Standard name | |
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Human Ortholog | |
Description | Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 16 | 12 | – | 10 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | – | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 7 | 1 | 12 | 14 | 31 | 25 | 16 | – | 36 | 42 | 2 | 2 | 2 | 3 | 8 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 120 | 436 | 368 | 244 | 255 | 221 | 339 | 315 | 232 | – | 210 | 235 | 262 | 356 | 355 | 241 | 264 | 256 | 131 | 425 | 362 | 191 | 211 | 321 |
Endoplasmic Reticulum | 0 | 4 | 7 | 2 | 8 | 11 | 63 | 60 | 39 | – | 31 | 65 | 2 | 2 | 6 | 9 | 17 | 14 | 1 | 1 | 6 | 2 | 7 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 6 | 19 | – | 13 | 14 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 1 | 0 | 2 | 0 | 2 | 2 | 0 | 0 | 4 | 4 | 3 | 0 | 0 |
Unique Cell Count | 120 | 444 | 373 | 247 | 268 | 239 | 415 | 401 | 298 | 273 | 332 | 264 | 358 | 361 | 248 | 270 | 258 | 135 | 442 | 380 | 203 | 224 | 339 | |
Labelled Cell Count | 120 | 447 | 384 | 248 | 278 | 247 | 447 | 429 | 322 | 306 | 381 | 266 | 363 | 366 | 259 | 292 | 277 | 135 | 442 | 380 | 203 | 224 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 107.7 | 104.0 | 66.2 | 63.5 | 55.5 | 48.3 | 44.4 | 40.8 | 36.9 | – | 34.4 | 33.3 | 87.9 | 82.7 | 68.9 | 115.2 | 121.7 | 124.7 | 89.6 | 89.4 | 96.1 |
Std Deviation (1e-4) | 24.1 | 21.3 | 13.5 | 12.5 | 12.0 | 10.7 | 9.0 | 9.3 | 8.7 | – | 7.8 | 7.9 | 15.8 | 18.3 | 15.9 | 31.1 | 38.5 | 39.5 | 23.7 | 25.4 | 23.2 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.25 | -0.45 | -0.58 | -0.7 | -0.84 | – | -0.94 | -0.99 | 0.41 | 0.32 | 0.06 | 0.8 | 0.88 | 0.91 | 0.44 | 0.43 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.9 | 3.9 | – | 0 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.9 | 2.6 | 3.7 | 3.0 | 2.5 | – | 5.7 | 5.6 | 0 | 0 | 0 | 0 | 0.9 | 0 |
Cytoplasm | 0.1 | -2.7 | -3.9 | -7.8 | -8.7 | -8.7 | – | -8.9 | -10.5 | 0.7 | 1.1 | -0.4 | -1.3 | -0.9 | 0.7 |
Endoplasmic Reticulum | 0 | 0.9 | 1.9 | 6.6 | 6.5 | 5.7 | – | 5.1 | 7.7 | 0 | 0 | -0.2 | 1.3 | 2.9 | 2.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.9 | – | 4.3 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 65.735 | 110.5874 | 99.6922 | 94.3489 | 96.2217 | 99.5278 | 74.6424 | 120.4267 | 109.0319 | 104.829 | 70.54 | 107.1728 | 90.4277 | 116.6255 | 104.0802 | 105.3266 | 93.3884 | 115.525 |
Actin | 0.0317 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0015 | 0.0244 | 0.0004 | 0.0005 | 0.0009 | 0.0023 | 0.0024 | 0.0026 | 0.0003 | 0.0138 | 0.0367 | 0.0054 | 0.0003 |
Bud | 0.0021 | 0.0006 | 0.0016 | 0.0019 | 0.0013 | 0.001 | 0.0015 | 0.0002 | 0.0014 | 0.0013 | 0.0007 | 0.0004 | 0.0009 | 0.0007 | 0.0009 | 0.0006 | 0.0011 | 0.0002 |
Bud Neck | 0.0029 | 0.001 | 0.0016 | 0.0016 | 0.0015 | 0.0091 | 0.0014 | 0.0004 | 0.0009 | 0.0012 | 0.0006 | 0.0013 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0008 | 0.0019 |
Bud Periphery | 0.003 | 0.0005 | 0.0022 | 0.0022 | 0.0021 | 0.0008 | 0.0017 | 0.0002 | 0.0015 | 0.0006 | 0.0005 | 0.0004 | 0.001 | 0.001 | 0.0008 | 0.0004 | 0.0009 | 0.0002 |
Bud Site | 0.0034 | 0.0011 | 0.0009 | 0.0004 | 0.0004 | 0.0002 | 0.0037 | 0.0003 | 0.0028 | 0.0005 | 0.0008 | 0.0004 | 0.0007 | 0.0009 | 0.0006 | 0.0009 | 0.0004 | 0.0001 |
Cell Periphery | 0.0524 | 0.0147 | 0.051 | 0.0469 | 0.0416 | 0.0109 | 0.0051 | 0.0109 | 0.0188 | 0.0072 | 0.0034 | 0.0096 | 0.0106 | 0.0052 | 0.0022 | 0.012 | 0.001 | 0.0023 |
Cytoplasm | 0.5029 | 0.9003 | 0.734 | 0.5659 | 0.6657 | 0.7942 | 0.7448 | 0.9515 | 0.8953 | 0.797 | 0.6877 | 0.8918 | 0.8005 | 0.9652 | 0.8785 | 0.8788 | 0.8112 | 0.9481 |
Cytoplasmic Foci | 0.0103 | 0.0003 | 0.0015 | 0.0007 | 0.001 | 0.0021 | 0.0174 | 0.0005 | 0.0016 | 0.0183 | 0.0349 | 0.0019 | 0.0135 | 0.0004 | 0.0094 | 0.0014 | 0.0201 | 0.0017 |
Eisosomes | 0.0016 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0 | 0.0004 | 0.0005 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0578 | 0.0054 | 0.0136 | 0.0228 | 0.0012 | 0.0648 | 0.0148 | 0.0044 | 0.0062 | 0.0018 | 0.0095 | 0.02 | 0.0233 | 0.0057 | 0.0052 | 0.0009 | 0.0053 | 0.0197 |
Endosome | 0.0146 | 0.0006 | 0.0017 | 0.0033 | 0.0016 | 0.0063 | 0.0113 | 0.0004 | 0.0016 | 0.0022 | 0.0294 | 0.0044 | 0.0044 | 0.0004 | 0.0068 | 0.0017 | 0.0121 | 0.004 |
Golgi | 0.0033 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0044 | 0.0001 | 0.0002 | 0.0003 | 0.0037 | 0.0006 | 0.0008 | 0.0001 | 0.0019 | 0.0006 | 0.0082 | 0.0003 |
Lipid Particles | 0.0101 | 0.0004 | 0.0021 | 0.0014 | 0.0004 | 0.0006 | 0.0089 | 0.0006 | 0.0011 | 0.0077 | 0.036 | 0.0013 | 0.0048 | 0.0003 | 0.0065 | 0.0013 | 0.0161 | 0.0002 |
Mitochondria | 0.0325 | 0.0005 | 0.0073 | 0.0005 | 0.0005 | 0.0009 | 0.0192 | 0.0006 | 0.0009 | 0.004 | 0.031 | 0.0009 | 0.0066 | 0.0002 | 0.0062 | 0.0005 | 0.0197 | 0.0002 |
None | 0.0088 | 0.0003 | 0.0013 | 0.0003 | 0.0006 | 0.0004 | 0.0083 | 0.0003 | 0.0012 | 0.0153 | 0.0156 | 0.0015 | 0.0111 | 0.0002 | 0.0071 | 0.0002 | 0.0153 | 0.0002 |
Nuclear Periphery | 0.0495 | 0.0068 | 0.0213 | 0.0305 | 0.0039 | 0.0173 | 0.0219 | 0.0038 | 0.0036 | 0.0042 | 0.0341 | 0.0123 | 0.0137 | 0.0019 | 0.0059 | 0.0031 | 0.007 | 0.0032 |
Nucleolus | 0.0243 | 0.0009 | 0.0069 | 0.0009 | 0.0006 | 0.0002 | 0.0044 | 0.0015 | 0.0008 | 0.0066 | 0.0172 | 0.0015 | 0.0091 | 0.0001 | 0.0017 | 0.0003 | 0.0037 | 0 |
Nucleus | 0.0314 | 0.0059 | 0.0112 | 0.016 | 0.0055 | 0.0068 | 0.0124 | 0.0031 | 0.0034 | 0.0056 | 0.0107 | 0.0113 | 0.0123 | 0.0017 | 0.0051 | 0.0025 | 0.0041 | 0.0017 |
Peroxisomes | 0.0021 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0038 | 0 | 0.0001 | 0.0005 | 0.0056 | 0.0002 | 0.0022 | 0 | 0.002 | 0.0003 | 0.009 | 0 |
Punctate Nuclear | 0.0086 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0123 | 0.0004 | 0.0001 | 0.0009 | 0.0032 | 0.0051 | 0.0039 | 0 | 0.003 | 0.0001 | 0.0008 | 0 |
Vacuole | 0.121 | 0.0559 | 0.1219 | 0.2356 | 0.2501 | 0.0696 | 0.0672 | 0.0193 | 0.0545 | 0.1104 | 0.053 | 0.0281 | 0.0618 | 0.0147 | 0.0326 | 0.0516 | 0.0424 | 0.0136 |
Vacuole Periphery | 0.0258 | 0.0043 | 0.0189 | 0.0686 | 0.0218 | 0.0125 | 0.0105 | 0.0011 | 0.0034 | 0.0131 | 0.0197 | 0.0046 | 0.0155 | 0.0007 | 0.0089 | 0.0057 | 0.0152 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 411.5715 | 447.5143 | 472.721 | 421.8083 | 408.6847 | 407.8507 | 420.967 | 371.4886 | 385.8672 | 372.3785 |
Translational Efficiency | 1.1444 | 1.1484 | 1.0082 | 1.2251 | 1.0143 | 0.987 | 0.9891 | 0.9115 | 0.8553 | 0.8464 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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