Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.06 0.06 0.07 0.06 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.1 0.12 0.09 0.11 0 0.07 0.05 0.06 0.05 0 0.15 0.21 0.24 0.09 0.17 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.33 0.42 0.28 0.47 0.63 0.58 0.7 0.7 0.67 0.75 0 0 0.07 0 0 0.07 0 0 0 0 0 0
Nucleus 0.8 0.66 0.61 0.68 0.66 0.63 0.59 0.6 0.6 0.58 0.55 0.48 0.78 0.71 0.67 0.78 0.7 0.72 0.7 0.67 0.53 0.63 0.5 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.06 0.05 0.12 0.16 0.18 0.17 0.18 0.19 0 0 0 0 0 0 0.06 0 0.05 0.06 0.11 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.05 0.07 0.07
Vac/Vac Membrane 0.17 0.23 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.14 0.16 0.09 0.14 0.19 0.14 0.16 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2
Bud 0 0 1 3 3 3 6 9 4 7 14 4 0 0 2 0 0 0 1 3 4 5 4 9
Bud Neck 0 0 2 0 1 0 2 2 0 1 2 0 0 0 0 0 0 0 0 2 1 1 2 3
Bud Site 0 0 2 3 8 9 18 28 16 27 14 18 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 0 0 0 0 0 0 0 0 0
Cytoplasm 1 29 34 24 30 33 18 33 14 23 13 14 34 97 109 17 23 17 2 13 18 7 2 6
Endoplasmic Reticulum 0 2 2 0 0 0 0 0 0 0 0 0 1 0 0 12 4 5 0 1 4 2 1 4
Endosome 1 11 3 0 12 1 0 0 0 0 0 0 2 17 8 7 6 4 4 8 9 5 21 44
Golgi 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 3 0 2 0 1
Mitochondria 2 23 109 83 92 137 255 283 197 261 163 243 7 5 30 4 1 10 1 8 14 0 9 18
Nucleus 98 218 198 134 217 183 240 292 170 214 134 156 177 325 307 152 97 110 111 263 228 141 156 184
Nuclear Periphery 0 1 0 0 0 1 0 1 1 2 5 5 0 0 0 0 0 1 0 1 1 0 0 6
Nucleolus 3 3 1 3 18 15 48 79 51 63 44 62 4 12 15 1 0 2 9 16 21 12 35 36
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 7 5 17 6 15 18 13 6 3 6 3 16 12 0 0 3 7 12 28 10 21 31
Vac/Vac Membrane 21 76 20 2 11 6 4 5 2 1 4 2 6 13 10 25 20 24 14 54 83 30 50 90
Unique Cell Count 123 330 326 196 327 290 404 487 282 372 242 322 227 456 457 195 139 153 158 395 429 225 315 461
Labelled Cell Count 129 365 379 258 409 394 606 751 468 605 396 510 234 488 501 218 153 177 158 395 429 225 315 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.3 4.3 4.4 4.6 4.3 3.8 3.9 3.7 3.7 3.8 3.5 5.8 5.8 5.2 8.2 9.2 8.7 5.7 6.2 6.6
Std Deviation (1e-4) 1.1 1.1 1.0 1.5 1.6 1.8 1.2 1.3 1.2 1.4 1.2 1.1 1.2 1.3 1.2 1.3 1.9 1.6 1.5 1.8 1.8
Intensity Change (Log2) – – – 0.0 0.09 -0.01 -0.18 -0.14 -0.21 -0.22 -0.19 -0.3 0.41 0.43 0.26 0.91 1.09 1.01 0.4 0.51 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -0.5 0.4 -3.1 -1.9 -2.5 -2.0 -2.2 -3.0 1.6 4.0 4.8 -0.6 1.8 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.8 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -8.6 -12.6 -9.7 -8.4 -7.6 -6.4
Nucleus 1.8 1.5 0.6 -0.4 -0.2 -0.1 -0.9 -1.3 -3.1 4.3 3.1 1.9 4.0 1.9 2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 2.5 2.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.1 0.4 0 0 0
Vacuole -2.8 -1.7 -2.5 -3.9 -4.1 -3.6 -4.5 -2.6 -3.9 -1.9 -2.3 -2.8 2.6 2.9 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7614 2.4163 2.3254 2.0075 1.9527 2.0827 0.3124 0.8096 0.7562 0.3185 0.5264 0.6374 5.2428 5.4428 5.274 5.8953 4.9385 5.3961
Actin 0.0275 0.001 0.0107 0.0021 0.0147 0.0021 0.0008 0 0 0 0 0.0002 0.0002 0 0.005 0.0002 0.0013 0
Bud 0.0006 0.0004 0.0007 0.0002 0.0007 0.0001 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0053 0.0008 0.0024 0.0008 0.0076 0.0038 0.0002 0.0002 0.0002 0.0001 0.0006 0.0014 0.0043 0.0003 0.0004 0.0003 0.0027 0.0016
Bud Periphery 0.0016 0.001 0.0017 0.0009 0.001 0.0002 0.0001 0 0 0 0 0 0.0006 0 0.0001 0 0.0001 0
Bud Site 0.0048 0.0011 0.0083 0.0004 0.0096 0.0003 0 0.0001 0.0001 0 0 0.0001 0.0002 0.0003 0.0006 0.0001 0.0003 0.0001
Cell Periphery 0.0005 0.0001 0.0009 0.0002 0.0002 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0048 0.0112 0.0032 0.0158 0.0084 0.0013 0.0008 0.0044 0.0007 0.0002 0.0021 0.0014 0.0043 0.0105 0.0053 0.0118 0.0088 0.0035
Cytoplasmic Foci 0.0129 0.0004 0.0044 0.0006 0.0076 0.001 0.0002 0.0001 0.0001 0 0 0.0007 0.001 0.0003 0.0004 0.0003 0.003 0.0001
Eisosomes 0.0003 0 0.0002 0.0001 0.0002 0 0 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.007 0.0014 0.002 0.0015 0.002 0.0003 0.0002 0.0002 0 0 0.0001 0.0001 0.0003 0.0005 0.0002 0.0006 0.0003 0.0002
Endosome 0.0156 0.0016 0.0253 0.002 0.0164 0.0041 0.0005 0.0004 0.0001 0 0.0001 0.0004 0.0009 0.001 0.0003 0.0018 0.0091 0.0002
Golgi 0.0111 0.0002 0.0071 0.0014 0.0169 0.0021 0.0001 0 0 0 0 0.0001 0.0001 0 0.0002 0 0.0076 0
Lipid Particles 0.0092 0.0002 0.0039 0.0027 0.0187 0.0017 0.0004 0 0 0 0 0.0001 0.0008 0.0002 0.0004 0.0002 0.032 0
Mitochondria 0.0083 0.0012 0.0142 0.0614 0.018 0.0107 0.0006 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0006 0.0017 0.0001
None 0.007 0.0066 0.0007 0.0018 0.0009 0.0003 0.0005 0.0009 0.0002 0.0001 0.0002 0.0003 0.0139 0.0044 0.0007 0.0169 0.0607 0.0028
Nuclear Periphery 0.0228 0.0125 0.014 0.0045 0.0106 0.0024 0.0032 0.0124 0.002 0.0005 0.0036 0.002 0.0016 0.0063 0.0036 0.0078 0.002 0.0015
Nucleolus 0.0251 0.0151 0.0192 0.0166 0.0249 0.0316 0.0107 0.0113 0.0145 0.0041 0.0137 0.0183 0.0135 0.0117 0.0203 0.0097 0.0394 0.0247
Nucleus 0.7971 0.9231 0.8423 0.8502 0.7997 0.9124 0.9655 0.9553 0.9609 0.9682 0.9713 0.9608 0.9324 0.9523 0.9316 0.9361 0.8139 0.9331
Peroxisomes 0.0107 0.0001 0.0016 0.0089 0.0093 0.0004 0.0001 0 0 0 0 0.0001 0.0002 0 0.0023 0.0001 0.002 0
Punctate Nuclear 0.0228 0.0196 0.0284 0.0242 0.03 0.0244 0.0156 0.0142 0.0209 0.0268 0.0082 0.0137 0.0248 0.0112 0.028 0.0124 0.0143 0.0319
Vacuole 0.003 0.0019 0.0064 0.0018 0.0014 0.0004 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0005 0.0006 0.0003 0.0009 0.0003 0.0002
Vacuole Periphery 0.0019 0.0004 0.0024 0.0018 0.0009 0.0003 0.0002 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0.0002 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6223 13.7798 12.1276 14.1708 12.0844 13.2565 13.8517 11.6999 15.4679 16.5048
Translational Efficiency 0.9389 0.8675 0.7973 0.7881 0.8811 0.845 0.8507 0.7939 0.6361 0.7021

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
Localization
Cell Percentages nucleus (81%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tfc4

Tfc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available