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Basic protein informationORFYGR060W
Common nameERG25
Alias
DescriptionC-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
Micrographs
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Wild type 1 (WT1)
Wild type 2 (WT2)
Wild type 3 (WT3)

ORF-GFP <-> cytosolic RFP <-> ORF-GFP/cytosolic RFP overlay        
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Sub-cellular localization
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ensLOCDeepLoc
LocalizationWT1WT2WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3WT1WT2WT3AF100AF140AF180
Cort. Patches000000000000000000000000
Bud000000000000000000000000
Budneck000000000000000000000000
Budsite000000000000000000000000
Cell Periphery00000.05.07.05.07.06.08.09000000000000
Cytoplasm.68.49.25.5.43.59.51.53.66.65.7.65.28.2.41.47.44.51.12.130000
Endosome000000000000000000000000
ER.16.45.65.50.56.41.48.46.31.31.28.30.64.63.4800.05.45.56.81.51.50.44
Golgi000000000000000000000000
Mito000000000000000000000000
Nuc Periphery000000000000000000000000
Nucleus000000000000000000000000
Nucleolus000000000000000000000000
Peroxisome000000000000000000000000
SpindlePole000000000000000000000000
Vac/Vac Memb.19.07.13000000000.09.21.12.55.57.47.32.22.15.43.4.43
TotalCells6929324322635834954349843552638346819017519217316014796411286157263237

Cell count <-> LOC-score        Download localization matrix
YGFP assignment in Huh et al. (2003) ER, VACUOLE
Protein Abundance
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ScreenWT1WT2WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3AF100AF140AF180
Mean GFP Intensity Ig(x 1e-4)53.363.849.851.455.346.646.646.448.945.646.245.248.636.634.541.141.642.154.552.051.0
Std Deviation (x 1e-4)24.717.611.411.011.29.39.111.010.710.310.610.311.59.68.712.113.212.814.515.619.0
Abundance Change δPL (Log2)nanana0.00.2-0.1-0.1-0.1-0.0-0.1-0.1-0.1-0.0-0.4-0.5-0.3-0.3-0.20.10.10.0

Plot time-course intensity of:   rapamycin  alpha-factor  hydroxy-urea
Sub-cellular localization change
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Localization/ScreenRAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700HU80HU120HU160rpd3Δ_1rpd3Δ_2rpd3Δ_3
Cort. Patches000000000000000
Bud000000000000000
Budneck000000000000000
Budsite000000000000000
Cell Periphery03.23.64.23.54.33.84.54.8000000
Cytoplasm5.44.57.96.57.110.210.310.99.90.7-1.23.64.53.75.0
Endosome000000000000000
ER-3.3-2.0-5.6-4.4-4.8-8.5-8.7-9.1-8.9-0.1-0.2-3.5-13.3-12.5-11.6
Golgi000000000000000
Mito000000000000000
Nuc Periphery000000000000000
Nucleus000000000000000
Nucleolus000000000000000
Peroxisome000000000000000
SpindlePole000000000000000
Vac/Vac Memb-3.9-5.7-6.0-5.4-6.2-5.1-4.3-5.5-5.9-1.42.0-0.49.19.37.4

Download z-score matrix



Copyright © Moffat Lab and Andrews lab at CCBR, University of Toronto, 2014