Standard name
Human Ortholog
Description Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (CVT) pathway; binds PtdIns(3,5)P2, PI3P and PI4P; WD-40 repeat containing protein and PROPPIN family member; relocalizes from the vacuole to cytoplasm upon DNA replication stress; mammalian homologs include: WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood (SENDA)
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.89 0.83 0.81 0.75 0.8 0.68 0.67 0.72 0.68 0.63 0.64 0.89 0.91 0.91 0.81 0.74 0.82 0.37 0.42 0.36 0.41 0.32 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.12 0.1 0.14 0.08 0.11 0.1 0 0 0 0 0.06 0.07 0.07 0.06 0.07 0.06 0.31 0.26 0.28 0.29 0.29 0.28
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.11 0.11 0.28 0.31 0.34 0.37 0.43 0.51 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.12 0.05 0 0.07 0 0.05 0.05 0 0.05 0.05 0 0.08 0.05 0 0.16 0.32 0.2 0.24 0.25 0.2 0.23 0.33 0.39
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 0 1 0 1 1 1 1 1 3 0 0 0 1 2 1 1 3 2 0 0 2
Bud 5 2 4 5 3 2 4 9 12 9 6 9 2 0 2 0 0 0 3 4 1 0 1 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 0 1 5
Bud Site 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 0 2 6 2 10 5 12 11 0 0 1 2 2 0 0 0 0 0 0 0
Cytoplasm 216 446 221 177 232 258 281 191 234 217 194 206 383 421 537 333 174 165 100 215 106 83 119 71
Endoplasmic Reticulum 2 9 8 3 2 2 3 2 4 0 6 1 7 3 4 16 10 8 2 2 11 2 2 6
Endosome 11 20 31 21 43 27 45 28 13 11 5 12 27 30 40 23 17 12 82 134 83 60 105 106
Golgi 0 0 0 1 1 1 0 0 0 0 0 0 1 0 0 3 2 0 0 2 0 0 0 0
Mitochondria 6 1 1 23 34 91 130 95 119 136 155 143 0 0 0 5 1 1 0 6 3 1 2 4
Nucleus 1 1 1 1 0 1 2 5 2 4 1 0 0 0 2 1 0 2 2 2 0 1 3 2
Nuclear Periphery 0 0 1 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0 3 5 10 2 2 3
Nucleolus 1 1 0 0 1 3 1 1 1 0 1 2 0 0 0 1 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 2 4 5 7 4 4 2 3 3 0 1 4 2 1 1 5 4 2 0 2 9
Vac/Vac Membrane 18 58 14 7 23 5 22 13 10 16 15 10 36 21 20 65 76 39 64 126 59 46 122 150
Unique Cell Count 246 502 266 219 310 324 415 283 326 317 306 320 430 461 588 409 234 200 270 518 294 204 369 381
Labelled Cell Count 260 541 286 241 344 397 502 351 412 405 401 400 456 476 610 452 285 230 270 518 294 204 369 381


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 7.6 6.5 6.1 6.3 5.8 5.4 5.3 5.6 5.4 5.3 5.4 7.6 7.6 7.5 10.0 12.0 10.5 6.7 6.7 7.8
Std Deviation (1e-4) 0.9 1.0 1.0 1.1 1.2 1.3 1.2 1.2 1.4 1.4 1.2 1.4 1.1 1.2 1.3 2.5 2.7 2.3 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.09 -0.04 -0.16 -0.27 -0.28 -0.22 -0.26 -0.31 -0.28 0.22 0.23 0.21 0.62 0.88 0.68 0.05 0.05 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1.3 1.6 1.1 0 1.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.8 2.6 0 0 0 0 0 0
Cytoplasm -0.6 -2.4 -1.1 -4.4 -4.2 -3.2 -4.1 -5.3 -5.1 2.3 3.3 3.5 -0.6 -2.4 -0.2
Endoplasmic Reticulum 0 0 0 0 0 -1.5 0 -0.8 0 -1.2 0 0 0.6 0.8 0.6
Endosome -0.7 0.8 -1.3 -0.3 -0.7 -3.5 -3.8 -4.9 -3.7 -2.5 -2.4 -2.4 -2.8 -1.7 -2.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.1 5.3 9.2 10.0 10.2 10.9 12.1 13.5 12.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.1 1.1 -2.5 0 -0.4 -1.3 -0.1 -0.2 -1.3 1.5 -0.4 -1.3 4.2 7.9 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0591 4.4859 3.7576 3.6891 3.7766 4.077 5.9278 5.8108 6.0049 5.8244 5.1594 5.9804 7.0791 8.0315 7.1119 7.216 7.0149 7.5256
Actin 0.0529 0.0004 0.0122 0.0401 0.0275 0.0048 0.0267 0.0003 0.0274 0.0085 0.0389 0.002 0.0189 0.0002 0.0185 0.0256 0.0031 0.0064
Bud 0.0014 0.0014 0.0014 0.001 0.0029 0.0005 0.0005 0.0058 0.0097 0.0011 0.0013 0.0022 0.0009 0.0004 0.0008 0.0014 0.0003 0.0006
Bud Neck 0.0147 0.0005 0.0053 0.0028 0.0044 0.0081 0.0009 0.0002 0.0011 0.0018 0.0015 0.0022 0.0177 0.0003 0.0005 0.0004 0.0005 0.0032
Bud Periphery 0.0015 0.0007 0.0009 0.0017 0.0028 0.0006 0.0005 0.0024 0.006 0.0009 0.0012 0.0029 0.0007 0.0003 0.0005 0.0008 0.0007 0.0002
Bud Site 0.0095 0.0065 0.0116 0.0013 0.0107 0.0008 0.0022 0.0039 0.0137 0.0015 0.0056 0.0007 0.0055 0.0017 0.0012 0.0008 0.0002 0.0009
Cell Periphery 0.0005 0.0002 0.0004 0.0006 0.0002 0.0001 0.0001 0.0003 0.0004 0.0002 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.5341 0.8652 0.7498 0.5829 0.5642 0.7031 0.6998 0.8359 0.7303 0.6298 0.6314 0.7716 0.6004 0.7622 0.7168 0.6748 0.6877 0.7608
Cytoplasmic Foci 0.0801 0.0313 0.0423 0.095 0.0876 0.0611 0.0605 0.0161 0.0347 0.0956 0.0856 0.0378 0.0656 0.046 0.0509 0.0723 0.0414 0.0466
Eisosomes 0.0004 0.0001 0.0002 0.0003 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0088 0.0065 0.0071 0.0061 0.0042 0.0055 0.0069 0.0048 0.0045 0.0028 0.0037 0.0049 0.0103 0.0095 0.012 0.0039 0.0035 0.0056
Endosome 0.1203 0.0341 0.0647 0.0987 0.1359 0.1049 0.0669 0.0318 0.0638 0.0786 0.104 0.0811 0.0985 0.0669 0.0811 0.0878 0.1138 0.0792
Golgi 0.016 0.0011 0.0072 0.0077 0.0153 0.0049 0.008 0.0007 0.0119 0.0094 0.0286 0.0062 0.0095 0.0015 0.0033 0.0033 0.0055 0.002
Lipid Particles 0.0161 0.0003 0.0022 0.0077 0.0143 0.0031 0.0068 0.0003 0.003 0.0044 0.0099 0.002 0.0059 0.0006 0.0012 0.0004 0.0012 0.001
Mitochondria 0.0079 0.0004 0.0044 0.0228 0.0046 0.0063 0.0027 0.0005 0.0037 0.0016 0.01 0.012 0.0027 0.0003 0.0015 0.0009 0.0142 0.0008
None 0.0669 0.0241 0.056 0.048 0.0267 0.0447 0.0744 0.0699 0.0681 0.1079 0.0217 0.0485 0.1042 0.0678 0.063 0.0491 0.0721 0.0554
Nuclear Periphery 0.0082 0.0032 0.0044 0.0073 0.0213 0.0079 0.0113 0.0024 0.0026 0.0025 0.0034 0.0023 0.0111 0.0066 0.0067 0.0033 0.0105 0.0046
Nucleolus 0.0024 0.0003 0.0003 0.0002 0.0015 0.0002 0.0003 0.0004 0.0002 0.0003 0.0009 0.0002 0.0018 0.0002 0.0002 0.0002 0.0001 0.0002
Nucleus 0.0037 0.0054 0.006 0.0047 0.0247 0.0071 0.0081 0.0056 0.0026 0.0035 0.006 0.0041 0.0064 0.0064 0.0043 0.0128 0.003 0.0057
Peroxisomes 0.0141 0.0004 0.0066 0.0175 0.0034 0.0025 0.0078 0.0001 0.0064 0.0257 0.0149 0.0013 0.0057 0.0003 0.0029 0.004 0.0005 0.0008
Punctate Nuclear 0.0044 0.0024 0.002 0.003 0.0119 0.0039 0.0035 0.0029 0.0012 0.0075 0.021 0.0025 0.0133 0.0007 0.0017 0.0024 0.0006 0.0022
Vacuole 0.0317 0.0138 0.0132 0.0344 0.0289 0.0231 0.0095 0.0133 0.0072 0.0138 0.0078 0.0118 0.0171 0.025 0.0277 0.0478 0.0268 0.0195
Vacuole Periphery 0.0042 0.0017 0.0018 0.0163 0.0068 0.0066 0.0023 0.0022 0.0013 0.0025 0.0024 0.0036 0.0032 0.0028 0.005 0.0078 0.0143 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9486 13.1254 14.1225 19.7207 9.5184 13.9408 16.423 17.9142 18.3515 18.9687
Translational Efficiency 1.0607 0.8643 0.9167 0.73 1.4893 1.0214 0.7579 0.5994 0.7404 0.6959

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (CVT) pathway; binds PtdIns(3,5)P2, PI3P and PI4P; WD-40 repeat containing protein and PROPPIN family member; relocalizes from the vacuole to cytoplasm upon DNA replication stress; mammalian homologs include: WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood (SENDA)
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg18

Atg18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available