Standard name
Human Ortholog
Description Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0.07 0.08 0 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0.07 0.11 0.08 0.08 0.08 0.05 0.06 0 0 0.08 0.11 0 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.1 – 0.05 0 0.12 0.24 0.22 0.41 0.31 0.46 0.23 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.9 – 0.83 0.9 0.83 0.79 0.78 0.74 0.77 0.72 0.81 0.84 0.84 0.83 0.85 0.85 0.79 0.9 0.83 0.86 0.77 0.65 0.55
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.35 – 0.23 0 0.11 0.13 0.17 0.16 0.22 0.19 0.34 0.31 0.05 0 0 0 0.05 0.05 0 0.1 0.05 0.11 0.15
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.06 0 0 0 0.06 0.15 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 2 4 1 1 0 0 0 0 0 0 0 0 1 2 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 1 0 3 4 3 12 13 2 9 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 0 – 0 9 13 16 14 14 8 8 2 1 10 16 5 6 8 0 1 0 0 3 2
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 2 0 0 1 1 2
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 3
Mitochondria 31 2 – 3 1 15 46 39 75 53 59 27 3 0 0 5 0 4 2 0 0 1 1 5
Nucleus 274 18 – 55 110 100 154 135 134 131 91 94 43 103 124 116 90 86 270 46 41 30 75 68
Nuclear Periphery 2 0 – 0 0 2 0 3 2 1 3 2 0 1 1 2 1 1 0 0 0 0 0 1
Nucleolus 47 7 – 15 3 13 25 30 30 38 24 39 16 6 5 4 1 5 14 1 4 1 12 19
Peroxisomes 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 3 0 2 1 0 0 1 1 1 0 0 0 0 0 0 1 1
Vac/Vac Membrane 2 0 – 0 0 1 2 0 2 1 1 1 0 3 6 12 7 7 4 1 0 2 17 11
Unique Cell Count 297 20 66 122 121 195 174 182 171 127 116 51 122 150 137 106 109 299 56 49 40 117 125
Labelled Cell Count 360 27 74 123 147 251 231 270 248 189 174 63 124 154 149 109 115 299 56 49 40 117 125


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 8.1 – 5.9 7.2 5.7 5.4 5.6 5.0 4.9 4.7 4.9 6.5 8.6 8.4 9.3 10.5 10.2 – 8.4 8.9
Std Deviation (1e-4) 0.9 1.5 – 0.9 1.6 1.4 1.8 1.8 1.6 1.4 1.5 1.0 1.3 2.1 2.3 2.3 2.3 2.3 – 1.8 2.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6594 3.5855 2.955 3.2476 2.7774 2.9352 3.1694 3.5635 3.0656 2.5934 1.794 3.113 2.5279 3.7102 3.0734 2.8218 1.7041 2.5375
Actin 0.019 0.0081 0.0122 0.0014 0.0007 0 0.1061 0.002 0.0043 0.0025 0.0001 0.0126 0.0618 0.0054 0.0225 0.0007 0.0029 0.0007
Bud 0.0008 0.0008 0.0003 0.0001 0.0002 0 0.0009 0.0002 0.0002 0 0.0003 0.0003 0.0003 0.0002 0.0002 0.0001 0.0015 0
Bud Neck 0.0136 0.0002 0.0007 0.0017 0.0027 0.0012 0.0173 0.0003 0.0002 0.0003 0.0008 0.0026 0.0041 0.0002 0.0007 0.0004 0.0009 0.0012
Bud Periphery 0.0015 0.0013 0.0004 0.0002 0.0003 0.0001 0.0013 0.0005 0.0002 0 0.0008 0.0005 0.0004 0.0002 0.0002 0.0002 0.0029 0.0001
Bud Site 0.0106 0.0012 0.0046 0.0008 0.0002 0 0.0118 0.0036 0.0004 0.0004 0.0008 0.0023 0.0026 0.0029 0.0039 0 0.0037 0.0001
Cell Periphery 0.0011 0.0001 0.0003 0.0004 0.0001 0 0.0007 0.0008 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0002 0
Cytoplasm 0.0128 0.0017 0.0048 0.0027 0.091 0 0.0111 0.0089 0.001 0.0522 0 0.0001 0.0162 0.0043 0.0001 0.001 0.0091 0
Cytoplasmic Foci 0.027 0.0051 0.0073 0.0002 0.0008 0 0.0638 0.0019 0.0082 0.0125 0 0.0028 0.0482 0.004 0.0047 0.0008 0.048 0.0004
Eisosomes 0.0016 0 0.0002 0.0001 0 0 0.0015 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0179 0.0004 0.0014 0.0022 0.0008 0 0.0087 0.0033 0.0014 0.0017 0 0.0001 0.0162 0.0004 0.0001 0.0008 0.0055 0
Endosome 0.0497 0.0016 0.0196 0.001 0.0005 0 0.0923 0.0034 0.0133 0.0054 0.0001 0.0019 0.0608 0.0013 0.0036 0.0031 0.0719 0.0008
Golgi 0.0185 0.0017 0.0049 0.0001 0.0001 0 0.039 0.0013 0.0115 0.0005 0 0.0034 0.012 0.001 0.0076 0.0003 0.0102 0.0013
Lipid Particles 0.0297 0.0011 0.005 0.0005 0.0001 0 0.0534 0.0004 0.0019 0.0004 0.0001 0.0036 0.0267 0.004 0.0039 0.0005 0.0282 0.012
Mitochondria 0.0067 0.0015 0.0118 0.0006 0.0012 0.0001 0.0086 0.0011 0.0033 0.0004 0.0009 0.0046 0.0046 0.0028 0.0033 0.0002 0.0406 0.0019
None 0.0402 0.0005 0.0151 0.0009 0.0017 0 0.013 0.0051 0.0002 0.0123 0.0001 0.0001 0.0109 0.0003 0.0001 0.0002 0.006 0
Nuclear Periphery 0.0628 0.0052 0.0344 0.0213 0.0062 0.0005 0.0403 0.0016 0.0182 0.0103 0.0005 0.0022 0.0404 0.0049 0.0015 0.0459 0.0219 0.0065
Nucleolus 0.0288 0.0181 0.016 0.0266 0.1419 0.109 0.0168 0.024 0.0407 0.0095 0.3476 0.1174 0.0161 0.0212 0.0191 0.0201 0.1038 0.1033
Nucleus 0.6047 0.9336 0.8364 0.9292 0.7487 0.8881 0.4479 0.9388 0.8861 0.8386 0.6446 0.8385 0.6009 0.943 0.9238 0.9155 0.6016 0.8695
Peroxisomes 0.0191 0.002 0.0016 0.0001 0 0 0.0218 0.0001 0.0059 0.0008 0.0001 0.0051 0.0333 0.0004 0.0035 0.0001 0.0082 0.0009
Punctate Nuclear 0.018 0.0147 0.0181 0.0078 0.0015 0.0004 0.0371 0.0008 0.0015 0.0521 0.0028 0.0009 0.0396 0.002 0.0007 0.0084 0.0229 0.0008
Vacuole 0.0094 0.0007 0.0034 0.0015 0.0006 0.0003 0.0038 0.0017 0.0006 0.0002 0.0003 0.0005 0.0026 0.0005 0.0002 0.0007 0.0064 0.0001
Vacuole Periphery 0.0062 0.0003 0.0015 0.0007 0.0007 0.0001 0.0026 0.0002 0.0007 0.0001 0.0001 0.0003 0.0018 0.0008 0.0001 0.0009 0.0036 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6072 12.7973 19.7228 17.9564 17.847 12.1065 14.93 19.1458 23.3192 18.8516
Translational Efficiency 3.551 1.8766 1.1078 1.6459 1.2284 1.9502 1.5948 1.307 1.0936 1.1051

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1820 265 1658 1404 1779 232 264 1260 3599 497 1922 2664

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 949.18 1013.37 1022.84 1068.96 901.33 995.20 1156.31 1139.34 925.53 1004.89 1041.17 1102.25
Standard Deviation 133.64 170.90 158.80 164.91 132.25 148.78 132.00 179.57 135.09 161.21 162.04 175.55
Intensity Change Log 2 — 0.094407 0.107827 0.171454 — 0.142931 0.359401 0.338071 — 0.118246 0.235843 0.255013

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000327 0.000939 0.000724 0.001220 0.000384 0.000867 0.001580 0.001480 0.000355 0.000906 0.000842 0.001343
Bud Neck 0.004984 0.010386 0.023632 0.030645 0.007868 0.012226 0.009074 0.037664 0.006409 0.011245 0.021633 0.033965
Bud Site 0.000423 0.005383 0.001428 0.004716 0.000808 0.002245 0.001255 0.015730 0.000613 0.003918 0.001404 0.009925
Cell Periphery 0.000031 0.000062 0.000047 0.000102 0.000044 0.000036 0.000040 0.000243 0.000037 0.000050 0.000046 0.000168
Cytoplasm 0.000873 0.002338 0.000568 0.004112 0.000570 0.000349 0.000187 0.007975 0.000723 0.001409 0.000515 0.005939
Cytoplasmic Foci 0.000306 0.001393 0.000015 0.001083 0.000134 0.002834 0.000070 0.008699 0.000221 0.002066 0.000023 0.004685
Eisosomes 0.000032 0.000041 0.000039 0.000037 0.000036 0.000037 0.000066 0.000037 0.000034 0.000039 0.000043 0.000037
Endoplasmic Reticulum 0.001975 0.002484 0.001733 0.003458 0.002074 0.002479 0.004779 0.004252 0.002024 0.002481 0.002152 0.003834
Endosome 0.000165 0.000749 0.000090 0.002049 0.000171 0.001326 0.000112 0.008994 0.000168 0.001018 0.000093 0.005334
Golgi 0.000112 0.000331 0.000101 0.003182 0.000111 0.004382 0.000155 0.013093 0.000112 0.002222 0.000109 0.007870
Lipid Particles 0.000059 0.000119 0.000026 0.000171 0.000039 0.002025 0.000126 0.000253 0.000049 0.001009 0.000040 0.000210
Mitochondria 0.000794 0.001039 0.001132 0.004268 0.000657 0.002823 0.001369 0.009347 0.000727 0.001871 0.001165 0.006670
Mitotic Spindle 0.001665 0.011555 0.001155 0.024661 0.000975 0.020398 0.001204 0.064949 0.001324 0.015683 0.001162 0.043716
None 0.001576 0.002765 0.001494 0.004244 0.001173 0.000990 0.001977 0.005448 0.001377 0.001937 0.001561 0.004813
Nuclear Periphery 0.000108 0.000108 0.000181 0.001518 0.000167 0.000219 0.000162 0.001484 0.000137 0.000160 0.000178 0.001502
Nuclear Periphery Foci 0.000317 0.000416 0.000058 0.001058 0.000184 0.000314 0.000122 0.000834 0.000251 0.000368 0.000067 0.000952
Nucleolus 0.095169 0.111833 0.039760 0.028734 0.081711 0.113450 0.047660 0.018823 0.088517 0.112588 0.040845 0.024046
Nucleus 0.890046* 0.845267* 0.926952* 0.873378* 0.901777* 0.830168* 0.926832* 0.783941* 0.895844* 0.838219* 0.926936* 0.831077*
Peroxisomes 0.000486 0.001803 0.000446 0.000440 0.000447 0.001700 0.001904 0.002180 0.000467 0.001755 0.000646 0.001263
Vacuole 0.000294 0.000763 0.000273 0.006322 0.000318 0.000677 0.001167 0.009905 0.000306 0.000723 0.000396 0.008017
Vacuole Periphery 0.000259 0.000226 0.000143 0.004602 0.000353 0.000456 0.000160 0.004668 0.000305 0.000333 0.000145 0.004633

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.66 -19.21 -23.90 -5.42 -12.94 -2.98 -12.93 -16.51 -5.79 -1.31 -6.16 -21.23 -26.31 -7.82 -13.51
Bud Neck -3.89 -21.44 -19.27 -11.65 -5.41 -1.61 -4.13 -18.00 -7.76 -12.77 -3.13 -20.37 -26.16 -12.80 -10.22
Bud Site -1.64 -9.02 -7.68 0.27 -5.32 -1.54 -3.37 -7.64 -5.07 -6.88 -2.02 -6.62 -10.09 -1.93 -8.57
Cell Periphery -1.91 -3.28 -9.06 -2.03 -6.92 0.41 -0.62 -5.66 -5.77 -5.65 -1.35 -2.32 -8.33 -5.71 -7.50
Cytoplasm -0.97 1.11 -3.22 -0.70 -4.17 0.45 1.45 -5.37 -5.43 -5.60 -0.90 1.11 -6.27 -3.79 -6.79
Cytoplasmic Foci -1.01 1.39 -0.20 0.97 -3.05 -1.67 0.26 -3.53 -0.08 -3.58 -1.96 1.63 -3.28 0.44 -4.28
Eisosomes -3.11 -6.10 -8.02 -0.90 -1.70 -0.37 -9.09 -7.11 -3.52 5.25 -2.46 -8.23 -10.56 -3.12 -0.93
Endoplasmic Reticulum -2.08 0.67 -10.94 -5.37 -12.50 -0.90 -7.65 -10.12 -3.88 -0.73 -1.72 -2.71 -14.51 -5.86 -12.62
Endosome -1.27 1.26 -3.67 -0.70 -4.01 -1.94 0.66 -3.76 -1.93 -3.80 -2.29 1.94 -4.78 -2.03 -4.95
Golgi -1.93 -0.32 -2.21 -1.81 -2.20 -1.44 -2.85 -3.39 -0.06 -3.33 -1.53 -1.01 -4.04 -0.49 -4.01
Lipid Particles -1.03 1.06 -2.17 -1.25 -4.26 -1.15 -4.97 -7.96 0.99 -4.69 -1.19 0.49 -5.88 0.95 -7.97
Mitochondria -1.03 -2.11 -7.65 -8.21 -7.85 -1.99 -5.05 -7.66 -2.62 -6.32 -2.20 -4.43 -10.54 -4.84 -9.47
Mitotic Spindle -1.72 0.53 -6.07 -1.11 -6.46 -2.39 -0.88 -8.78 -1.93 -8.35 -2.94 -0.11 -10.64 -2.21 -10.68
None -1.32 0.10 -3.73 -1.59 -4.59 0.50 -2.08 -5.19 -5.29 -3.41 -1.17 -1.15 -6.36 -4.40 -6.28
Nuclear Periphery -0.11 -4.37 -7.85 -7.79 -7.21 -1.00 -1.40 -11.76 -10.25 -11.21 -0.90 -3.12 -13.23 -12.47 -12.62
Nuclear Periphery Foci -0.42 1.33 -1.25 -0.85 -2.98 -0.66 1.02 -2.85 -2.24 -3.59 -0.68 1.87 -2.64 -1.96 -4.76
Nucleolus -2.07 13.72 17.76 10.38 4.71 -3.39 5.50 16.66 9.76 4.36 -3.93 15.07 24.23 14.26 7.06
Nucleus 4.02 -6.55 2.35 -2.62 7.24 4.97 -2.17 9.91 0.27 9.71 6.43 -6.49 8.99 -1.49 13.14
Peroxisomes -3.34 0.21 0.39 3.85 0.73 -4.10 -3.31 -2.25 1.42 1.80 -5.11 -2.09 -1.71 3.69 -0.22
Vacuole -1.26 -0.33 -4.95 -4.27 -4.95 -1.54 -4.67 -7.44 -6.80 -6.55 -1.95 -1.76 -8.83 -7.89 -8.67
Vacuole Periphery 0.32 1.26 -2.83 -2.88 -3.00 -0.35 1.64 -2.41 -2.27 -2.88 -0.13 2.15 -3.70 -3.67 -4.14
Standard name
Human Ortholog
Description Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt2

Spt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available