ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.94 1.0 0.97 0.94 – 0.79 0.74 0.73 0.69 0.73 1.0 1.0 0.98 0.94 0.97 0.9 0.97 0.96 0.97 0.94 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.12 0 0.2 – 0.29 0.54 0.48 0.51 0.45 0 0 0 0.08 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 1 0 0 0 – 3 0 1 1 6 2 0 0 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 0 – 1 1 2 0 0 0 0 0 1 1 6 0 0 0 0 0 0
Cytoplasm 265 299 16 26 107 79 – 221 137 141 50 108 183 182 218 34 65 61 261 295 54 36 159 218
Endoplasmic Reticulum 0 2 0 0 1 0 – 0 0 0 0 1 0 0 1 1 2 4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1
Golgi 2 0 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0
Mitochondria 5 1 1 3 4 17 – 82 100 92 37 67 0 1 0 3 2 0 2 3 0 0 1 0
Nucleus 0 2 0 0 0 0 – 5 0 2 2 2 3 1 1 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 0 – 9 3 6 1 3 0 0 0 0 1 6 0 0 0 0 1 1
Unique Cell Count 270 302 17 26 110 84 279 186 193 72 148 183 182 222 36 67 68 270 306 56 39 169 228
Labelled Cell Count 273 306 18 29 115 97 321 242 246 92 187 188 184 222 41 71 79 270 306 56 39 169 228


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.2 4.4 5.2 5.5 4.5 – 4.5 3.7 4.2 3.8 4.1 7.0 7.2 7.0 12.2 13.2 13.9 – 7.4 7.5
Std Deviation (1e-4) 1.0 1.0 0.8 1.2 1.1 0.7 – 0.9 0.7 1.0 0.8 2.0 1.0 1.0 1.1 4.2 2.7 2.9 – 1.2 1.4
Intensity Change (Log2) – – – 0.24 0.3 0.01 – 0.03 -0.24 -0.09 -0.23 -0.11 0.66 0.69 0.66 1.47 1.58 1.65 – 0.74 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.7 0 – -1.5 -1.9 -1.9 -2.1 -1.9 3.3 3.3 1.1 0 0.6 -0.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2746 3.2625 3.1149 2.3943 2.2877 2.9209 2.4557 3.0772 2.687 2.5801 2.4529 2.6371 3.4027 4.6337 4.3516 3.9204 3.897 4.1828
Actin 0.0148 0.0005 0.0049 0.0372 0.002 0.009 0.0262 0.0002 0.0058 0.001 0.0154 0.0046 0.0136 0.0003 0.0055 0.0062 0.0064 0.0014
Bud 0.001 0.0085 0.0002 0.002 0.0001 0.0001 0.003 0.0097 0.0005 0.0004 0.0015 0.0014 0.0003 0.0003 0.0002 0.0025 0.0003 0.0002
Bud Neck 0.0078 0.0002 0.0006 0.0013 0.0004 0.0007 0.0048 0.0001 0.0008 0.0006 0.0009 0.0015 0.0023 0.0001 0.0003 0.0016 0.0006 0.001
Bud Periphery 0.0007 0.0039 0.0002 0.0015 0 0.0002 0.0033 0.0025 0.0007 0.0008 0.0016 0.0012 0.0003 0.0001 0.0001 0.0044 0.0004 0.0001
Bud Site 0.008 0.0048 0.0008 0.0129 0.0001 0.0001 0.0099 0.0009 0.0012 0.0008 0.0012 0.0012 0.002 0.0007 0.0005 0.008 0.0021 0.0002
Cell Periphery 0.0003 0.0003 0.0001 0.0002 0 0 0.0004 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0005 0.0001 0
Cytoplasm 0.7377 0.9139 0.8604 0.804 0.8697 0.896 0.6421 0.9148 0.883 0.8301 0.8007 0.8873 0.8427 0.9466 0.9111 0.7788 0.8585 0.95
Cytoplasmic Foci 0.0269 0.0029 0.0129 0.0237 0.0077 0.0116 0.0467 0.0031 0.0147 0.0295 0.0244 0.0242 0.024 0.0035 0.0037 0.0164 0.0058 0.0075
Eisosomes 0.0003 0 0 0.0014 0.0002 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.004 0.0016 0.0028 0.0021 0.0019 0.0031 0.0075 0.0011 0.002 0.0053 0.001 0.0018 0.0033 0.0014 0.002 0.0048 0.0013 0.0018
Endosome 0.021 0.0012 0.01 0.0056 0.0029 0.0105 0.0603 0.0009 0.0136 0.0366 0.0134 0.0104 0.0073 0.0012 0.0022 0.0267 0.0031 0.0071
Golgi 0.0052 0.0001 0.0013 0.0092 0.0004 0.0037 0.0198 0.0001 0.0067 0.0056 0.0159 0.0035 0.0024 0.0001 0.0003 0.0056 0.0022 0.0006
Lipid Particles 0.0127 0 0.0022 0.0103 0.0015 0.0039 0.0143 0 0.0024 0.0069 0.0252 0.0016 0.0038 0 0.0003 0.0059 0.0053 0.0002
Mitochondria 0.0042 0.0001 0.0036 0.0018 0.0002 0.0049 0.017 0.0001 0.0062 0.0248 0.0175 0.0057 0.0011 0.0001 0.0003 0.0041 0.0024 0.0004
None 0.1296 0.0527 0.0837 0.0652 0.0819 0.0447 0.0743 0.0633 0.0502 0.0334 0.0508 0.0435 0.0648 0.0409 0.0629 0.0772 0.1009 0.0205
Nuclear Periphery 0.0018 0.001 0.0012 0.0011 0.0031 0.0038 0.013 0.0006 0.0011 0.0026 0.0006 0.0008 0.002 0.0007 0.0036 0.0191 0.0006 0.0018
Nucleolus 0.0013 0.0001 0 0.0004 0.001 0.0002 0.0021 0 0.0001 0.0003 0.0001 0.0002 0.0002 0 0.0001 0.0008 0.0002 0
Nucleus 0.0027 0.0053 0.0023 0.0024 0.0037 0.0022 0.0159 0.0017 0.003 0.0069 0.0023 0.0044 0.0076 0.0029 0.0037 0.0052 0.0024 0.0045
Peroxisomes 0.0138 0 0.0089 0.0156 0.0011 0.0022 0.0217 0 0.0051 0.0057 0.0261 0.0035 0.0089 0.0001 0.0009 0.002 0.0055 0.0002
Punctate Nuclear 0.0037 0.0015 0.0013 0.0017 0.0213 0.0006 0.0103 0.0003 0.0014 0.0042 0.0007 0.0021 0.0123 0.0005 0.0016 0.0228 0.0011 0.0011
Vacuole 0.002 0.0012 0.0021 0.0005 0.0007 0.0012 0.0048 0.0005 0.0009 0.0034 0.0004 0.0008 0.0006 0.0004 0.0004 0.0051 0.0005 0.0008
Vacuole Periphery 0.0004 0.0001 0.0006 0.0002 0.0002 0.0011 0.0024 0.0001 0.0004 0.001 0.0003 0.0003 0.0002 0.0001 0.0002 0.0022 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1985 1054 221 135 1635 1871 186 1461 3620 2925 407 1596

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 774.52 869.23 1146.48 1230.20 689.25 854.45 1006.82 1080.98 736.01 859.78 1082.66 1093.60
Standard Deviation 98.49 121.12 133.72 136.34 88.85 125.12 141.17 163.23 103.37 123.89 153.81 166.39
Intensity Change Log 2 — 0.166435 0.565837 0.667518 — 0.309969 0.546707 0.649241 — 0.235803 0.556860 0.658941

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000240 0.001068 0.000374 0.000523 0.000165 0.000735 0.002665 0.000279 0.000192 0.000916 0.002471
Bud Neck 0.004574 0.003078 0.003465 0.002262 0.012273 0.007866 0.002662 0.007850 0.008051 0.006141 0.003098 0.007377
Bud Site 0.003324 0.009335 0.007813 0.011464 0.009153 0.009762 0.010613 0.036911 0.005957 0.009608 0.009093 0.034759
Cell Periphery 0.000078 0.000104 0.000377 0.000133 0.000230 0.000060 0.000197 0.000090 0.000147 0.000076 0.000295 0.000094
Cytoplasm 0.664646* 0.714359* 0.422445* 0.528514* 0.560829* 0.685626* 0.546628* 0.648799* 0.617756* 0.695979* 0.479197* 0.638625*
Cytoplasmic Foci 0.150316 0.116804 0.023448 0.024905 0.254815* 0.116520 0.017147 0.025050 0.197514* 0.116622 0.020568 0.025038
Eisosomes 0.000057 0.000037 0.000084 0.000036 0.000206 0.000038 0.000040 0.000024 0.000124 0.000038 0.000064 0.000025
Endoplasmic Reticulum 0.000670 0.000772 0.007921 0.001312 0.001697 0.000804 0.004643 0.000684 0.001134 0.000793 0.006423 0.000737
Endosome 0.007409 0.008444 0.007879 0.005131 0.009682 0.009577 0.004881 0.013409 0.008436 0.009169 0.006509 0.012709
Golgi 0.001313 0.001026 0.000343 0.000167 0.004212 0.001832 0.000223 0.009586 0.002622 0.001541 0.000288 0.008789
Lipid Particles 0.002902 0.001453 0.003683 0.001707 0.005404 0.001244 0.001301 0.000720 0.004032 0.001320 0.002594 0.000803
Mitochondria 0.001088 0.002205 0.000411 0.000101 0.006112 0.002961 0.001256 0.012254 0.003357 0.002689 0.000797 0.011226
Mitotic Spindle 0.001693 0.002876 0.002505 0.011523 0.000889 0.007830 0.003285 0.046178 0.001330 0.006045 0.002862 0.043247
None 0.003855 0.002707 0.001896 0.001858 0.002488 0.002390 0.004836 0.004982 0.003238 0.002504 0.003240 0.004718
Nuclear Periphery 0.000182 0.000167 0.001792 0.000685 0.000543 0.000217 0.000748 0.000301 0.000345 0.000199 0.001315 0.000334
Nuclear Periphery Foci 0.000647 0.001663 0.010033 0.006431 0.000567 0.000698 0.006685 0.001985 0.000611 0.001046 0.008503 0.002361
Nucleolus 0.000621 0.000528 0.000409 0.000112 0.000524 0.000850 0.000190 0.000149 0.000577 0.000734 0.000309 0.000146
Nucleus 0.091782 0.060314 0.263046* 0.049613 0.087967 0.060556 0.267140* 0.064613 0.090059 0.060469 0.264917* 0.063344
Peroxisomes 0.001285 0.001285 0.000386 0.000309 0.002889 0.002260 0.000873 0.003515 0.002009 0.001908 0.000608 0.003243
Vacuole 0.062862 0.071741 0.240591* 0.353102* 0.036532 0.087821 0.125466 0.117293 0.050970 0.082027 0.187978 0.137239
Vacuole Periphery 0.000618 0.000861 0.000406 0.000258 0.002467 0.000925 0.000451 0.002941 0.001453 0.000902 0.000427 0.002714

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.74 -11.17 -6.47 -2.32 7.26 1.20 -0.97 -0.59 -2.64 0.37 0.63 -5.02 -1.81 -2.60 1.56
Bud Neck 3.37 -0.17 5.03 1.60 4.28 3.76 7.41 9.11 5.39 0.37 2.93 6.42 8.37 4.87 1.96
Bud Site -4.44 -7.70 -4.39 -0.31 -0.24 -0.31 0.04 -2.12 -1.75 -1.39 -3.88 -2.87 -4.62 -1.93 -1.87
Cell Periphery -2.49 -9.34 -3.66 -0.89 7.76 4.64 0.63 4.38 -2.66 7.01 4.18 -5.69 4.39 0.53 11.31
Cytoplasm -4.88 20.34 13.71 15.22 -0.04 -14.19 5.94 1.49 14.49 -5.15 -11.97 18.01 10.81 19.27 -10.23
Cytoplasmic Foci 6.04 32.03 30.57 20.55 1.24 22.98 44.45 46.36 28.24 -0.99 19.84 52.62 55.15 35.71 0.63
Eisosomes 6.34 -5.33 5.21 -0.27 8.07 6.48 6.01 7.01 7.88 6.09 7.26 4.28 8.32 8.47 11.84
Endoplasmic Reticulum -1.24 -12.42 -4.09 -3.25 11.19 2.17 -4.69 3.44 0.90 7.57 1.55 -12.17 3.31 1.03 14.19
Endosome -1.22 -0.42 8.08 6.95 6.91 0.31 6.01 7.27 7.02 0.31 -0.98 4.34 7.75 7.91 3.48
Golgi 1.45 6.22 7.85 4.92 3.84 5.69 12.72 4.24 0.14 -2.93 4.42 13.97 2.17 -0.13 -2.75
Lipid Particles 3.40 -1.60 2.16 -1.98 4.81 12.15 10.63 13.43 3.23 4.88 9.94 4.10 11.50 3.32 9.27
Mitochondria -0.96 2.37 3.63 1.95 3.60 3.09 6.66 3.76 0.59 -1.83 1.12 7.01 1.73 0.58 -2.11
Mitotic Spindle -0.84 -1.31 -1.81 -1.64 -1.62 -4.43 -1.79 -7.56 -3.85 -6.61 -4.19 -2.13 -7.56 -4.99 -6.88
None 1.75 4.59 3.01 1.00 -0.59 0.52 -2.62 -5.45 -5.51 0.44 2.19 0.23 -2.78 -4.92 -2.28
Nuclear Periphery 0.03 -16.60 -11.07 -11.09 7.49 0.89 -1.53 -0.59 -9.16 3.80 0.84 -7.55 -2.67 -13.54 10.73
Nuclear Periphery Foci -6.66 -11.11 -7.89 -6.02 4.24 -0.36 -8.55 -3.26 -4.18 7.16 -3.18 -13.86 -5.88 -4.24 10.99
Nucleolus 0.50 1.61 3.50 2.66 2.67 -1.38 5.11 5.74 2.97 1.17 -0.86 3.09 4.96 3.62 2.40
Nucleus 9.14 -12.89 5.24 0.01 14.18 8.13 -12.57 -2.73 -11.15 11.93 12.59 -18.01 -1.73 -11.42 17.28
Peroxisomes 0.01 6.16 7.00 7.45 2.60 1.58 5.93 5.40 4.97 -1.18 0.49 7.85 5.21 5.06 -1.69
Vacuole -3.73 -15.20 -18.63 -17.95 -8.03 -17.26 -9.91 -34.83 -25.32 -4.32 -13.87 -17.53 -35.87 -29.83 -3.59
Vacuole Periphery -1.00 2.93 8.83 3.38 3.06 3.02 4.27 3.43 1.29 -1.13 2.30 4.75 2.97 1.47 -0.87
ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YER084W

YER084W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yer084w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available