Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 1.0 0.98 0.95 0.8 0.69 0.76 0.74 0.75 0.74 0.97 0.96 0.97 1.0 1.0 1.0 0.95 0.97 0.95 0.91 0.89 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.18 0.29 0.21 0.21 0.18 0.19 0 0 0 0.05 0 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 3 4 6 12 16 21 32 0 0 0 0 0 0 1 0 0 0 0 0
Bud 1 0 0 0 0 1 1 2 4 1 1 6 0 3 0 0 0 0 0 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 1 0 5 6 16 11 11 38 22 30 0 2 3 0 2 0 0 0 0 0 1 0
Cytoplasm 125 233 216 404 616 527 538 508 430 543 383 519 303 437 525 141 164 109 116 215 199 245 362 431
Endoplasmic Reticulum 0 0 1 2 7 19 123 214 117 151 90 132 9 15 16 7 4 4 1 1 5 5 22 16
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 4 3 8 15 5 17 2 0 1 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 1 0 0 5 5 4 7 7 13 28 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 4 0 0 1 1 0 0 1 0 0 1 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 3 5 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 0 2 3 2 2 3 1 1 2 2 0 0 0 0 1 0 7 7 5
Unique Cell Count 126 233 218 406 627 553 676 738 564 735 512 703 313 454 542 141 164 109 122 223 211 270 409 469
Labelled Cell Count 128 235 221 406 629 561 696 760 592 775 540 767 315 460 548 148 170 114 122 223 211 270 409 469


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 153.0 189.9 124.2 127.7 93.3 60.8 54.7 53.3 49.6 49.2 44.4 43.6 152.5 151.0 150.4 196.7 211.0 203.9 174.4 182.4 186.2
Std Deviation (1e-4) 40.0 33.8 29.8 29.1 20.6 13.6 12.7 13.1 11.2 11.7 11.2 10.5 35.6 34.4 34.3 53.4 58.7 49.2 45.4 49.0 43.7
Intensity Change (Log2) – – – 0.04 -0.41 -1.03 -1.18 -1.22 -1.32 -1.34 -1.49 -1.51 0.3 0.28 0.28 0.66 0.76 0.71 0.49 0.55 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 2.6 2.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 3.1 2.7 2.7 0 0 0 0 0 0
Cytoplasm 0.6 -0.9 -2.5 -6.9 -9.2 -7.6 -8.1 -7.8 -8.1 -1.7 -2.1 -1.8 1.1 1.2 1.0
Endoplasmic Reticulum 0 0 2.3 6.6 8.9 7.1 7.1 6.4 6.7 0 2.3 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 142.2067 186.3671 175.9856 164.3058 182.4247 173.8979 130.9823 175.8183 167.0233 148.358 122.002 145.2794 127.4352 195.2384 173.3512 159.9342 174.8711 176.1078
Actin 0.0013 0.0003 0.0004 0.0002 0.0005 0.0028 0.0119 0.0003 0.0005 0.0032 0.018 0.0034 0.033 0.0003 0.0097 0.0045 0.0002 0.002
Bud 0.0015 0.0007 0.0011 0.0012 0.0008 0.0017 0.0032 0.0008 0.0011 0.0032 0.0053 0.0056 0.0016 0.0007 0.0018 0.004 0.0008 0.0083
Bud Neck 0.0009 0.0006 0.001 0.0005 0.0016 0.0035 0.0008 0.0004 0.0011 0.001 0.0017 0.0018 0.0169 0.0004 0.0043 0.0008 0.0004 0.0011
Bud Periphery 0.0023 0.0007 0.001 0.0011 0.0003 0.0029 0.0035 0.0006 0.0007 0.0027 0.0058 0.0031 0.002 0.0003 0.0008 0.0029 0.0004 0.0024
Bud Site 0.0007 0.0005 0.0005 0.0004 0.0002 0.0002 0.0033 0.0005 0.0005 0.0009 0.002 0.001 0.0142 0.0009 0.0133 0.0032 0.0001 0.0005
Cell Periphery 0.0601 0.0441 0.0506 0.0384 0.0066 0.0156 0.0351 0.0082 0.0266 0.0078 0.0071 0.0109 0.051 0.0182 0.0192 0.0172 0.0087 0.0009
Cytoplasm 0.5544 0.8151 0.7064 0.4839 0.78 0.733 0.6531 0.9213 0.7899 0.5658 0.6667 0.6462 0.5169 0.9232 0.8311 0.626 0.9349 0.902
Cytoplasmic Foci 0.0065 0.0006 0.0027 0.0012 0.0032 0.0023 0.0267 0.0007 0.0102 0.004 0.0126 0.0055 0.02 0.0012 0.0091 0.0034 0.0018 0.0029
Eisosomes 0.001 0.0022 0 0 0 0.0001 0.0009 0 0 0.0001 0.0004 0.0001 0.0008 0.0002 0.0002 0.0002 0 0
Endoplasmic Reticulum 0.0644 0.0112 0.0286 0.0035 0.0124 0.0953 0.0228 0.004 0.0063 0.0037 0.0102 0.0481 0.0216 0.0022 0.0035 0.0021 0.001 0.0024
Endosome 0.0096 0.0014 0.0038 0.0052 0.0083 0.0108 0.0101 0.0015 0.0058 0.0212 0.0515 0.0226 0.0262 0.0006 0.0018 0.0156 0.0034 0.0091
Golgi 0.0022 0.0003 0.0005 0.0002 0.0003 0.0035 0.0038 0.0002 0.0008 0.0106 0.0248 0.0046 0.0091 0.0001 0.0015 0.0071 0.0001 0.0008
Lipid Particles 0.0063 0.0025 0.0026 0.0037 0.0007 0.0038 0.0114 0.0005 0.0021 0.0014 0.0044 0.0013 0.0182 0.0017 0.0027 0.0013 0.0004 0.0001
Mitochondria 0.0125 0.0008 0.0014 0.0016 0.0003 0.0098 0.0161 0.003 0.002 0.0212 0.0396 0.021 0.0206 0.0003 0.0027 0.015 0.0002 0.0083
None 0.0059 0.0004 0.0011 0.0005 0.0003 0.0007 0.0148 0.0002 0.0027 0.0014 0.0134 0.0024 0.019 0.0012 0.0035 0.0009 0.0005 0.0008
Nuclear Periphery 0.0378 0.0066 0.0091 0.0073 0.0188 0.0167 0.0233 0.0021 0.0086 0.0118 0.0313 0.0857 0.0424 0.004 0.0067 0.0147 0.0069 0.008
Nucleolus 0.0227 0.0005 0.0038 0.0026 0.0004 0.0005 0.0075 0.0001 0.0009 0.0025 0.0054 0.0091 0.0121 0.001 0.0044 0.0039 0.0002 0.0002
Nucleus 0.0131 0.0032 0.0049 0.0088 0.0122 0.0061 0.0134 0.0019 0.005 0.0077 0.0076 0.0178 0.0229 0.0034 0.0057 0.0133 0.0036 0.0086
Peroxisomes 0.0006 0 0.0001 0.0001 0 0.0001 0.0014 0 0.0003 0.0006 0.0014 0.0007 0.0108 0 0.0061 0.0005 0 0.0003
Punctate Nuclear 0.0011 0 0.0001 0.0001 0.0001 0.0002 0.0024 0 0.0007 0.0002 0.0011 0.0011 0.0079 0.0001 0.0003 0.0002 0.0001 0.0001
Vacuole 0.171 0.1019 0.1613 0.3688 0.1308 0.0769 0.1092 0.0484 0.1174 0.2467 0.0688 0.0666 0.1066 0.0375 0.0619 0.1954 0.0289 0.0261
Vacuole Periphery 0.0239 0.0062 0.0191 0.0707 0.0221 0.0138 0.0254 0.0053 0.0168 0.0824 0.0209 0.0414 0.0263 0.0028 0.0098 0.0682 0.0077 0.0151

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1958.2555 2149.9918 2243.453 2062.0054 2412.2951 1898.2984 1733.6295 1582.0224 1728.0096 1777.1372
Translational Efficiency 1.5698 1.4434 1.5455 1.2897 1.643 1.6888 1.5459 1.7532 1.6207 1.7016

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl26b

Rpl26b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl26b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available