Standard name
Human Ortholog
Description 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.27 0.25 – 0 0 0 0.07 0.06 0 0 0 0.26 0.23 0.25 – 0.28 0.32 0.09 0.16 0.1 0.17 0.13 0.11
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0.1 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.5 0 – 0 0.15 0.23 0.16 0.51 0.49 0.45 0.52 0.11 0 0 – 0.08 0 0 0 0 0 0 0.06
Nucleus 0.68 0.38 0.75 – 0.96 0.87 0.95 0.87 0.81 0.83 0.82 0.81 0.66 0.71 0.7 – 0.5 0.51 0.66 0.48 0.62 0.51 0.46 0.36
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0.06 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07
Vac/Vac Membrane 0.24 0.1 0.05 – 0 0 0 0 0 0 0 0 0 0.08 0.07 – 0.22 0.13 0.17 0.26 0.19 0.21 0.28 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Bud 0 1 0 – 0 0 0 1 1 4 2 2 0 0 0 – 0 0 1 3 0 4 4 6
Bud Neck 0 1 0 – 0 0 0 0 1 1 1 0 0 0 0 – 0 0 0 2 0 0 0 1
Bud Site 0 0 0 – 0 1 1 2 8 6 3 17 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 16 91 5 – 3 1 3 8 12 5 8 10 32 69 80 – 43 57 13 61 4 38 29 27
Endoplasmic Reticulum 0 2 0 – 0 0 0 0 0 0 0 0 0 0 1 – 5 17 0 0 0 0 0 1
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 3 4 – 5 3 0 0 0 0 0 5
Golgi 1 1 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 2 0 0 0 0
Mitochondria 16 166 0 – 0 8 18 19 107 93 102 125 14 5 4 – 13 5 3 7 0 4 3 15
Nucleus 86 126 15 – 88 46 75 102 171 159 187 193 81 214 222 – 78 91 99 185 26 118 106 89
Nuclear Periphery 0 0 0 – 0 0 0 0 2 2 1 2 0 0 0 – 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 – 0 3 2 4 12 6 9 9 2 0 0 – 0 1 0 0 0 0 3 7
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 1 0 0 0 1 0 1 1 0 3 – 0 3 2 8 1 9 10 17
Vac/Vac Membrane 30 32 1 – 1 2 0 1 1 0 1 3 4 23 23 – 34 24 26 102 8 48 64 60
Unique Cell Count 126 331 20 92 53 79 117 210 191 228 239 123 302 318 155 178 150 390 43 234 230 245
Labelled Cell Count 149 421 21 92 62 99 137 315 277 314 362 134 314 337 178 201 150 390 43 234 230 245


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.4 5.4 – 6.5 4.8 4.7 5.3 4.2 4.1 4.3 4.2 4.8 5.4 5.3 4.2 5.5 5.2 5.4 5.9 5.6
Std Deviation (1e-4) 0.5 0.8 0.8 – 1.0 1.3 1.2 1.3 1.3 1.2 1.0 0.9 1.3 1.1 1.6 1.2 1.9 1.7 1.4 2.0 1.3
Intensity Change (Log2) – – – – 0.29 -0.17 -0.2 -0.02 -0.36 -0.37 -0.33 -0.35 -0.16 0.0 -0.01 -0.33 0.03 -0.04 0.02 0.14 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.986 3.884 3.5344 3.5422 3.3523 3.93 3.9443 3.8045 3.274 2.9705 3.3099 2.9164 0.0098 0.3919 -0.3133 0.1027 -0.2379 -0.0458
Actin 0.0509 0.0002 0.0074 0.0012 0.0186 0.0027 0.0141 0.0002 0.0013 0.0015 0.0024 0.0003 0.0345 0.0008 0.0002 0.0116 0.0345 0.0009
Bud 0.0023 0.0002 0.0002 0 0.0005 0.0001 0.0002 0 0.0006 0.0005 0.0003 0.0001 0.0004 0.0005 0.0001 0.0003 0.0002 0.0002
Bud Neck 0.0172 0.0007 0.0004 0.0005 0.0007 0.003 0.0004 0.0003 0.0008 0.0008 0.0007 0.0045 0.0037 0.0006 0.0007 0.001 0.0107 0.0047
Bud Periphery 0.0041 0.0002 0.0004 0.0001 0.0008 0.0001 0.0002 0 0.0008 0.0005 0.0004 0.0001 0.0007 0.001 0.0001 0.0005 0.0003 0.0004
Bud Site 0.0115 0.0009 0.0005 0.0004 0.0011 0.0003 0.0005 0.0005 0.0048 0.0022 0.0005 0.0003 0.0061 0.0017 0.0005 0.0006 0.0012 0.0006
Cell Periphery 0.0006 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0568 0.0657 0.0415 0.0246 0.02 0.0443 0.0261 0.0795 0.0818 0.0885 0.0684 0.0638 0.0951 0.1156 0.0994 0.0505 0.102 0.1003
Cytoplasmic Foci 0.021 0.0012 0.0037 0.0119 0.0063 0.0024 0.0347 0.0015 0.0129 0.0382 0.0211 0.0014 0.0234 0.0026 0.0018 0.0023 0.0121 0.0045
Eisosomes 0.0008 0.0001 0.0001 0.0002 0.0002 0.0002 0.0023 0 0 0 0.0007 0 0.001 0.0001 0 0.0002 0.0004 0
Endoplasmic Reticulum 0.0086 0.0023 0.0011 0.0008 0.0006 0.0016 0.0069 0.0018 0.003 0.0015 0.0047 0.0021 0.0059 0.0049 0.0025 0.0044 0.0023 0.0032
Endosome 0.0175 0.0023 0.003 0.0037 0.0079 0.0044 0.0503 0.0022 0.0378 0.0664 0.0543 0.0027 0.0155 0.0045 0.0019 0.0137 0.0125 0.0053
Golgi 0.0064 0.0001 0.0016 0.0024 0.0116 0.0004 0.0053 0 0.0268 0.0162 0.0461 0.0004 0.0049 0.0001 0.0001 0.0088 0.0079 0.0005
Lipid Particles 0.0119 0.0003 0.0091 0.0341 0.013 0.0014 0.0129 0.0003 0.0082 0.0189 0.039 0.0003 0.0238 0.0005 0.0007 0.0012 0.0163 0.0035
Mitochondria 0.0058 0.0008 0.0037 0.0014 0.021 0.0008 0.0033 0.0007 0.0069 0.0029 0.0302 0.0147 0.005 0.0026 0.0006 0.0033 0.0036 0.0011
None 0.1026 0.1254 0.1081 0.3258 0.1936 0.0846 0.4144 0.2093 0.1932 0.2154 0.2179 0.1742 0.1856 0.2242 0.2558 0.2358 0.0979 0.0795
Nuclear Periphery 0.0239 0.016 0.0165 0.0115 0.0062 0.0104 0.0478 0.0202 0.0102 0.0094 0.0182 0.0073 0.0378 0.0197 0.0181 0.0119 0.017 0.0095
Nucleolus 0.0058 0.0103 0.0068 0.0054 0.0088 0.0151 0.0035 0.0088 0.012 0.0068 0.0044 0.0125 0.0058 0.0065 0.0088 0.0031 0.0112 0.0097
Nucleus 0.5905 0.7456 0.7653 0.5296 0.6042 0.7916 0.3035 0.65 0.5711 0.4983 0.4671 0.6798 0.5059 0.5934 0.5856 0.6372 0.6427 0.7451
Peroxisomes 0.0223 0.0001 0.0098 0.0152 0.0293 0.0011 0.004 0.0001 0.0029 0.0144 0.0102 0.0001 0.0178 0.0003 0.0002 0.0003 0.0061 0.0036
Punctate Nuclear 0.0319 0.024 0.0194 0.0306 0.0548 0.0337 0.0647 0.0222 0.0146 0.0107 0.0079 0.033 0.0207 0.0146 0.0207 0.0113 0.0183 0.023
Vacuole 0.005 0.0034 0.0009 0.0004 0.0004 0.0014 0.0019 0.0019 0.0077 0.0058 0.0032 0.002 0.0041 0.0049 0.002 0.0012 0.0016 0.004
Vacuole Periphery 0.0025 0.0005 0.0005 0.0002 0.0003 0.0004 0.0027 0.0003 0.0023 0.001 0.0021 0.0002 0.0021 0.0007 0.0003 0.0008 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7647 7.7493 9.0362 16.5219 12.845 19.8033 14.2715 19.6423 20.7034 12.5752
Translational Efficiency 1.1312 1.3555 1.4112 1.0396 0.5703 0.866 1.2964 1.2496 1.2154 1.0308

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1124 1318 2502 1404 2110 2139 238 1779 3234 3457 2740 3183

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 628.14 761.84 863.69 823.49 699.89 786.18 948.47 929.51 674.95 776.90 871.05 882.75
Standard Deviation 94.29 99.50 109.88 122.73 74.78 92.55 104.52 139.98 88.92 95.99 112.00 142.71
Intensity Change Log 2 — 0.278402 0.459427 0.390665 — 0.167731 0.438474 0.409342 — 0.221136 0.448423 0.400538

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000363 0.000189 0.000581 0.000024 0.000256 0.000699 0.000955 0.000034 0.000297 0.000233 0.000790
Bud Neck 0.003623 0.016910 0.008137 0.011887 0.004704 0.012178 0.004921 0.021324 0.004328 0.013982 0.007857 0.017161
Bud Site 0.000423 0.006106 0.001153 0.013725 0.000782 0.005477 0.001072 0.008956 0.000657 0.005717 0.001146 0.011060
Cell Periphery 0.000213 0.000238 0.000081 0.000271 0.000125 0.000167 0.000075 0.000178 0.000156 0.000194 0.000081 0.000219
Cytoplasm 0.236794* 0.258299* 0.014676 0.120652* 0.287518* 0.275835* 0.006324 0.045723 0.269888* 0.269149* 0.013951 0.078774
Cytoplasmic Foci 0.060316 0.075047 0.000199 0.009277 0.028292 0.061058 0.000105 0.001778 0.039422 0.066392 0.000191 0.005086
Eisosomes 0.000073 0.000070 0.000012 0.000038 0.000022 0.000056 0.000034 0.000025 0.000040 0.000061 0.000014 0.000031
Endoplasmic Reticulum 0.000689 0.003091 0.003171 0.004078 0.000550 0.000829 0.009206 0.006653 0.000598 0.001692 0.003695 0.005517
Endosome 0.002247 0.005310 0.000245 0.006592 0.000657 0.003400 0.000315 0.002547 0.001210 0.004128 0.000251 0.004331
Golgi 0.000286 0.001954 0.000025 0.003991 0.000117 0.001105 0.000045 0.002252 0.000176 0.001428 0.000027 0.003019
Lipid Particles 0.008757 0.005410 0.000096 0.001183 0.001344 0.003778 0.000332 0.000410 0.003921 0.004400 0.000116 0.000751
Mitochondria 0.002383 0.008637 0.000941 0.002948 0.001652 0.006729 0.000890 0.003235 0.001906 0.007457 0.000936 0.003108
Mitotic Spindle 0.000327 0.003727 0.002012 0.032638 0.000656 0.006729 0.000626 0.017132 0.000542 0.005585 0.001892 0.023972
None 0.023140 0.006519 0.002622 0.016455 0.004018 0.006198 0.004210 0.007022 0.010664 0.006320 0.002760 0.011183
Nuclear Periphery 0.001601 0.002306 0.000984 0.002186 0.001024 0.001047 0.000736 0.002268 0.001224 0.001527 0.000962 0.002232
Nuclear Periphery Foci 0.000329 0.000691 0.000075 0.000970 0.000147 0.000251 0.000137 0.000420 0.000211 0.000419 0.000080 0.000663
Nucleolus 0.004535 0.002911 0.000914 0.002431 0.000810 0.002348 0.002210 0.001481 0.002105 0.002562 0.001027 0.001900
Nucleus 0.642592* 0.567428* 0.962422* 0.734896* 0.661496* 0.593005* 0.961137* 0.857940* 0.654926* 0.583254* 0.962310* 0.803667*
Peroxisomes 0.002294 0.007245 0.000033 0.001065 0.000715 0.005646 0.000135 0.000797 0.001264 0.006256 0.000042 0.000915
Vacuole 0.008982 0.026520 0.001902 0.030563 0.005225 0.013111 0.006691 0.016793 0.006531 0.018223 0.002318 0.022867
Vacuole Periphery 0.000341 0.001219 0.000111 0.003573 0.000123 0.000797 0.000099 0.002110 0.000199 0.000958 0.000110 0.002755

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.56 -5.72 -9.56 -2.40 -7.41 -2.62 -11.22 -6.46 -4.18 -0.81 -4.20 -15.33 -9.30 -4.87 -6.31
Bud Neck -8.05 -6.54 -8.02 1.99 -3.01 -6.84 -3.05 -12.61 -6.76 -10.48 -10.45 -6.91 -14.79 -4.22 -8.87
Bud Site -6.82 -4.27 -6.64 -2.42 -5.99 -4.98 -2.56 -8.33 -2.10 -7.08 -7.63 -3.37 -10.26 -3.20 -9.24
Cell Periphery -0.83 8.75 0.75 1.29 -6.31 -2.28 2.37 -1.61 0.79 -4.16 -2.34 6.54 -1.20 1.32 -7.87
Cytoplasm -1.98 30.84 16.64 19.45 -17.57 1.62 49.66 42.14 39.62 -12.99 0.34 57.35 41.28 41.24 -19.90
Cytoplasmic Foci -2.59 17.72 15.83 18.36 -6.40 -10.18 15.99 15.26 22.09 -3.32 -9.58 23.49 21.34 28.50 -6.95
Eisosomes 0.51 16.52 6.71 5.43 -17.49 -9.51 -4.43 -5.11 7.04 1.97 -7.12 15.26 1.33 8.50 -18.33
Endoplasmic Reticulum -7.67 -19.45 -19.13 -6.02 -7.54 -2.54 -11.95 -21.14 -20.30 2.14 -7.87 -22.37 -27.79 -19.99 -10.88
Endosome -4.94 7.35 -2.17 1.87 -5.64 -7.38 2.55 -3.48 3.78 -4.43 -8.94 8.31 -4.12 3.51 -7.29
Golgi -2.60 5.73 -2.75 -0.04 -3.20 -4.26 2.32 -2.51 -0.11 -2.65 -4.46 6.65 -3.77 -0.20 -4.20
Lipid Particles 3.37 10.45 9.24 7.74 -6.93 -6.12 7.22 6.71 8.58 -1.70 -1.18 12.39 10.29 11.40 -9.10
Mitochondria -4.67 3.20 0.04 4.85 -5.13 -4.70 1.16 -2.80 3.56 -6.85 -6.56 2.34 -2.37 5.85 -8.49
Mitotic Spindle -2.95 -3.15 -7.95 -6.51 -7.02 -4.74 -0.93 -7.72 -4.47 -7.06 -5.55 -3.10 -11.06 -7.59 -9.47
None 6.20 7.74 4.08 -3.17 -6.17 -3.69 0.53 -2.47 0.34 -2.18 4.16 8.05 2.00 -2.33 -7.38
Nuclear Periphery -1.57 1.55 -4.35 -1.04 -9.81 -0.16 0.77 -11.93 -10.38 -13.66 -1.54 0.34 -11.70 -6.65 -15.47
Nuclear Periphery Foci -3.10 3.55 -4.07 -1.41 -6.23 -1.64 -0.51 -3.32 -2.59 -3.09 -3.47 2.07 -5.38 -3.10 -7.13
Nucleolus 2.92 6.04 2.31 -0.44 -4.79 -6.39 -3.07 -6.60 1.69 0.44 -1.49 4.39 -0.88 0.59 -5.95
Nucleus 5.83 -34.10 -11.35 -17.95 23.71 7.66 -40.08 -24.15 -30.28 15.12 9.75 -56.78 -24.40 -33.60 29.01
Peroxisomes -4.21 4.05 2.89 6.57 -2.54 -6.77 5.16 1.05 6.90 -1.45 -7.98 5.86 2.94 9.50 -3.02
Vacuole -9.51 8.68 -11.18 -2.86 -13.97 -8.12 -2.35 -10.75 -5.82 -5.91 -12.51 7.50 -15.71 -6.44 -18.05
Vacuole Periphery -4.81 3.60 -2.83 -1.14 -3.29 -3.87 0.32 -2.92 -0.96 -2.94 -5.92 2.80 -4.13 -1.56 -4.41
Standard name
Human Ortholog
Description 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (31%), cytoplasm (5%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mag1

Mag1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mag1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available