Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.06 0.08 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.97 0.96 0.89 0.87 0.75 0.68 0.74 0.73 0.97 0.98 0.99 0.99 0.99 0.99 0.95 0.95 0.89 0.9 0.87 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.11 0.2 0.25 0.15 0.18 0 0 0 0.07 0 0.07 0 0 0.06 0.06 0.08 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 2 9 12 8 11 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 0 2 2 8 16 19 38 23 24 0 2 4 1 1 0 0 0 0 0 0 0
Cytoplasm 274 105 159 266 264 245 372 238 322 186 194 201 370 392 310 308 328 243 99 143 213 339 306
Endoplasmic Reticulum 3 2 3 7 10 22 48 65 118 38 49 7 9 5 21 13 23 4 1 10 14 29 29
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 1 0 2 0 7 25 10 8 0 1 0 2 1 0 0 0 0 0 1 1
Mitochondria 0 0 0 0 0 1 1 1 0 5 0 0 0 0 1 1 4 0 0 0 0 1 0
Nucleus 1 0 0 0 1 2 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 1 4 0 1 0 1 2 0 0 0 2 0 2 2 0 1 2 2
Unique Cell Count 278 107 160 275 275 275 427 319 471 252 265 207 379 396 314 310 330 257 105 161 237 391 348
Labelled Cell Count 280 107 162 276 278 285 443 339 517 272 290 210 383 402 336 326 355 257 105 161 237 391 348


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 136.1 130.8 105.4 102.0 84.2 48.0 57.3 48.0 48.1 45.1 43.7 141.3 121.7 138.1 194.8 199.9 201.7 128.0 136.3 145.4
Std Deviation (1e-4) 26.3 22.8 22.0 25.7 19.1 16.6 13.6 12.2 11.1 12.8 12.1 28.4 27.8 25.0 43.1 48.6 42.5 26.8 32.0 28.9
Intensity Change (Log2) -0.05 -0.32 -1.13 -0.88 -1.14 -1.13 -1.23 -1.27 0.42 0.21 0.39 0.89 0.92 0.94 0.28 0.37 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3.2 3.7 3.9 3.9 0 0 0 0 0 0
Cytoplasm -1.8 -2.1 -4.0 -4.5 -6.8 -8.0 -6.9 -7.0 -1.6 -1.4 -0.4 -0.7 0 0
Endoplasmic Reticulum 0 0 2.6 3.6 5.5 6.4 4.4 5.1 0 0 0 2.3 1.3 2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 3.0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 114.2217 137.4384 121.8831 114.2751 92.7652 139.5291 139.9002 180.8319 162.3871 148.472 133.9828 157.3633 144.4183 174.8526 155.8812 148.3898 136.5612 157.5048
Actin 0.0029 0.0005 0.0026 0.0004 0.003 0.0014 0.0083 0.0003 0.0094 0.0333 0.0022 0.0028 0.0024 0.0005 0.0193 0.0014 0.0254 0.0024
Bud 0.0013 0.001 0.002 0.0024 0.0019 0.0017 0.0016 0.0006 0.0013 0.0018 0.0013 0.0022 0.0041 0.001 0.0028 0.0029 0.0065 0.006
Bud Neck 0.0008 0.001 0.0014 0.0006 0.0014 0.0016 0.0011 0.0006 0.0009 0.002 0.0017 0.0015 0.0019 0.0009 0.0017 0.0009 0.0016 0.0044
Bud Periphery 0.003 0.0009 0.0022 0.0068 0.0016 0.0019 0.0022 0.0005 0.0013 0.0014 0.0016 0.0013 0.0052 0.001 0.0024 0.0042 0.0042 0.0017
Bud Site 0.0007 0.0005 0.0013 0.0003 0.0047 0.0011 0.0011 0.0005 0.0029 0.0008 0.0005 0.0004 0.0018 0.0023 0.0022 0.0011 0.0032 0.0005
Cell Periphery 0.048 0.0251 0.0478 0.0228 0.0041 0.0623 0.0477 0.0264 0.025 0.042 0.0139 0.0311 0.0636 0.0198 0.0263 0.047 0.0072 0.0077
Cytoplasm 0.5489 0.8438 0.6206 0.5993 0.6095 0.3331 0.5529 0.8759 0.7379 0.52 0.5966 0.7286 0.5573 0.889 0.6915 0.5724 0.6907 0.7662
Cytoplasmic Foci 0.0073 0.0021 0.0102 0.0077 0.0966 0.0062 0.0177 0.0011 0.008 0.0039 0.0312 0.0046 0.0042 0.001 0.0083 0.0251 0.0243 0.0067
Eisosomes 0.0006 0 0.0004 0.0001 0.0002 0.0009 0.001 0 0.0004 0.0006 0.0011 0 0.0007 0 0.0001 0.0004 0.0003 0.0002
Endoplasmic Reticulum 0.125 0.0407 0.0557 0.0032 0.0058 0.2204 0.0614 0.0178 0.0153 0.003 0.0039 0.0106 0.0601 0.0221 0.0142 0.0022 0.0067 0.0462
Endosome 0.0142 0.0056 0.0148 0.0129 0.0316 0.0341 0.011 0.0025 0.0084 0.0091 0.0088 0.013 0.0125 0.0017 0.0074 0.0043 0.027 0.017
Golgi 0.0029 0.0013 0.0029 0.0007 0.0079 0.0285 0.0031 0.0006 0.0038 0.0008 0.0013 0.0052 0.0028 0.0004 0.0024 0.0005 0.009 0.0253
Lipid Particles 0.0033 0.0014 0.0064 0.0051 0.0217 0.0057 0.0084 0.002 0.0049 0.005 0.0145 0.0015 0.0045 0.0014 0.0017 0.0093 0.0059 0.0017
Mitochondria 0.0154 0.0012 0.0089 0.0245 0.0093 0.0192 0.0098 0.001 0.0086 0.0072 0.0157 0.0187 0.007 0.0004 0.0064 0.0209 0.0145 0.0108
None 0.0024 0.0003 0.0076 0.002 0.0113 0.0013 0.0089 0.0003 0.0032 0.0124 0.0249 0.0007 0.0047 0.0002 0.0031 0.0066 0.0112 0.0011
Nuclear Periphery 0.0434 0.0044 0.0117 0.0054 0.0053 0.0835 0.0428 0.0043 0.0078 0.0112 0.0083 0.0056 0.0453 0.0042 0.0112 0.0066 0.0314 0.0074
Nucleolus 0.005 0.0002 0.0065 0.005 0.0397 0.0128 0.0194 0.0003 0.005 0.0181 0.0388 0.0441 0.0032 0.0002 0.005 0.0066 0.0047 0.0006
Nucleus 0.0076 0.0019 0.0056 0.0045 0.0092 0.0052 0.0134 0.0017 0.0047 0.0131 0.0098 0.0183 0.0204 0.003 0.0103 0.0082 0.0132 0.0054
Peroxisomes 0.0006 0 0.0007 0.0012 0.0089 0.0004 0.0035 0 0.0097 0.0004 0.0093 0.0002 0.0003 0 0.0016 0.0008 0.0013 0.0003
Punctate Nuclear 0.0016 0 0.0013 0.0003 0.0104 0.0001 0.0033 0 0.0005 0.0003 0.0112 0.0001 0.0061 0 0.0004 0.0042 0.0021 0.0001
Vacuole 0.1287 0.0601 0.157 0.2229 0.0825 0.1296 0.1513 0.0591 0.1204 0.2535 0.1512 0.0906 0.16 0.0469 0.1436 0.2174 0.0732 0.0656
Vacuole Periphery 0.0361 0.008 0.0322 0.0719 0.0333 0.049 0.0303 0.0044 0.0206 0.0602 0.0523 0.0188 0.0319 0.0039 0.0381 0.0568 0.0364 0.0228

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1653.0049 1856.266 2241.8083 1990.3647 2046.4565 1726.6597 1665.531 1598.8356 1725.0423 1673.2775
Translational Efficiency 1.474 1.4215 1.2611 1.389 1.4265 1.4099 1.4338 1.5787 1.359 1.5189

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1906 899 1518 102 1858 2363 2387 1215 3764 3262 3905 1317

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6206.05 7113.24 10439.21 9280.09 6814.20 7728.28 10565.42 9439.12 6506.25 7558.78 10516.36 9426.80
Standard Deviation 1503.40 1882.57 1987.06 2248.10 1404.22 1614.49 2000.24 2637.03 1486.71 1714.78 1996.08 2609.33
Intensity Change Log 2 0.196832 0.750265 0.580463 0.181603 0.632734 0.470108 0.188882 0.689950 0.523762

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000120 0.000137 0.000145 0.000044 0.000059 0.000076 0.000924 0.000038 0.000076 0.000100 0.000863
Bud Neck 0.000125 0.000752 0.001505 0.003421 0.000215 0.000309 0.000415 0.001284 0.000170 0.000431 0.000839 0.001450
Bud Site 0.001278 0.004631 0.002988 0.005874 0.001237 0.003814 0.002590 0.011673 0.001258 0.004039 0.002745 0.011224
Cell Periphery 0.021614 0.036718 0.052461 0.077499 0.032780 0.014656 0.044253 0.074452 0.027126 0.020736 0.047444 0.074688
Cytoplasm 0.589332* 0.517718* 0.334589* 0.146061* 0.405050* 0.613464* 0.578570* 0.464868* 0.498366* 0.587076* 0.483727* 0.440177*
Cytoplasmic Foci 0.010527 0.010030 0.005958 0.006904 0.013642 0.015852 0.003021 0.005195 0.012064 0.014248 0.004163 0.005327
Eisosomes 0.000001 0.000003 0.000006 0.000007 0.000002 0.000001 0.000002 0.000007 0.000002 0.000002 0.000004 0.000007
Endoplasmic Reticulum 0.003466 0.001890 0.002168 0.000531 0.006319 0.003128 0.001641 0.001730 0.004875 0.002787 0.001846 0.001637
Endosome 0.000752 0.001012 0.000745 0.000951 0.000948 0.001212 0.000425 0.000953 0.000849 0.001157 0.000550 0.000952
Golgi 0.055312 0.074385 0.050945 0.046710 0.118172* 0.063365 0.020803 0.027270 0.086341 0.066402 0.032520 0.028776
Lipid Particles 0.016526 0.026181 0.026380 0.055371 0.023137 0.013297 0.024628 0.039410 0.019789 0.016848 0.025309 0.040646
Mitochondria 0.000242 0.000244 0.000265 0.000063 0.000288 0.000495 0.000206 0.000599 0.000265 0.000425 0.000229 0.000558
Mitotic Spindle 0.000521 0.000746 0.000923 0.000967 0.000844 0.000940 0.000339 0.001333 0.000680 0.000886 0.000566 0.001304
None 0.002412 0.002939 0.001135 0.000381 0.001551 0.001426 0.001008 0.002058 0.001987 0.001843 0.001058 0.001928
Nuclear Periphery 0.000676 0.001289 0.003034 0.001256 0.001560 0.000465 0.000711 0.000706 0.001112 0.000692 0.001614 0.000748
Nuclear Periphery Foci 0.010692 0.016885 0.015743 0.009535 0.015714 0.008669 0.007203 0.006628 0.013171 0.010933 0.010523 0.006853
Nucleolus 0.000092 0.000552 0.000126 0.000123 0.000079 0.000078 0.000099 0.000233 0.000086 0.000208 0.000110 0.000225
Nucleus 0.002570 0.009188 0.008749 0.009581 0.002183 0.002686 0.004514 0.007958 0.002379 0.004478 0.006160 0.008083
Peroxisomes 0.000076 0.000046 0.000029 0.000076 0.000048 0.000067 0.000107 0.000149 0.000063 0.000061 0.000077 0.000143
Vacuole 0.126713 0.181357 0.342309* 0.560291* 0.136900 0.118306 0.249005* 0.293351* 0.131741 0.135683 0.285275* 0.314025*
Vacuole Periphery 0.157040* 0.113312* 0.149803* 0.074254 0.239289* 0.137711* 0.060384 0.059220 0.197640* 0.130987* 0.095144 0.060384

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.03 -8.37 -5.49 -0.91 -0.13 -1.87 -4.81 -1.49 -1.46 -1.43 -5.24 -10.03 -1.53 -1.44 -1.38
Bud Neck -6.66 -8.52 -3.37 -2.92 -2.22 -2.90 -5.84 -8.14 -7.19 -6.32 -7.29 -10.06 -8.61 -6.87 -3.80
Bud Site -3.99 -6.18 -3.57 -0.53 -1.96 -7.25 -4.89 -8.33 -6.17 -7.15 -8.00 -7.70 -8.62 -5.94 -7.17
Cell Periphery -6.18 -13.03 -5.34 -3.38 -1.63 11.56 -5.17 -10.99 -17.31 -7.68 5.37 -12.86 -13.91 -16.05 -7.32
Cytoplasm 5.10 22.05 15.39 11.81 5.49 -19.07 -15.53 -4.89 11.22 8.26 -10.15 2.20 4.32 11.60 2.75
Cytoplasmic Foci 0.23 4.48 1.57 1.24 -1.07 -1.69 10.42 6.54 9.57 -4.93 -2.44 11.55 6.96 9.24 -3.14
Eisosomes -6.54 -9.83 -5.63 -3.82 -1.07 2.62 -1.53 -5.19 -5.65 -4.92 -3.03 -9.83 -6.10 -5.64 -3.56
Endoplasmic Reticulum 3.88 3.71 9.98 4.84 6.37 4.51 7.30 6.61 2.93 -0.02 4.85 8.33 7.08 2.90 0.72
Endosome -3.00 -0.06 -0.52 0.69 -0.50 -3.86 10.78 0.13 2.27 -4.29 -5.85 8.66 -0.70 2.04 -3.38
Golgi -4.89 2.19 1.80 3.67 1.21 15.35 31.26 28.15 16.02 -3.36 8.13 26.67 25.55 18.21 3.31
Lipid Particles -4.65 -7.12 -4.23 -2.94 -2.94 7.22 -0.90 -6.30 -11.16 -5.89 2.77 -5.32 -8.92 -10.26 -6.37
Mitochondria -0.44 -1.03 5.17 4.51 4.84 -3.80 2.55 -1.56 -0.41 -1.99 -4.22 1.24 -1.62 -0.62 -1.78
Mitotic Spindle -3.91 -6.51 -1.49 -0.69 0.18 -1.71 8.79 -0.94 -0.72 -1.73 -4.12 1.85 -1.24 -0.83 -1.38
None -0.91 3.01 4.60 6.22 6.03 0.72 1.68 -1.20 -1.39 -1.96 0.53 3.20 0.03 -0.28 -1.96
Nuclear Periphery -4.36 -10.89 -4.06 0.50 7.23 6.73 5.00 5.21 -5.93 0.45 4.25 -4.59 4.11 -0.62 9.37
Nuclear Periphery Foci -5.31 -6.47 1.02 4.83 5.01 9.81 12.46 12.85 4.03 1.48 3.78 4.90 12.00 7.69 7.95
Nucleolus -1.30 -6.48 -2.33 1.16 -0.75 -0.22 -2.44 -2.30 -2.28 -2.01 -1.28 -5.24 -2.30 0.08 -1.87
Nucleus -5.86 -8.43 -3.47 -2.05 -1.89 -2.37 -6.64 -8.73 -7.71 -4.92 -5.98 -10.66 -9.29 -6.34 -3.71
Peroxisomes 2.07 4.01 0.09 -0.64 -1.11 -1.19 -3.26 -4.03 -3.63 -1.67 0.16 -1.06 -3.69 -3.98 -3.21
Vacuole -6.95 -28.69 -14.61 -12.31 -6.71 3.28 -18.18 -18.31 -20.49 -4.41 -1.49 -31.96 -22.32 -21.01 -2.61
Vacuole Periphery 7.38 2.12 10.95 5.57 9.70 17.17* 34.65* 33.79* 19.28 1.33 15.85 27.83 35.79 20.35 12.19
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl11b

Rpl11b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl11b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available