Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.06 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.96 1.0 0.98 0.96 – 0.76 0.79 0.68 0.67 0.69 1.0 1.0 0.99 1.0 1.0 1.0 0.98 0.94 0.89 0.89 0.97 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.18 0.12 0.26 0.2 0.18 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 2 8 3 5 9 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 1 0 0 0 0 – 0 0 6 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 3 – 12 13 8 9 17 0 1 0 0 0 1 0 0 1 0 0 0
Cytoplasm 111 178 131 145 254 213 – 220 179 191 106 174 83 125 148 33 38 39 104 172 129 34 111 101
Endoplasmic Reticulum 0 1 3 0 2 4 – 51 28 72 32 46 0 0 1 2 0 3 0 1 4 0 0 1
Endosome 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 5 7 2 5 9 0 0 1 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 0 1 – 2 8 3 14 20 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 – 0 1 2 0 0 0 0 0 0 0 0 0 3 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 2 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 1 1 – 2 0 0 1 0 0 0 0 0 1 0 0 3 0 1 1 2
Unique Cell Count 111 181 137 145 258 222 288 228 279 159 254 83 125 150 33 38 39 106 183 146 39 114 109
Labelled Cell Count 111 181 139 146 258 224 296 244 287 174 277 83 126 150 35 39 43 106 183 146 39 114 109


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 173.7 159.5 94.3 154.4 141.1 106.9 – 84.4 76.9 71.1 66.6 64.7 166.8 155.9 152.9 162.6 189.4 175.2 – 181.7 185.0
Std Deviation (1e-4) 28.7 35.3 40.7 43.0 37.1 32.7 – 22.6 25.6 19.5 20.7 18.1 28.4 33.0 34.6 70.5 47.4 54.4 – 42.5 55.7
Intensity Change (Log2) – – – 0.71 0.58 0.18 – -0.16 -0.3 -0.41 -0.5 -0.54 0.82 0.72 0.7 0.79 1.01 0.89 – 0.95 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 2.4 0 0 2.7 0 0 0 0 0 0
Cytoplasm 2.5 1.7 0.1 – -4.9 -4.4 -6.2 -6.2 -6.2 1.9 2.4 1.6 1.2 1.3 1.3
Endoplasmic Reticulum 0 0 0 – 4.5 3.3 5.9 4.8 4.5 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 3.2 3.0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 198.343 286.702 239.3508 194.2714 156.8257 207.1219 149.902 271.6551 240.5475 198.7854 151.0678 206.1512 158.7258 296.4152 259.5644 206.5864 230.168 276.7203
Actin 0.0114 0.0005 0.0048 0.0067 0.0018 0.1258 0.0974 0.0007 0.0198 0.001 0.0237 0.0204 0.0373 0.0005 0.0155 0.0026 0.0072 0.0043
Bud 0.0073 0.0027 0.0059 0.0072 0.0064 0.0106 0.0073 0.0027 0.0068 0.0095 0.0086 0.004 0.0031 0.0021 0.0064 0.0021 0.0061 0.0033
Bud Neck 0.0013 0.0008 0.0009 0.0025 0.0006 0.0033 0.003 0.0007 0.0011 0.0016 0.0081 0.003 0.0018 0.0005 0.0021 0.001 0.0017 0.0035
Bud Periphery 0.0065 0.0016 0.0035 0.0084 0.0026 0.0102 0.0119 0.0018 0.004 0.0622 0.0122 0.0031 0.004 0.0011 0.0079 0.0011 0.0029 0.002
Bud Site 0.0027 0.0016 0.0022 0.0023 0.0014 0.0012 0.01 0.0014 0.0041 0.0009 0.0613 0.0008 0.012 0.0016 0.0108 0.0009 0.0009 0.0008
Cell Periphery 0.0623 0.0317 0.0609 0.0349 0.0374 0.0215 0.025 0.0154 0.0469 0.1056 0.0126 0.0178 0.0429 0.0264 0.0188 0.0205 0.0149 0.0129
Cytoplasm 0.5041 0.8177 0.5776 0.3047 0.2454 0.4439 0.4354 0.8606 0.64 0.4238 0.3318 0.5312 0.4359 0.8503 0.6884 0.5499 0.4098 0.7831
Cytoplasmic Foci 0.0257 0.0011 0.0045 0.0085 0.1034 0.0113 0.0446 0.0009 0.0052 0.0011 0.0137 0.0053 0.0384 0.0009 0.0039 0.0212 0.005 0.0028
Eisosomes 0.0009 0 0.0004 0.0003 0.0009 0.0005 0.0025 0 0.0016 0.0001 0.0005 0.0004 0.003 0.0003 0.0015 0.0001 0.0001 0.0006
Endoplasmic Reticulum 0.0122 0.0019 0.0052 0.0027 0.01 0.0046 0.0163 0.0024 0.0048 0.0085 0.0135 0.0223 0.0157 0.0014 0.008 0.0005 0.0029 0.0045
Endosome 0.0108 0.0021 0.0064 0.0137 0.1017 0.0392 0.0248 0.0021 0.0137 0.0057 0.0502 0.0767 0.0334 0.0011 0.0137 0.0214 0.0243 0.0041
Golgi 0.0018 0.0003 0.0024 0.0046 0.0108 0.054 0.0218 0.0003 0.0099 0.0002 0.0251 0.0179 0.0169 0.0003 0.0029 0.0236 0.0126 0.0014
Lipid Particles 0.009 0.0007 0.0031 0.048 0.0176 0.007 0.0147 0.0004 0.0039 0.0005 0.0115 0.004 0.0304 0.0008 0.0046 0.0321 0.0034 0.0026
Mitochondria 0.0223 0.0023 0.0335 0.09 0.1166 0.1182 0.0487 0.0018 0.0138 0.0039 0.0887 0.0262 0.0333 0.0049 0.0152 0.1096 0.0914 0.0518
None 0.0113 0.0005 0.0043 0.0058 0.0107 0.0023 0.01 0.0004 0.0062 0.0005 0.0026 0.0042 0.0231 0.0009 0.0116 0.0008 0.0013 0.0019
Nuclear Periphery 0.0296 0.004 0.0239 0.0136 0.0145 0.0169 0.0382 0.0036 0.0165 0.0157 0.0213 0.0512 0.0249 0.0043 0.0086 0.0083 0.0105 0.0297
Nucleolus 0.0111 0.0009 0.0177 0.011 0.0235 0.0136 0.0123 0.0004 0.0088 0.0015 0.0131 0.0034 0.0367 0.0048 0.0035 0.0043 0.0047 0.0057
Nucleus 0.0265 0.0055 0.0131 0.0155 0.0075 0.0339 0.0198 0.0054 0.0184 0.0129 0.0161 0.0177 0.0177 0.008 0.0139 0.0153 0.0152 0.0157
Peroxisomes 0.0027 0.0001 0.0007 0.043 0.009 0.0017 0.0038 0.0001 0.0011 0.0001 0.0047 0.0006 0.0271 0.0002 0.001 0.0127 0.0007 0.0013
Punctate Nuclear 0.0041 0 0.0003 0.0004 0.0024 0.0006 0.0017 0 0.0027 0.0001 0.0025 0.0013 0.0054 0 0.0009 0.0003 0.0002 0.0003
Vacuole 0.1722 0.1062 0.1831 0.2455 0.1981 0.0579 0.1171 0.0832 0.1286 0.2408 0.1768 0.116 0.1245 0.0779 0.126 0.1185 0.2689 0.0329
Vacuole Periphery 0.0643 0.0177 0.0456 0.1307 0.0777 0.0219 0.0335 0.0159 0.0421 0.1039 0.1012 0.0724 0.0324 0.0118 0.0347 0.0535 0.1154 0.0349

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2417.7042 2449.35 2972.5013 2592.1128 3036.2226 2225.8219 2276.003 2313.7435 2551.9739 2445.7053
Translational Efficiency 1.6629 1.6513 1.4868 1.6003 1.5608 1.9542 1.7243 1.8182 1.8235 1.779

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
213 493 770 104 227 1544 2290 1432 440 2037 3060 1536

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6658.95 10267.83 14017.26 12429.13 9145.03 10071.11 14365.20 10524.02 7941.54 10118.72 14277.65 10653.01
Standard Deviation 1529.02 2381.15 2535.69 2815.06 1730.88 2251.65 3193.13 2850.30 2054.50 2285.22 3044.86 2887.87
Intensity Change Log 2 — 0.624765 1.073838 0.900359 — 0.139163 0.651518 0.202626 — 0.363957 0.844712 0.538404

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000173 0.000120 0.000197 0.000078 0.000061 0.000129 0.000256 0.000106 0.000088 0.000127 0.000252
Bud Neck 0.000619 0.001209 0.002524 0.004020 0.000852 0.000395 0.000664 0.001294 0.000739 0.000592 0.001132 0.001479
Bud Site 0.005005 0.007733 0.004301 0.015867 0.004000 0.001765 0.004438 0.010108 0.004487 0.003210 0.004404 0.010498
Cell Periphery 0.041131 0.038110 0.020639 0.043218 0.043920 0.014844 0.009540 0.034791 0.042570 0.020475 0.012333 0.035362
Cytoplasm 0.459287* 0.585136* 0.513686* 0.279542* 0.504562* 0.767316* 0.683483* 0.611828* 0.482644* 0.723224* 0.640756* 0.589329*
Cytoplasmic Foci 0.013425 0.016030 0.017637 0.014177 0.014959 0.009281 0.005164 0.005681 0.014216 0.010914 0.008302 0.006256
Eisosomes 0.000003 0.000004 0.000005 0.000010 0.000003 0.000001 0.000002 0.000005 0.000003 0.000002 0.000003 0.000006
Endoplasmic Reticulum 0.000820 0.000897 0.000404 0.000444 0.000495 0.000234 0.000339 0.000386 0.000652 0.000395 0.000355 0.000390
Endosome 0.000890 0.001197 0.000640 0.001248 0.000820 0.000373 0.000507 0.001010 0.000854 0.000573 0.000540 0.001026
Golgi 0.131464* 0.096469 0.061543 0.035349 0.117812* 0.048396 0.015566 0.023227 0.124421* 0.060031 0.027135 0.024048
Lipid Particles 0.035100 0.028814 0.016077 0.019530 0.022846 0.010693 0.007804 0.020458 0.028778 0.015079 0.009886 0.020395
Mitochondria 0.000215 0.000300 0.000210 0.000177 0.000144 0.000136 0.000523 0.000691 0.000179 0.000175 0.000444 0.000656
Mitotic Spindle 0.001485 0.002833 0.001969 0.000586 0.001893 0.000785 0.000344 0.000425 0.001695 0.001281 0.000753 0.000436
None 0.002412 0.003438 0.002030 0.000708 0.001746 0.004546 0.001950 0.002849 0.002069 0.004278 0.001970 0.002704
Nuclear Periphery 0.000828 0.001614 0.002619 0.000910 0.003307 0.000753 0.000309 0.000394 0.002107 0.000961 0.000890 0.000429
Nuclear Periphery Foci 0.018172 0.019787 0.015021 0.005641 0.016787 0.012023 0.003549 0.005026 0.017457 0.013902 0.006436 0.005068
Nucleolus 0.001465 0.000256 0.000153 0.000287 0.000153 0.000143 0.000170 0.000192 0.000788 0.000170 0.000165 0.000199
Nucleus 0.008669 0.011731 0.020414 0.012427 0.008954 0.012842 0.009451 0.011666 0.008816 0.012573 0.012210 0.011717
Peroxisomes 0.000063 0.000044 0.000021 0.000074 0.000030 0.000009 0.000058 0.000091 0.000046 0.000018 0.000049 0.000090
Vacuole 0.147488 0.100102 0.217461* 0.477544* 0.123360 0.068908 0.193117 0.221641* 0.135040 0.076457 0.199243 0.238968*
Vacuole Periphery 0.131324* 0.084124 0.102526* 0.088045 0.133280* 0.046494 0.062895 0.047981 0.132333* 0.055601 0.072868 0.050694

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -0.24 -1.26 0.01 -1.26 0.93 -1.82 -5.43 -6.21 -2.62 0.27 -1.36 -4.78 -5.03 -3.05
Bud Neck -2.41 -8.05 -5.74 -4.64 -2.18 1.87 -0.14 -1.72 -4.50 -2.22 0.89 -4.20 -3.62 -4.65 -0.96
Bud Site -1.68 0.25 -1.64 -1.24 -1.69 1.91 -0.29 -3.87 -8.19 -5.36 1.55 -0.10 -4.85 -6.88 -5.64
Cell Periphery 0.59 5.21 0.35 -0.01 -2.51 6.31 7.67 2.14 -9.42 -13.61 7.08 10.26 2.56 -6.82 -12.57
Cytoplasm -4.16 -1.37 4.23 7.91 5.89 -10.19 -6.48 -3.89 13.09 5.39 -12.67 -7.76 -5.16 11.62 3.73
Cytoplasmic Foci -1.37 -3.03 -0.56 0.55 1.99 2.50 4.49 4.27 3.69 -0.54 2.02 3.45 5.35 5.15 3.23
Eisosomes -1.39 -3.87 -2.95 -2.62 -1.94 1.95 1.40 -2.17 -4.62 -4.08 1.73 0.19 -3.24 -4.66 -3.66
Endoplasmic Reticulum -0.53 2.86 2.10 2.45 0.05 1.73 1.11 0.77 -3.66 -1.22 2.49 3.07 2.69 0.40 -0.78
Endosome -1.96 2.15 -0.91 0.26 -1.83 2.75 1.91 -0.26 -1.80 -1.36 2.80 2.93 -0.31 -1.31 -1.38
Golgi 3.09 7.11 9.06 8.42 4.85 8.34 12.71 11.73 11.05 -4.81 9.68 15.44 15.85 15.24 2.38
Lipid Particles 1.10 4.00 3.08 2.56 -0.83 3.73 4.82 0.79 -5.81 -8.66 4.67 6.82 2.92 -3.00 -7.52
Mitochondria -0.80 -0.19 0.28 1.43 0.70 0.07 -3.46 -2.71 -2.90 -0.68 -0.03 -3.21 -2.59 -2.70 -1.00
Mitotic Spindle -2.11 -2.02 3.44 3.63 6.43 2.10 2.80 2.72 3.05 -0.84 1.28 2.83 3.99 4.77 5.13
None -2.11 0.93 4.66 5.53 2.91 -3.96 -0.67 -2.06 1.80 -1.43 -3.98 0.23 -1.34 1.97 -1.35
Nuclear Periphery -2.36 -8.44 -0.48 2.01 7.19 1.22 1.41 1.39 5.38 -1.44 1.04 1.05 1.54 5.50 8.05
Nuclear Periphery Foci -0.66 1.41 6.32 7.06 9.73 2.91 9.14 8.12 9.75 -3.77 2.44 8.91 10.19 11.95 4.18
Nucleolus 0.87 0.94 0.82 -0.47 -1.80 -0.05 -0.42 -0.66 -0.94 -0.81 0.90 0.90 0.85 -0.70 -1.47
Nucleus -1.13 -4.55 -1.71 -1.16 0.76 -2.12 -1.65 -2.19 -0.19 -0.87 -2.27 -3.59 -2.62 -0.64 1.13
Peroxisomes 0.65 1.49 -0.18 -1.25 -2.64 2.19 -2.15 -4.04 -6.81 -2.36 1.93 -0.09 -2.34 -6.30 -3.29
Vacuole 3.00 -4.92 -9.27 -11.21 -7.38 4.66 -6.81 -8.10 -20.77 -2.51 6.17 -7.74 -9.82 -22.16 -4.03
Vacuole Periphery 3.75 2.70 3.32 0.52 1.68 7.44 6.25 7.52 0.22 4.66 9.18 7.50 10.10 2.39 7.27
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps25a

Rps25a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps25a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available