Standard name
Human Ortholog
Description Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.17 0.11 0.15 0.08 0 0 0 0 0.07 0.07 0.06 0.08 0.05 0.06 0 0.26 0.24 0.25 0 0.06 0.1 0.09 0.1 0.07
Bud 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.12 0.14 0.23 0.26 0.14 0.09 0.08 0.07 0.12 0.11 0.11 0.11 0.29 0.42 0.42 0.43 0.41 0.4 0 0.08 0.17 0.09 0.13 0.14
Cytoplasm 0.24 0.32 0.25 0.25 0.35 0.34 0.31 0.32 0.33 0.26 0.36 0.29 0.33 0.36 0.21 0.26 0.43 0.35 0.09 0.07 0 0 0 0
Endoplasmic Reticulum 0.61 0.6 0.45 0.37 0.13 0.2 0.25 0.28 0.24 0.27 0.15 0.27 0.47 0.39 0.57 0 0 0 0.69 0.67 0.63 0.51 0.49 0.56
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.24 0.18 0.42 0.26 0.15 0.23 0.32 0.29 0.23 0 0 0 0.07 0.07 0.07 0 0 0 0.15 0.09 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.13 0.42 0.16 0.24 0.28 0.22 0.21 0.19 0.23 0 0 0 0.1 0 0 0.06 0 0 0.05 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 53 62 106 41 10 15 33 31 58 56 49 70 4 8 4 11 22 14 15 41 89 22 28 18
Bud 10 19 20 10 17 39 31 24 38 55 45 49 0 0 0 0 3 4 3 4 2 3 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 20 14 4 9 7
Bud Site 0 0 1 0 0 5 0 1 0 2 6 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 38 82 168 132 83 86 77 62 95 96 95 92 25 60 48 18 37 22 11 49 153 20 36 34
Cytoplasm 78 182 182 127 209 318 278 270 271 225 312 246 28 52 24 11 39 19 36 47 11 10 12 8
Endoplasmic Reticulum 195 340 330 188 80 187 228 230 197 228 131 233 40 56 64 1 0 2 264 438 580 119 136 143
Endosome 0 1 12 12 20 23 29 20 14 9 26 14 1 1 0 0 0 0 2 5 4 1 3 3
Golgi 0 1 1 3 1 0 1 0 2 1 1 3 0 0 0 1 0 0 1 5 5 0 3 1
Mitochondria 9 12 118 123 107 391 238 129 190 276 249 199 1 0 0 3 6 4 10 13 27 34 24 18
Nucleus 1 0 1 0 2 4 6 3 8 6 15 5 0 0 0 1 0 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 3 8 11 8 7 9 5 8 0 0 0 0 0 0 0 1 2 0 0 0
Nucleolus 0 0 2 0 0 2 5 5 3 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 13 6 2 4 0 1 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 3 0 2 0 2 0 0 4 0 0 2 0 0 0 2 0 0 1 0 0 0
Vac/Vac Membrane 6 2 43 67 253 150 221 238 177 175 163 196 0 5 1 4 4 0 23 21 10 12 16 15
Unique Cell Count 321 568 727 513 596 942 909 836 817 850 858 861 85 144 113 42 90 55 385 654 916 236 279 256
Labelled Cell Count 390 701 998 713 787 1234 1158 1024 1061 1142 1104 1122 99 184 141 50 112 71 385 654 916 236 279 256


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.9 6.9 6.9 7.0 6.3 7.2 7.8 8.1 7.9 8.0 8.4 7.9 8.3 8.7 9.3 8.8 9.1 7.1 7.7 8.0
Std Deviation (1e-4) 1.0 1.2 1.7 1.4 1.9 1.5 1.5 1.4 1.7 1.7 1.8 1.8 1.2 1.3 1.3 1.8 2.3 1.9 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.01 0.01 -0.14 0.06 0.17 0.22 0.19 0.21 0.27 0.19 0.26 0.32 0.42 0.34 0.39 0.02 0.15 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.5 -8.3 -10.1 -7.9 -7.6 -4.8 -5.2 -5.9 -4.1 -2.5 -2.9 -3.2 2.0 2.4 2.2
Bud -0.9 0.1 1.5 0.8 0.1 2.0 3.5 2.5 2.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -4.2 -7.9 -8.2 -8.7 -6.0 -6.3 -6.4 -6.7 1.3 4.6 4.4 2.9 3.7 2.8
Cytoplasm -0.1 4.0 3.9 2.5 3.2 3.5 0.6 4.9 1.6 1.6 2.7 -0.9 0.2 3.7 1.6
Endoplasmic Reticulum -3.1 -12.5 -11.2 -8.6 -7.4 -8.8 -7.7 -13.2 -7.6 0.3 -1.4 2.2 -5.5 -8.3 -6.0
Endosome 0.9 2.0 1.1 2.0 1.0 0.1 -1.0 1.8 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.4 0.8 11.1 4.8 -0.4 3.4 7.4 6.0 3.4 -3.7 -5.2 -4.6 -1.6 -2.4 -1.8
Nucleus 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.9 -2.5 -2.8 -4.0 -3.5 -3.4 -2.8 -3.9 -3.9 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.4 15.9 6.3 10.1 11.6 8.8 8.4 7.7 9.4 0 -1.2 -2.2 0 -0.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1218 3.7517 3.5864 3.3277 2.9622 3.5652 5.2104 5.8388 5.5736 5.0694 4.9592 5.3288 4.1815 5.0612 4.6175 4.2984 3.6822 4.399
Actin 0.0538 0.0098 0.057 0.0388 0.0275 0.0361 0.028 0.0053 0.0281 0.0529 0.0402 0.0158 0.0714 0.0102 0.0631 0.015 0.0322 0.0185
Bud 0.001 0.0005 0.0018 0.0004 0.0004 0.0002 0.0008 0.0004 0.0033 0.0113 0.0009 0.0009 0.0005 0.0006 0.0062 0.0007 0.006 0.0002
Bud Neck 0.0114 0.0039 0.0073 0.0226 0.0054 0.0257 0.0081 0.0022 0.0065 0.0151 0.0169 0.0249 0.0036 0.0058 0.0065 0.0093 0.0023 0.0373
Bud Periphery 0.0031 0.0014 0.0074 0.0021 0.0013 0.0013 0.0037 0.0011 0.0184 0.0304 0.0077 0.0068 0.0022 0.0012 0.0209 0.0076 0.0068 0.0015
Bud Site 0.0061 0.0444 0.0257 0.0013 0.0025 0.0006 0.0093 0.0494 0.0218 0.0181 0.0025 0.0012 0.0104 0.0493 0.0185 0.002 0.0329 0.0005
Cell Periphery 0.0436 0.0556 0.0469 0.0355 0.0336 0.0282 0.04 0.0508 0.0659 0.0335 0.0871 0.0464 0.0439 0.0519 0.054 0.05 0.0072 0.0252
Cytoplasm 0.2701 0.4504 0.2719 0.2448 0.3714 0.3268 0.289 0.4226 0.3341 0.2476 0.489 0.4308 0.3141 0.5057 0.3301 0.1876 0.4259 0.4103
Cytoplasmic Foci 0.0188 0.007 0.0199 0.033 0.0086 0.0174 0.0162 0.0071 0.0143 0.0294 0.0137 0.0092 0.0283 0.0056 0.0126 0.024 0.0164 0.0055
Eisosomes 0.0036 0.0005 0.0013 0.0012 0.0017 0.0014 0.0019 0.0006 0.0019 0.0027 0.0023 0.0011 0.0014 0.0008 0.0022 0.0021 0.0001 0.0005
Endoplasmic Reticulum 0.4221 0.3731 0.4273 0.5175 0.4621 0.4997 0.4644 0.3961 0.4136 0.3547 0.2107 0.3813 0.37 0.3133 0.4108 0.6375 0.4023 0.4662
Endosome 0.0226 0.0072 0.0181 0.0162 0.0229 0.0085 0.0266 0.0069 0.0158 0.0464 0.0177 0.0121 0.018 0.0048 0.0093 0.0074 0.0108 0.0051
Golgi 0.0141 0.0045 0.0168 0.0189 0.0122 0.0082 0.0162 0.0048 0.0158 0.0331 0.0162 0.0094 0.0118 0.0026 0.0151 0.0073 0.0138 0.0062
Lipid Particles 0.0301 0.019 0.0244 0.0304 0.0251 0.0159 0.0226 0.0215 0.0191 0.0238 0.0191 0.0169 0.0309 0.0128 0.0213 0.0177 0.0121 0.0077
Mitochondria 0.0113 0.0003 0.0188 0.0009 0.0055 0.003 0.007 0.0003 0.0073 0.061 0.0338 0.0039 0.002 0.0005 0.0017 0.0008 0.0044 0.0005
None 0.0449 0.0107 0.0247 0.0242 0.0048 0.0145 0.0218 0.0185 0.0113 0.0049 0.0071 0.0237 0.0424 0.0223 0.0045 0.0026 0.003 0.0085
Nuclear Periphery 0.0164 0.0046 0.0059 0.0039 0.0043 0.0029 0.0098 0.0032 0.0044 0.0045 0.015 0.0031 0.0135 0.004 0.0051 0.0061 0.0023 0.0023
Nucleolus 0.0009 0.0002 0.0006 0.0001 0.0007 0.0002 0.0006 0.0002 0.0004 0.0029 0.0009 0.0002 0.0006 0.0003 0.0001 0.0002 0.0001 0
Nucleus 0.0023 0.0012 0.0017 0.0006 0.0009 0.0009 0.0071 0.0015 0.0014 0.0034 0.0025 0.0015 0.0058 0.0017 0.0011 0.001 0.001 0.0008
Peroxisomes 0.012 0.0001 0.0141 0.0019 0.0028 0.0022 0.0146 0.0001 0.0095 0.0116 0.0063 0.0041 0.0179 0.0002 0.0109 0.0066 0.0155 0.0001
Punctate Nuclear 0.0057 0.0006 0.0012 0.0006 0.0008 0.0025 0.0026 0.0005 0.001 0.0015 0.0058 0.0015 0.0032 0.0011 0.0008 0.0061 0.0007 0.0002
Vacuole 0.0048 0.0049 0.0059 0.0046 0.0044 0.0034 0.0084 0.0067 0.0055 0.0094 0.003 0.0048 0.0069 0.005 0.0049 0.0078 0.0036 0.0028
Vacuole Periphery 0.0014 0.0002 0.001 0.0004 0.0011 0.0005 0.0014 0.0002 0.0007 0.0019 0.0015 0.0005 0.0009 0.0002 0.0004 0.0006 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.0901 41.4226 44.8253 37.597 61.9556 59.8191 37.9559 32.3902 59.9444 48.8597
Translational Efficiency 0.4618 0.3344 0.3735 0.7434 0.5053 0.5185 0.434 0.6673 0.4009 0.4803

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
Localization
Cell Percentages ER (92%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Scs3

Scs3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scs3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available