Standard name
Human Ortholog
Description Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0.06 0.07 0.1 0.09 0.09 0.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.32 – – 0.1 0.09 0.14 0.15 0.14 0.15
Mitochondria 0.8 0.72 0.71 0.82 0.83 0.89 0.86 0.86 0.95 0.88 0.87 0.94 0.71 0.69 0.53 0.05 – – 0.48 0.48 0.37 0.4 0.31 0.23
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0.35 0.27 0.26 0.25 0.14 0.22 0.19 0.2 0.15 0.18 0.13 0.13 0.27 0.34 0.37 0.47 – – 0.13 0.1 0.08 0.11 0.11 0.1
Peroxisomes 0 0 0.13 0.06 0 0 0.05 0 0 0 0 0 0.11 0.14 0.12 0.05 – – 0.07 0.07 0.15 0.11 0.14 0.17
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.07 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0.08 0 – – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 0 0 0 1 1 1 0 0 0 0 0 0 1 2 – – 16 14 15 8 15 19
Bud 1 1 3 0 1 2 2 3 3 2 1 2 1 2 1 0 – – 3 4 0 1 1 7
Bud Neck 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 8 3 1 1 7 4
Bud Site 0 0 1 0 0 0 0 0 1 2 1 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 2 0 0 0 0 1 0 1 3 0 0 0 0 1 0 – – 0 0 0 0 0 0
Cytoplasm 4 1 2 0 2 5 1 7 1 2 1 2 4 1 3 1 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 0 – – 0 1 0 0 0 1
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 – – 4 6 5 3 8 6
Golgi 0 5 1 0 2 0 1 1 0 0 0 0 0 0 0 6 – – 28 17 21 14 26 29
Mitochondria 206 102 80 73 86 90 156 156 109 167 68 119 80 75 74 1 – – 132 94 56 37 57 44
Nucleus 3 1 1 0 0 1 1 2 0 1 1 0 1 2 1 0 – – 0 1 0 0 0 0
Nuclear Periphery 3 1 0 0 0 1 2 2 0 2 0 1 0 0 0 0 – – 1 0 0 0 1 0
Nucleolus 91 38 29 22 15 22 34 37 17 35 10 17 30 37 52 9 – – 35 19 12 10 19 19
Peroxisomes 5 6 15 5 4 2 9 3 1 5 2 2 12 15 17 1 – – 20 14 22 10 25 33
SpindlePole 5 3 0 0 0 0 1 1 0 1 1 0 1 2 2 2 – – 6 5 4 2 9 13
Vac/Vac Membrane 18 8 5 2 6 3 6 4 0 4 2 0 5 2 11 0 – – 9 9 5 2 5 8
Unique Cell Count 257 141 112 89 104 101 181 181 115 190 78 126 112 109 140 19 278 197 153 94 182 196
Labelled Cell Count 338 175 137 102 116 127 215 217 136 224 87 143 134 137 167 23 278 197 153 94 182 196


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.7 28.1 28.3 36.6 35.5 29.8 31.6 26.7 28.3 24.6 26.9 28.6 38.4 37.2 34.8 45.6 – – 22.5 24.7 26.0
Std Deviation (1e-4) 16.1 13.8 13.9 16.4 14.4 13.0 14.7 10.6 11.5 11.6 15.8 13.1 19.8 16.4 19.1 41.5 – – 13.5 10.0 12.1
Intensity Change (Log2) – – – 0.37 0.33 0.08 0.16 -0.08 0.0 -0.2 -0.07 0.02 0.44 0.39 0.3 0.69 – – -0.33 -0.19 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 1.7 2.0 3.2 3.1 3.1 4.7 3.6 2.6 4.8 0 -0.4 -3.0 -5.5 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus -0.2 -2.1 -0.7 -1.4 -1.1 -2.1 -1.5 -2.2 -2.4 0.2 1.3 1.9 1.9 – –
Peroxisomes -1.8 -2.5 -3.1 -2.6 -4.1 -3.7 -3.6 -2.6 -3.5 -0.6 0.1 -0.3 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 0 0.4 0 0 0 0 0 0 0 0 0 1.1 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0118 0.0004 0.0015 0.0044 0.0338 0.0017 0.0002 0.0002 0.0001 0 0.0001 0.0001 – – – – – –
Bud 0.0001 0.0001 0.0003 0 0.0004 0 0 0 0 0 0 0 – – – – – –
Bud Neck 0.0002 0 0.0001 0.0001 0.0029 0 0 0 0 0 0 0 – – – – – –
Bud Periphery 0.0001 0.0001 0.0007 0.0001 0.0005 0.0002 0 0 0 0 0 0 – – – – – –
Bud Site 0.0005 0.0003 0.0005 0.0001 0.007 0.0001 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.0004 0.0008 0.0012 0.0004 0.0005 0.0001 0.0001 0.0004 0.0001 0 0 0 – – – – – –
Cytoplasm 0 0 0.0003 0 0.0001 0 0 0 0 0 0 0 – – – – – –
Cytoplasmic Foci 0.0222 0.0004 0.0003 0 0.0195 0.0013 0 0.0003 0.0001 0 0 0.0001 – – – – – –
Eisosomes 0.0008 0.0003 0.0027 0.0033 0.0006 0.0002 0.0012 0.0003 0.0005 0 0.0002 0.0002 – – – – – –
Endoplasmic Reticulum 0 0 0.0006 0 0 0.0002 0 0 0 0 0 0 – – – – – –
Endosome 0.0005 0.0001 0.0064 0.0001 0.0013 0.0035 0 0.0002 0 0 0 0 – – – – – –
Golgi 0.0025 0.0001 0.0006 0.0012 0.0043 0.0037 0.0001 0.0053 0.0001 0 0.0001 0.0001 – – – – – –
Lipid Particles 0.0411 0.0024 0.0012 0.0056 0.0458 0.0343 0.0027 0.0073 0.0058 0.0001 0.0017 0.0022 – – – – – –
Mitochondria 0.773 0.9661 0.9517 0.9726 0.8067 0.944 0.9882 0.9679 0.9786 0.9976 0.9865 0.9792 – – – – – –
None 0.0001 0 0.0009 0 0.0003 0.0002 0 0 0 0 0 0 – – – – – –
Nuclear Periphery 0.0002 0 0.009 0.0002 0.0001 0.0021 0 0 0 0 0 0.0001 – – – – – –
Nucleolus 0.0283 0.0136 0.0014 0.0003 0.0027 0.0003 0.0005 0.0009 0.0024 0 0.0001 0.0001 – – – – – –
Nucleus 0.0015 0.0005 0.0026 0.0001 0.0003 0.0002 0 0.0001 0.0001 0 0 0 – – – – – –
Peroxisomes 0.1115 0.0036 0.0036 0.0006 0.0614 0.0045 0.0013 0.0036 0.0014 0.0003 0.0004 0.002 – – – – – –
Punctate Nuclear 0.0016 0 0.0002 0 0.0017 0.0009 0 0 0 0 0 0 – – – – – –
Vacuole 0.0005 0.0036 0.0056 0.0007 0.0013 0.0004 0.0003 0.0018 0.0003 0 0.0002 0.0002 – – – – – –
Vacuole Periphery 0.0031 0.0077 0.0087 0.0101 0.0088 0.002 0.0054 0.0116 0.0104 0.0017 0.0106 0.0157 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.7138 56.166 44.019 45.2211 45.3073 69.8507 61.256 52.9716 53.0534 57.9869
Translational Efficiency 1.1125 0.9944 1.0651 1.3033 1.1099 1.1498 1.101 1.0115 1.0302 0.8633

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
Localization
Cell Percentages mitochondrion (35%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Ald5

Ald5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ald5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available