Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.05 0.07 0.09 0.07 0 0 0 0 0 0 0.09 0.14 0.12 0.1 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.1 0 0 0.28 0.14 0.14 0.11 0.65 0.32 0.46 0.4 0 0 0 0.09 0 0 0 0 0 0 0 0
Nucleus 0.86 0.82 0.85 0.83 0.74 0.8 0.81 0.84 0.67 0.83 0.7 0.75 0.84 0.83 0.78 0.82 0.82 0.81 0.61 0.7 0.69 0.63 0.59 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.07 0 0.05 0.07 0 0.07 0 0.1 0.06 0.1 0.09 0 0 0 0.09 0.08 0.05 0.13 0.08 0.11 0.2 0.2 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.05 0 0.05
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.08 0.05 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 1 0 0 0 2 0 0 1 0 0 0 1 0 0 3 2 1 1 1 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 3 0 3 1 8 3 4 7 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 25 9 9 11 12 9 10 8 5 10 8 29 48 36 35 23 24 1 9 2 1 4 6
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 6 6 13 0 0 0 0 0 0
Endosome 0 5 8 2 0 4 7 1 1 0 0 0 11 3 12 4 3 4 2 8 5 2 9 7
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 2 5 1 0 2 2
Mitochondria 5 33 8 3 33 25 30 26 153 46 116 98 0 1 1 34 10 11 1 8 3 1 3 4
Nucleus 48 262 166 104 87 145 171 192 159 118 176 183 270 291 226 302 236 217 54 253 156 114 206 200
Nuclear Periphery 0 0 0 1 1 2 3 1 0 2 2 4 0 0 0 2 9 3 0 1 1 1 1 2
Nucleolus 11 22 2 6 8 8 14 7 23 9 24 22 10 11 11 34 23 13 11 30 25 35 68 59
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1 0 0 0 0
SpindlePole 1 6 11 0 2 7 11 12 1 2 2 7 10 9 4 6 7 16 5 12 6 8 13 16
Vac/Vac Membrane 3 8 6 4 1 5 8 10 2 2 3 1 10 7 13 10 14 6 3 25 17 9 27 23
Unique Cell Count 56 321 196 125 118 181 211 229 237 143 251 245 323 350 290 367 287 267 89 364 227 181 351 344
Labelled Cell Count 70 362 210 129 147 209 256 260 357 187 338 333 341 371 306 435 333 307 89 364 227 181 351 344


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.1 6.0 5.6 4.5 5.0 5.0 5.3 4.0 4.4 4.4 4.5 6.4 6.6 6.2 5.5 7.2 6.7 6.3 6.8 6.8
Std Deviation (1e-4) 1.1 1.3 1.4 1.7 2.0 1.4 1.0 1.4 0.8 1.2 1.2 0.8 1.2 1.5 1.4 1.3 1.8 1.8 1.5 1.4 1.8
Intensity Change (Log2) – – – -0.08 -0.4 -0.26 -0.27 -0.19 -0.58 -0.43 -0.43 -0.41 0.1 0.13 0.04 -0.11 0.27 0.17 0.08 0.2 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.7 0.9 -0.2 -0.1 -0.6 -0.5 -0.3 -0.7 1.9 3.3 2.9 2.1 1.5 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Endosome 0 0 -1.0 -0.4 0 0 0 0 0 -0.4 0 0 0 0 -1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 3.5 2.8 0 0 0 0 0 0 0 2.2 -0.3 0
Nucleus -0.4 -2.4 -1.2 -1.0 -0.2 -4.2 -0.5 -3.6 -2.6 -0.3 -0.5 -1.9 -0.7 -0.7 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.9 0 0 0 0 0 0 0 1.9 3.8 3.4 2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 -0.8 -0.2 -0.2 0 0 0 0 -1.4 -1.8 -2.6 -2.6 -1.8 0.2
Vacuole 0 0 -0.2 0.4 0.7 0 0 0 0 0 0 0.8 -0.2 1.0 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.7358 0.0005 0.0034 0.0001 0.0416 0.012 0.0321 0.0007 0.007 0.0002 0.0008 0.0046 – – – – – –
Bud 0.0026 0.0027 0.0002 0 0.0005 0.0023 0.0021 0.0016 0.0021 0.0001 0 0.0017 – – – – – –
Bud Neck 0.0023 0 0.0005 0 0.0014 0.0016 0.0359 0.0001 0.0013 0 0 0.0056 – – – – – –
Bud Periphery 0.0022 0.0056 0.0002 0 0.0007 0.0028 0.0054 0.0015 0.0044 0.0001 0 0.0034 – – – – – –
Bud Site 0.0126 0.0008 0.0015 0 0.0014 0.0004 0.0663 0.0036 0.0118 0.0001 0.0001 0.0003 – – – – – –
Cell Periphery 0.0005 0.0001 0.0001 0 0.0002 0.0001 0.0076 0.0001 0.0002 0 0 0.0003 – – – – – –
Cytoplasm 0.0076 0.0224 0.0183 0.0306 0.0097 0.0555 0.0154 0.0167 0.0367 0.0153 0.0448 0.0321 – – – – – –
Cytoplasmic Foci 0.0638 0.0029 0.0097 0.0015 0.0308 0.0037 0.1089 0.0017 0.0068 0.0022 0.0206 0.0031 – – – – – –
Eisosomes 0.0009 0.0001 0.0001 0.0001 0.0003 0.0002 0.0041 0.0003 0.0002 0.0001 0.0001 0.0001 – – – – – –
Endoplasmic Reticulum 0.0038 0.0004 0.0003 0.0003 0.0011 0.0006 0.0076 0.0002 0.0002 0.0002 0.0002 0.0009 – – – – – –
Endosome 0.0298 0.0034 0.0026 0.0002 0.0393 0.0048 0.0662 0.0001 0.0008 0.0002 0.0057 0.0003 – – – – – –
Golgi 0.076 0.0032 0.0051 0 0.0963 0.0101 0.0306 0 0.0005 0 0.0016 0.0001 – – – – – –
Lipid Particles 0.0157 0.0002 0.0012 0.0001 0.0865 0.0012 0.0158 0.0001 0.0011 0.0001 0.0084 0.0002 – – – – – –
Mitochondria 0.0115 0.0091 0.0022 0.0002 0.0165 0.0062 0.0101 0.0003 0.0005 0.0001 0.0006 0.0003 – – – – – –
None 0.0009 0.947 0.9304 0.9658 0.6571 0.8939 0.4753 0.9681 0.9177 0.9752 0.8815 0.9348 – – – – – –
Nuclear Periphery 0.0006 0.0002 0.0006 0.0002 0.0007 0.0008 0.0046 0.0002 0.0004 0.0003 0.0002 0.0011 – – – – – –
Nucleolus 0.0001 0.0001 0.001 0 0.0002 0.0001 0.0026 0.0003 0.0005 0.0003 0.0001 0.0003 – – – – – –
Nucleus 0.0003 0.0003 0.0032 0.0002 0.0002 0.0009 0.0026 0.0015 0.0013 0.0005 0.0004 0.0016 – – – – – –
Peroxisomes 0.0308 0.0003 0.0083 0.0001 0.0123 0.0007 0.0771 0.0002 0.0029 0.0002 0.0331 0.0003 – – – – – –
Punctate Nuclear 0.0014 0.0002 0.0109 0.0002 0.0013 0.0016 0.0193 0.0024 0.0034 0.0049 0.0014 0.0086 – – – – – –
Vacuole 0.0005 0.0003 0.0003 0.0001 0.001 0.0003 0.009 0.0001 0.0003 0.0001 0.0001 0.0002 – – – – – –
Vacuole Periphery 0.0003 0.0001 0.0001 0 0.0008 0.0002 0.0014 0 0.0001 0 0 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5387 6.405 11.0899 13.5179 6.1931 8.3545 7.0158 14.2056 9.8869 15.11
Translational Efficiency 1.6135 1.9255 1.0856 0.5204 1.8359 1.8511 2.0366 0.764 1.4541 1.218

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
149 32 139 1768 198 1638 1797 45 347 1670 1936 1813

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 675.24 771.38 667.10 683.24 640.89 779.92 704.83 1316.63 655.64 779.76 702.12 698.96
Standard Deviation 96.67 142.66 91.51 80.15 77.56 95.30 83.58 1933.63 87.95 96.44 84.73 329.82
Intensity Change Log 2 — 0.192041 -0.017497 0.016992 — 0.283249 0.137199 1.038701 — 0.237176 0.059905 0.603604

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000600 0.000121 0.000156 0.000133 0.000261 0.000082 0.001252 0.000098 0.000268 0.000084 0.000183
Bud Neck 0.008193 0.020749 0.008435 0.004835 0.009383 0.025813 0.005804 0.018597 0.008872 0.025716 0.005993 0.005177
Bud Site 0.001013 0.043711 0.010401 0.006239 0.005750 0.019945 0.005383 0.075422 0.003716 0.020400 0.005743 0.007957
Cell Periphery 0.000136 0.000265 0.000211 0.000205 0.000194 0.000216 0.000159 0.008667 0.000169 0.000217 0.000163 0.000415
Cytoplasm 0.467691* 0.384444* 0.375467* 0.368895* 0.465311* 0.527392* 0.344182* 0.353423* 0.466333* 0.524653* 0.346428* 0.368511*
Cytoplasmic Foci 0.136940 0.034265 0.046401 0.020764 0.168137 0.172556 0.017830 0.070268 0.154741 0.169906 0.019881 0.021992
Eisosomes 0.000127 0.000068 0.000077 0.000039 0.000275 0.000253 0.000034 0.000082 0.000212 0.000250 0.000037 0.000040
Endoplasmic Reticulum 0.000431 0.002131 0.001366 0.001828 0.000401 0.000487 0.001027 0.000799 0.000414 0.000519 0.001052 0.001802
Endosome 0.006660 0.013683 0.004762 0.003539 0.005285 0.005121 0.002621 0.024706 0.005875 0.005285 0.002775 0.004064
Golgi 0.000343 0.001747 0.000118 0.000381 0.000515 0.001539 0.000254 0.019372 0.000441 0.001543 0.000244 0.000852
Lipid Particles 0.003478 0.015695 0.003336 0.001462 0.003177 0.001805 0.001318 0.008144 0.003307 0.002071 0.001463 0.001628
Mitochondria 0.001052 0.000850 0.000448 0.001761 0.004230 0.001073 0.000511 0.010128 0.002865 0.001068 0.000506 0.001969
Mitotic Spindle 0.000531 0.016539 0.001687 0.003738 0.000044 0.003029 0.005013 0.013095 0.000253 0.003288 0.004774 0.003970
None 0.002904 0.010651 0.016540 0.004298 0.001199 0.002485 0.006167 0.004256 0.001931 0.002641 0.006912 0.004297
Nuclear Periphery 0.000570 0.000850 0.001076 0.001785 0.000365 0.000177 0.001425 0.000905 0.000453 0.000190 0.001400 0.001763
Nuclear Periphery Foci 0.000241 0.001326 0.000539 0.000529 0.000196 0.000153 0.000260 0.001176 0.000215 0.000175 0.000280 0.000545
Nucleolus 0.000839 0.004120 0.001616 0.000879 0.000298 0.000446 0.000920 0.001369 0.000530 0.000517 0.000970 0.000891
Nucleus 0.272008* 0.288553* 0.482677* 0.545656* 0.238926* 0.152997* 0.584758* 0.253726* 0.253131* 0.155594* 0.577428* 0.538410*
Peroxisomes 0.006468 0.025181 0.000544 0.000636 0.002590 0.003573 0.000528 0.005164 0.004255 0.003987 0.000529 0.000748
Vacuole 0.090149 0.134238 0.043784 0.031800 0.093345 0.080333 0.021513 0.124134 0.091972 0.081366 0.023112 0.034092
Vacuole Periphery 0.000176 0.000334 0.000395 0.000576 0.000247 0.000345 0.000212 0.005315 0.000216 0.000345 0.000225 0.000693

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.53 -2.98 -3.24 2.93 -0.13 -2.18 1.09 -1.65 -1.38 -1.71 -3.07 -0.06 -2.37 1.41 -2.55
Bud Neck -0.72 0.02 1.02 1.17 0.69 -5.40 1.06 -0.73 1.28 -1.13 -6.82 1.19 1.51 12.24 0.55
Bud Site -1.95 -1.80 -4.20 1.63 0.86 -5.24 0.27 -1.64 -1.15 -1.66 -7.25 -1.25 -1.93 5.54 -0.89
Cell Periphery -1.56 -3.14 -2.23 0.32 0.59 -0.87 1.72 -1.26 -1.25 -1.27 -2.18 0.49 -1.43 -1.04 -1.50
Cytoplasm 1.76 3.06 4.41 0.07 -0.09 -3.69 6.20 2.47 4.35 -0.18 -4.34 7.81 6.43 18.60 -2.25
Cytoplasmic Foci 6.19 6.65 9.61 1.96 3.49 -0.52 11.77 4.35 5.51 -2.84 -1.86 14.97 14.69 33.34 -1.39
Eisosomes 2.62 2.75 6.64 2.28 4.83 0.43 6.47 4.49 7.54 -3.85 -1.55 7.81 7.65 14.19 -1.52
Endoplasmic Reticulum -2.88 -4.08 -6.71 0.19 -0.01 -0.18 -8.44 -2.35 -2.09 -0.26 -0.19 -9.45 -7.52 -5.61 -3.12
Endosome -0.57 0.83 1.98 1.34 1.33 0.05 1.80 -1.25 -1.26 -1.52 0.55 2.70 1.76 2.04 -1.33
Golgi -1.09 1.26 -0.01 1.10 -1.37 -2.13 0.95 -1.35 -1.24 -1.38 -2.45 0.98 -0.74 1.73 -1.38
Lipid Particles -0.88 -0.18 2.05 1.12 3.55 2.73 3.75 -1.61 -2.11 -2.25 2.43 3.48 3.25 0.91 -0.61
Mitochondria 0.62 0.99 -1.00 -2.36 -3.07 0.79 0.92 -0.42 -0.98 -1.07 0.77 1.01 0.43 -1.68 -3.08
Mitotic Spindle -1.25 -1.23 -4.05 0.79 -2.42 -2.78 -4.66 -1.15 -0.75 -0.24 -2.69 -4.53 -4.94 -1.10 1.16
None -1.20 -2.07 -1.41 0.87 1.86 -3.07 -7.67 -2.41 -1.19 1.99 -1.48 -6.25 -3.58 -2.62 3.19
Nuclear Periphery -0.79 -3.22 -7.62 -6.41 -4.05 4.31 -18.81 -4.34 -5.53 3.17 4.11 -13.68 -11.35 -14.91 -2.87
Nuclear Periphery Foci -3.36 -2.73 -1.89 2.41 0.46 1.98 -2.66 -2.69 -2.86 -2.34 2.40 -2.43 -2.56 -3.19 -1.62
Nucleolus -1.04 -2.42 -0.18 1.02 2.62 -2.83 -8.44 -1.98 -1.57 -0.43 0.02 -4.55 -2.62 -2.91 1.20
Nucleus 0.19 -6.11 -12.63 -5.22 -2.01 4.72 -17.91 -0.90 -3.07 7.29 6.78 -21.49 -18.52 -46.15 4.14
Peroxisomes -0.82 1.31 1.29 1.34 -0.44 -1.33 3.47 -0.53 -0.05 -1.51 0.08 1.94 1.85 5.33 -0.87
Vacuole -2.44 2.30 4.59 3.82 1.74 2.08 7.09 -1.77 -2.64 -4.44 2.41 8.75 6.66 9.46 -5.54
Vacuole Periphery -1.52 -1.67 -2.34 -1.67 -0.25 -0.85 0.20 -1.71 -1.66 -1.72 -1.68 -0.23 -2.37 -1.68 -2.45
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
Localization
Cell Percentages nucleus (15%), cytoplasm (6%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Srb5

Srb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available