Standard name
Human Ortholog
Description GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.97 0.96 0.95 – 0.8 0.78 0.8 0.77 0.78 0.97 0.98 0.99 0.84 0.88 0.88 0.87 0.7 0.67 0.79 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.06 0 0.11 – 0.27 0.32 0.2 0.23 0.25 0 0 0 0 0 0 0 0.09 0.09 0 0.06 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0.05 0.06 0 0 0 0.16 0.1 0.11 0 0.1 0.12 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 1 0 2 1 3 7
Bud 0 0 1 5 5 3 – 12 16 17 8 15 1 0 2 0 0 0 0 0 0 0 8 16
Bud Neck 0 0 3 1 13 10 – 11 7 5 2 2 0 0 1 0 1 0 1 2 0 1 0 1
Bud Site 0 0 0 0 0 2 – 0 1 5 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 4 3 3 – 0 4 1 4 5 0 3 1 2 1 6 0 0 0 0 2 2
Cytoplasm 285 92 154 407 652 548 – 533 391 475 294 352 234 712 730 125 125 145 256 90 92 59 372 355
Endoplasmic Reticulum 5 2 0 0 3 0 – 2 2 5 14 8 3 6 1 5 9 10 0 2 1 0 2 2
Endosome 0 0 0 0 1 0 – 0 1 0 1 2 0 0 0 4 6 2 1 2 2 0 2 5
Golgi 0 0 0 1 0 0 – 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 1 2
Mitochondria 6 6 3 27 23 64 – 180 159 119 90 112 3 6 2 5 2 2 11 11 13 2 29 21
Nucleus 0 0 0 1 2 2 – 14 6 13 7 10 1 1 1 1 0 3 0 0 0 0 1 7
Nuclear Periphery 0 0 0 1 0 0 – 9 5 11 5 5 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 3 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 7 4 – 4 5 6 3 7 0 0 0 0 2 1 2 0 1 1 4 11
Vac/Vac Membrane 0 0 0 1 3 4 – 20 11 23 18 27 1 1 2 24 14 18 12 12 16 5 18 24
Unique Cell Count 291 95 155 421 676 574 666 500 591 384 450 240 724 736 149 142 164 294 128 139 75 458 472
Labelled Cell Count 298 100 164 449 713 640 788 609 682 449 547 244 732 742 167 160 187 294 128 139 75 458 472


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.7 3.2 2.9 3.3 2.8 – 3.3 2.9 3.6 3.8 4.0 4.0 4.3 4.2 6.2 6.2 6.4 3.8 4.0 4.5
Std Deviation (1e-4) 0.5 5.2 0.5 0.9 1.1 1.0 – 1.3 1.4 1.7 2.5 2.6 1.1 0.8 1.1 1.9 2.0 1.9 1.3 1.7 2.2
Intensity Change (Log2) – – – -0.17 0.03 -0.23 – 0.01 -0.14 0.15 0.24 0.31 0.32 0.4 0.37 0.93 0.94 0.97 0.25 0.32 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.2 4.0 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6589 0.4068 0.0413 0.6153 0.1565 -0.0192 -0.5753 -0.6405 -0.632 -0.8071 -0.4824 -0.7485 0.34 1.5051 0.9293 1.1073 1.0298 0.8143
Actin 0.0625 0.0002 0.0096 0.0776 0.0463 0.0001 0.0334 0.0027 0.0113 0.0248 0.0115 0.0017 0.0002 0.0004 0.0004 0.0002 0.0005 0.0001
Bud 0.001 0.0005 0.0003 0.0037 0.0098 0.0001 0.0013 0.0045 0.0041 0.0011 0.0007 0.0001 0.0001 0.003 0.0015 0.0002 0.0001 0
Bud Neck 0.0018 0.0001 0.0004 0.0004 0.0005 0.0002 0.0027 0.0003 0.0028 0.0005 0.014 0.0007 0 0.0001 0.0001 0.0001 0.0002 0.0003
Bud Periphery 0.0016 0.0004 0.0003 0.0223 0.0264 0.0002 0.0022 0.0058 0.0039 0.0027 0.0021 0.0002 0.0001 0.003 0.003 0.0007 0.0001 0
Bud Site 0.011 0.0018 0.0023 0.0011 0.0038 0 0.0119 0.0107 0.0054 0.0017 0.0026 0.0005 0.0001 0.0037 0.001 0.0001 0.0001 0.0001
Cell Periphery 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0009 0.0004 0.0004 0.0001 0.0003 0 0.0002 0.0004 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.0841 0.1643 0.1363 0.1406 0.1226 0.2371 0.1098 0.1926 0.1452 0.1477 0.1156 0.2059 0.1504 0.1918 0.1947 0.1421 0.2285 0.2369
Cytoplasmic Foci 0.0233 0.0057 0.0075 0.0289 0.0297 0.0086 0.0351 0.014 0.0125 0.0139 0.0127 0.0091 0.0091 0.0084 0.0096 0.0031 0.0039 0.0042
Eisosomes 0.0004 0 0.0002 0.0003 0.0002 0 0.0005 0.0001 0.0003 0.0002 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.003 0.0222 0.0182 0.0312 0.0013 0.0017 0.0062 0.0047 0.0082 0.0055 0.0016 0.0069 0.0276 0.0236 0.0226 0.0031 0.055 0.0304
Endosome 0.0178 0.0041 0.0078 0.0201 0.0212 0.0048 0.0326 0.002 0.0069 0.0067 0.0339 0.0084 0.0014 0.0028 0.0035 0.0056 0.0006 0.0026
Golgi 0.0115 0.0001 0.0044 0.0093 0.0057 0.0002 0.0071 0.0002 0.0041 0.0023 0.0273 0.0025 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Lipid Particles 0.0268 0.0004 0.0036 0.0149 0.024 0.0004 0.0124 0.0011 0.0041 0.0009 0.0306 0.0059 0.0007 0.0007 0.0005 0.0001 0.0001 0.0005
Mitochondria 0.0199 0.0002 0.0034 0.0043 0.0266 0.0006 0.0116 0.001 0.0174 0.0033 0.0254 0.0034 0.0002 0.0055 0.0005 0.0002 0.0002 0.0002
None 0.6764 0.7941 0.7914 0.5893 0.6231 0.7418 0.6901 0.7525 0.7578 0.78 0.7003 0.7384 0.8026 0.7437 0.7445 0.8293 0.708 0.7097
Nuclear Periphery 0.0118 0.001 0.0019 0.0291 0.001 0.0004 0.0059 0.0012 0.0015 0.0014 0.0038 0.0037 0.003 0.0023 0.0024 0.0005 0.0009 0.0008
Nucleolus 0.0006 0.0002 0.0001 0.0004 0.0009 0.0001 0.0026 0.0005 0.0008 0.0003 0.0014 0.0001 0.0002 0.0009 0.0003 0.0001 0 0
Nucleus 0.0073 0.0009 0.0006 0.0063 0.0013 0.0006 0.004 0.0016 0.0017 0.0013 0.0012 0.0008 0.001 0.0022 0.0023 0.0005 0.0007 0.0007
Peroxisomes 0.0325 0.0001 0.0009 0.0114 0.0149 0.0001 0.0106 0.0004 0.0063 0.0017 0.0049 0.0065 0.0001 0.0003 0.0001 0 0.0002 0
Punctate Nuclear 0.0042 0.0006 0.0006 0.0045 0.0012 0.0008 0.012 0.0017 0.0029 0.0021 0.0025 0.0013 0.0015 0.0024 0.0016 0.0001 0.0005 0.0002
Vacuole 0.0015 0.0027 0.0096 0.0026 0.0349 0.0017 0.005 0.0018 0.0018 0.0018 0.003 0.0028 0.001 0.0037 0.0102 0.0129 0.0002 0.0127
Vacuole Periphery 0.0008 0.0002 0.0006 0.0015 0.0041 0.0004 0.002 0.0003 0.0006 0.0004 0.0043 0.0008 0.0002 0.0006 0.0007 0.0006 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.4054 2.2067 3.2749 10.3123 6.4009 32.0723 2.2311 8.2823 9.8257 9.1865
Translational Efficiency 3.2378 1.3105 1.8513 0.7807 0.4359 1.9902 5.0072 1.5394 2.3004 0.9611

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1306 1554 503 816 1349 1407 1868 909 2655 2961 2371 1725

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.36 626.89 908.58 829.88 590.36 665.57 809.46 866.62 590.85 645.27 830.49 849.24
Standard Deviation 91.65 95.66 136.30 184.56 197.43 96.97 106.78 181.21 154.72 98.20 120.69 183.72
Intensity Change Log 2 — 0.084176 0.619577 0.488866 — 0.172995 0.455365 0.553805 — 0.129231 0.539875 0.521673

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000075 0.000232 0.000492 0.002892 0.000089 0.000371 0.000564 0.003170 0.000082 0.000298 0.000549 0.003038
Bud Neck 0.005631 0.039508 0.002949 0.010022 0.014115 0.055200 0.015669 0.011551 0.009942 0.046965 0.012971 0.010828
Bud Site 0.001517 0.012796 0.005228 0.051171 0.004852 0.015745 0.017516 0.048955 0.003211 0.014197 0.014909 0.050003
Cell Periphery 0.000226 0.000087 0.000315 0.004419 0.000259 0.000157 0.000162 0.001057 0.000243 0.000120 0.000195 0.002648
Cytoplasm 0.562887* 0.449159* 0.282249* 0.442735* 0.451496* 0.377277* 0.407321* 0.468109* 0.506289* 0.415003* 0.380787* 0.456106*
Cytoplasmic Foci 0.165099 0.225699* 0.021934 0.030824 0.215787* 0.256227* 0.008276 0.037791 0.190853* 0.240205* 0.011173 0.034495
Eisosomes 0.000338 0.000108 0.000073 0.000329 0.000184 0.000204 0.000032 0.000133 0.000260 0.000154 0.000041 0.000225
Endoplasmic Reticulum 0.001015 0.000463 0.067886 0.014314 0.000514 0.000839 0.007168 0.009230 0.000760 0.000642 0.020049 0.011635
Endosome 0.004706 0.021891 0.006808 0.010451 0.009031 0.033329 0.002373 0.013871 0.006904 0.027326 0.003314 0.012253
Golgi 0.000998 0.003263 0.000136 0.011144 0.001190 0.006016 0.000382 0.010445 0.001095 0.004571 0.000330 0.010775
Lipid Particles 0.007815 0.007945 0.005421 0.008368 0.014315 0.009708 0.001153 0.007246 0.011117 0.008783 0.002058 0.007776
Mitochondria 0.003078 0.010301 0.000488 0.007051 0.005372 0.017014 0.001666 0.007800 0.004244 0.013491 0.001416 0.007446
Mitotic Spindle 0.000730 0.003075 0.000265 0.039874 0.000639 0.004989 0.016209 0.016543 0.000684 0.003984 0.012827 0.027580
None 0.023146 0.006123 0.002143 0.007282 0.008428 0.003437 0.003797 0.003344 0.015667 0.004846 0.003446 0.005207
Nuclear Periphery 0.000628 0.000546 0.016679 0.001398 0.001160 0.000400 0.005108 0.000909 0.000898 0.000476 0.007563 0.001140
Nuclear Periphery Foci 0.000906 0.000403 0.027275 0.003846 0.000317 0.000248 0.001517 0.003479 0.000607 0.000329 0.006981 0.003652
Nucleolus 0.001889 0.003673 0.000586 0.000307 0.002002 0.002838 0.000414 0.000255 0.001947 0.003276 0.000451 0.000280
Nucleus 0.194030* 0.119741 0.466464* 0.181674* 0.220537* 0.107592 0.454601* 0.143414* 0.207499* 0.113968 0.457118* 0.161513*
Peroxisomes 0.003357 0.016609 0.000341 0.001767 0.005972 0.024769 0.002291 0.003214 0.004686 0.020487 0.001877 0.002529
Vacuole 0.021178 0.076501 0.092023 0.166369 0.043064 0.080738 0.053376 0.202481* 0.032298 0.078514 0.061575 0.185398
Vacuole Periphery 0.000752 0.001876 0.000246 0.003765 0.000678 0.002903 0.000405 0.007006 0.000714 0.002364 0.000371 0.005473

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.58 -8.25 -4.62 -4.14 -3.15 -6.59 -2.19 -3.77 -3.08 -2.77 -8.58 -3.05 -5.76 -4.96 -4.00
Bud Neck -14.01 4.22 -2.51 12.99 -6.30 -13.03 0.90 3.87 15.26 2.62 -18.69 -0.42 2.40 19.88 2.16
Bud Site -9.65 -1.79 -7.50 -4.15 -5.63 -5.64 -4.12 -6.46 -3.52 -4.00 -9.70 -5.61 -9.83 -5.46 -5.93
Cell Periphery 5.78 -2.60 -1.83 -2.00 -1.72 1.40 1.51 -1.57 -2.37 -2.42 3.16 1.49 -2.14 -2.46 -2.30
Cytoplasm 11.66 23.26 16.25 6.42 -6.49 7.10 10.42 5.15 -0.91 -2.94 12.66 23.12 14.79 3.97 -4.69
Cytoplasmic Foci -8.44 27.56 27.66 36.83 -0.35 -5.00 38.81 33.53 37.60 -11.56 -9.13 48.58 43.04 52.41 -11.44
Eisosomes 3.00 3.34 1.75 -1.36 -1.87 -1.16 17.69 5.71 5.66 -5.33 2.79 5.59 2.69 0.57 -3.61
Endoplasmic Reticulum 2.80 -8.37 -4.20 -4.57 7.16 -1.27 -6.60 -3.13 -2.88 0.52 0.76 -9.93 -5.16 -5.26 5.35
Endosome -13.28 -2.88 -0.33 11.36 1.61 -11.73 8.53 1.61 11.91 -4.20 -16.96 7.65 1.09 16.08 -4.00
Golgi -7.24 6.43 -1.85 -0.41 -2.43 -6.61 4.88 -2.12 1.26 -2.87 -9.00 6.86 -2.81 0.52 -3.71
Lipid Particles -0.15 3.39 1.68 1.84 -0.67 3.21 10.64 6.24 3.96 -3.28 2.75 12.62 6.05 4.18 -4.35
Mitochondria -4.95 2.88 0.19 5.35 -2.97 -5.98 4.12 1.24 6.46 -1.65 -7.68 4.54 1.09 8.21 -2.58
Mitotic Spindle -2.77 0.66 -6.73 -6.04 -6.90 -3.67 -6.71 -5.89 -3.63 -0.28 -4.62 -6.57 -8.86 -7.07 -3.56
None 6.08 7.80 6.60 1.33 -2.42 3.47 3.35 3.84 0.65 1.99 6.90 8.08 7.63 1.63 -0.32
Nuclear Periphery 0.48 -6.04 -7.96 -9.96 5.22 2.21 -4.59 -1.35 -12.37 4.42 2.19 -7.83 -5.26 -14.70 6.44
Nuclear Periphery Foci 2.60 -7.72 -5.56 -7.26 6.84 1.21 -5.53 -5.74 -5.96 -3.00 2.78 -8.20 -7.93 -9.16 4.12
Nucleolus -4.64 5.85 7.35 9.65 3.92 -1.46 6.17 6.63 5.64 1.81 -4.12 8.74 9.73 10.62 2.96
Nucleus 11.64 -20.58 -1.20 -9.75 18.15 16.44 -31.32 4.79 -8.34 31.83 19.92 -40.05 2.63 -12.65 37.34
Peroxisomes -8.66 3.64 2.96 12.10 -3.11 -10.36 5.18 4.24 12.73 -0.06 -13.40 5.20 5.09 17.21 -0.03
Vacuole -17.85 -14.05 -27.44 -21.12 -16.05 -9.99 -10.95 -26.60 -22.14 -21.13 -18.94 -17.94 -38.18 -30.64 -27.45
Vacuole Periphery -3.61 1.94 -1.25 1.11 -2.74 -5.15 4.03 -2.30 0.06 -2.75 -6.39 3.08 -2.61 0.37 -3.54
Standard name
Human Ortholog
Description GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Spi1

Spi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available