Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.1 0.08 0.19 0.13 0.11 0.13 0.12 0.07 – 0.07 0 0.08 0.16 0.19 0.09 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.05 0 0.06 0.07 0.11 0.09 0.08 0.05 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.43 0.08 0.22 0.21 0.48 0.47 0.31 0.67 – 0.74 0.49 0.24 0 0.12 0.08 0.05 0.14 0 0 0.05 0.06 0 0.05
Nucleus 0.52 0.53 0.65 0.52 0.6 0.53 0.57 0.6 0.56 – 0.57 0.68 0.68 0.67 0.59 0.76 0.74 0.7 0.32 0.36 0.29 0.37 0.33 0.27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0.06 0.06 0 0 0.19 0.12 0.15 0.13 0.14 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.05 0.19 0.2 0.12 0.1 0.1 0.14 0 – 0.05 0.11 0 0 0 0 0 0.05 0.16 0.09 0.14 0.11 0.12 0.14
Vac/Vac Membrane 0.05 0.08 0 0 0.07 0 0 0 0 – 0 0 0 0.05 0.06 0 0.09 0.06 0.09 0.17 0.17 0.17 0.17 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 0 3 2
Bud 1 0 0 3 4 1 3 5 3 – 7 6 0 0 2 2 0 0 0 0 1 3 9 14
Bud Neck 0 0 10 4 4 2 5 16 5 – 5 7 0 0 0 0 0 0 1 2 2 3 7 12
Bud Site 0 0 0 0 0 4 4 3 11 – 11 7 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 8 12 32 25 30 43 43 23 – 19 12 18 42 60 22 11 15 0 5 6 3 4 11
Endoplasmic Reticulum 2 1 0 0 0 0 1 1 0 – 0 0 0 0 0 9 6 8 1 0 0 1 0 5
Endosome 6 1 4 7 7 9 1 3 3 – 1 2 7 20 15 8 11 11 20 11 17 7 26 34
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 4 1 1 0 0 1 1
Mitochondria 57 36 11 37 40 125 159 115 212 – 205 136 55 12 37 19 10 22 4 4 11 9 13 23
Nucleus 69 44 93 86 115 138 193 222 178 – 158 188 155 180 189 193 140 112 60 47 67 57 115 116
Nuclear Periphery 1 1 0 0 0 1 0 0 2 – 3 0 1 0 1 3 0 0 3 3 5 1 4 4
Nucleolus 9 2 0 0 0 0 2 3 13 – 11 4 12 9 20 15 7 2 36 16 35 19 51 63
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 1
SpindlePole 2 4 27 33 23 26 34 52 14 – 15 31 9 8 7 11 7 8 29 11 31 16 44 62
Vac/Vac Membrane 6 7 2 3 14 8 4 5 9 – 3 6 6 13 20 11 17 10 16 21 38 26 60 55
Unique Cell Count 133 83 143 166 191 262 340 372 318 276 275 229 268 319 253 189 159 188 131 231 156 355 434
Labelled Cell Count 159 104 159 205 232 344 449 469 473 438 400 264 285 352 297 211 192 188 131 231 156 355 434


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 4.9 4.7 5.1 3.9 4.2 4.4 3.7 – 3.6 4.0 4.7 4.9 4.8 7.3 7.1 7.4 4.4 4.6 4.8
Std Deviation (1e-4) 0.8 0.8 0.9 1.6 1.5 0.9 1.2 0.9 1.2 – 1.2 0.8 1.1 1.2 1.3 1.4 1.1 1.8 1.4 1.7 1.9
Intensity Change (Log2) – – – -0.06 0.06 -0.3 -0.2 -0.14 -0.39 – -0.45 -0.28 -0.06 0.0 -0.01 0.6 0.56 0.6 -0.15 -0.09 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.0 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8199 1.9261 1.7328 1.6858 1.3279 1.9263 -0.0656 -0.0923 -0.3914 -0.2401 -0.2088 -0.3257 -0.1268 -0.1069 -0.2661 0.121 -0.484 -0.1912
Actin 0.0235 0.0033 0.0073 0.0014 0.0013 0.0019 0.0157 0.0006 0.004 0.0066 0.0028 0.001 0.0007 0.0001 0.0006 0.0003 0.0001 0.0001
Bud 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0009 0.0001 0.0001 0.0001 0 0 0 0
Bud Neck 0.0114 0.0013 0.0014 0.0017 0.0032 0.0152 0.0114 0.0016 0.0009 0.001 0.0032 0.006 0.0008 0.0007 0.0007 0.001 0.0008 0.0026
Bud Periphery 0.0004 0.0002 0.0002 0.0001 0.0002 0.0005 0.0009 0.0004 0.0006 0.0001 0.0018 0.0001 0.0001 0 0.0001 0 0 0
Bud Site 0.0098 0.0016 0.0008 0.0005 0.0004 0.0007 0.0018 0.0008 0.0014 0.0005 0.0119 0.0001 0.0003 0.0004 0.0002 0.0001 0 0
Cell Periphery 0.0004 0.0001 0.0001 0.0001 0.0001 0.0007 0.0002 0.0009 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.0341 0.0178 0.0104 0.0324 0.0109 0.0189 0.0097 0.0091 0.0083 0.004 0.0079 0.0021 0.0046 0.0049 0.0051 0.0032 0.0011 0.0021
Cytoplasmic Foci 0.0167 0.0063 0.0065 0.0029 0.0071 0.0072 0.0128 0.0003 0.003 0.0086 0.0077 0.0017 0.0033 0.0003 0.0004 0.0003 0.0002 0.0005
Eisosomes 0.0015 0.0002 0.0007 0.0001 0.0001 0.0003 0.0003 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.005 0.0017 0.001 0.0031 0.0009 0.0011 0.0022 0.001 0.0008 0.0007 0.001 0.0001 0.0008 0.0002 0.0004 0.0002 0.0001 0.0001
Endosome 0.0163 0.005 0.0065 0.0038 0.0077 0.0036 0.0157 0.0019 0.0072 0.0097 0.0258 0.0021 0.0017 0.0002 0.0008 0.0004 0.0003 0.0002
Golgi 0.0052 0.001 0.0024 0.0002 0.002 0.003 0.0081 0.0002 0.0018 0.0043 0.0373 0.0016 0.0002 0 0.0002 0 0 0
Lipid Particles 0.0204 0.009 0.0083 0.0005 0.0267 0.0066 0.0326 0.0005 0.005 0.0033 0.0252 0.0032 0.0011 0 0.0007 0.0001 0 0.0001
Mitochondria 0.0119 0.0017 0.0111 0.0008 0.0026 0.0104 0.0284 0.0013 0.0029 0.0008 0.0191 0.0046 0.0022 0.0001 0.0014 0.0002 0 0
None 0.0923 0.0939 0.0829 0.0521 0.056 0.0828 0.0047 0.014 0.0128 0.0032 0.0029 0.0009 0.012 0.0123 0.006 0.0021 0.0004 0.0024
Nuclear Periphery 0.0385 0.0346 0.0671 0.0309 0.0393 0.0041 0.0289 0.0951 0.0671 0.0166 0.0422 0.0113 0.0153 0.0105 0.0193 0.0282 0.0355 0.0039
Nucleolus 0.0389 0.0443 0.0427 0.0331 0.0673 0.0586 0.037 0.0487 0.0438 0.0403 0.0375 0.0756 0.0254 0.026 0.0314 0.0222 0.0212 0.0428
Nucleus 0.5331 0.6491 0.6039 0.5473 0.5754 0.6985 0.7001 0.7304 0.7464 0.8465 0.6322 0.8336 0.6695 0.6053 0.5943 0.4941 0.5598 0.6202
Peroxisomes 0.0173 0.0065 0.0109 0.0003 0.0173 0.004 0.0216 0.0001 0.0039 0.0009 0.0056 0.0017 0.0002 0 0.0002 0 0 0
Punctate Nuclear 0.1199 0.12 0.1334 0.2874 0.1795 0.08 0.0637 0.0841 0.0872 0.0518 0.1261 0.0497 0.2601 0.3386 0.3378 0.4473 0.3801 0.3249
Vacuole 0.0023 0.0014 0.0009 0.0007 0.0009 0.0013 0.0022 0.0073 0.0015 0.0004 0.0073 0.0034 0.0007 0.0001 0.0002 0.0002 0.0001 0
Vacuole Periphery 0.0007 0.0009 0.0013 0.0003 0.001 0.0003 0.0014 0.0016 0.001 0.0003 0.0011 0.0011 0.0006 0.0001 0.0002 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.8154 5.3462 4.5339 9.0573 8.8617 8.1119 8.6875 8.7301 7.6177 11.8097
Translational Efficiency 0.3537 0.8267 0.9361 0.3731 0.433 0.4996 0.5692 0.3817 0.5696 0.3778

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (79%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Env11

Env11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Env11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available