Standard name | |
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Human Ortholog | |
Description | Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 6 | 12 | 6 | 5 | 3 | 2 | 5 | 0 | 4 | 2 | 1 | 2 | 0 | 28 | 21 | 29 | 6 | 3 | 16 | 7 | 20 | 24 |
Bud | 0 | 1 | 17 | 13 | 17 | 29 | 17 | 31 | 25 | 21 | 26 | 35 | 2 | 3 | 8 | 3 | 2 | 5 | 2 | 1 | 4 | 3 | 6 | 6 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 5 | 1 | 7 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 4 | 10 | 2 | 10 | 5 | 6 | 10 | 8 | 7 | 0 | 0 | 0 | 12 | 6 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 47 | 193 | 168 | 215 | 196 | 314 | 342 | 171 | 164 | 168 | 196 | 36 | 70 | 114 | 177 | 121 | 184 | 40 | 57 | 92 | 12 | 36 | 30 |
Endoplasmic Reticulum | 5 | 2 | 10 | 7 | 6 | 11 | 4 | 9 | 6 | 6 | 10 | 11 | 1 | 2 | 2 | 19 | 9 | 17 | 7 | 1 | 15 | 3 | 11 | 16 |
Endosome | 5 | 0 | 14 | 7 | 35 | 14 | 32 | 37 | 3 | 3 | 0 | 1 | 1 | 2 | 2 | 61 | 43 | 66 | 17 | 6 | 36 | 5 | 12 | 14 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 9 | 12 | 14 | 9 | 0 | 6 | 1 | 4 | 8 |
Mitochondria | 1 | 12 | 41 | 95 | 62 | 132 | 185 | 298 | 188 | 205 | 214 | 334 | 2 | 2 | 28 | 16 | 9 | 13 | 5 | 6 | 108 | 8 | 11 | 15 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 2 | 3 | 1 | 0 | 1 | 2 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 3 | 1 | 5 | 2 | 6 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 |
SpindlePole | 1 | 0 | 5 | 7 | 14 | 10 | 14 | 17 | 3 | 1 | 6 | 14 | 0 | 0 | 0 | 17 | 22 | 32 | 1 | 1 | 8 | 0 | 3 | 1 |
Vac/Vac Membrane | 4 | 1 | 4 | 1 | 3 | 6 | 9 | 41 | 3 | 5 | 5 | 14 | 0 | 1 | 0 | 26 | 18 | 26 | 4 | 8 | 21 | 2 | 8 | 2 |
Unique Cell Count | 39 | 55 | 263 | 264 | 315 | 328 | 483 | 620 | 320 | 331 | 342 | 483 | 42 | 80 | 129 | 316 | 229 | 342 | 99 | 92 | 326 | 50 | 135 | 130 |
Labelled Cell Count | 46 | 64 | 298 | 315 | 374 | 408 | 604 | 796 | 415 | 421 | 449 | 625 | 45 | 85 | 156 | 372 | 268 | 404 | 99 | 92 | 326 | 50 | 135 | 130 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 4.8 | 5.1 | 4.9 | 5.2 | 4.7 | 5.0 | 4.5 | 4.3 | 4.4 | 4.3 | 4.2 | 6.5 | 7.2 | 6.5 | 8.5 | 9.2 | 9.5 | 5.9 | 7.2 | 8.0 |
Std Deviation (1e-4) | 1.0 | 0.7 | 0.9 | 1.6 | 1.2 | 1.2 | 1.6 | 1.1 | 1.0 | 1.4 | 1.0 | 1.1 | 1.1 | 1.0 | 1.0 | 1.2 | 1.4 | 1.6 | 1.5 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.03 | -0.11 | -0.03 | -0.17 | -0.23 | -0.19 | -0.24 | -0.26 | 0.36 | 0.5 | 0.36 | 0.74 | 0.86 | 0.9 | 0.21 | 0.52 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.6 | 3.4 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7623 | 3.4122 | 3.1103 | 2.5279 | 2.7073 | 3.0593 | 2.042 | 3.0316 | 2.7337 | 2.6234 | 2.3402 | 3.0537 | 3.8319 | 4.7483 | 4.1961 | 3.8676 | 4.1569 | 4.1512 |
Actin | 0.0231 | 0.0093 | 0.0209 | 0.0023 | 0.0102 | 0.0121 | 0.0194 | 0.0069 | 0.0166 | 0.0115 | 0.0018 | 0.0092 | 0.0043 | 0.0168 | 0.0145 | 0.0028 | 0.0086 | 0.0095 |
Bud | 0.0032 | 0.0028 | 0.002 | 0.0176 | 0.0018 | 0.0019 | 0.0011 | 0.0005 | 0.0017 | 0.0009 | 0.0024 | 0.0003 | 0.0016 | 0.0028 | 0.0021 | 0.0006 | 0.0014 | 0.004 |
Bud Neck | 0.0065 | 0.0016 | 0.0033 | 0.0018 | 0.0016 | 0.0194 | 0.0057 | 0.0015 | 0.0009 | 0.0006 | 0.0015 | 0.0139 | 0.0013 | 0.0007 | 0.0048 | 0.0005 | 0.0007 | 0.004 |
Bud Periphery | 0.0056 | 0.0018 | 0.002 | 0.0101 | 0.0014 | 0.0031 | 0.0014 | 0.0004 | 0.0015 | 0.0012 | 0.0023 | 0.0004 | 0.0015 | 0.001 | 0.001 | 0.0004 | 0.0018 | 0.0042 |
Bud Site | 0.0294 | 0.0359 | 0.0065 | 0.0041 | 0.0021 | 0.0027 | 0.0081 | 0.0059 | 0.0118 | 0.0021 | 0.0097 | 0.0003 | 0.0502 | 0.0391 | 0.0099 | 0.0007 | 0.004 | 0.0005 |
Cell Periphery | 0.0035 | 0.0015 | 0.0014 | 0.0033 | 0.0004 | 0.0008 | 0.0009 | 0.0006 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0007 | 0.0008 | 0.0005 | 0.0003 | 0.0005 | 0.0004 |
Cytoplasm | 0.5259 | 0.6934 | 0.6725 | 0.6734 | 0.5129 | 0.6471 | 0.5649 | 0.7522 | 0.6304 | 0.5674 | 0.7053 | 0.6892 | 0.6074 | 0.6676 | 0.6196 | 0.6449 | 0.6186 | 0.6451 |
Cytoplasmic Foci | 0.0602 | 0.0469 | 0.0633 | 0.0237 | 0.0956 | 0.0704 | 0.081 | 0.0691 | 0.0789 | 0.0842 | 0.0551 | 0.0648 | 0.0691 | 0.0508 | 0.1001 | 0.0894 | 0.0656 | 0.0739 |
Eisosomes | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0371 | 0.0146 | 0.0094 | 0.0135 | 0.0176 | 0.0178 | 0.0167 | 0.0041 | 0.0047 | 0.0038 | 0.003 | 0.0074 | 0.0106 | 0.0042 | 0.0101 | 0.0321 | 0.0063 | 0.0102 |
Endosome | 0.132 | 0.1121 | 0.1202 | 0.1215 | 0.1962 | 0.1293 | 0.1209 | 0.058 | 0.0991 | 0.1435 | 0.0999 | 0.0793 | 0.1574 | 0.0723 | 0.1505 | 0.123 | 0.1075 | 0.123 |
Golgi | 0.038 | 0.0305 | 0.0224 | 0.0059 | 0.0443 | 0.0329 | 0.0335 | 0.0116 | 0.0184 | 0.0182 | 0.0286 | 0.0235 | 0.035 | 0.0131 | 0.0221 | 0.0173 | 0.0264 | 0.0396 |
Lipid Particles | 0.0097 | 0.001 | 0.0061 | 0.0007 | 0.0168 | 0.0039 | 0.0093 | 0.0018 | 0.0081 | 0.0048 | 0.0047 | 0.0055 | 0.0013 | 0.0005 | 0.0015 | 0.0008 | 0.0205 | 0.0006 |
Mitochondria | 0.0499 | 0.008 | 0.0266 | 0.0143 | 0.0352 | 0.0298 | 0.0102 | 0.0017 | 0.0159 | 0.0106 | 0.0053 | 0.0066 | 0.0046 | 0.0028 | 0.0062 | 0.0084 | 0.0065 | 0.0178 |
None | 0.0261 | 0.0218 | 0.0199 | 0.0733 | 0.0349 | 0.0105 | 0.0929 | 0.0742 | 0.0935 | 0.1131 | 0.0642 | 0.0876 | 0.0422 | 0.1204 | 0.0378 | 0.0674 | 0.1082 | 0.0568 |
Nuclear Periphery | 0.0055 | 0.0015 | 0.002 | 0.0015 | 0.0015 | 0.0016 | 0.0085 | 0.0006 | 0.0008 | 0.0017 | 0.0007 | 0.0005 | 0.0016 | 0.0006 | 0.0037 | 0.0012 | 0.0038 | 0.0006 |
Nucleolus | 0.002 | 0.0003 | 0.0004 | 0.001 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0012 | 0.0001 |
Nucleus | 0.0031 | 0.0015 | 0.0014 | 0.0086 | 0.0013 | 0.0012 | 0.0047 | 0.0009 | 0.0009 | 0.0012 | 0.0017 | 0.0005 | 0.0014 | 0.0009 | 0.0013 | 0.0008 | 0.0017 | 0.0006 |
Peroxisomes | 0.0072 | 0.0006 | 0.0023 | 0.0004 | 0.0101 | 0.0029 | 0.0069 | 0.0028 | 0.0047 | 0.009 | 0.0027 | 0.0062 | 0.0012 | 0.0007 | 0.004 | 0.0011 | 0.0061 | 0.0026 |
Punctate Nuclear | 0.0046 | 0.0009 | 0.0019 | 0.0004 | 0.0016 | 0.0008 | 0.0041 | 0.0012 | 0.001 | 0.0011 | 0.0008 | 0.0006 | 0.0012 | 0.0004 | 0.0014 | 0.0003 | 0.0046 | 0.0002 |
Vacuole | 0.0212 | 0.0115 | 0.0124 | 0.0166 | 0.01 | 0.008 | 0.0075 | 0.0044 | 0.0083 | 0.0175 | 0.0078 | 0.0026 | 0.0053 | 0.0035 | 0.0058 | 0.0058 | 0.0044 | 0.0047 |
Vacuole Periphery | 0.0057 | 0.0025 | 0.0031 | 0.0057 | 0.004 | 0.0033 | 0.0017 | 0.0007 | 0.002 | 0.0071 | 0.0018 | 0.0012 | 0.0018 | 0.0008 | 0.0021 | 0.0019 | 0.0014 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.0224 | 7.6061 | 12.3143 | 19.1278 | 14.0404 | 12.7558 | 17.2163 | 19.9366 | 22.0451 | 20.6851 |
Translational Efficiency | 0.6029 | 0.7542 | 0.7376 | 0.584 | 0.5098 | 0.6295 | 0.538 | 0.3637 | 0.5311 | 0.4709 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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