Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – – 0 0.05 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 – – 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0.09 0.23 0.2 0.19 0 0 0.08 0 0 0 0 0.21 0.39 0.43 0.62 – – 0 0.08 0 0.06 0.07 0.08
Mitochondria 0.96 0.87 0.87 0.93 0.79 0.88 0.9 0.71 0.92 0.9 0.91 0.87 0.87 0.75 0.76 0 – – 0.85 0.76 0.79 0.76 0.72 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0 0.05 0.06 0.12 0.08 0 0.12 0.07 0.13 0.07 0 0.05 0 0 – – 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0.07 0.1 0.11 0.09 0.12 0.12 0.11 0.13 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0.07 0.06 0.05 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0.05 0.16 0.05 0.05 0.15 0 0 0 0 0.06 0.11 0.1 0.16 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 4 3 6 0 3 2 1 1 1 0 9 13 3 12 – – 3 7 4 6 13 19
Bud 0 1 3 2 3 1 7 6 3 5 5 7 2 4 3 2 – – 0 1 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – 0 0 0 0 1 0
Bud Site 0 0 1 0 0 0 2 2 3 0 2 3 0 0 0 0 – – – – – – – –
Cell Periphery 0 4 4 3 1 2 2 0 0 1 0 2 10 9 13 5 – – 1 0 0 0 0 0
Cytoplasm 0 13 3 3 2 4 6 4 3 5 3 3 4 9 10 4 – – 0 5 0 0 0 0
Endoplasmic Reticulum 0 1 2 0 3 3 1 0 0 0 3 1 2 0 2 1 – – 0 0 0 0 0 1
Endosome 0 0 2 4 4 4 5 7 0 1 0 2 4 3 5 1 – – 2 0 5 1 7 8
Golgi 2 14 43 33 42 2 0 14 1 3 3 0 46 120 127 60 – – 8 12 9 10 22 24
Mitochondria 216 134 162 155 175 192 250 125 158 208 236 222 189 231 225 4 – – 191 122 162 134 217 218
Nucleus 0 1 0 1 0 0 5 1 1 0 5 1 1 0 0 1 – – 0 1 0 0 0 0
Nuclear Periphery 19 4 7 8 13 27 21 6 21 16 34 19 9 15 10 3 – – 2 0 2 0 1 3
Nucleolus 17 3 6 5 16 22 31 16 20 28 29 34 3 8 2 1 – – 0 0 0 3 4 3
Peroxisomes 0 2 8 1 0 0 2 1 1 0 1 0 9 5 10 4 – – 11 4 15 11 15 21
SpindlePole 1 0 0 0 2 1 4 6 0 4 1 3 0 1 0 2 – – 1 1 0 2 2 0
Vac/Vac Membrane 11 3 5 8 35 10 14 27 5 8 8 10 12 33 30 16 – – 3 1 1 4 9 10
Unique Cell Count 224 154 187 167 222 219 278 177 172 230 260 254 217 306 297 97 227 161 205 177 304 317
Labelled Cell Count 266 183 250 226 302 268 353 217 217 281 331 307 300 451 440 116 227 161 205 177 304 317


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.8 18.2 12.9 11.8 11.9 10.1 9.3 9.3 8.1 8.0 7.8 8.0 13.1 14.2 13.5 17.9 – – 15.4 14.7 16.3
Std Deviation (1e-4) 2.7 5.1 2.7 2.0 2.3 2.6 2.2 2.6 2.3 1.9 2.1 1.9 2.6 2.4 2.5 4.4 – – 3.5 3.9 3.9
Intensity Change (Log2) – – – -0.14 -0.12 -0.36 -0.48 -0.47 -0.67 -0.7 -0.74 -0.69 0.01 0.14 0.06 0.47 – – 0.25 0.18 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 1.1 1.2 0 3.5 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1.4 0 1.3 0 – –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 1.2 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi -0.7 -1.0 -7.1 -8.4 -4.0 -6.5 -7.0 -7.5 -8.0 -0.4 3.7 4.4 6.5 – –
Mitochondria 1.9 -2.1 0.3 1.1 -3.7 1.6 1.2 1.4 0.2 0.1 -3.0 -2.9 -13.4 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0.5 1.0 3.1 1.7 -0.2 3.0 1.4 3.4 1.6 0.2 0.6 -0.2 0 – –
Nucleolus -0.1 1.8 2.7 3.1 2.3 3.1 3.3 3.1 3.7 0 -0.4 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 -0.1 0 -0.5 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 1.1 4.4 1.0 1.3 4.2 0 0.5 0.3 0.7 1.4 3.3 3.1 4.2 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5857 11.1681 11.0279 10.4292 9.2521 10.5055 10.6865 11.8067 10.8038 9.3004 10.535 10.9646 13.8153 12.9552 12.5854 12.9843 12.8224 13.1547
Actin 0.0018 0.0027 0.0021 0.0004 0.0002 0.0004 0.0078 0.0067 0.0059 0.1001 0.0287 0.0002 0.0151 0.0018 0.0052 0.002 0.0001 0.0006
Bud 0.0001 0 0 0 0.0001 0 0.0004 0.0001 0.0002 0.0009 0.0003 0 0.0004 0.0001 0.0003 0.0003 0 0
Bud Neck 0 0 0 0 0.0002 0 0.0004 0.0002 0.0001 0.0012 0.0031 0 0.0029 0 0.0001 0 0 0
Bud Periphery 0.0002 0.0001 0 0 0.0003 0 0.0008 0.0002 0.0011 0.0013 0.0006 0 0.0005 0.0001 0.0006 0.0013 0 0.0001
Bud Site 0.0003 0.0001 0 0.0001 0.0001 0 0.0015 0.0009 0.0008 0.0028 0.0016 0 0.0048 0.0001 0.0005 0.0001 0 0
Cell Periphery 0.0001 0.0001 0 0 0.0006 0 0.0004 0.0002 0.0019 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0001 0 0.0001
Cytoplasm 0.0014 0 0.0001 0.0001 0 0 0.0012 0 0.0002 0.0017 0.0007 0 0.0022 0 0.0006 0 0 0
Cytoplasmic Foci 0.0035 0.0008 0.0029 0.0071 0.0004 0.0007 0.0109 0.0015 0.0011 0.0063 0.0057 0.0002 0.0039 0.0011 0.0016 0.0002 0 0.0008
Eisosomes 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0003 0.0004 0.0005 0.0004 0.0001 0.0003 0.0002 0.0002 0.0002 0 0.0002
Endoplasmic Reticulum 0.0002 0 0.0001 0 0.0001 0 0.0016 0 0.0003 0.002 0.0008 0 0.0007 0 0.0005 0 0 0.0001
Endosome 0.0062 0.0031 0.0011 0.0006 0.0034 0.0028 0.0105 0.0023 0.002 0.0095 0.0097 0.0044 0.0026 0.0036 0.0021 0.001 0.0001 0.003
Golgi 0.0111 0.0143 0.0058 0.0007 0.0018 0.0119 0.0042 0.009 0.0041 0.0081 0.004 0.0102 0.0088 0.0236 0.0076 0.0036 0.0001 0.0103
Lipid Particles 0.0086 0.0032 0.0038 0.009 0.0114 0.0065 0.0283 0.0163 0.007 0.008 0.009 0.0031 0.0063 0.0023 0.0026 0.0009 0.0004 0.0038
Mitochondria 0.9493 0.9624 0.9738 0.9734 0.9572 0.9729 0.8857 0.946 0.9696 0.8274 0.8825 0.9754 0.927 0.9518 0.961 0.9864 0.9983 0.9719
None 0.0001 0 0.0001 0.0007 0 0 0.0026 0 0.0003 0.003 0.0014 0 0.0005 0 0.0005 0 0 0.0001
Nuclear Periphery 0.0025 0.0001 0.0001 0.0001 0.0001 0 0.0071 0.0005 0.0002 0.0071 0.0068 0 0.0012 0 0.0044 0 0 0.0006
Nucleolus 0.0007 0.0005 0 0.0004 0.0004 0.0001 0.0039 0.0004 0.0001 0.0006 0.0033 0 0.0041 0.0001 0.0012 0 0 0.0001
Nucleus 0.001 0.0039 0 0.0001 0.0002 0 0.003 0.0001 0.0001 0.0022 0.0039 0 0.0015 0 0.0021 0 0 0.0001
Peroxisomes 0.0026 0.0017 0.0078 0.0047 0.009 0.0008 0.0116 0.0079 0.0036 0.0102 0.0041 0.0004 0.0034 0.0037 0.0017 0.0018 0.0002 0.001
Punctate Nuclear 0.0006 0.0018 0.0003 0.0008 0 0 0.003 0 0.0003 0.0055 0.0313 0 0.0011 0 0.0008 0 0 0.0041
Vacuole 0.0016 0.001 0.0002 0.0003 0.0095 0.0004 0.0067 0.002 0.0003 0.0006 0.0006 0.0006 0.0025 0.0029 0.0008 0.0003 0.0001 0.0003
Vacuole Periphery 0.0078 0.004 0.0015 0.0012 0.0049 0.0031 0.008 0.005 0.0006 0.0009 0.0013 0.0051 0.0098 0.0084 0.0054 0.0019 0.0006 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.931 12.5715 17.9396 24.4912 17.532 20.5422 26.6442 28.0469 26.697 28.5836
Translational Efficiency 1.3667 2.2355 1.2527 0.9167 1.4429 1.3736 1.3616 1.0435 1.1879 1.0693

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
312 1208 658 1175 1429 1373 194 1532 1741 2581 852 2707

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1125.75 1423.39 1533.38 1687.54 1215.82 1338.08 1660.33 1759.08 1199.68 1378.01 1562.29 1728.03
Standard Deviation 225.69 283.05 272.63 337.06 232.35 252.98 287.54 328.41 233.73 270.84 281.18 334.08
Intensity Change Log 2 — 0.338445 0.445829 0.584035 — 0.138235 0.449540 0.532891 — 0.237960 0.447757 0.557706

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002286 0.003922 0.003426 0.008884 0.001364 0.003987 0.007392 0.004417 0.001529 0.003956 0.004329 0.006356
Bud Neck 0.004162 0.000965 0.005011 0.001525 0.001166 0.000704 0.004816 0.002096 0.001703 0.000826 0.004967 0.001848
Bud Site 0.003803 0.000721 0.004730 0.002570 0.000406 0.001298 0.002722 0.004706 0.001015 0.001028 0.004273 0.003778
Cell Periphery 0.000222 0.000070 0.000124 0.001205 0.000035 0.000274 0.000114 0.000907 0.000068 0.000179 0.000121 0.001036
Cytoplasm 0.000556 0.000464 0.000418 0.000267 0.000143 0.000319 0.000461 0.000422 0.000217 0.000387 0.000428 0.000355
Cytoplasmic Foci 0.016155 0.017284 0.026470 0.005569 0.014265 0.011391 0.033643 0.007741 0.014604 0.014149 0.028103 0.006798
Eisosomes 0.000622 0.000724 0.000269 0.001777 0.000509 0.000450 0.000722 0.001006 0.000529 0.000578 0.000372 0.001341
Endoplasmic Reticulum 0.000212 0.000154 0.000211 0.000186 0.000088 0.000136 0.000221 0.000707 0.000111 0.000145 0.000213 0.000481
Endosome 0.005949 0.003129 0.009780 0.001562 0.002203 0.003671 0.004702 0.004097 0.002875 0.003418 0.008624 0.002996
Golgi 0.014414 0.026285 0.027525 0.018195 0.015504 0.027470 0.025504 0.032650 0.015308 0.026916 0.027065 0.026376
Lipid Particles 0.004828 0.006352 0.009720 0.002482 0.006229 0.003234 0.007628 0.005655 0.005978 0.004693 0.009244 0.004278
Mitochondria 0.900399* 0.914523* 0.855680* 0.928995* 0.935336* 0.925761* 0.829889* 0.885212* 0.929075* 0.920501* 0.849808* 0.904216*
Mitotic Spindle 0.000795 0.002250 0.006988 0.001796 0.000937 0.000486 0.010574 0.003296 0.000912 0.001312 0.007804 0.002645
None 0.001912 0.001393 0.002186 0.002358 0.001716 0.001288 0.002016 0.002790 0.001751 0.001337 0.002148 0.002603
Nuclear Periphery 0.000387 0.000178 0.002554 0.000228 0.000456 0.000147 0.001507 0.000967 0.000444 0.000162 0.002315 0.000646
Nuclear Periphery Foci 0.000816 0.000184 0.001429 0.000104 0.000401 0.000124 0.000799 0.000339 0.000475 0.000152 0.001286 0.000237
Nucleolus 0.001076 0.000325 0.001490 0.000246 0.000306 0.000246 0.000971 0.000490 0.000444 0.000283 0.001372 0.000384
Nucleus 0.001936 0.000295 0.000242 0.000053 0.000064 0.000579 0.000094 0.000143 0.000399 0.000446 0.000208 0.000104
Peroxisomes 0.025272 0.013301 0.012144 0.009112 0.012899 0.011406 0.045566 0.002906 0.015116 0.012293 0.019754 0.005600
Vacuole 0.009062 0.003644 0.013410 0.004027 0.002988 0.003218 0.008749 0.014408 0.004077 0.003418 0.012349 0.009902
Vacuole Periphery 0.005136 0.003836 0.016192 0.008860 0.002984 0.003809 0.011909 0.025045 0.003370 0.003822 0.015217 0.018020

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.68 -1.16 -4.41 -3.36 -3.73 -3.60 -3.17 -4.42 -0.47 1.47 -4.60 -3.98 -6.78 -2.87 -2.13
Bud Neck 1.76 -0.45 1.45 -1.75 3.79 1.00 -3.64 -1.84 -3.68 2.69 1.77 -3.84 -0.38 -4.03 4.11
Bud Site 1.55 -0.41 0.56 -2.00 1.37 -1.44 -2.94 -5.03 -3.39 -1.84 -0.07 -3.01 -3.91 -4.00 0.34
Cell Periphery 3.01 1.90 -1.39 -1.61 -1.53 -1.23 -3.38 -3.48 -2.00 -3.16 -1.07 -3.09 -2.87 -2.43 -2.71
Cytoplasm 0.16 0.53 1.04 1.30 1.78 -2.11 -4.63 -2.88 -0.93 0.08 -1.75 -2.83 -1.78 0.38 0.71
Cytoplasmic Foci -0.37 -2.62 3.65 7.50 7.60 1.64 -3.52 4.26 2.64 4.77 0.25 -5.05 6.10 7.03 8.62
Eisosomes -0.69 2.62 -5.11 -5.44 -8.20 0.91 -2.25 -1.08 -1.22 -0.61 -0.81 2.98 -2.97 -2.80 -3.58
Endoplasmic Reticulum 0.88 0.01 0.43 -0.78 0.49 -0.99 -2.66 -3.26 -2.94 -2.49 -1.13 -2.53 -3.39 -3.01 -2.34
Endosome 1.87 -1.83 2.97 3.02 5.28 -2.13 -2.11 -3.18 -0.56 0.45 -1.04 -4.54 -0.30 0.82 4.48
Golgi -3.23 -3.18 -1.13 2.66 2.61 -3.94 -2.00 -5.26 -1.37 -1.30 -5.08 -3.96 -4.80 0.21 0.22
Lipid Particles -0.85 -2.66 1.74 2.81 5.04 3.20 -0.75 0.43 -2.09 0.99 1.32 -2.50 1.82 0.48 3.89
Mitochondria -1.03 2.88 -2.20 -2.07 -6.87 1.44 5.19 6.75 5.24 -2.65 1.56 8.18 4.29 3.00 -5.67
Mitotic Spindle -1.67 -3.65 -1.15 0.50 2.84 1.23 -2.30 -2.69 -3.36 1.69 -0.90 -4.26 -2.72 -1.82 3.01
None 1.18 -0.63 -1.02 -5.14 -0.92 3.14 -1.13 -6.18 -8.40 -2.67 3.08 -2.52 -5.80 -9.69 -2.94
Nuclear Periphery 1.08 -3.84 0.82 -1.60 4.38 1.68 -1.81 -2.23 -5.45 0.95 1.85 -4.12 -1.18 -5.62 3.82
Nuclear Periphery Foci 1.45 -1.13 1.65 2.78 3.95 2.41 -1.56 0.49 -4.01 1.94 2.66 -2.81 1.94 -2.55 3.95
Nucleolus 1.63 -0.67 1.88 0.88 2.74 0.49 -1.73 -1.69 -2.38 1.24 1.24 -2.48 0.46 -1.30 2.71
Nucleus 0.95 1.00 1.11 1.32 2.33 -1.01 -1.22 -1.97 0.84 -1.23 -0.09 0.64 0.97 1.22 1.54
Peroxisomes 2.15 2.31 2.92 1.76 1.12 0.63 -3.19 5.30 5.23 4.23 1.33 -1.39 4.94 4.80 4.76
Vacuole 1.99 -1.36 1.85 -0.42 4.86 -0.26 -2.30 -6.41 -6.41 -1.96 0.77 -4.94 -4.78 -6.10 1.33
Vacuole Periphery 0.85 -5.51 -2.15 -3.92 4.01 -0.95 -2.60 -11.23 -10.33 -3.37 -0.67 -8.19 -11.57 -10.95 -1.54
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mrp13

Mrp13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available