Standard name
Human Ortholog
Description Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.93 0.96 0.96 0.84 0.79 0.73 0.59 0.64 0.49 0.49 1.0 1.0 0.97 0.98 0.96 0.9 0.77 0.74 0.63 0.89 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.05 0.05 0.2 0.08 0.28 0.4 0.4 0.52 0.6 0.71 0.72 0 0 0 0 0 0 0.13 0.13 0.21 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0
Bud 0 0 0 0 0 0 3 3 3 3 5 5 0 0 0 0 0 0 0 0 0 0 4 1
Bud Neck 0 1 0 0 0 0 0 1 1 1 0 0 1 0 1 0 0 1 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 2 1 0 1 0 1 0 0 1 0 5 7 3 1 0 0 0 0 1
Cytoplasm 106 41 97 93 113 51 109 122 58 89 67 82 87 226 169 163 126 74 87 61 68 43 98 135
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 11 4 0 0 1 0 0 0
Endosome 0 0 2 0 1 0 0 0 0 0 0 0 0 0 1 1 0 4 0 0 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 1 0 0 0
Mitochondria 23 2 5 19 9 17 55 66 51 83 97 120 0 0 1 0 3 3 15 10 23 0 4 5
Nucleus 1 0 0 1 2 1 1 2 1 1 0 0 0 0 0 1 1 0 0 0 0 1 0 1
Nuclear Periphery 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 3 4 1 8 7 0 0 1 0 0 0 0 1 1 0 0 2
Vac/Vac Membrane 0 0 1 0 1 0 1 3 2 1 1 2 0 0 1 3 1 4 2 3 1 0 1 1
Unique Cell Count 114 43 104 97 118 61 138 166 99 139 136 166 87 226 174 167 131 82 114 83 109 49 115 159
Labelled Cell Count 130 44 106 113 126 71 172 202 122 180 180 216 88 227 176 174 152 93 114 83 109 49 115 159


(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.7 3.8 4.1 4.0 3.2 2.9 3.1 2.8 2.8 2.5 2.7 5.0 5.2 5.2 5.4 5.3 4.9 4.8 6.1 5.9
Std Deviation (1e-4) 0.4 0.5 1.8 2.9 2.2 1.7 1.3 1.8 1.1 1.5 1.1 1.5 1.7 1.6 1.9 1.7 1.8 1.3 2.1 2.7 2.4
Intensity Change (Log2) – – – 0.1 0.07 -0.27 -0.38 -0.32 -0.45 -0.45 -0.65 -0.51 0.38 0.43 0.44 0.48 0.46 0.36 0.32 0.68 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4527 -1.577 -1.8823 -1.5682 -1.6908 -1.6648 0.8611 -0.9206 -0.4675 -0.2075 -0.6316 -0.8363 0.1317 -1.5644 -1.2196 -1.2234 0.7673 -1.0873
Actin 0.0356 0.0003 0.001 0.0027 0.0451 0.0014 0.0989 0.0008 0.0141 0.0139 0.0041 0.0019 0.0879 0 0.0009 0.0624 0.0844 0.0268
Bud 0.0008 0.0003 0.0004 0.0001 0.0002 0.0001 0.007 0.0041 0.0025 0.003 0.0013 0.0022 0.0108 0 0.0008 0.001 0.0073 0.0003
Bud Neck 0.0069 0.0001 0.0002 0.0001 0.0002 0.01 0.018 0.0003 0.0012 0.001 0.0003 0.0008 0.0065 0 0.005 0.0219 0.0009 0.0004
Bud Periphery 0.0008 0.0002 0.0018 0.0001 0.0003 0.0001 0.0138 0.0065 0.0034 0.0116 0.0016 0.0065 0.0495 0 0.0011 0.0011 0.02 0.0004
Bud Site 0.0045 0.0082 0.0005 0.0001 0.0003 0.0005 0.025 0.0128 0.0132 0.0093 0.0065 0.0004 0.0227 0.0002 0.0128 0.0291 0.0051 0.0003
Cell Periphery 0.0004 0.0002 0.0001 0 0.0001 0 0.0019 0.0004 0.0002 0.0003 0.0001 0.0002 0.0008 0.0001 0.0007 0.0007 0.0006 0
Cytoplasm 0.0111 0.0114 0.0153 0.0068 0.0265 0.0179 0.0303 0.0352 0.031 0.0236 0.0522 0.0577 0.0735 0.0229 0.0214 0.0222 0.005 0.1019
Cytoplasmic Foci 0.0304 0.002 0.0217 0.0084 0.009 0.0097 0.0405 0.0091 0.0079 0.0273 0.0252 0.0121 0.0382 0.0024 0.0219 0.0239 0.0044 0.03
Eisosomes 0.0006 0.0003 0.0005 0.0003 0.0004 0.0001 0.0013 0.0001 0.0002 0.0002 0.0001 0.0001 0.0024 0.0002 0.0003 0.0012 0.0007 0.0001
Endoplasmic Reticulum 0.0018 0.0003 0.0006 0.0001 0.0004 0.0007 0.0132 0.0005 0.0012 0.001 0.0041 0.0008 0.0049 0.0002 0.0011 0.0015 0.0206 0.0006
Endosome 0.0142 0.0001 0.0025 0.0014 0.0061 0.0028 0.0385 0.0011 0.006 0.0323 0.0366 0.0069 0.078 0.0012 0.0116 0.0092 0.095 0.0041
Golgi 0.0087 0.0001 0.0003 0.0032 0.0074 0.0008 0.0143 0.0003 0.0028 0.01 0.0184 0.0083 0.0125 0 0.0028 0.0085 0.0243 0.0051
Lipid Particles 0.0322 0.0002 0.0014 0.0137 0.0113 0.002 0.0176 0.0005 0.0045 0.0292 0.0185 0.0036 0.0089 0.0002 0.007 0.0071 0.0103 0.0047
Mitochondria 0.0066 0.0002 0.0008 0.0037 0.0058 0.0008 0.0222 0.0006 0.0191 0.012 0.0183 0.0052 0.0197 0.0006 0.003 0.0011 0.058 0.0241
None 0.7715 0.9742 0.9463 0.916 0.8598 0.9456 0.5751 0.9222 0.8792 0.7522 0.783 0.8808 0.4858 0.9681 0.8761 0.7981 0.5035 0.7763
Nuclear Periphery 0.014 0.0001 0.0004 0.0004 0.0004 0.0037 0.0253 0.0003 0.0025 0.0032 0.0069 0.001 0.0134 0.0001 0.0018 0.001 0.1057 0.0005
Nucleolus 0.0045 0.0003 0.0004 0.0009 0.0001 0.0002 0.0079 0.0005 0.0006 0.0031 0.0005 0.0003 0.0014 0.0001 0.002 0.0004 0.0013 0.0003
Nucleus 0.0023 0.0006 0.0004 0.0012 0.0002 0.0005 0.0087 0.0011 0.002 0.0033 0.0023 0.0016 0.0051 0.0002 0.0046 0.0007 0.0114 0.0008
Peroxisomes 0.0386 0.0001 0.0031 0.0135 0.0241 0.0005 0.0238 0.0006 0.0038 0.0438 0.009 0.0039 0.0459 0.0001 0.016 0.0046 0.0031 0.0045
Punctate Nuclear 0.0108 0.0004 0.0014 0.0274 0.0007 0.002 0.0061 0.0018 0.0027 0.0069 0.0069 0.0038 0.0159 0.0002 0.0056 0.0033 0.0043 0.0184
Vacuole 0.002 0.0003 0.0008 0.0001 0.0011 0.0002 0.0068 0.0011 0.0013 0.0097 0.0023 0.001 0.0125 0.0024 0.0026 0.0008 0.0125 0.0002
Vacuole Periphery 0.0016 0 0.0001 0.0001 0.0004 0.0003 0.0037 0.0001 0.0005 0.0031 0.0016 0.0009 0.0037 0.0006 0.0012 0.0002 0.0216 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.67 0.8518 1.8962 2.8678 1.8531 6.6663 4.9522 6.6848 6.5236 3.4448
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant
Localization
Cell Percentages cytoplasm (26%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Agx1

Agx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Agx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available