Standard name | |
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Human Ortholog | |
Description | Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 8 | 20 | 20 | 18 | 21 | 20 | 25 | 50 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 |
Bud | 0 | 1 | 1 | 1 | 1 | 5 | 5 | 3 | 5 | 2 | 15 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 2 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 5 | 22 | 9 | 33 | 38 | 40 | 30 | 33 | 35 | 49 | 68 | 3 | 4 | 14 | 6 | 17 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 299 | 137 | 573 | 398 | 588 | 473 | 521 | 541 | 280 | 310 | 261 | 320 | 292 | 300 | 299 | 284 | 296 | 369 | 358 | 144 | 594 | 229 | 308 | 358 |
Endoplasmic Reticulum | 9 | 5 | 30 | 11 | 55 | 57 | 77 | 103 | 37 | 68 | 42 | 61 | 10 | 8 | 15 | 12 | 11 | 10 | 1 | 2 | 29 | 13 | 28 | 40 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Golgi | 2 | 0 | 0 | 0 | 15 | 1 | 6 | 3 | 2 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 3 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 4 | 7 | 41 | 90 | 75 | 111 | 125 | 168 | 193 | 3 | 0 | 2 | 1 | 3 | 0 | 1 | 0 | 3 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 3 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 23 | 1 | 14 | 1 | 18 | 4 | 15 | 25 | 12 | 4 | 16 | 8 | 0 | 1 | 6 | 8 | 12 | 5 | 2 | 5 | 13 | 1 | 1 | 2 |
Unique Cell Count | 331 | 142 | 613 | 411 | 676 | 568 | 688 | 740 | 421 | 492 | 457 | 565 | 300 | 310 | 317 | 294 | 313 | 381 | 375 | 161 | 662 | 250 | 348 | 406 |
Labelled Cell Count | 346 | 149 | 643 | 426 | 725 | 640 | 776 | 803 | 505 | 568 | 582 | 706 | 308 | 315 | 339 | 313 | 347 | 399 | 375 | 161 | 662 | 250 | 348 | 406 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.2 | 30.3 | 21.8 | 19.5 | 19.2 | 15.7 | 14.3 | 13.1 | 11.9 | 11.3 | 10.6 | 10.3 | 29.1 | 27.5 | 23.9 | 29.2 | 31.4 | 31.5 | 31.4 | 34.4 | 36.4 |
Std Deviation (1e-4) | 9.1 | 7.3 | 4.5 | 3.8 | 3.3 | 2.9 | 2.9 | 2.6 | 2.8 | 2.5 | 2.3 | 2.2 | 5.7 | 5.3 | 5.0 | 7.9 | 8.5 | 7.3 | 6.8 | 8.4 | 8.4 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.18 | -0.48 | -0.61 | -0.73 | -0.88 | -0.94 | -1.04 | -1.08 | 0.42 | 0.33 | 0.13 | 0.42 | 0.53 | 0.53 | 0.52 | 0.66 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 4.1 | 3.6 | 3.2 | 5.0 | 4.4 | 5.3 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.3 | 1.1 | 2.4 | 1.9 | 0.4 | 3.0 | 2.6 | 4.6 | 5.5 | -2.3 | -2.0 | 0.6 | -1.3 | 1.3 | -0.6 |
Cytoplasm | 2.4 | -3.9 | -5.5 | -8.7 | -9.8 | -11.2 | -12.6 | -14.2 | -14.7 | 2.5 | 2.1 | 0.5 | 1.9 | 0.7 | 2.3 |
Endoplasmic Reticulum | -1.8 | 2.3 | 3.4 | 4.1 | 5.6 | 2.5 | 5.2 | 2.8 | 3.8 | -1.1 | -1.7 | -0.1 | -0.5 | -1.0 | -1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.8 | 9.3 | 8.1 | 13.5 | 13.3 | 16.3 | 15.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.7 | 0.4 | -2.2 | -0.1 | 1.2 | 0.6 | -1.9 | 1.2 | -1.1 | 0 | -2.2 | -0.4 | 0.4 | 1.4 | -1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0443 | 0.001 | 0.0233 | 0.0133 | 0.0022 | 0.002 | – | – | – | – | – | – | 0.0047 | 0.0002 | 0.0104 | 0.0004 | 0.0151 | 0.001 |
Bud | 0.0015 | 0.0008 | 0.0029 | 0.0039 | 0.0012 | 0.0016 | – | – | – | – | – | – | 0.0012 | 0.0003 | 0.0021 | 0.001 | 0.0005 | 0.0007 |
Bud Neck | 0.0041 | 0.0011 | 0.005 | 0.0019 | 0.0013 | 0.0021 | – | – | – | – | – | – | 0.0006 | 0.0003 | 0.0006 | 0.0008 | 0.0017 | 0.001 |
Bud Periphery | 0.0023 | 0.0006 | 0.0022 | 0.0024 | 0.0014 | 0.0014 | – | – | – | – | – | – | 0.0019 | 0.0001 | 0.0031 | 0.0003 | 0.0003 | 0.0003 |
Bud Site | 0.0081 | 0.0012 | 0.0085 | 0.0029 | 0.0003 | 0.0003 | – | – | – | – | – | – | 0.0007 | 0.0002 | 0.0021 | 0.0002 | 0.0011 | 0.0001 |
Cell Periphery | 0.0045 | 0.0048 | 0.0072 | 0.0014 | 0.0004 | 0.0015 | – | – | – | – | – | – | 0.0012 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0002 |
Cytoplasm | 0.582 | 0.9088 | 0.7701 | 0.7249 | 0.6762 | 0.7443 | – | – | – | – | – | – | 0.8658 | 0.976 | 0.9097 | 0.8784 | 0.8539 | 0.8969 |
Cytoplasmic Foci | 0.0096 | 0.0023 | 0.0095 | 0.0066 | 0.007 | 0.0092 | – | – | – | – | – | – | 0.0112 | 0.0026 | 0.0065 | 0.0076 | 0.0326 | 0.0107 |
Eisosomes | 0.0006 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | – | – | – | – | – | – | 0.0001 | 0 | 0 | 0 | 0.0007 | 0 |
Endoplasmic Reticulum | 0.1051 | 0.0286 | 0.036 | 0.0144 | 0.0204 | 0.11 | – | – | – | – | – | – | 0.0312 | 0.0084 | 0.012 | 0.0258 | 0.0114 | 0.026 |
Endosome | 0.0338 | 0.0076 | 0.0206 | 0.0204 | 0.0441 | 0.0461 | – | – | – | – | – | – | 0.022 | 0.0043 | 0.0164 | 0.0294 | 0.0241 | 0.0341 |
Golgi | 0.0106 | 0.0013 | 0.0031 | 0.0036 | 0.0058 | 0.0058 | – | – | – | – | – | – | 0.0052 | 0.0007 | 0.0034 | 0.0012 | 0.0037 | 0.0099 |
Lipid Particles | 0.0063 | 0.0003 | 0.0045 | 0.0099 | 0.0009 | 0.0004 | – | – | – | – | – | – | 0.0017 | 0.0001 | 0.0004 | 0.0001 | 0.008 | 0.0001 |
Mitochondria | 0.0382 | 0.0013 | 0.005 | 0.0471 | 0.0125 | 0.0048 | – | – | – | – | – | – | 0.0142 | 0.0002 | 0.0016 | 0.0004 | 0.0055 | 0.0061 |
None | 0.0024 | 0.0002 | 0.0037 | 0.0004 | 0.0117 | 0.0006 | – | – | – | – | – | – | 0.0041 | 0.0001 | 0.0003 | 0.0002 | 0.0044 | 0.0004 |
Nuclear Periphery | 0.0267 | 0.004 | 0.0062 | 0.0065 | 0.0158 | 0.011 | – | – | – | – | – | – | 0.0039 | 0.0005 | 0.0025 | 0.0039 | 0.0024 | 0.002 |
Nucleolus | 0.0007 | 0 | 0.0024 | 0.0001 | 0.0018 | 0.0001 | – | – | – | – | – | – | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 |
Nucleus | 0.0058 | 0.002 | 0.004 | 0.0034 | 0.0129 | 0.0033 | – | – | – | – | – | – | 0.002 | 0.0004 | 0.0011 | 0.0013 | 0.0018 | 0.0009 |
Peroxisomes | 0.0092 | 0 | 0.0073 | 0.0288 | 0.0002 | 0.0001 | – | – | – | – | – | – | 0.0023 | 0 | 0.0012 | 0.0001 | 0.003 | 0.0001 |
Punctate Nuclear | 0.0029 | 0 | 0.0016 | 0.0003 | 0.0252 | 0.0002 | – | – | – | – | – | – | 0.001 | 0 | 0.0001 | 0.0001 | 0.0065 | 0 |
Vacuole | 0.0802 | 0.0302 | 0.0662 | 0.0908 | 0.1191 | 0.0429 | – | – | – | – | – | – | 0.0207 | 0.0049 | 0.0213 | 0.0391 | 0.0179 | 0.0061 |
Vacuole Periphery | 0.0211 | 0.0039 | 0.0102 | 0.0171 | 0.0396 | 0.0121 | – | – | – | – | – | – | 0.0043 | 0.0006 | 0.0049 | 0.0092 | 0.0047 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 166.4945 | 138.5035 | 160.6439 | 171.3563 | 138.7082 | 139.5854 | 143.9584 | 140.9797 | 145.2744 | 154.385 |
Translational Efficiency | 0.7347 | 0.7553 | 0.6737 | 0.6483 | 0.6319 | 0.7027 | 0.649 | 0.4988 | 0.589 | 0.5624 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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