Standard name
Human Ortholog
Description GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.22 0.26 0.34 0.28 0.12 0.14 0.13 0.1 – 0.08 0.06 0.11 0.15 0.07 0.17 0.15 0.1 0.25 0.23 0.16 0.23 0.3 0.3
Bud 0.11 0.07 0.1 0.2 0.17 0.16 0.12 0.1 0.12 – 0.12 0.14 0.09 0.09 0.12 0 0 0 0.08 0.09 0.07 0.43 0.34 0.38
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0
Bud Site 0 0 0.05 0.08 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.35 0.36 0.36 0.22 0.26 0.25 0.22 0.27 0.19 – 0.13 0.15 0.33 0.36 0.48 0.43 0.56 0.58 0.08 0.1 0.05 0 0 0
Endoplasmic Reticulum 0.1 0.21 0.06 0.07 0.08 0 0.1 0 0 – 0 0.06 0.13 0.21 0.12 0.07 0 0 0.1 0.13 0.05 0.05 0.08 0.07
Endosome 0.07 0 0.06 0 0 0.06 0 0.05 0 – 0 0.07 0 0.07 0 0.08 0.07 0.1 0.08 0.09 0.1 0.06 0.05 0
Golgi 0.06 0.11 0 0 0 0 0 0 0 – 0 0 0 0.08 0.08 0 0.05 0 0.12 0.12 0.06 0.06 0 0
Mitochondria 0.22 0.14 0.06 0.18 0.16 0.4 0.43 0.29 0.67 – 0.72 0.52 0.39 0.13 0.23 0.05 0 0 0.14 0.08 0.3 0 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.09 0 0.07 0 0 0 – 0 0 0.09 0 0 0.06 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.09 0.08 0 0.06 0.1 0 – 0 0.05 0 0 0 0.14 0.07 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.08 0.13 0.06 0.12 0.12 0.1 0.2 0.07 – 0.13 0.14 0 0.05 0 0.09 0.1 0.08 0.07 0.11 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 114 68 112 117 117 67 89 57 50 – 33 30 12 21 9 61 36 23 134 97 104 13 51 65
Bud 44 23 41 69 70 90 72 44 65 – 51 71 9 13 16 12 5 10 45 37 42 26 58 82
Bud Neck 0 0 3 2 3 1 4 2 3 – 2 0 0 0 0 2 1 0 16 11 34 1 8 3
Bud Site 12 3 23 28 14 27 23 7 15 – 9 7 2 2 0 3 2 1 – – – – – –
Cell Periphery 11 9 10 8 7 6 11 4 7 – 5 15 2 4 1 12 22 12 1 0 3 0 0 0
Cytoplasm 141 113 155 76 110 139 138 124 101 – 54 80 35 51 65 154 136 131 42 44 34 1 3 8
Endoplasmic Reticulum 41 65 25 25 35 25 60 16 20 – 7 32 14 29 16 26 7 10 53 55 30 3 14 15
Endosome 30 10 24 14 18 31 28 22 14 – 2 34 4 10 6 30 16 23 44 36 63 3 8 7
Golgi 23 36 7 13 10 6 4 3 2 – 3 1 1 11 11 12 13 7 65 51 37 3 5 6
Mitochondria 88 43 25 62 65 223 271 130 351 – 295 270 41 18 31 17 4 10 76 35 194 2 11 14
Nucleus 1 2 4 7 7 3 10 7 6 – 14 8 0 1 1 0 5 1 2 0 1 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 2 0 2 0 0 0
Nucleolus 1 1 1 0 4 4 7 14 3 – 9 14 0 1 1 1 0 2 0 0 0 0 0 0
Peroxisomes 13 5 12 32 18 37 10 7 17 – 1 13 9 5 5 20 6 2 3 2 11 1 3 2
SpindlePole 10 3 48 30 33 18 36 43 15 – 15 25 1 2 0 50 16 21 5 2 12 1 3 4
Vac/Vac Membrane 27 24 57 21 48 66 63 91 37 – 54 75 2 7 2 32 24 18 38 45 53 1 2 4
Unique Cell Count 402 314 428 340 417 558 624 451 521 411 520 105 141 135 357 241 226 538 427 637 61 175 218
Labelled Cell Count 557 406 547 504 559 743 826 571 707 555 675 132 175 164 432 293 271 538 427 637 61 175 218


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 9.0 7.6 6.9 7.3 5.9 6.3 6.4 5.4 – 4.9 5.9 7.2 7.6 7.5 8.8 8.7 8.4 8.1 8.0 8.6
Std Deviation (1e-4) 1.2 1.5 1.0 1.2 1.4 1.2 1.2 1.2 1.2 – 0.9 1.2 1.2 1.4 1.8 1.5 1.4 1.8 2.4 1.5 1.4
Intensity Change (Log2) – – – -0.15 -0.06 -0.37 -0.28 -0.24 -0.49 – -0.65 -0.37 -0.09 0.0 -0.02 0.21 0.19 0.14 0.1 0.07 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.5 0.6 -5.7 -4.8 -5.1 -6.8 – 0 -8.8 -3.2 -2.7 -4.8 -3.1 -3.4 -4.8
Bud 4.2 3.1 3.0 1.0 0.1 1.4 – 0 1.9 -0.3 -0.1 0.8 -3.5 -3.7 -2.3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 1.6 -1.4 -0.4 -1.3 -3.1 -2.0 – 0 -3.5 0 -2.0 -2.8 -3.5 -3.0 -3.2
Cell Periphery 0 -0.7 -1.6 -0.7 -1.7 -1.1 – 0 0.5 0 0 0 0.9 4.0 2.0
Cytoplasm -4.2 -3.1 -3.8 -5.0 -2.8 -5.8 – -7.7 -7.4 -0.6 0 2.5 2.0 5.1 5.3
Endoplasmic Reticulum 0.8 1.4 -1.0 2.2 -1.6 -1.4 – 0 0.2 2.6 5.2 2.3 0.8 -1.7 -0.8
Endosome -0.9 -0.9 0 -0.8 -0.5 -2.3 – 0 0.6 -0.7 0.6 -0.5 1.5 0.5 2.2
Golgi 1.9 0.8 -0.8 0 0 0 – 0 0 0 0 0 1.6 2.7 0
Mitochondria 5.4 4.6 12.2 13.3 8.9 19.3 – 0 15.3 9.3 2.7 5.8 -0.7 -2.5 -0.8
Nucleus 0 1.0 0 0.9 0.8 0 – 2.5 0.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.3 0 – 0 3.0 0 0 0 0 0 0
Peroxisomes 3.9 1.2 2.7 -1.3 -1.3 0.4 – 0 -0.3 0 0 0 2.0 -0.2 0
SpindlePole -1.1 -1.6 -5.0 -3.2 -0.8 -5.1 – 0 -3.7 -3.3 -3.6 -4.1 1.2 -1.9 -0.8
Vacuole -3.3 -0.8 -0.7 -1.6 2.7 -3.2 – -0.1 0.5 -3.3 -2.7 -3.9 -1.9 -1.3 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0448 3.6892 2.7921 1.9711 2.6237 3.036 5.2518 5.7961 5.4785 4.7677 4.4981 5.4081 3.7173 4.3964 3.9531 4.0287 3.4229 3.9512
Actin 0.1239 0.056 0.1008 0.0542 0.1347 0.1194 0.1522 0.1009 0.1532 0.0817 0.0704 0.1169 0.1287 0.0815 0.1209 0.1082 0.0774 0.1512
Bud 0.0071 0.0089 0.0349 0.0387 0.0218 0.0035 0.0101 0.0157 0.0559 0.0267 0.0608 0.0044 0.0157 0.0123 0.0608 0.022 0.0157 0.0043
Bud Neck 0.0152 0.003 0.0091 0.0291 0.0019 0.147 0.0265 0.0036 0.0053 0.0112 0.003 0.1869 0.0127 0.0048 0.0062 0.0028 0.0251 0.2247
Bud Periphery 0.0069 0.0046 0.0357 0.0765 0.0188 0.0038 0.0093 0.0095 0.0522 0.0344 0.0502 0.0044 0.0193 0.0053 0.0837 0.0163 0.0185 0.0056
Bud Site 0.0464 0.1686 0.0498 0.0078 0.0097 0.0054 0.0855 0.1769 0.0697 0.0315 0.0331 0.0075 0.0644 0.197 0.0315 0.006 0.0055 0.0043
Cell Periphery 0.0015 0.0008 0.001 0.0013 0.0009 0.0008 0.0023 0.001 0.0015 0.0013 0.001 0.0007 0.0036 0.0011 0.0013 0.0006 0.0011 0.0007
Cytoplasm 0.0575 0.1699 0.0974 0.0896 0.1354 0.0564 0.0615 0.1904 0.1885 0.2237 0.1793 0.1003 0.1194 0.2077 0.1972 0.2524 0.2065 0.0919
Cytoplasmic Foci 0.1232 0.1467 0.1083 0.1157 0.0879 0.1493 0.1234 0.1202 0.0893 0.0744 0.0881 0.1373 0.1017 0.1182 0.0952 0.0956 0.1511 0.1202
Eisosomes 0.0013 0.0001 0.0002 0.0001 0.0002 0.0005 0.0005 0.0001 0.0002 0.0004 0.0001 0.0002 0.0005 0.0002 0.0002 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0127 0.0065 0.006 0.0058 0.0099 0.0044 0.0155 0.0046 0.0067 0.0112 0.0057 0.0069 0.0195 0.0149 0.0091 0.0039 0.0275 0.0054
Endosome 0.2426 0.2051 0.2443 0.3566 0.322 0.1851 0.1941 0.1581 0.1565 0.2569 0.3104 0.1646 0.2147 0.1865 0.1971 0.3129 0.2006 0.1459
Golgi 0.1793 0.1497 0.2102 0.1214 0.1975 0.2441 0.1994 0.1715 0.1646 0.1305 0.1541 0.202 0.2028 0.128 0.1302 0.1425 0.1533 0.1803
Lipid Particles 0.0157 0.0029 0.0073 0.0065 0.0042 0.0054 0.0115 0.0015 0.0018 0.0068 0.0007 0.0046 0.0076 0.0012 0.0023 0.0002 0.0277 0.0093
Mitochondria 0.0685 0.0172 0.0559 0.0241 0.0248 0.0431 0.0537 0.0147 0.0306 0.063 0.0176 0.0288 0.0551 0.0091 0.0308 0.0162 0.046 0.0197
None 0.0258 0.0089 0.0012 0.0016 0.0044 0.0038 0.0022 0.0017 0.002 0.0026 0.0016 0.0005 0.0015 0.0103 0.008 0.0007 0.0045 0.002
Nuclear Periphery 0.0082 0.0015 0.0028 0.0031 0.0015 0.0004 0.0146 0.0007 0.0026 0.0051 0.0017 0.0007 0.0028 0.0011 0.001 0.0008 0.0021 0.0004
Nucleolus 0.0043 0.0004 0.0018 0.0002 0.0002 0.0001 0.0004 0.0003 0.0003 0.0004 0.0001 0.0002 0.0002 0.0002 0.0002 0 0.0001 0.0001
Nucleus 0.0054 0.0014 0.0066 0.0012 0.001 0.0004 0.0041 0.0009 0.0016 0.0019 0.0024 0.0005 0.0009 0.0008 0.0008 0.0006 0.0009 0.0004
Peroxisomes 0.0274 0.0212 0.0115 0.0288 0.0034 0.0195 0.0169 0.0134 0.0054 0.0046 0.004 0.0245 0.0122 0.0047 0.0062 0.003 0.022 0.026
Punctate Nuclear 0.0035 0.0009 0.0021 0.0012 0.0008 0.0006 0.0025 0.0009 0.0008 0.0011 0.0008 0.0009 0.0007 0.0006 0.0007 0.0002 0.0009 0.0006
Vacuole 0.0185 0.0208 0.0084 0.0248 0.0131 0.0043 0.0088 0.0101 0.0077 0.0205 0.0098 0.0045 0.0125 0.0113 0.0114 0.0098 0.0079 0.0044
Vacuole Periphery 0.0051 0.0047 0.0047 0.0117 0.0058 0.0028 0.0048 0.0032 0.0036 0.0104 0.0051 0.0027 0.0036 0.0031 0.0052 0.0053 0.0055 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.175 12.4159 14.6728 20.4039 26.0111 14.3954 17.6682 19.1425 21.213 23.6564
Translational Efficiency 0.7141 0.8168 0.6843 0.6165 0.4653 0.7167 0.567 0.4563 0.4884 0.6002

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rgd2

Rgd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available