Standard name | |
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Human Ortholog | |
Description | Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 4 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 6 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 3 | 5 | 3 | 3 | 4 | 9 | 12 | 9 | 2 | 11 | 0 | 1 | 0 | 4 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 396 | 383 | 383 | 222 | 157 | 275 | 321 | 270 | 263 | 254 | 161 | 210 | 217 | 305 | 272 | 326 | 289 | 279 | 389 | 372 | 375 | 129 | 257 | 318 |
Endoplasmic Reticulum | 0 | 4 | 2 | 2 | 2 | 7 | 9 | 3 | 11 | 9 | 0 | 2 | 0 | 2 | 2 | 10 | 15 | 11 | 0 | 1 | 1 | 1 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 6 | 4 | 8 | 12 | 54 | 24 | 28 | 60 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | 2 | 2 | 1 | 1 | 4 |
Nucleus | 2 | 1 | 1 | 3 | 3 | 6 | 11 | 3 | 3 | 10 | 6 | 11 | 0 | 2 | 1 | 3 | 7 | 3 | 0 | 5 | 3 | 4 | 7 | 6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 5 | 3 | 18 | 5 | 26 | 23 | 59 | 77 | 32 | 21 | 25 | 35 | 7 | 6 | 0 | 18 | 20 | 46 | 0 | 3 | 4 | 2 | 3 | 4 |
Unique Cell Count | 399 | 391 | 396 | 231 | 185 | 308 | 398 | 359 | 349 | 306 | 206 | 296 | 222 | 310 | 277 | 345 | 308 | 306 | 396 | 390 | 402 | 145 | 288 | 360 |
Labelled Cell Count | 407 | 391 | 407 | 239 | 199 | 318 | 414 | 382 | 381 | 329 | 224 | 334 | 225 | 316 | 277 | 364 | 343 | 349 | 396 | 390 | 402 | 145 | 288 | 360 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.5 | 12.9 | 14.5 | 12.9 | 9.4 | 10.6 | 9.8 | 9.9 | 8.1 | 8.7 | 8.8 | 7.6 | 13.7 | 13.9 | 12.8 | 13.0 | 16.3 | 16.3 | 12.3 | 13.7 | 14.6 |
Std Deviation (1e-4) | 3.0 | 2.6 | 2.4 | 2.1 | 2.2 | 2.0 | 1.8 | 1.9 | 1.6 | 2.0 | 2.4 | 2.1 | 2.7 | 2.2 | 2.4 | 2.9 | 3.5 | 3.6 | 3.7 | 3.9 | 3.9 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.62 | -0.45 | -0.57 | -0.55 | -0.84 | -0.74 | -0.72 | -0.93 | -0.08 | -0.06 | -0.18 | -0.16 | 0.17 | 0.17 | -0.23 | -0.08 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 1.9 | 2.6 | 2.2 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -5.2 | -4.0 | -7.1 | -8.6 | -8.6 | -6.2 | -7.3 | -9.6 | 0.7 | 1.4 | 1.2 | -1.5 | -1.8 | -3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.1 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.7 | 3.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.7 | 8.1 | 5.7 | 7.5 | 9.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | 4.0 | 1.6 | 4.9 | 7.0 | 2.5 | 1.3 | 3.4 | 3.6 | -0.8 | -1.9 | -3.6 | 0.4 | 1.1 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.0978 | 14.2003 | 12.8804 | 11.8688 | 10.2773 | 12.3766 | 10.0012 | 15.4133 | 14.6744 | 13.3685 | 11.764 | 14.7 | 10.359 | 13.6292 | 12.0052 | 12.1335 | 10.4216 | 12.2487 |
Actin | 0.0453 | 0.0002 | 0.0043 | 0.0004 | 0.0036 | 0.0019 | 0.0761 | 0.0006 | 0.0017 | 0.0005 | 0.0638 | 0.0003 | 0.0165 | 0.0003 | 0.0102 | 0.0003 | 0.0111 | 0.0109 |
Bud | 0.0006 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0034 | 0.0001 | 0.0001 | 0.0001 | 0.0072 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0009 | 0.0006 |
Bud Neck | 0.0028 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0011 | 0.0015 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0005 | 0.0014 | 0.0002 | 0.0005 | 0.0005 | 0.0015 | 0.001 |
Bud Periphery | 0.0009 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0033 | 0 | 0 | 0 | 0.0166 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0012 | 0.0004 |
Bud Site | 0.0032 | 0.0002 | 0.0041 | 0.0003 | 0.0004 | 0.0002 | 0.0089 | 0.0001 | 0.0001 | 0.0001 | 0.0069 | 0 | 0.0025 | 0.0002 | 0.001 | 0 | 0.001 | 0.0002 |
Cell Periphery | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.7632 | 0.987 | 0.9337 | 0.8803 | 0.694 | 0.9334 | 0.5832 | 0.9925 | 0.9804 | 0.9815 | 0.6557 | 0.9548 | 0.7824 | 0.9798 | 0.9244 | 0.9636 | 0.8652 | 0.94 |
Cytoplasmic Foci | 0.0262 | 0.0008 | 0.0076 | 0.0041 | 0.0572 | 0.0052 | 0.0713 | 0.0008 | 0.0047 | 0.0015 | 0.0166 | 0.0019 | 0.0328 | 0.0013 | 0.0129 | 0.002 | 0.022 | 0.0077 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.008 | 0.0007 | 0.0023 | 0.0012 | 0.0028 | 0.0016 | 0.0032 | 0.0007 | 0.0005 | 0.0005 | 0.0029 | 0.0013 | 0.0137 | 0.0005 | 0.0015 | 0.0007 | 0.0023 | 0.0012 |
Endosome | 0.0323 | 0.0002 | 0.0112 | 0.0676 | 0.1042 | 0.012 | 0.0567 | 0.0001 | 0.0004 | 0.0003 | 0.0523 | 0.0142 | 0.0553 | 0.0003 | 0.0076 | 0.0004 | 0.013 | 0.002 |
Golgi | 0.012 | 0 | 0.0023 | 0.0131 | 0.0426 | 0.011 | 0.0484 | 0 | 0.0002 | 0 | 0.0075 | 0.0062 | 0.0075 | 0 | 0.0016 | 0 | 0.016 | 0.0005 |
Lipid Particles | 0.0087 | 0 | 0.0061 | 0.0061 | 0.0221 | 0.0039 | 0.0446 | 0 | 0 | 0 | 0.0022 | 0 | 0.0018 | 0 | 0.002 | 0 | 0.0011 | 0.0003 |
Mitochondria | 0.0122 | 0.0001 | 0.0065 | 0.0088 | 0.0459 | 0.0068 | 0.0417 | 0 | 0.0002 | 0.0001 | 0.1159 | 0.0032 | 0.0078 | 0.0001 | 0.0012 | 0.0001 | 0.0364 | 0.0003 |
None | 0.0124 | 0.0013 | 0.0016 | 0.0006 | 0.0008 | 0.0012 | 0.0033 | 0.0014 | 0.001 | 0.0018 | 0.0026 | 0.0012 | 0.0074 | 0.0049 | 0.0019 | 0.0019 | 0.0026 | 0.0022 |
Nuclear Periphery | 0.0133 | 0.0009 | 0.003 | 0.0012 | 0.0027 | 0.0019 | 0.002 | 0.0005 | 0.0007 | 0.0014 | 0.0071 | 0.0012 | 0.0111 | 0.0009 | 0.0054 | 0.0016 | 0.0019 | 0.0015 |
Nucleolus | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0263 | 0.008 | 0.0097 | 0.0063 | 0.0121 | 0.0155 | 0.0033 | 0.0027 | 0.0079 | 0.0111 | 0.0273 | 0.0133 | 0.0369 | 0.0108 | 0.0202 | 0.0275 | 0.0158 | 0.0288 |
Peroxisomes | 0.0204 | 0 | 0.0035 | 0.0005 | 0.0061 | 0.0028 | 0.0396 | 0 | 0.0013 | 0 | 0.0051 | 0 | 0.007 | 0 | 0.0043 | 0.0001 | 0.0051 | 0.0007 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0013 | 0.0002 | 0.001 | 0.0005 | 0.0043 | 0.0001 | 0.0004 | 0.0006 | 0.0036 | 0.0005 | 0.0039 | 0.0002 | 0.0038 | 0.0008 | 0.0011 | 0.0014 |
Vacuole | 0.0057 | 0.0002 | 0.0007 | 0.0055 | 0.002 | 0.0004 | 0.0026 | 0.0001 | 0.0001 | 0.0002 | 0.0033 | 0.0005 | 0.0065 | 0.0002 | 0.0005 | 0.0002 | 0.001 | 0.0002 |
Vacuole Periphery | 0.0023 | 0 | 0.0004 | 0.0028 | 0.0013 | 0.0002 | 0.0017 | 0 | 0 | 0.0001 | 0.0016 | 0.0005 | 0.0031 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.6584 | 18.1403 | 15.9848 | 24.0422 | 26.3101 | 25.3516 | 28.0856 | 29.4009 | 29.8863 | 24.9484 |
Translational Efficiency | 1.8913 | 2.199 | 2.3671 | 1.6897 | 1.3022 | 1.3648 | 1.6243 | 1.5231 | 1.3628 | 1.5208 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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