Standard name
Human Ortholog
Description Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.05 0.13 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.16 0.17 0.2 0.11 0.06 0.1 0.09 0.12 0.15
Bud 0 0.05 0.11 0 0.09 0.07 0.1 0.1 0.09 0.06 0.11 0.06 0.05 0 0.1 0 0 0.05 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.51 0.48 0.53 0.56 0.46 0.5 0.5 0.47 0.41 0.38 0.45 0.54 0.53 0.54 0.45 0.34 0.33 0.09 0.25 0.12 0.2 0.17 0.08
Endoplasmic Reticulum 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.05 0.08 0.05 0 0 0 0 0 0
Endosome 0.24 0 0 0 0.18 0 0.1 0.06 0 0 0 0 0.18 0.21 0.12 0.25 0.28 0.31 0.37 0.21 0.21 0.26 0.23 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0.11
Mitochondria 0 0.41 0.33 0.48 0.12 0.46 0.32 0.38 0.61 0.55 0.6 0.62 0.14 0.08 0.22 0.06 0.05 0.05 0.16 0.23 0.35 0.2 0.21 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
SpindlePole 0.05 0 0 0 0.06 0.06 0.09 0.13 0 0.08 0.05 0.07 0.05 0.05 0 0.09 0.13 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.15 0.07 0.06 0.11 0.08 0.1 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 5 16 4 6 6 4 5 2 3 1 0 7 13 8 40 33 47 21 7 17 8 37 49
Bud 6 5 14 5 16 12 31 31 14 15 17 15 7 2 29 6 7 12 4 3 0 4 14 7
Bud Neck 0 0 3 0 1 0 3 2 1 1 1 0 0 0 0 0 4 0 2 2 3 2 7 7
Bud Site 0 0 0 0 3 3 8 7 1 7 1 4 0 1 0 0 0 0 – – – – – –
Cell Periphery 3 0 1 0 0 0 1 1 0 0 0 0 0 1 2 6 5 9 0 0 0 0 0 0
Cytoplasm 64 51 59 67 95 82 153 153 75 102 57 106 79 100 150 113 65 77 17 30 21 18 54 25
Endoplasmic Reticulum 10 5 7 2 3 5 5 5 1 8 6 1 11 13 8 12 16 11 4 1 2 0 5 10
Endosome 32 0 0 0 30 4 30 18 1 4 0 2 27 40 34 62 55 73 68 27 37 23 74 99
Golgi 5 0 0 0 0 0 0 0 0 0 0 0 1 3 1 11 5 7 14 2 3 1 9 34
Mitochondria 5 41 40 61 21 82 99 118 96 137 91 146 21 15 61 14 9 11 29 28 61 18 67 46
Nucleus 2 0 0 0 0 0 2 2 0 0 0 2 1 0 0 2 2 1 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 1 0 1 2 0 0 2 0 2 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 7 2 4 1 0 5 1 4 0 2 12 1 0 1 5 0 5 0 7 18
SpindlePole 7 0 4 2 10 10 27 40 3 20 7 17 8 10 8 22 25 25 4 3 5 3 10 7
Vac/Vac Membrane 11 6 3 4 6 3 9 4 0 7 2 10 4 5 6 25 30 16 11 13 13 9 25 13
Unique Cell Count 136 100 122 126 171 179 307 308 158 247 151 237 147 189 277 251 194 235 187 126 176 93 323 326
Labelled Cell Count 160 113 147 146 198 210 378 387 194 311 184 309 166 205 320 314 256 291 187 126 176 93 323 326


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.2 4.2 4.0 4.4 4.2 3.8 3.8 3.6 3.4 3.5 3.4 5.4 5.9 5.1 8.6 9.4 9.4 5.8 5.8 6.4
Std Deviation (1e-4) 0.8 0.6 0.5 0.8 0.6 1.6 0.8 1.2 1.2 0.8 0.9 1.1 0.9 0.8 0.8 1.5 1.9 1.7 1.2 1.0 1.2
Intensity Change (Log2) – – – -0.06 0.08 0.02 -0.12 -0.12 -0.21 -0.27 -0.27 -0.29 0.37 0.49 0.3 1.04 1.18 1.17 0.47 0.48 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.7 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8592 1.5782 1.419 1.1353 1.2902 1.3528 1.4906 2.399 1.9098 1.4178 1.7993 2.2131 3.2977 3.3393 2.9536 2.9028 2.524 2.9566
Actin 0.0492 0.0327 0.0318 0.0436 0.0237 0.0291 0.0824 0.0172 0.0521 0.0004 0.0202 0.0829 0.0644 0.0451 0.0667 0.0719 0.0099 0.0334
Bud 0.001 0.0033 0.0066 0.0018 0.0018 0.0008 0.0008 0.0028 0.0026 0.0021 0.0012 0.0018 0.0016 0.0054 0.0172 0.0023 0.0035 0.0005
Bud Neck 0.0122 0.0087 0.0059 0.0066 0.007 0.0396 0.0016 0.0015 0.0029 0.0004 0.0035 0.0156 0.0021 0.0088 0.0029 0.0012 0.0006 0.0095
Bud Periphery 0.0011 0.0026 0.0093 0.0027 0.0012 0.0007 0.0009 0.0021 0.0026 0.0053 0.0013 0.0023 0.0022 0.0043 0.025 0.0033 0.0041 0.0004
Bud Site 0.0136 0.0868 0.0147 0.0032 0.0109 0.0014 0.0172 0.0299 0.0137 0.0004 0.0038 0.0032 0.0091 0.0543 0.0289 0.001 0.0008 0.0008
Cell Periphery 0.0007 0.0015 0.0009 0.0004 0.0002 0.0003 0.0005 0.0007 0.0007 0.0002 0.0002 0.0003 0.0017 0.001 0.0015 0.0002 0.0005 0.0003
Cytoplasm 0.1467 0.2261 0.2662 0.2464 0.1827 0.2087 0.1735 0.3508 0.3064 0.2686 0.4195 0.251 0.1765 0.2729 0.229 0.1861 0.2147 0.3337
Cytoplasmic Foci 0.3002 0.2771 0.2078 0.2048 0.3047 0.3076 0.2418 0.2138 0.2456 0.2304 0.1983 0.2705 0.2229 0.2667 0.2025 0.1425 0.3337 0.2563
Eisosomes 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0001 0.0001 0.0002 0.0012 0.0002 0.0007 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0064 0.0039 0.0065 0.0053 0.0161 0.0051 0.003 0.0036 0.0041 0.0019 0.0044 0.0059 0.0269 0.0049 0.0059 0.0059 0.0073 0.0039
Endosome 0.2198 0.191 0.2505 0.2932 0.3108 0.1894 0.1944 0.1583 0.1793 0.3068 0.2184 0.1772 0.1342 0.1619 0.1364 0.2364 0.1708 0.1318
Golgi 0.0875 0.0623 0.0794 0.0288 0.0397 0.0783 0.0606 0.0347 0.0414 0.0266 0.023 0.05 0.06 0.045 0.0552 0.0368 0.0358 0.0516
Lipid Particles 0.0406 0.0164 0.009 0.0181 0.0076 0.0221 0.0277 0.0035 0.0069 0.0033 0.0187 0.0053 0.0235 0.0046 0.0076 0.0028 0.0093 0.0125
Mitochondria 0.011 0.0124 0.0266 0.011 0.005 0.0153 0.011 0.0026 0.0068 0.0087 0.0125 0.0129 0.0136 0.0061 0.0231 0.0064 0.0035 0.0054
None 0.0633 0.032 0.0357 0.0216 0.0155 0.0468 0.122 0.1483 0.0734 0.1255 0.0172 0.0367 0.1701 0.0682 0.153 0.2551 0.1011 0.117
Nuclear Periphery 0.0021 0.0008 0.002 0.0019 0.0015 0.0014 0.0054 0.0007 0.0012 0.0004 0.0011 0.0025 0.0099 0.0018 0.0018 0.0008 0.0024 0.0006
Nucleolus 0.0011 0.0003 0.0004 0.0004 0.0002 0.0002 0.0008 0.0002 0.0002 0.0001 0.0002 0.0001 0.0011 0.0004 0.0007 0.0001 0.0001 0.0001
Nucleus 0.001 0.001 0.0011 0.0018 0.001 0.0007 0.0011 0.0008 0.0009 0.0005 0.001 0.0008 0.0216 0.0025 0.0013 0.0005 0.0008 0.0005
Peroxisomes 0.029 0.0305 0.0332 0.0644 0.0421 0.0448 0.0434 0.0197 0.0397 0.0099 0.0418 0.0697 0.0411 0.0324 0.0314 0.0396 0.0933 0.0354
Punctate Nuclear 0.0059 0.0017 0.0024 0.0042 0.0056 0.002 0.0034 0.0012 0.003 0.0004 0.0018 0.0013 0.0044 0.0035 0.0018 0.0007 0.0023 0.0019
Vacuole 0.0053 0.0071 0.0073 0.0257 0.0177 0.004 0.0065 0.0066 0.0133 0.0056 0.0094 0.006 0.0093 0.0085 0.0056 0.0042 0.0041 0.0034
Vacuole Periphery 0.0018 0.0015 0.0024 0.0138 0.005 0.0016 0.0016 0.0009 0.0029 0.0025 0.0023 0.0037 0.0027 0.0014 0.0016 0.002 0.0012 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.2121 13.4701 10.6769 14.3989 11.9884 13.6908 18.9591 17.7578 19.4658 15.1117
Translational Efficiency 0.7033 0.7332 0.8075 0.7693 0.7601 0.7318 0.5289 0.7738 0.5605 0.5582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2119 1669 640 1165 1292 1598 1745 80 3411 3267 2385 1245

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 749.56 821.37 1206.62 848.55 537.13 763.74 888.02 1026.86 669.10 793.18 973.51 860.01
Standard Deviation 78.34 101.82 141.33 122.30 108.32 112.69 110.32 127.53 137.39 111.08 184.92 130.20
Intensity Change Log 2 — 0.131988 0.686856 0.178956 — 0.507810 0.725321 0.934896 — 0.300918 0.703038 0.543542

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004146 0.003332 0.025365 0.001641 0.000353 0.003540 0.009271 0.005802 0.002709 0.003434 0.013589 0.001909
Bud Neck 0.025283 0.012240 0.007393 0.025334 0.024035 0.017249 0.015212 0.007097 0.024810 0.014690 0.013114 0.024163
Bud Site 0.027691 0.031956 0.083576 0.039501 0.016750 0.030842 0.055580 0.033855 0.023547 0.031411 0.063092 0.039138
Cell Periphery 0.000084 0.000082 0.000184 0.000222 0.000417 0.000155 0.000113 0.000088 0.000210 0.000118 0.000132 0.000213
Cytoplasm 0.145928* 0.109976* 0.078380 0.241229* 0.141911* 0.150257* 0.159114* 0.124405* 0.144407* 0.129679* 0.137449* 0.233722*
Cytoplasmic Foci 0.441499* 0.462159* 0.301601* 0.160883 0.350120* 0.412050* 0.324750* 0.322426* 0.406887* 0.437649* 0.318538* 0.171264
Eisosomes 0.000620 0.000208 0.000089 0.000126 0.000429 0.000374 0.000104 0.000095 0.000548 0.000289 0.000100 0.000124
Endoplasmic Reticulum 0.000516 0.000917 0.002486 0.001042 0.002584 0.000921 0.001255 0.001098 0.001299 0.000919 0.001585 0.001045
Endosome 0.070306 0.111375 0.107516 0.104131 0.142837 0.117660 0.118050 0.103934 0.097778 0.114449 0.115223 0.104119
Golgi 0.131467* 0.130428* 0.037837 0.006633 0.078860 0.099582 0.029077 0.010139 0.111541* 0.115340* 0.031428 0.006858
Lipid Particles 0.012279 0.010595 0.020363 0.012183 0.056682 0.015620 0.013677 0.009379 0.029098 0.013053 0.015471 0.012003
Mitochondria 0.036381 0.022356 0.001514 0.006230 0.023123 0.028209 0.006572 0.013336 0.031359 0.025219 0.005215 0.006686
Mitotic Spindle 0.005508 0.015210 0.168183* 0.048141 0.009362 0.014051 0.084380 0.040255 0.006968 0.014643 0.106868* 0.047634
None 0.000460 0.000186 0.000087 0.003232 0.041863 0.000346 0.000904 0.000071 0.016143 0.000264 0.000685 0.003029
Nuclear Periphery 0.000050 0.000132 0.000414 0.000950 0.000979 0.000135 0.000494 0.000257 0.000402 0.000133 0.000472 0.000906
Nuclear Periphery Foci 0.000123 0.000379 0.010091 0.003941 0.006356 0.000386 0.002995 0.002609 0.002484 0.000383 0.004899 0.003855
Nucleolus 0.000290 0.000648 0.000118 0.005443 0.013101 0.000629 0.001761 0.000185 0.005143 0.000639 0.001320 0.005105
Nucleus 0.005019 0.003670 0.002952 0.049682 0.035959 0.004550 0.009370 0.003057 0.016738 0.004100 0.007648 0.046686
Peroxisomes 0.063492 0.042900 0.038938 0.028792 0.025564 0.053482 0.081471 0.112991* 0.049126 0.048076 0.070058 0.034203
Vacuole 0.025976 0.035761 0.110866 0.256191* 0.023771 0.043712 0.083856 0.208280* 0.025141 0.039650 0.091104 0.253112*
Vacuole Periphery 0.002883 0.005489 0.002048 0.004472 0.004945 0.006249 0.001996 0.000641 0.003664 0.005860 0.002010 0.004226

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.55 -6.33 5.03 3.62 7.25 -6.15 -8.65 -2.80 0.07 3.54 -1.83 -8.96 2.56 3.91 9.73
Bud Neck 8.46 11.77 1.34 -4.80 -7.07 3.15 4.09 8.35 7.31 6.13 8.01 8.68 1.74 -3.40 -4.04
Bud Site -1.80 -9.43 -1.79 -0.46 7.45 -5.95 -11.36 -2.72 0.16 4.17 -4.63 -13.34 -3.26 -0.64 6.91
Cell Periphery 0.15 -8.68 -2.03 -2.03 0.27 6.43 8.66 9.37 3.31 2.70 5.21 5.39 1.05 -1.15 -0.88
Cytoplasm 5.95 6.35 -11.28 -15.24 -14.77 -1.14 -3.12 -0.58 -0.21 0.48 3.04 -0.68 -11.69 -13.46 -10.52
Cytoplasmic Foci -2.94 17.96 44.02 43.48 12.72 -7.16 3.96 5.04 7.65 3.73 -5.62 17.50 40.87 45.77 23.00
Eisosomes 7.63 10.75 10.24 4.27 -2.84 0.69 5.03 5.36 7.15 2.05 5.70 11.39 11.07 7.73 -1.37
Endoplasmic Reticulum -2.02 -8.59 -5.05 -0.42 6.81 3.28 2.64 3.23 0.09 1.37 1.63 -1.94 1.51 -0.50 4.81
Endosome -10.16 -5.17 -2.64 5.09 2.00 3.76 4.52 5.51 3.99 3.62 -5.07 -2.94 3.17 6.73 5.17
Golgi 0.27 23.69 36.68* 29.87* 10.08 -4.36 13.44 17.79 22.72 8.44 -1.09 29.48 40.65 38.77 16.03
Lipid Particles 1.39 -4.95 1.34 0.08 5.35 10.22 11.18 12.52 4.26 4.07 9.09 7.89 10.19 2.04 3.97
Mitochondria 5.91 18.77 16.40 9.77 -2.92 -2.19 9.45 2.68 3.50 0.00 3.40 19.08 17.45 13.37 0.26
Mitotic Spindle -6.03 -15.63 -9.36 -6.86 10.58 -2.01 -16.75 -1.83 -1.49 3.24 -5.58 -23.30 -9.23 -7.22 9.96
None 2.19 2.81 -2.79 -3.13 -3.22 10.40 10.22 10.45 1.89 2.88 10.24 9.85 7.54 -3.03 -2.40
Nuclear Periphery -2.64 -6.64 -10.30 -9.11 -3.30 3.93 1.92 2.56 -3.87 1.38 3.19 -1.70 -5.17 -9.42 -4.22
Nuclear Periphery Foci -2.50 -7.98 -4.60 -4.31 4.51 8.63 3.76 0.73 -2.85 -1.12 7.77 -4.93 -1.98 -4.65 1.29
Nucleolus -1.44 3.34 -5.58 -5.02 -5.72 9.99 8.10 10.30 2.54 4.31 9.07 5.71 0.57 -5.09 -3.37
Nucleus 2.12 -0.51 -15.74 -16.22 -14.97 10.87 8.60 8.29 -1.83 1.23 10.57 5.71 -9.72 -16.08 -13.52
Peroxisomes 6.19 7.76 12.23 6.63 3.10 -8.84 -14.54 -2.61 -0.81 0.72 0.41 -5.58 8.23 7.92 12.10
Vacuole -4.36 -15.12 -33.95 -32.30 -14.34 -8.17 -19.04 -9.78 -9.20 -7.75 -8.64 -23.98 -35.56 -33.45 -23.49
Vacuole Periphery -5.02 3.95 -0.35 3.01 -1.97 -2.37 6.30 9.81 13.70 6.65 -5.99 7.47 1.15 4.45 -1.81
Standard name
Human Ortholog
Description Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mil1

Mil1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mil1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available