Standard name
Human Ortholog
Description Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.96 0.92 0.98 0.9 0.83 0.84 0.78 0.81 0.62 0.8 0.91 0.95 0.93 0.97 0.97 0.93 0.9 0.92 0.86 0.93 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.06 0 0.15 0.05 0.23 0.4 0.33 0.45 0.42 0.61 0.46 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.09 0.09 0 0.06 0 0.07 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2 3
Bud 0 2 1 0 0 1 4 4 6 5 5 8 0 1 1 0 0 0 0 0 0 1 4 4
Bud Neck 0 0 1 0 0 0 1 3 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 3 0 1 1 2 1 4 3 0 0 0 3 5 3 0 0 0 0 0 0
Cytoplasm 166 278 152 98 299 219 317 304 207 223 139 257 300 495 514 520 380 204 164 275 145 310 349 360
Endoplasmic Reticulum 0 1 0 0 1 3 1 1 0 2 0 0 1 0 1 19 20 11 0 0 0 1 1 4
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 3 0 4 1 2 2 1 2 1 3 5 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 5 0
Mitochondria 16 18 7 16 16 56 151 120 119 116 137 149 5 8 12 15 3 7 0 6 9 2 6 7
Nucleus 0 0 0 1 0 0 0 4 2 0 3 1 4 1 2 1 4 3 0 1 0 2 3 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 3 1 0 2 3 0 0 0 0 0 0 0 0 1 2 6 3
Vac/Vac Membrane 0 2 0 1 2 3 3 2 1 1 2 2 45 46 52 16 25 9 12 4 5 4 5 10
Unique Cell Count 170 290 159 107 306 243 381 360 265 276 226 322 331 519 554 538 393 219 182 298 170 334 397 418
Labelled Cell Count 182 304 163 116 321 282 479 442 338 348 294 425 358 551 586 576 440 241 182 298 170 334 397 418


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.9 3.6 3.7 3.8 3.4 2.8 3.2 3.0 2.7 3.0 3.2 5.0 4.8 4.7 5.0 6.1 6.4 4.7 4.8 5.3
Std Deviation (1e-4) 0.5 0.6 0.5 0.9 0.9 1.3 0.6 1.0 0.8 0.8 1.1 4.6 1.0 1.6 1.5 1.0 1.1 1.4 1.3 1.4 1.7
Intensity Change (Log2) – – – 0.02 0.05 -0.09 -0.38 -0.17 -0.28 -0.41 -0.29 -0.2 0.46 0.41 0.39 0.47 0.74 0.81 0.39 0.41 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 3.3 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4156 3.9728 3.41 2.6903 3.0912 3.4152 1.0623 1.0484 0.6607 0.6006 0.864 0.6611 1.3021 1.7408 1.1795 1.6837 1.5025 1.433
Actin 0.0502 0.0153 0.0217 0.0003 0.0027 0.0048 0.1055 0.0141 0.0006 0.0012 0.0728 0.004 0.0503 0.0026 0.004 0.0016 0.0492 0.001
Bud 0.001 0.0021 0.0042 0 0.0003 0.0003 0.0018 0.0086 0.0005 0.0003 0.0005 0.0002 0.0011 0.0039 0.0014 0.0002 0.0054 0.0001
Bud Neck 0.0023 0.0004 0.0019 0.0001 0.004 0.0039 0.0033 0.0005 0.0003 0.0002 0.0005 0.0005 0.0016 0.0001 0.0004 0.0001 0.0004 0.0003
Bud Periphery 0.0013 0.0017 0.0115 0 0.0004 0.0007 0.0024 0.0094 0.0007 0.0002 0.0006 0.0001 0.0022 0.0041 0.0028 0.0003 0.0029 0.0001
Bud Site 0.0101 0.0113 0.0221 0.0001 0.0122 0.0003 0.0283 0.0168 0.014 0.0006 0.0201 0.0003 0.0041 0.0075 0.0025 0.0013 0.0017 0
Cell Periphery 0.001 0.0004 0.0004 0 0.0001 0.0002 0.0007 0.0005 0.0002 0.0001 0.0002 0 0.0003 0.0003 0.0001 0.0001 0.0005 0
Cytoplasm 0.2259 0.4441 0.3993 0.4293 0.3951 0.4618 0.2638 0.4326 0.3254 0.3339 0.1572 0.3976 0.2806 0.4593 0.4001 0.314 0.3506 0.4878
Cytoplasmic Foci 0.0194 0.0101 0.0111 0.0015 0.0353 0.0258 0.0496 0.0076 0.0175 0.0564 0.0299 0.0176 0.013 0.005 0.005 0.0143 0.008 0.0099
Eisosomes 0.0004 0.0002 0.0004 0 0.0001 0.0001 0.0014 0.0004 0.0002 0 0.0002 0.0001 0.0007 0.0001 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0045 0.0082 0.0036 0.0014 0.0029 0.0039 0.0038 0.0028 0.0016 0.0021 0.0025 0.0014 0.0038 0.0013 0.0027 0.0015 0.0013 0.001
Endosome 0.0221 0.0021 0.0047 0.0003 0.0073 0.0035 0.0257 0.0019 0.005 0.0039 0.0198 0.0027 0.0158 0.001 0.0055 0.0153 0.0013 0.0014
Golgi 0.0053 0.0008 0.0024 0 0.0015 0.0006 0.0176 0.001 0.002 0.0006 0.01 0.0055 0.0072 0.0007 0.0025 0.0043 0.0025 0.0006
Lipid Particles 0.0072 0.0009 0.004 0 0.0032 0.0005 0.0143 0.0014 0.0047 0.0021 0.009 0.0009 0.0169 0.0013 0.0032 0.0172 0.0021 0.0059
Mitochondria 0.0021 0.0005 0.0213 0.0001 0.0004 0.0013 0.0244 0.0006 0.0104 0.0003 0.0075 0.0049 0.0111 0.0017 0.0069 0.0077 0.0309 0.0015
None 0.592 0.4645 0.4637 0.5647 0.508 0.4537 0.4119 0.4956 0.5925 0.5883 0.6088 0.5567 0.5576 0.4906 0.5429 0.6081 0.5177 0.4739
Nuclear Periphery 0.0082 0.0038 0.0024 0.0004 0.001 0.0026 0.0015 0.001 0.0007 0.0008 0.0025 0.0006 0.0071 0.0008 0.0024 0.0012 0.0004 0.0006
Nucleolus 0.0042 0.0006 0.0004 0 0.0019 0.0002 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001 0.0011 0.0002 0.0001 0.0006 0.0084 0.0001
Nucleus 0.0106 0.0143 0.0122 0.0011 0.0034 0.0202 0.0066 0.0019 0.0041 0.0011 0.0009 0.0025 0.0043 0.004 0.0041 0.0018 0.0054 0.0036
Peroxisomes 0.0071 0.0036 0.0092 0 0.0079 0.0069 0.0299 0.0006 0.0109 0.0012 0.0541 0.0009 0.0145 0.0135 0.0111 0.0062 0.0051 0.0109
Punctate Nuclear 0.0102 0.014 0.0022 0.0002 0.0108 0.0062 0.005 0.0014 0.0051 0.0053 0.0015 0.0033 0.0033 0.001 0.0011 0.0029 0.0007 0.0013
Vacuole 0.0137 0.001 0.0011 0.0002 0.0013 0.0023 0.0013 0.001 0.0023 0.0008 0.0007 0.0002 0.0023 0.0006 0.0006 0.0009 0.0029 0.0002
Vacuole Periphery 0.0011 0.0002 0.0003 0 0.0001 0.0004 0.0007 0.0002 0.0012 0.0001 0.0005 0.0001 0.0012 0.0001 0.0004 0.0004 0.0025 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2976 9.1331 6.2931 11.1457 10.4079 7.2936 9.7292 13.3557 13.3622 11.0962
Translational Efficiency 1.5373 0.9482 1.4948 1.1577 0.8495 1.2095 1.2194 0.6581 0.7665 0.9935

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
724 972 1774 926 1845 1548 1487 85 2569 2520 3261 1011

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 622.99 726.92 836.65 835.27 713.13 750.80 830.31 919.03 687.73 741.59 833.76 842.31
Standard Deviation 77.31 116.48 101.27 117.24 79.01 91.02 96.75 105.53 88.39 102.26 99.29 118.60
Intensity Change Log 2 — 0.222588 0.425415 0.423034 — 0.074264 0.219485 0.365947 — 0.145323 0.319170 0.392846

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000042 0.000494 0.000407 0.001092 0.000068 0.000268 0.000121 0.000158 0.000061 0.000355 0.000277 0.001014
Bud Neck 0.002401 0.012746 0.004139 0.012571 0.006918 0.012083 0.004395 0.002562 0.005645 0.012339 0.004256 0.011729
Bud Site 0.000544 0.007742 0.008352 0.030071 0.002525 0.006793 0.008724 0.003691 0.001967 0.007159 0.008521 0.027853
Cell Periphery 0.000111 0.000164 0.000106 0.000420 0.000101 0.000124 0.000133 0.000143 0.000104 0.000139 0.000118 0.000397
Cytoplasm 0.358641* 0.474353* 0.317300* 0.459329* 0.509329* 0.479111* 0.521820* 0.506215* 0.466862* 0.477275* 0.410560* 0.463271*
Cytoplasmic Foci 0.049215 0.164825 0.004957 0.020209 0.129633 0.157449 0.012216 0.031878 0.106969 0.160294 0.008267 0.021190
Eisosomes 0.000038 0.000096 0.000021 0.000050 0.000078 0.000084 0.000028 0.000086 0.000066 0.000089 0.000024 0.000053
Endoplasmic Reticulum 0.000662 0.001575 0.002713 0.002982 0.000530 0.000941 0.001727 0.002019 0.000568 0.001186 0.002263 0.002901
Endosome 0.001132 0.013174 0.001287 0.009318 0.004569 0.010354 0.002147 0.004408 0.003601 0.011442 0.001679 0.008905
Golgi 0.000819 0.002556 0.000069 0.002059 0.000541 0.002647 0.000148 0.000101 0.000619 0.002612 0.000105 0.001895
Lipid Particles 0.002194 0.006246 0.000732 0.001731 0.004110 0.004931 0.000841 0.002583 0.003570 0.005438 0.000782 0.001803
Mitochondria 0.000773 0.003471 0.001219 0.002015 0.001922 0.005711 0.000871 0.000376 0.001598 0.004847 0.001061 0.001878
Mitotic Spindle 0.001289 0.004257 0.004334 0.032965 0.002176 0.007671 0.009266 0.019495 0.001926 0.006354 0.006583 0.031832
None 0.015728 0.007983 0.005639 0.007709 0.003429 0.003435 0.005107 0.005705 0.006895 0.005189 0.005397 0.007541
Nuclear Periphery 0.000405 0.000644 0.001757 0.001774 0.000535 0.000597 0.001432 0.001914 0.000499 0.000615 0.001609 0.001786
Nuclear Periphery Foci 0.000179 0.001476 0.000668 0.002006 0.000291 0.000525 0.000936 0.001452 0.000259 0.000892 0.000790 0.001960
Nucleolus 0.001764 0.002126 0.000373 0.001483 0.000727 0.001359 0.000333 0.000663 0.001019 0.001655 0.000355 0.001414
Nucleus 0.557645* 0.234456* 0.620413* 0.292271* 0.304579* 0.252470* 0.364821* 0.296946* 0.375898* 0.245522* 0.503864* 0.292664*
Peroxisomes 0.001593 0.004972 0.000630 0.001563 0.002139 0.006061 0.000157 0.000358 0.001985 0.005641 0.000414 0.001462
Vacuole 0.004360 0.055275 0.024719 0.112580 0.025496 0.046322 0.063863 0.119051 0.019540 0.049775 0.042568 0.113124
Vacuole Periphery 0.000466 0.001369 0.000166 0.005801 0.000305 0.001066 0.000913 0.000194 0.000350 0.001183 0.000507 0.005329

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.37 -2.88 -4.73 -0.96 -1.41 -3.26 -4.80 -6.04 -1.18 -4.37 -3.56 -3.26 -4.80 -2.18 -2.53
Bud Neck -5.95 -1.85 -4.24 1.84 -3.45 -4.26 2.80 1.50 4.49 -0.37 -6.73 1.74 -2.57 2.54 -3.57
Bud Site -6.65 -5.34 -6.35 -2.81 -2.71 -3.77 -3.21 -1.93 0.38 0.51 -6.17 -5.29 -5.74 -3.11 -2.88
Cell Periphery -4.16 0.25 -1.60 -0.79 -1.65 -2.45 -1.28 -0.93 0.76 0.14 -4.86 -1.07 -1.76 -1.15 -1.62
Cytoplasm -8.02 6.44 2.28 11.19 -4.25 3.72 7.04 6.65 5.51 4.45 -0.87 15.67 13.95 14.42 3.01
Cytoplasmic Foci -16.39 13.12 10.35 24.37 -6.91 -4.60 29.96 20.91 23.10 -1.34 -11.00 32.54 29.48 37.57 -4.94
Eisosomes -10.49 3.19 -8.60 4.96 -12.16 -1.19 10.35 -0.86 -0.08 -5.99 -5.30 11.42 -0.89 4.59 -13.00
Endoplasmic Reticulum -2.39 -7.65 -9.15 -11.39 -2.75 -3.28 -12.96 -6.44 -5.64 -3.04 -4.46 -15.97 -16.33 -14.38 -5.71
Endosome -9.74 -2.03 -3.81 6.12 -3.32 -5.65 4.98 2.49 7.23 -1.51 -9.72 5.01 -0.66 7.07 -3.18
Golgi -1.98 1.42 0.25 2.51 -2.09 -3.77 5.28 5.25 4.60 -0.47 -4.32 3.36 -0.01 3.91 -2.05
Lipid Particles -6.70 3.54 0.38 7.53 -4.06 -1.57 9.69 5.26 5.94 -3.52 -4.64 10.30 4.39 8.40 -4.39
Mitochondria -4.15 -1.13 -1.10 3.93 0.16 -3.02 2.11 2.39 4.62 1.00 -3.91 1.41 1.12 5.11 -0.58
Mitotic Spindle -1.58 -3.01 -5.97 -5.01 -4.79 -2.77 -2.88 -1.27 -0.54 -0.56 -3.08 -3.90 -6.02 -4.60 -4.65
None 3.33 5.23 4.53 1.18 -1.99 0.14 -1.46 -2.28 -2.38 -1.65 2.21 3.33 0.94 -1.48 -2.77
Nuclear Periphery -4.39 -25.92 -17.82 -15.26 -4.14 -1.29 -17.30 -7.89 -7.63 -2.65 -3.05 -28.75 -18.27 -17.15 -5.43
Nuclear Periphery Foci -3.04 -9.50 -6.00 -1.58 -3.89 -3.41 -7.14 -5.56 -4.33 -1.70 -3.71 -11.29 -6.10 -3.76 -3.83
Nucleolus -1.30 5.17 2.77 3.84 -1.64 -3.74 5.57 2.75 5.23 -2.37 -3.99 7.29 1.16 3.73 -1.85
Nucleus 23.29 -5.84 13.88 -9.88 25.20 6.39 -11.23 -1.40 -2.93 1.64 17.84 -20.34 2.18 -10.69 17.36
Peroxisomes -3.88 1.34 1.21 8.26 -0.44 -6.41 8.14 7.71 10.45 -1.36 -7.67 5.50 4.45 12.05 -1.37
Vacuole -18.10 -16.75 -26.15 -18.82 -20.84 -9.18 -17.53 -7.97 -7.26 -5.44 -16.65 -20.28 -25.57 -21.36 -18.68
Vacuole Periphery -2.27 1.00 -1.67 -0.55 -2.18 -4.30 -1.22 3.20 5.15 1.91 -4.86 -0.07 -1.91 -0.69 -1.89
Standard name
Human Ortholog
Description Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
Localization
Cell Percentages cytoplasm (42%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ate1

Ate1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ate1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available