.9358 0.8862 0.942 0.8029 0.8759 0.7234 0.9318 0.8686 0.8793 0.8371 0.8429 0.9234 0.9597 0.9419 0.9644 0.9399 0.9454
Nuclear Periphery 0.0258 0.0002 0.0008 0.0007 0.0005 0.0005 0.0045 0.0002 0.0019 0.0006 0.0007 0.0024 0.0075 0.0002 0.0007 0.0001 0.0002 0.0001
Nucleolus 0.0019 0.0016 0.0056 0.0001 0.0002 0.0002 0.0006 0.0004 0.0022 0.0026 0.0004 0.002 0.0007 0.0001 0.0005 0 0.0001 0.0001
Nucleus 0.0108 0.0007 0.0007 0.0003 0.0002 0.0005 0.0029 0.001 0.0081 0.0075 0.0011 0.0018 0.002 0.0003 0.0008 0.0001 0.0003 0.0002
Peroxisomes 0.0211 0.001 0.0099 0.0003 0.0032 0.0027 0.028 0.001 0.0031 0.0003 0.0389 0.0183 0.0036 0.0001 0.0018 0 0.0001 0.0001
Punctate Nuclear 0.0169 0.0014 0.0024 0.0003 0.0014 0.0016 0.0138 0.0004 0.0161 0.0027 0.0175 0.0066 0.0035 0.0004 0.0027 0.0001 0.0002 0.0002
Vacuole 0.0031 0.0008 0.0007 0.0004 0.0005 0.0008 0.0018 0.0007 0.0013 0.0019 0.0004 0.0009 0.0008 0.0003 0.0004 0.0002 0.0009 0.0003
Vacuole Periphery 0.0038 0.0001 0.0002 0.0001 0.0005 0.0002 0.0008 0.0002 0.0003 0.0003 0.0001 0.0005 0.0005 0.0001 0.0001 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.6749 1.1978 2.6664 2.7687 0.4343 4.0175 1.968 1.8391 3.4134 2.7426
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Gip2

Gip2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gip2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available