Standard name
Human Ortholog
Description RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.11 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.98 0.96 0.85 0.83 0.65 0.63 0.61 0.61 0.54 0.58 0.98 0.99 0.99 0.92 0.95 0.93 0.94 0.94 0.91 0.9 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.22 0.24 0.32 0.52 0.44 0.55 0.53 0.6 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 1 4 0 2 2 0 0 2 0 1 0 0 0 1 0 2 1
Bud 2 0 2 1 5 4 11 7 11 22 19 15 1 0 0 1 1 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 14 6 10 3 18 14 16 21 8 21 6 12 8 19 13 27 0 0 1 0 0 0
Cytoplasm 235 56 312 284 328 316 419 409 313 454 274 364 216 502 420 234 278 230 225 66 309 84 356 394
Endoplasmic Reticulum 0 0 3 0 3 2 19 4 6 17 7 19 4 4 0 14 8 7 8 0 10 0 2 4
Endosome 0 0 1 0 3 0 1 3 2 2 0 0 0 2 0 1 3 0 0 0 1 0 2 3
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 3 1 4 0 0 1 2 2 0
Mitochondria 9 3 6 65 91 121 334 286 285 394 304 353 1 2 1 5 3 2 1 1 6 1 3 2
Nucleus 0 0 0 0 4 2 5 8 3 5 1 0 3 2 0 2 3 0 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 1 1 2 0 0 1 6 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 1 1 0 1 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3
Vac/Vac Membrane 0 0 1 3 5 6 14 43 30 50 22 28 0 3 2 12 12 11 1 0 2 0 3 2
Unique Cell Count 236 57 320 295 385 381 648 651 514 740 503 632 221 509 423 255 293 246 239 71 338 94 382 421
Labelled Cell Count 247 59 340 360 450 457 824 777 671 965 640 808 231 528 433 291 323 282 239 71 338 94 382 421


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.1 6.7 6.3 6.1 5.6 5.3 5.5 5.3 5.2 4.9 5.1 7.8 7.9 7.4 7.9 8.4 8.6 6.7 8.0 8.4
Std Deviation (1e-4) 0.7 1.0 0.8 1.1 1.3 0.9 1.0 1.3 1.1 1.2 1.1 1.0 1.1 0.9 1.0 1.2 1.1 1.8 0.9 1.2 1.3
Intensity Change (Log2) – – – -0.09 -0.13 -0.27 -0.34 -0.29 -0.34 -0.37 -0.45 -0.4 0.21 0.24 0.14 0.24 0.32 0.35 0.0 0.25 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.4 2.8 1.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -1.3 -3.1 -1.3 -1.9 -1.0 -1.3 -2.4 -0.8 -1.0 -1.6 -2.0 1.6 0 3.0
Cytoplasm -0.9 -5.6 -6.3 -11.2 -11.6 -11.9 -12.1 -13.3 -12.8 0.2 1.2 2.0 -3.1 -1.7 -2.3
Endoplasmic Reticulum 0 0 0 2.0 0 0 1.5 0 2.0 0 0 0 3.2 1.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.8 8.4 10.2 15.2 13.4 15.8 15.8 16.9 16.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.4 4.1 4.5 3.4 3.5 0 0 0 3.5 3.2 3.4