Standard name
Human Ortholog
Description RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.07 0.13 0.07 0.09 0.06 0 0.05 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.06 0.08 0 0.22 0.16 0.15 0.08 0.19 0 0 0.19 0.06 0.12 0 0 0 0 0 0
Nucleus 0.11 0.14 0.13 0.05 0.06 0 0.08 0.05 0.08 0.09 0.07 0.06 0.05 0 0.05 0.08 0.07 0.06 0.07 0.13 0.08 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.88 0.88 0.92 0.88 0.92 0.86 0.86 0.83 0.86 0.87 0.85 0.92 0.86 0.8 0.9 0.91 0.9 0.84 0.74 0.78 0.81 0.68 0.58
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.24 0.29 0.33 0.26 0.35 0.29 0.31 0.31 0.45 0 0.07 0 0 0 0 0 0 0.1 0.09 0.15 0.19
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 8 4 4 6 3 2 0 0 0 0 0 0 1 3 1 0 1 4
Bud Neck 0 1 12 3 4 6 9 6 5 10 5 2 4 0 2 0 0 0 0 0 3 2 5 9
Bud Site 0 0 1 0 1 0 17 6 25 15 8 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 2 2 4 8 6 7 10 10 2 3 3 1 10 20 32 13 11 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 3 1 2 1 0 0 0 0 0
Endosome 1 2 5 12 28 46 35 32 22 6 18 33 2 6 12 1 1 4 1 5 3 1 5 9
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0 3 0
Mitochondria 23 10 5 0 5 22 41 15 82 62 50 30 38 4 9 93 26 35 0 2 1 0 3 2
Nucleus 18 38 52 12 25 12 40 18 31 36 22 22 11 10 13 40 27 18 12 38 32 5 6 6
Nuclear Periphery 0 0 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 4
Nucleolus 145 245 359 230 377 316 446 302 307 334 284 322 188 192 201 439 375 260 146 219 331 125 169 156
Peroxisomes 1 0 1 2 2 6 5 3 1 1 2 0 2 2 1 2 1 2 0 0 0 0 2 2
SpindlePole 1 11 42 60 126 112 134 125 107 120 102 171 4 15 11 1 4 3 6 12 44 14 36 50
Vac/Vac Membrane 1 6 1 0 13 1 5 3 3 3 1 2 0 3 5 3 8 5 1 10 4 2 7 11
Unique Cell Count 158 277 406 250 428 344 520 353 371 388 328 377 204 223 251 490 413 290 174 296 425 155 248 268
Labelled Cell Count 192 316 482 329 591 529 750 524 590 598 499 604 259 253 289 598 455 336 174 296 425 155 248 268


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.8 20.5 17.4 14.5 13.1 10.6 9.4 9.5 7.8 7.6 7.4 8.1 18.0 18.4 16.0 36.5 38.7 35.2 20.9 19.3 19.8
Std Deviation (1e-4) 6.1 6.4 4.4 3.7 2.9 2.3 2.1 2.2 2.0 2.0 1.9 1.9 5.7 5.7 5.2 13.9 16.3 15.6 4.7 5.2 5.3
Intensity Change (Log2) – – – -0.26 -0.42 -0.72 -0.89 -0.87 -1.15 -1.2 -1.23 -1.1 0.05 0.08 -0.12 1.07 1.15 1.01 0.27 0.15 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -1.5 -2.1 -1.1 -1.2 -1.1 -1.5 -0.3 -1.3 -2.6 -0.7 0 -1.9 -3.8 -3.5 -3.0
Bud Site 0 0 0 3.3 0 5.0 3.6 0 4.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.2 1.2 1.9 0 0 0 0 0 5.0 6.4 1.8 1.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2.8 3.9 6.6 4.1 5.0 3.6 0.4 3.3 4.9 0 0 2.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 4.6 2.6 9.2 7.5 7.2 4.6 7.9 0 2.0 8.5 3.8 6.1
Nucleus -3.4 -3.5 -4.6 -2.6 -3.7 -2.0 -1.6 -2.7 -3.3 -2.8 -3.4 -3.2 -2.3 -3.0 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 -0.2 1.6 -1.2 -1.2 -2.3 -1.0 -0.8 -1.3 1.4 -0.8 -2.9 0.6 1.1 0.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.7 6.9 7.5 5.9 8.3 6.5 7.2 7.0 11.0 -3.7 -1.5 -2.7 -7.1 -5.8 -4.9
Vacuole 0 3.1 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.1882 15.7235 12.905 14.7151 12.2415 13.0957 17.2055 21.1285 17.63 16.8607 15.2181 17.3646 16.0099 18.5141 16.6381 15.5279 15.8251 15.7277
Actin 0.0453 0 0.0076 0.0076 0 0.0107 0.0066 0 0.0062 0.0015 0.0013 0.0008 0.0003 0 0.001 0.0001 0 0.0093
Bud 0.0008 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0004 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0022 0.0001 0.0003 0.0024 0 0.0003 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002
Bud Periphery 0.001 0 0.0001 0.0002 0 0.0004 0.0006 0 0.0009 0.0001 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0036 0.0002 0.0012 0.0002 0 0.0003 0.0013 0 0.0016 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001
Cell Periphery 0.0004 0 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.0068 0 0.0011 0.0004 0.0001 0.0002 0.0004 0 0.0013 0 0 0 0.0004 0 0.0005 0.0006 0 0.0001
Cytoplasmic Foci 0.0118 0.0015 0.0129 0.0039 0.019 0.0114 0.009 0.0001 0.004 0.0008 0.0042 0.004 0.0065 0.0001 0.0005 0.0008 0.0012 0.0006
Eisosomes 0.0007 0 0.0002 0 0 0.0014 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0152 0 0.001 0.0004 0 0.0012 0.0016 0 0.0006 0.0002 0.0006 0.0001 0.0001 0 0.0004 0.0001 0 0.0001
Endosome 0.0463 0 0.0121 0.0142 0.0001 0.0035 0.0137 0 0.0084 0.0035 0.006 0.0019 0.0039 0 0.0017 0.0001 0 0.0009
Golgi 0.0136 0 0.004 0.0067 0.0001 0.0031 0.0062 0 0.0028 0.0033 0.0051 0.0027 0.0005 0 0.0002 0 0.0002 0.0007
Lipid Particles 0.0217 0.0002 0.0048 0.003 0.0135 0.0147 0.0187 0.0001 0.018 0.0204 0.0451 0.0073 0.0018 0.0001 0.0003 0.0003 0.0183 0.0011
Mitochondria 0.016 0.0001 0.0164 0.0012 0.0019 0.0049 0.0118 0.0002 0.0134 0.0211 0.0302 0.0023 0.0007 0 0.0001 0.0002 0.0004 0.0002
None 0.0102 0 0.0021 0.0002 0.0002 0.0004 0.0011 0 0.002 0.0001 0.0001 0 0.0004 0 0.0002 0.0004 0 0.0002
Nuclear Periphery 0.0509 0 0.0025 0.0007 0 0.0005 0.0123 0 0.0007 0.0018 0.0046 0.0017 0.0002 0 0.0025 0.0013 0.0003 0.0011
Nucleolus 0.6245 0.8584 0.8558 0.8695 0.9053 0.8851 0.8363 0.9008 0.8855 0.874 0.8405 0.9147 0.9099 0.9068 0.9107 0.8767 0.9257 0.9008
Nucleus 0.0968 0.1321 0.0667 0.0847 0.0571 0.0527 0.0497 0.098 0.0448 0.0693 0.0432 0.0597 0.069 0.0894 0.0781 0.1006 0.0506 0.0666
Peroxisomes 0.0053 0 0.0037 0.0025 0.0004 0.0032 0.003 0 0.0014 0.0013 0.0005 0.0002 0.0005 0 0.0001 0 0.0001 0.0002
Punctate Nuclear 0.0121 0.0073 0.005 0.0014 0.001 0.0053 0.0217 0.0004 0.0016 0.0007 0.0126 0.0034 0.0052 0.0031 0.0032 0.0182 0.0026 0.0178
Vacuole 0.0064 0.0001 0.0009 0.0004 0.0003 0.0002 0.002 0.0001 0.0037 0.0004 0.0011 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001
Vacuole Periphery 0.0083 0 0.0013 0.0003 0.0007 0.0002 0.0028 0 0.0019 0.0011 0.0039 0.0006 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 100.5707 100.6069 68.6188 62.6454 70.7855 56.4799 59.3027 45.2841 40.4895 71.8004
Translational Efficiency 1.231 1.2071 1.248 1.0933 1.2881 1.3035 1.1946 1.181 1.0775 1.0704

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
Localization
Cell Percentages nucleolus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Prp43

Prp43


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp43-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available