Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0.05 – 0 0.05 0.05 0 0 0 0.11 0.13 0.08 0.13 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.24 0.26 0.1 0.36 – 0.33 0.44 0.35 0.49 0.46 0 0 0 0.05 0.05 0.17 0 0 0 0 0 0
Nucleus 0.92 0.9 0.87 0.83 0.86 0.71 – 0.67 0.63 0.63 0.58 0.64 0.9 0.84 0.78 0.81 0.76 0.78 0.86 0.84 0.8 0.78 0.73 0.75
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.12 0.2 0.22 0.15 0.24 – 0.28 0.33 0.32 0.31 0.3 0.2 0.16 0.19 0.1 0.06 0.11 0.1 0.06 0.08 0.13 0.17 0.14
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 – 2 7 10 9 4 0 0 0 1 0 0 0 3 0 0 2 8
Bud Neck 0 0 0 0 0 0 – 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 1 3 4 – 8 15 31 36 29 0 2 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 4 4 13 12 22 – 23 27 35 20 28 19 70 99 12 10 0 0 0 1 0 2 2
Endoplasmic Reticulum 0 1 0 0 1 1 – 1 0 0 0 0 0 1 1 3 2 0 0 0 0 0 0 2
Endosome 0 0 0 0 3 1 – 1 0 0 1 1 1 5 11 1 2 0 1 0 3 0 3 3
Golgi 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 1
Mitochondria 7 4 29 69 49 172 – 253 226 228 330 330 16 3 3 7 4 3 0 0 1 0 2 2
Nucleus 256 94 104 220 428 336 – 514 322 413 396 453 451 551 585 118 60 14 246 105 102 106 300 442
Nuclear Periphery 0 0 0 8 2 7 – 9 21 12 18 15 2 0 3 0 1 0 0 0 1 0 0 0
Nucleolus 42 13 24 58 76 116 – 217 167 209 209 215 99 103 142 14 5 2 28 7 10 17 70 83
Peroxisomes 0 1 0 0 0 0 – 0 0 0 0 0 0 1 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 0 – 1 4 5 2 3 0 2 7 0 1 0 0 0 1 2 4 7
Vac/Vac Membrane 1 3 0 1 9 5 – 5 6 4 10 5 3 3 8 7 6 2 4 3 2 6 19 33
Unique Cell Count 278 105 120 266 495 474 764 512 656 680 710 500 658 748 145 79 18 286 126 127 136 412 593
Labelled Cell Count 309 120 162 372 585 665 1034 795 948 1034 1086 591 741 870 164 92 21 286 126 127 136 412 593


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 10.1 6.2 5.9 6.6 5.3 – 5.2 4.9 5.1 5.0 5.0 8.4 9.3 9.2 10.5 10.5 11.8 7.0 8.2 8.9
Std Deviation (1e-4) 1.5 1.8 1.3 1.2 1.4 1.4 – 1.1 1.6 1.7 1.6 1.3 1.7 2.5 2.5 2.9 2.9 3.3 1.5 1.8 1.9
Intensity Change (Log2) – – – -0.08 0.08 -0.22 – -0.25 -0.36 -0.27 -0.32 -0.32 0.44 0.59 0.57 0.76 0.75 0.93 0.18 0.4 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 2.6 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0 0.6 – 0 0.9 0.9 0 0 0 2.5 3.1 1.7 2.5 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 -4.2 2.5 – 2.0 0 2.3 4.9 4.6 -7.9 0 0 -4.6 -3.5 0
Nucleus -1.0 -0.1 -3.5 – -4.3 -5.0 -5.1 -5.9 -4.9 1.1 -0.8 -2.1 -1.2 -1.9 -1.0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 -1.2 1.0 – 1.9 0 2.6 2.4 2.3 0 -1.2 -0.3 -2.4 -2.7 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0127 5.5648 4.2789 4.3786 4.1272 4.2886 3.5596 4.6418 4.2431 3.8389 3.1045 3.9609 5.8964 6.8661 6.6359 5.6853 5.619 5.955
Actin 0.0276 0.0001 0.0157 0.0435 0.051 0.0011 0.0193 0.0089 0.0062 0 0.0168 0.0004 0.0166 0 0.011 0.0254 0 0.0005
Bud 0.0007 0 0.0012 0.0012 0.0009 0.0004 0.0002 0 0.0003 0 0.0047 0.0001 0.0004 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0036 0.0001 0.0011 0.0024 0.0024 0.0022 0.0006 0.0003 0.002 0.0001 0.0018 0.0014 0.002 0.0001 0.0003 0.0005 0.0006 0.0022
Bud Periphery 0.001 0 0.0017 0.001 0.0018 0.0014 0.0003 0 0.0006 0 0.0078 0.0001 0.0006 0 0.0001 0.0002 0.0002 0.0001
Bud Site 0.0107 0.0002 0.0117 0.0116 0.0026 0.0007 0.0005 0.0006 0.0012 0 0.0147 0.0002 0.0025 0.0001 0.0007 0.0005 0.0002 0.0003
Cell Periphery 0.0003 0 0.0004 0.0002 0.0005 0.0004 0.0001 0 0.0005 0 0.0004 0 0.0002 0 0 0 0 0.0001
Cytoplasm 0.0076 0.0002 0.0033 0.0084 0.0013 0.0003 0.0006 0 0.0005 0 0.0055 0.0005 0.0039 0 0.0008 0.0006 0.0001 0.0001
Cytoplasmic Foci 0.0201 0.0008 0.0142 0.0204 0.0144 0.0003 0.0097 0.0001 0.0077 0 0.0293 0.0023 0.0046 0 0.0009 0.0033 0 0.0002
Eisosomes 0.0003 0 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0008 0 0.0002 0 0.0002 0 0.0001 0.0004 0 0
Endoplasmic Reticulum 0.0025 0 0.002 0.0074 0.0027 0.001 0.0004 0 0.001 0 0.003 0.0002 0.0027 0 0.0006 0.0007 0 0.0001
Endosome 0.0236 0.0011 0.0117 0.0624 0.0558 0.0029 0.0056 0 0.009 0 0.0409 0.0088 0.0121 0 0.0018 0.0022 0 0.0002
Golgi 0.0099 0.0011 0.0055 0.0078 0.0193 0.0007 0.0047 0.0001 0.0029 0 0.0194 0.0043 0.0026 0 0.0005 0.0024 0 0.0001
Lipid Particles 0.0152 0.001 0.0104 0.0066 0.0445 0.0027 0.0126 0 0.0098 0 0.0615 0.0062 0.0045 0 0.0009 0.0036 0 0.0005
Mitochondria 0.0106 0.0111 0.009 0.0111 0.0346 0.0123 0.0094 0.0001 0.0023 0.0001 0.0283 0.002 0.0016 0.0001 0.0014 0.0054 0.0005 0.0002
None 0.0043 0 0.0013 0.0034 0.0006 0.0002 0.0004 0 0.0004 0 0.012 0.0001 0.003 0 0.0014 0.0004 0 0.0001
Nuclear Periphery 0.0116 0.0012 0.0051 0.0282 0.0066 0.0174 0.0029 0.0004 0.0067 0.0003 0.0185 0.0013 0.0081 0.0002 0.0032 0.0144 0.0013 0.0008
Nucleolus 0.0353 0.0181 0.03 0.0086 0.1245 0.099 0.0297 0.0199 0.0238 0.0141 0.0974 0.0917 0.032 0.0233 0.0193 0.0367 0.1403 0.0823
Nucleus 0.7901 0.9635 0.863 0.6907 0.6089 0.8485 0.8942 0.9688 0.917 0.9631 0.5688 0.8747 0.8823 0.9754 0.9547 0.8855 0.854 0.9059
Peroxisomes 0.0071 0.0005 0.0058 0.0074 0.0078 0.0004 0.0048 0.0001 0.0038 0 0.011 0.0017 0.002 0 0.0003 0.0149 0 0.0001
Punctate Nuclear 0.0131 0.0003 0.0043 0.0608 0.009 0.0035 0.0027 0.0003 0.0024 0.0222 0.0474 0.0028 0.0148 0.0008 0.0016 0.002 0.0023 0.0058
Vacuole 0.0036 0.0004 0.0018 0.0102 0.0071 0.003 0.0007 0 0.0007 0 0.0066 0.0006 0.0023 0 0.0002 0.0003 0.0001 0.0002
Vacuole Periphery 0.0011 0.0002 0.0006 0.0065 0.0036 0.0018 0.0005 0 0.0005 0 0.0038 0.0005 0.0008 0 0.0002 0.0005 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.7345 25.6869 28.5912 27.8806 18.6278 43.0779 24.6734 26.294 28.823 27.0397
Translational Efficiency 1.491 1.0325 0.9278 1.4166 1.5507 1.1287 0.9635 0.8996 1.2057 1.0578

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
224 1181 742 1326 942 1537 1987 1760 1166 2718 2729 3086

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 986.32 1003.08 1193.88 1151.01 848.60 999.56 1068.02 1155.24 875.06 1001.09 1102.24 1153.42
Standard Deviation 133.45 172.08 206.83 181.64 134.36 149.54 199.18 169.30 144.74 159.73 208.93 174.72
Intensity Change Log 2 — 0.024309 0.275530 0.222773 — 0.236208 0.331782 0.445036 — 0.126186 0.301818 0.329832

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000878 0.000715 0.001188 0.001390 0.000473 0.000473 0.000960 0.001158 0.000551 0.000578 0.001022 0.001258
Bud Neck 0.002199 0.013832 0.024610 0.056414 0.003646 0.009938 0.031141 0.044752 0.003368 0.011630 0.029366 0.049763
Bud Site 0.000553 0.001858 0.001805 0.007095 0.000343 0.000628 0.002500 0.003421 0.000383 0.001162 0.002311 0.005000
Cell Periphery 0.000048 0.000057 0.000053 0.000237 0.000025 0.000025 0.000074 0.000076 0.000030 0.000039 0.000068 0.000145
Cytoplasm 0.000133 0.001208 0.000366 0.003353 0.000151 0.000368 0.001355 0.002783 0.000148 0.000733 0.001086 0.003028
Cytoplasmic Foci 0.000181 0.001467 0.000037 0.001064 0.000142 0.000160 0.000041 0.001055 0.000150 0.000728 0.000040 0.001059
Eisosomes 0.000046 0.000039 0.000047 0.000035 0.000032 0.000035 0.000037 0.000035 0.000035 0.000037 0.000040 0.000035
Endoplasmic Reticulum 0.002921 0.002163 0.002358 0.004150 0.001827 0.001772 0.001944 0.003674 0.002037 0.001942 0.002057 0.003879
Endosome 0.000122 0.000847 0.000091 0.003600 0.000151 0.000160 0.000115 0.001938 0.000145 0.000459 0.000108 0.002652
Golgi 0.000356 0.000580 0.000147 0.001383 0.000284 0.000125 0.000134 0.001943 0.000298 0.000323 0.000138 0.001702
Lipid Particles 0.000098 0.000129 0.000053 0.000194 0.000265 0.000154 0.000034 0.000242 0.000233 0.000143 0.000039 0.000221
Mitochondria 0.000741 0.002376 0.001905 0.007206 0.001246 0.000817 0.002008 0.004410 0.001149 0.001495 0.001980 0.005611
Mitotic Spindle 0.001279 0.006281 0.001353 0.042577 0.002429 0.003201 0.001376 0.031883 0.002208 0.004539 0.001370 0.036478
None 0.001917 0.001354 0.001664 0.007193 0.001427 0.000824 0.002141 0.002615 0.001521 0.001054 0.002012 0.004582
Nuclear Periphery 0.000039 0.000401 0.000240 0.003016 0.000075 0.000576 0.000325 0.001935 0.000068 0.000500 0.000302 0.002399
Nuclear Periphery Foci 0.000883 0.000698 0.000093 0.003812 0.000450 0.000493 0.000151 0.000884 0.000533 0.000582 0.000135 0.002142
Nucleolus 0.190226 0.114957 0.045305 0.040354 0.140775 0.127150 0.042293 0.029268 0.150275 0.121852 0.043112 0.034032
Nucleus 0.792281* 0.847482* 0.916934* 0.803863* 0.844424* 0.852122* 0.912293* 0.862525* 0.834407* 0.850106* 0.913555* 0.837319*
Peroxisomes 0.004566 0.001191 0.000952 0.000556 0.001399 0.000515 0.000449 0.000454 0.002007 0.000808 0.000586 0.000498
Vacuole 0.000384 0.001274 0.000618 0.008541 0.000218 0.000197 0.000435 0.002899 0.000250 0.000665 0.000485 0.005323
Vacuole Periphery 0.000148 0.001091 0.000180 0.003966 0.000215 0.000269 0.000194 0.002050 0.000202 0.000626 0.000190 0.002873

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.82 -6.36 -9.75 -14.75 -3.03 -0.60 -11.72 -15.22 -12.72 -5.26 -1.08 -13.90 -20.14 -18.69 -6.86
Bud Neck -10.64 -18.46 -24.89 -18.10 -10.41 -9.28 -26.61 -26.55 -22.03 -7.10 -13.32 -31.64 -36.03 -28.36 -11.59
Bud Site -3.26 -8.78 -7.08 -5.07 -4.50 -2.58 -5.82 -13.34 -11.54 -2.76 -4.22 -7.51 -12.04 -9.24 -4.85
Cell Periphery -0.72 -1.54 -3.87 -3.44 -3.49 0.00 -6.97 -10.31 -10.87 -1.22 -1.51 -7.27 -6.74 -5.73 -3.51
Cytoplasm -1.70 -3.76 -7.98 -3.00 -7.34 -1.26 -5.41 -6.40 -3.49 -3.38 -2.09 -5.79 -10.01 -4.54 -6.71
Cytoplasmic Foci -1.86 1.43 -1.20 1.59 -2.48 -0.33 1.66 -1.47 -1.03 -2.28 -1.92 2.07 -2.02 1.24 -3.31
Eisosomes 2.38 -2.78 0.36 -3.09 3.51 -1.30 -5.18 -5.37 -4.56 0.15 -1.06 -6.28 -5.33 -5.45 2.14
Endoplasmic Reticulum 2.70 1.25 -4.84 -9.34 -6.55 0.03 -1.96 -10.82 -8.99 -10.01 0.46 -1.28 -13.18 -12.74 -12.70
Endosome -2.44 0.07 -3.78 -1.65 -3.85 -0.28 0.35 -3.50 -3.45 -3.67 -2.36 0.37 -5.10 -3.22 -5.28
Golgi -1.29 1.56 -2.01 -0.81 -3.14 1.34 0.88 -1.30 -1.67 -1.55 -0.28 1.16 -1.90 -1.84 -2.38
Lipid Particles -0.49 0.82 -3.00 -1.82 -5.59 0.52 1.10 0.29 -0.68 -5.27 0.54 1.11 0.14 -1.43 -7.69
Mitochondria -2.52 -11.44 -8.83 -4.38 -6.08 0.68 -2.07 -5.10 -10.95 -6.19 -0.63 -2.74 -7.29 -8.93 -8.47
Mitotic Spindle -2.52 -1.90 -9.34 -7.73 -8.69 -0.53 -1.30 -7.88 -7.65 -7.47 -1.71 -1.69 -11.79 -10.86 -11.35
None 1.65 -0.06 -3.90 -4.64 -4.01 3.33 -4.31 -6.17 -9.21 -3.10 2.79 -3.97 -6.31 -7.63 -4.72
Nuclear Periphery -4.11 -11.45 -11.52 -9.86 -10.28 -1.53 -11.68 -13.53 -4.12 -10.95 -2.28 -14.60 -16.95 -8.83 -14.52
Nuclear Periphery Foci 0.50 2.00 -1.43 -2.01 -3.11 -0.15 1.91 -0.56 -0.32 -3.54 -0.19 2.70 -2.03 -1.94 -3.88
Nucleolus 7.10 12.30 13.41 12.74 1.48 2.27 15.71 19.99 20.81 6.03 5.65 19.42 23.08 24.02 5.19
Nucleus -4.68 -8.48 -0.41 5.90 11.34 -0.99 -6.48 -0.17 0.81 6.37 -2.59 -9.50 1.57 4.69 12.44
Peroxisomes 4.62 5.11 6.02 1.37 5.15 1.95 1.72 1.95 -0.00 2.00 2.87 3.10 3.61 1.34 4.18
Vacuole -2.04 -2.03 -5.86 -5.02 -5.48 -0.59 -3.38 -7.30 -7.16 -6.69 -2.68 -4.29 -9.00 -7.95 -8.29
Vacuole Periphery -3.18 -1.96 -3.39 -1.10 -3.26 -0.56 0.12 -2.63 -2.39 -2.71 -2.96 -0.16 -4.20 -2.23 -4.24
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Soh1

Soh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Soh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available