ORF | |
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Human Ortholog | |
Description | Aldehyde reductase; reduces aliphatic aldehyde substrates using NADH as cofactor; shown to reduce carbonyl compounds to chiral alcohols |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 7 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 8 | 2 | 7 | 3 | 4 | 1 | 1 | 0 | 7 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 171 | 127 | 54 | 149 | 229 | 241 | 259 | 185 | 173 | 220 | 165 | 192 | 181 | 177 | 190 | 180 | 118 | 158 | 159 | 118 | 77 | 116 | 219 | 184 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 1 | 2 | 6 | 1 | 1 | 3 | 3 | 3 | 0 | 1 | 0 | 10 | 1 | 5 | 1 | 0 | 0 | 0 | 2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 1 | 0 | 1 | 0 | 9 | 27 | 30 | 66 | 74 | 98 | 58 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 3 |
Nucleus | 0 | 2 | 0 | 2 | 0 | 8 | 9 | 6 | 12 | 8 | 7 | 16 | 0 | 1 | 0 | 6 | 3 | 3 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 1 | 0 | 1 | 19 | 27 | 17 | 28 | 40 | 26 | 44 | 29 | 46 | 0 | 3 | 0 | 10 | 5 | 7 | 0 | 0 | 1 | 2 | 1 | 0 |
Unique Cell Count | 173 | 128 | 55 | 163 | 251 | 264 | 317 | 244 | 248 | 323 | 268 | 285 | 181 | 182 | 190 | 198 | 124 | 165 | 169 | 129 | 91 | 125 | 241 | 206 |
Labelled Cell Count | 175 | 132 | 55 | 171 | 259 | 281 | 332 | 270 | 284 | 366 | 313 | 321 | 182 | 183 | 190 | 214 | 131 | 180 | 169 | 129 | 91 | 125 | 241 | 206 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 9.5 | 9.7 | 10.7 | 10.6 | 8.7 | 7.8 | 8.0 | 6.1 | 6.6 | 5.7 | 5.8 | 11.3 | 12.8 | 13.9 | 12.5 | 12.3 | 12.3 | 9.0 | 9.2 | 9.6 |
Std Deviation (1e-4) | 2.1 | 2.5 | 2.7 | 2.2 | 2.2 | 1.9 | 1.6 | 1.8 | 1.4 | 1.5 | 2.9 | 1.2 | 3.0 | 3.7 | 4.3 | 2.8 | 2.3 | 2.9 | 3.2 | 3.5 | 3.4 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.13 | -0.17 | -0.31 | -0.29 | -0.67 | -0.56 | -0.76 | -0.73 | 0.21 | 0.39 | 0.52 | 0.36 | 0.34 | 0.34 | -0.12 | -0.08 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -1.8 | -1.8 | -3.1 | -3.7 | -4.4 | -4.6 | -5.3 | -4.7 | 1.8 | -0.4 | 1.9 | -1.8 | -1.0 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.7 | 4.3 | 4.0 | 5.4 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.2 | 2.1 | 0 | 0 | 2.8 | 0 | 2.5 | 2.1 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3481 | 4.3654 | 3.8449 | 3.5212 | 2.6568 | 3.8299 | 1.8647 | 2.7502 | 2.7386 | 2.2161 | 3.1632 | 2.9186 | 2.2836 | 3.7682 | 3.6565 | 2.6303 | 3.7935 | 3.2242 |
Actin | 0.0075 | 0.0017 | 0.0073 | 0.0322 | 0.0152 | 0.0022 | 0.0069 | 0.0005 | 0.0086 | 0.0225 | 0.028 | 0.0088 | 0.0346 | 0.0026 | 0.0121 | 0.0981 | 0.0083 | 0.0022 |
Bud | 0.0035 | 0.0014 | 0.0019 | 0.0008 | 0.0003 | 0.0004 | 0.0004 | 0.0031 | 0.0009 | 0.0076 | 0.0043 | 0.0012 | 0.0006 | 0.0026 | 0.0055 | 0.0008 | 0.003 | 0.0005 |
Bud Neck | 0.0018 | 0.0003 | 0.0005 | 0.0018 | 0.0013 | 0.0009 | 0.0027 | 0.0004 | 0.0008 | 0.0031 | 0.0042 | 0.0013 | 0.0007 | 0.0014 | 0.0007 | 0.0006 | 0.0011 | 0.001 |
Bud Periphery | 0.0034 | 0.0005 | 0.0015 | 0.0005 | 0.0002 | 0.0006 | 0.0004 | 0.0012 | 0.0005 | 0.0129 | 0.0225 | 0.0015 | 0.0006 | 0.0014 | 0.0063 | 0.0008 | 0.0018 | 0.0005 |
Bud Site | 0.0086 | 0.0083 | 0.0016 | 0.0069 | 0.0024 | 0.0002 | 0.0006 | 0.0061 | 0.0019 | 0.0054 | 0.0057 | 0.0012 | 0.0054 | 0.0059 | 0.0048 | 0.0038 | 0.0424 | 0.0014 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.6511 | 0.8425 | 0.782 | 0.7922 | 0.4372 | 0.7874 | 0.7206 | 0.8217 | 0.7424 | 0.7345 | 0.5279 | 0.81 | 0.7997 | 0.9029 | 0.8275 | 0.7473 | 0.7401 | 0.8934 |
Cytoplasmic Foci | 0.0218 | 0.0081 | 0.0263 | 0.0484 | 0.0891 | 0.0125 | 0.0136 | 0.0051 | 0.0145 | 0.0156 | 0.0291 | 0.0114 | 0.0169 | 0.0036 | 0.0254 | 0.029 | 0.0659 | 0.0146 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0015 | 0.003 | 0.0025 | 0.0021 | 0.0023 | 0.0049 | 0.0013 | 0.0043 | 0.0068 | 0.0166 | 0.0031 | 0.0058 | 0.0083 | 0.0023 | 0.0019 | 0.0008 | 0.002 |
Endosome | 0.0328 | 0.0064 | 0.0117 | 0.0276 | 0.0853 | 0.0077 | 0.0076 | 0.0014 | 0.0097 | 0.0222 | 0.0742 | 0.0125 | 0.0145 | 0.0017 | 0.0081 | 0.0092 | 0.0208 | 0.0079 |
Golgi | 0.0161 | 0.0008 | 0.0035 | 0.0048 | 0.0643 | 0.0019 | 0.001 | 0.0001 | 0.003 | 0.0073 | 0.0127 | 0.0047 | 0.0073 | 0.0002 | 0.0048 | 0.0152 | 0.0429 | 0.0013 |
Lipid Particles | 0.0132 | 0.0001 | 0.0015 | 0.0017 | 0.034 | 0.002 | 0.0036 | 0 | 0.0027 | 0.0005 | 0.0217 | 0.0016 | 0.0065 | 0.0002 | 0.0035 | 0.0073 | 0.0256 | 0.004 |
Mitochondria | 0.0153 | 0.0004 | 0.009 | 0.0016 | 0.0037 | 0.0142 | 0.0006 | 0.0003 | 0.0061 | 0.0039 | 0.0112 | 0.0016 | 0.0032 | 0.0002 | 0.0014 | 0.0064 | 0.0065 | 0.005 |
None | 0.1874 | 0.1141 | 0.126 | 0.0373 | 0.2421 | 0.1503 | 0.2146 | 0.1506 | 0.1768 | 0.1345 | 0.1366 | 0.1158 | 0.0776 | 0.0584 | 0.0802 | 0.0421 | 0.0063 | 0.049 |
Nuclear Periphery | 0.0022 | 0.0009 | 0.0015 | 0.0022 | 0.001 | 0.0016 | 0.0058 | 0.0007 | 0.0051 | 0.0026 | 0.04 | 0.0082 | 0.0026 | 0.0042 | 0.0012 | 0.0012 | 0.0004 | 0.0033 |
Nucleolus | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0018 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Nucleus | 0.0065 | 0.0096 | 0.0066 | 0.0261 | 0.0027 | 0.0103 | 0.0096 | 0.0051 | 0.0089 | 0.0079 | 0.0104 | 0.0111 | 0.0161 | 0.0041 | 0.0031 | 0.0037 | 0.0019 | 0.0066 |
Peroxisomes | 0.0161 | 0.0012 | 0.0129 | 0.0056 | 0.0126 | 0.0025 | 0.0019 | 0.0001 | 0.0043 | 0.0007 | 0.0091 | 0.0004 | 0.0034 | 0.0001 | 0.0079 | 0.0301 | 0.0298 | 0.0022 |
Punctate Nuclear | 0.0012 | 0.0011 | 0.0017 | 0.0037 | 0.0042 | 0.0015 | 0.0032 | 0.0009 | 0.0059 | 0.0014 | 0.0327 | 0.0029 | 0.0016 | 0.0007 | 0.0038 | 0.001 | 0.0011 | 0.0021 |
Vacuole | 0.0037 | 0.0007 | 0.0011 | 0.0029 | 0.0014 | 0.0011 | 0.0015 | 0.0009 | 0.0019 | 0.0076 | 0.006 | 0.0016 | 0.0018 | 0.001 | 0.001 | 0.0006 | 0.0007 | 0.0014 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0008 | 0.0019 | 0.0044 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.6397 | 37.6839 | 44.0419 | 43.79 | 27.3278 | 52.6645 | 49.7709 | 48.5397 | 53.8628 | 46.224 |
Translational Efficiency | 0.9733 | 1.0766 | 0.9418 | 0.9848 | 1.0972 | 1.1327 | 0.9055 | 0.9505 | 0.876 | 0.9129 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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