Standard name
Human Ortholog
Description Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.12 0.05 0.05 0.06 0.05 0.05 0.06 0.05 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 0.08 0 0 0.05 0 0.07 0 0.08 0.1 0.09 0.08 0.15 0.05 0 0 0 0 0 0
Cytoplasm 0.4 0.19 0.29 0.3 0.4 0.37 0.35 0.38 0.47 0.41 0.28 0.36 0.34 0.79 0.68 0.67 0.06 0.11 0 0.09 0.15 0.08
Endoplasmic Reticulum 0.5 0.75 0.53 0.51 0.36 0.41 0.47 0.41 0.2 0.26 0.72 0.65 0.65 0 0 0 0.92 0.7 0.86 0.79 0.73 0.82
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.12 0.12 0.2 0.18 0 0 0 0 0 0 0 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.1 0.09 0.19 0.2 0.18 0.08 0.09 0.13 0.16 0 0 0.06 0.09 0 0.19 0 0.06 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 12 22 12 11 15 10 15 17 16 10 15 6 9 2 8 0 4 3 3 4 1
Bud 6 0 3 1 2 3 7 13 9 14 0 0 0 1 0 0 0 2 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 1
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cell Periphery 8 6 18 17 8 5 10 11 20 15 20 38 52 11 12 8 0 1 1 2 2 1
Cytoplasm 84 35 55 65 81 99 75 111 140 145 68 137 185 114 55 109 0 30 5 28 48 22
Endoplasmic Reticulum 107 141 100 113 72 111 101 118 59 91 176 248 355 5 2 6 14 191 192 246 246 242
Endosome 0 4 3 1 5 7 1 1 6 3 0 1 0 2 6 8 0 3 1 3 1 1
Golgi 0 0 1 1 0 0 0 0 0 0 0 1 0 0 1 2 0 1 0 1 1 3
Mitochondria 7 0 3 1 6 8 25 36 59 65 0 0 0 2 3 6 0 16 6 7 8 9
Nucleus 0 1 0 2 0 2 0 1 2 5 0 1 3 0 0 2 0 0 1 0 1 0
Nuclear Periphery 0 2 1 0 0 2 10 6 2 5 0 1 1 0 2 1 0 0 2 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 1 0 1 0 0 1 0 1 1 0 0 0 1 0 1 0 0 0 0 0 0
Vac/Vac Membrane 15 18 17 42 41 49 17 27 38 57 1 3 32 13 3 31 0 17 3 12 15 10
Unique Cell Count 212 187 187 220 201 268 214 291 300 352 243 381 550 145 81 162 16 274 223 313 336 295
Labelled Cell Count 234 220 223 257 227 302 257 339 354 417 275 445 634 158 86 183 16 274 223 313 336 295


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 9.3 9.1 10.6 9.3 7.7 7.1 6.8 6.7 7.1 8.8 8.9 8.4 8.2 10.5 10.4 9.3 9.4 9.5
Std Deviation (1e-4) 1.2 1.8 1.5 9.0 10.4 1.4 1.3 1.1 1.3 1.4 1.4 1.5 1.4 1.5 1.7 2.3 1.3 1.5 1.5
Intensity Change (Log2) -0.04 0.19 0.0 -0.28 -0.39 -0.46 -0.46 -0.4 -0.07 -0.06 -0.15 -0.18 0.17 0.16 -0.01 0.01 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 -0.4 -0.4 -0.4 -0.8 -0.6 -0.3 -0.9 -1.1 -1.3 0 -0.1 0 -0.6
Bud 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.5 2.0 0.4 0 0.7 0.3 1.7 0.6 2.2 2.8 2.7 1.8 3.5 0.8
Cytoplasm 2.4 2.5 4.6 4.2 3.7 4.5 6.3 5.3 2.2 4.2 3.9 10.9 7.8 9.2
Endoplasmic Reticulum -4.4 -5.0 -7.8 -7.2 -5.8 -7.5 -12.2 -11.1 -0.7 -2.5 -2.7 -13.1 -11.0 -13.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.8 5.0 6.5 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2.1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 2.7 3.0 2.6 -0.6 -0.1 1.0 2.1 -4.6 -5.2 -1.8 -0.2 -1.7 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9762 9.4226 9.1536 8.5242 8.3338 8.7604 6.344 7.6014 7.6674 6.7421 7.7906 7.2511 3.4756 4.9033 4.7469 3.9385 4.4906 4.4988
Actin 0.0223 0.0009 0.0093 0.0008 0.0334 0.0041 0.047 0.0004 0.0132 0.0175 0.0124 0.0062 0.032 0.0013 0.0125 0.0184 0 0.0074
Bud 0.0002 0.0001 0.0002 0 0.001 0.0001 0.0009 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0001 0.0003 0 0
Bud Neck 0.0049 0.0002 0.0041 0.0003 0.0008 0.0011 0.0029 0.0001 0.0003 0.0011 0.0004 0.0008 0.0007 0.0003 0.0004 0.0029 0.0002 0.0078
Bud Periphery 0.0004 0.0002 0.0003 0.0002 0.0032 0.0002 0.0013 0.0002 0.0001 0.0008 0.0003 0 0.0005 0.0002 0.0002 0.0005 0 0.0001
Bud Site 0.0026 0.0014 0.0042 0 0.0019 0.0001 0.0061 0.0003 0.0007 0.0006 0.0003 0.0001 0.0007 0.0019 0.001 0.0009 0 0.0001
Cell Periphery 0.002 0.0042 0.0037 0.0011 0.0009 0.0008 0.0017 0.002 0.0017 0.006 0.0012 0.0007 0.0007 0.0041 0.0011 0.0019 0.0001 0.0004
Cytoplasm 0.057 0.1664 0.1038 0.0787 0.0673 0.0828 0.059 0.0951 0.1135 0.0363 0.0754 0.0876 0.0578 0.158 0.1188 0.047 0.0692 0.0906
Cytoplasmic Foci 0.0107 0.0029 0.0071 0.0023 0.007 0.0038 0.022 0.0021 0.0096 0.0153 0.0089 0.0025 0.0126 0.0062 0.0047 0.01 0.0003 0.002
Eisosomes 0.0004 0 0.0002 0.0001 0.0005 0.0001 0.0007 0 0.0001 0.0005 0.0004 0 0.0004 0 0.0001 0.0001 0 0.0002
Endoplasmic Reticulum 0.7625 0.7825 0.788 0.8435 0.7185 0.8493 0.6908 0.8476 0.8064 0.8006 0.7794 0.8664 0.7477 0.7658 0.8168 0.8502 0.9209 0.863
Endosome 0.0297 0.01 0.0142 0.0119 0.0406 0.0189 0.0352 0.0106 0.0153 0.0311 0.0167 0.005 0.0413 0.0091 0.0112 0.0158 0.0019 0.0086
Golgi 0.0103 0.0008 0.0063 0.0023 0.0279 0.003 0.0164 0.0049 0.0075 0.0129 0.007 0.0017 0.0103 0.0015 0.0033 0.0153 0.0001 0.001
Lipid Particles 0.029 0.0048 0.0098 0.0042 0.027 0.0057 0.0235 0.0076 0.0101 0.0134 0.016 0.0037 0.0137 0.0089 0.0058 0.0153 0.0007 0.0018
Mitochondria 0.0126 0.0002 0.015 0.0301 0.0124 0.0047 0.0072 0.0066 0.004 0.0119 0.0436 0.0004 0.0083 0.0031 0.0018 0.0025 0 0.0005
None 0.0202 0.0025 0.006 0.0017 0.0026 0.0117 0.0208 0.0061 0.0105 0.0336 0.0167 0.0093 0.026 0.019 0.0034 0.0007 0.0001 0.0038
Nuclear Periphery 0.0172 0.0137 0.0095 0.0132 0.0339 0.0068 0.0294 0.006 0.0036 0.0055 0.0053 0.0056 0.0283 0.0111 0.0092 0.0061 0.0029 0.0079
Nucleolus 0.0005 0 0.0024 0 0.0002 0.0001 0.0005 0 0 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0001 0 0
Nucleus 0.0019 0.0019 0.0014 0.0015 0.005 0.0015 0.007 0.0008 0.0003 0.0006 0.0007 0.005 0.0027 0.0021 0.0013 0.0007 0.0013 0.0012
Peroxisomes 0.0077 0.0001 0.0066 0 0.0049 0.0005 0.012 0 0.0004 0.0045 0.0078 0.0012 0.0023 0.0001 0.001 0.0072 0 0.0004
Punctate Nuclear 0.0019 0.0004 0.0033 0.0001 0.0014 0.0003 0.0083 0.0002 0.0002 0.0029 0.0014 0.0004 0.0069 0.0007 0.0042 0.0008 0 0.0003
Vacuole 0.0049 0.0061 0.004 0.0071 0.0064 0.0038 0.0051 0.0083 0.0023 0.0038 0.0045 0.0029 0.0047 0.0059 0.0024 0.0032 0.0019 0.0026
Vacuole Periphery 0.0011 0.0005 0.0007 0.0008 0.0031 0.0006 0.0024 0.0008 0.0002 0.0007 0.0014 0.0002 0.0019 0.0006 0.0004 0.0003 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.0816 25.548 21.4305 27.7969 27.3878 29.8052 24.4277 32.5951 31.4568 36.598
Translational Efficiency 1.1898 1.1985 1.206 1.3114 1.2456 1.6587 1.2978 0.8602 0.9433 1.1579

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1697 527 1243 1706 1364 1827 1847 1849 3061 2354 3090 3555

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 795.10 956.55 1085.19 1159.25 841.13 988.78 1074.49 1074.38 815.61 981.56 1078.79 1115.11
Standard Deviation 137.20 148.67 149.12 178.43 118.07 132.80 141.12 162.13 131.04 137.17 144.49 175.35
Intensity Change Log 2 0.266704 0.448739 0.543984 0.233321 0.353251 0.353104 0.249639 0.400442 0.449015

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000991 0.001881 0.002893 0.008957 0.000729 0.000717 0.004135 0.003051 0.000874 0.000977 0.003635 0.005885
Bud Neck 0.002940 0.001863 0.001967 0.008473 0.001377 0.002111 0.002384 0.012279 0.002243 0.002056 0.002216 0.010452
Bud Site 0.006851 0.008506 0.008258 0.031703 0.004347 0.003646 0.008723 0.023595 0.005735 0.004734 0.008536 0.027486
Cell Periphery 0.001450 0.001637 0.001226 0.005798 0.001114 0.002096 0.001713 0.000913 0.001300 0.001993 0.001517 0.003257
Cytoplasm 0.141508* 0.120175* 0.079192 0.128549* 0.121036* 0.164974* 0.093843 0.194430* 0.132385* 0.154945* 0.087949 0.162814*
Cytoplasmic Foci 0.058391 0.049169 0.008817 0.024295 0.037751 0.028441 0.011566 0.067702 0.049194 0.033081 0.010460 0.046871
Eisosomes 0.000608 0.000398 0.000215 0.000901 0.000575 0.001037 0.000661 0.000302 0.000594 0.000894 0.000482 0.000589
Endoplasmic Reticulum 0.601222* 0.702208* 0.810262* 0.563468* 0.731563* 0.682498* 0.791667* 0.318998* 0.659303* 0.686911* 0.799147* 0.436316*
Endosome 0.015337 0.016687 0.008452 0.019847 0.009517 0.007642 0.007357 0.035849 0.012743 0.009667 0.007798 0.028170
Golgi 0.028712 0.016422 0.002763 0.008115 0.020985 0.021239 0.004058 0.006030 0.025269 0.020160 0.003537 0.007031
Lipid Particles 0.058500 0.025659 0.010782 0.032365 0.029334 0.036965 0.015247 0.052688 0.045504 0.034434 0.013451 0.042935
Mitochondria 0.013551 0.003167 0.000409 0.002258 0.008757 0.008180 0.001734 0.004086 0.011415 0.007058 0.001201 0.003209
Mitotic Spindle 0.000370 0.000833 0.000657 0.007781 0.000117 0.000065 0.000845 0.007805 0.000258 0.000237 0.000770 0.007794
None 0.013461 0.001942 0.000486 0.000948 0.004823 0.009684 0.001398 0.001998 0.009612 0.007951 0.001031 0.001494
Nuclear Periphery 0.004548 0.002290 0.003645 0.001505 0.000903 0.001292 0.002893 0.009643 0.002924 0.001515 0.003195 0.005737
Nuclear Periphery Foci 0.010554 0.010384 0.028151 0.017742 0.004531 0.003344 0.012245 0.031916 0.007870 0.004920 0.018644 0.025114
Nucleolus 0.001325 0.000156 0.000043 0.000484 0.000312 0.000205 0.000063 0.001118 0.000874 0.000194 0.000055 0.000814
Nucleus 0.015958 0.007473 0.011589 0.014639 0.006715 0.009941 0.012821 0.046879 0.011840 0.009388 0.012326 0.031408
Peroxisomes 0.004927 0.002378 0.000483 0.001359 0.002695 0.004806 0.001222 0.003018 0.003932 0.004262 0.000925 0.002222
Vacuole 0.014888 0.022524 0.019170 0.116480 0.010079 0.009601 0.024566 0.174382 0.012745 0.012495 0.022395 0.146595
Vacuole Periphery 0.003907 0.004246 0.000542 0.004333 0.002739 0.001515 0.000860 0.003320 0.003387 0.002127 0.000732 0.003806

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.92 -3.06 -6.71 -5.75 -4.63 0.05 -5.53 -5.50 -5.64 1.62 -0.59 -6.14 -8.12 -7.97 -3.04
Bud Neck 1.81 1.58 -6.63 -7.95 -7.96 -1.46 -2.55 -10.46 -9.42 -9.26 0.44 -0.25 -12.02 -11.91 -12.11
Bud Site -0.95 -1.17 -10.32 -8.90 -9.08 0.68 -3.81 -9.46 -9.76 -6.54 1.09 -3.19 -13.76 -14.24 -11.15
Cell Periphery -0.29 0.91 -6.13 -4.30 -6.60 -3.28 -2.98 1.54 4.26 4.55 -2.50 -1.39 -5.34 -2.61 -4.68
Cytoplasm 2.34 9.76 0.24 -2.13 -9.11 -7.18 4.50 -11.44 -5.24 -16.25 -4.70 10.27 -8.34 -3.21 -17.92
Cytoplasmic Foci 1.82 16.97 10.75 5.04 -8.38 3.11 10.03 -8.69 -12.65 -20.40 6.45 19.42 0.77 -6.17 -21.09
Eisosomes 2.17 5.11 -1.49 -2.79 -4.22 -2.84 -0.57 2.49 6.11 3.36 -2.68 1.21 0.40 2.89 -0.74
Endoplasmic Reticulum -5.97 -17.05 6.79 10.97 23.32 4.44 -4.89 36.70* 34.45* 44.76* -3.09 -16.33 28.27 30.74 46.44*
Endosome -0.74 5.90 -2.65 -1.24 -8.05 2.38 2.77 -16.66 -18.05 -18.49 3.94 7.04 -13.62 -16.58 -19.52
Golgi 4.75 14.93 10.93 4.15 -4.22 -0.13 10.90 9.79 11.97 -2.60 3.28 18.29 14.84 11.70 -4.77
Lipid Particles 9.10 16.48 8.22 -2.09 -10.14 -2.73 6.20 -7.15 -4.53 -13.44 4.63 16.63 1.57 -3.34 -16.43
Mitochondria 9.22 12.83 10.65 1.85 -5.08 0.43 6.27 4.00 4.45 -3.44 4.47 13.36 10.27 5.71 -4.94
Mitotic Spindle -0.72 -1.18 -5.65 -4.86 -5.31 0.99 -2.19 -7.19 -7.24 -6.31 0.13 -2.28 -8.99 -8.93 -8.16
None 6.38 7.68 7.31 1.39 -1.06 -3.20 3.72 2.84 5.78 -0.85 1.21 8.33 7.69 6.37 -1.05
Nuclear Periphery 2.19 1.08 3.78 0.96 5.25 -0.92 -4.13 -8.79 -7.80 -6.07 2.56 -0.54 -4.05 -6.64 -4.03
Nuclear Periphery Foci 0.26 -8.00 -4.95 -4.32 4.15 1.96 -6.49 -12.89 -13.71 -8.21 4.03 -8.95 -12.67 -15.95 -4.10
Nucleolus 6.05 6.67 2.35 -1.33 -1.71 0.67 1.53 -3.36 -4.77 -5.47 5.29 6.37 0.07 -3.90 -4.69
Nucleus 5.25 2.21 -1.38 -7.16 -3.86 -3.67 -5.45 -17.85 -16.49 -14.03 2.71 -1.05 -14.64 -17.44 -13.78
Peroxisomes 5.27 11.89 9.48 3.07 -5.46 -3.92 5.20 -0.82 3.43 -6.00 -0.73 11.97 6.82 5.09 -7.13
Vacuole -2.72 -2.97 -23.87 -20.84 -22.06 0.35 -7.71 -32.27 -32.41 -27.72 0.18 -7.60 -39.61 -39.33 -34.97
Vacuole Periphery -0.36 7.39 -0.01 0.30 -4.55 4.63 5.42 -1.08 -5.19 -5.91 3.95 8.92 -0.28 -3.25 -6.78
Standard name
Human Ortholog
Description Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erj5

Erj5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erj5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available