Standard name
Human Ortholog
Description Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – – 0 0 0 0
Cytoplasm – – 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.15 – – – – 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 – – – – 0 0 0 0
Endosome – – 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.05 0.06 – – – – 0 0 0.06 0.06
Golgi – – 0 0 0 0 0 0 0 0 0 0 0.24 0.5 0.36 0.39 – – – – 0 0.05 0.08 0.12
Mitochondria – – 0.94 0.91 0.88 0.92 0.92 0.89 0.92 0.94 0.94 0.96 0.69 0.57 0.62 0.12 – – – – 0.78 0.76 0.67 0.65
Nucleus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0 0 0 0
Nuclear Periphery – – 0 0 0 0.11 0.09 0.07 0.08 0.07 0.08 0.1 0 0 0 0.06 – – – – 0 0 0 0
Nucleolus – – 0 0 0.05 0.08 0.08 0.09 0.09 0.1 0.07 0 0 0 0 0.09 – – – – 0 0 0 0
Peroxisomes – – 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0.12 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – – 0 0 0 0
Vac/Vac Membrane – – 0 0 0.08 0.06 0.05 0 0.05 0 0.06 0.05 0.19 0.2 0.14 0.21 – – – – 0 0.06 0.08 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 3 0 2 0 1 0 0 0 0 1 2 1 6 0 – – – – 4 3 1 5
Bud – – 14 10 7 9 18 15 20 19 16 22 2 2 1 0 – – – – 0 0 1 1
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – 0 0 0 1
Bud Site – – 2 2 1 7 14 11 4 5 8 8 0 0 0 0 – – – – – – – –
Cell Periphery – – 13 10 3 2 4 0 2 6 5 6 8 10 5 2 – – – – 0 0 0 1
Cytoplasm – – 4 9 6 12 16 7 7 3 7 2 9 11 9 5 – – – – 0 1 3 3
Endoplasmic Reticulum – – 1 0 1 4 3 1 5 0 4 1 6 3 11 2 – – – – 2 2 0 2
Endosome – – 17 2 20 0 1 2 3 0 1 0 11 8 13 2 – – – – 12 7 13 16
Golgi – – 3 2 0 0 0 0 1 0 0 1 47 117 88 13 – – – – 13 10 20 34
Mitochondria – – 402 208 281 439 611 410 417 414 483 496 136 134 150 4 – – – – 331 164 165 186
Nucleus – – 2 0 3 6 5 7 3 4 6 4 1 0 0 0 – – – – 0 3 1 1
Nuclear Periphery – – 13 3 10 54 63 32 34 31 41 51 0 2 6 2 – – – – 1 0 2 2
Nucleolus – – 9 6 15 39 53 42 43 45 38 19 0 2 1 3 – – – – 0 1 2 2
Peroxisomes – – 34 11 11 0 8 14 2 2 0 3 3 1 5 1 – – – – 51 4 6 10
SpindlePole – – 12 5 7 6 3 10 10 3 7 12 3 4 2 2 – – – – 2 0 1 1
Vac/Vac Membrane – – 14 10 27 27 32 13 24 18 31 26 37 47 34 7 – – – – 1 12 20 13
Unique Cell Count 426 228 318 475 664 460 453 440 514 515 196 235 242 33 427 217 246 286
Labelled Cell Count 543 278 394 605 832 564 575 550 647 652 265 342 331 43 427 217 246 286


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 6.3 6.1 6.4 5.2 5.1 5.2 5.1 4.9 4.7 4.6 7.6 7.9 7.5 13.5 – – 7.5 8.4 8.7
Std Deviation (1e-4) – – 1.5 2.0 1.9 1.4 1.4 1.6 1.4 1.2 1.4 1.3 1.1 1.2 1.2 3.7 – – 1.5 1.7 1.8
Intensity Change (Log2) – – – -0.05 0.03 -0.28 -0.3 -0.26 -0.29 -0.34 -0.4 -0.44 0.29 0.34 0.26 1.11 – – 0.27 0.42 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0.7 -0.9 -1.3 -0.5 0 0.9 0.8 0 0 -1.7 -2.0 -2.4 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 2.2 2.4 0 0 0 0 0 0 0 0 – –
Cell Periphery 0.9 -2.0 -3.1 -3.2 -3.8 -3.0 -1.7 0 0 0.7 0.8 -0.8 0 – –
Cytoplasm 0 0 1.8 1.8 0.8 0.8 0 0 0 0 3.1 0 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome -2.3 1.4 -4.4 -4.9 -3.7 -3.3 -4.2 0 0 0.9 -0.4 0.8 0 – –
Golgi 0 0 0 0 0 0 0 0 0 9.9 15.7 12.9 0 – –
Mitochondria -1.5 -3.0 -1.2 -1.5 -2.8 -1.4 -0.2 0 0 -8.5 -11.7 -10.6 -14.2 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery -1.4 0.1 4.8 4.1 2.6 2.9 2.7 0 0 0 -1.8 -0.4 0 – –
Nucleolus 0.4 2.0 4.1 4.1 4.5 4.6 4.9 0 0 0 0 0 0 – –
Peroxisomes -1.5 -2.6 -6.3 -5.7 -3.2 -5.6 -5.5 0 0 -3.2 -4.2 -3.1 0 – –
SpindlePole -0.5 -0.5 -1.7 -3.3 -0.6 -0.6 -2.4 0 0 0 -0.9 -1.7 0 – –
Vacuole 0.7 3.1 1.7 1.2 -0.4 1.5 0.6 2.0 1.3 6.6 7.1 5.2 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8377 2.342 1.7035 1.1669 1.2487 2.115 2.276 2.3437 2.4438 1.7828 2.9142 2.5536 2.5006 2.5829 2.4232 2.323 1.7125 2.6324
Actin 0.0018 0.0039 0.0079 0.0001 0.0006 0.0006 0.089 0.0073 0.0125 0.0153 0.0271 0.007 0.0122 0.0008 0.0071 0.0349 0.0127 0.0018
Bud 0.0002 0.0053 0.0006 0.0001 0.003 0.0003 0.0006 0.0019 0.0003 0.0012 0.0033 0.001 0.0007 0.0003 0.0007 0.0011 0.0031 0.0008
Bud Neck 0.0019 0.0002 0.0003 0.0002 0.0001 0.0001 0.0016 0.0005 0.0003 0.0004 0.001 0.0005 0.0004 0.0002 0.0006 0.0008 0.0015 0.0006
Bud Periphery 0.0005 0.0075 0.0017 0.0007 0.0132 0.0008 0.0012 0.0067 0.0005 0.0025 0.0059 0.0039 0.0013 0.0006 0.0009 0.0017 0.0032 0.001
Bud Site 0.0051 0.0034 0.0048 0.0002 0.0005 0.0001 0.0045 0.0107 0.0009 0.0034 0.0033 0.0009 0.0013 0.0013 0.0107 0.0005 0.0013 0.0005
Cell Periphery 0.0004 0.0004 0.0003 0.0004 0.0001 0.0001 0.0003 0.0077 0.0003 0.0005 0.0007 0.0015 0.0002 0.0004 0.0004 0.0004 0.0003 0.0001
Cytoplasm 0.0008 0.0002 0.0009 0.0001 0.0002 0.0001 0.0086 0.0008 0.0043 0.0051 0.0102 0.001 0.0142 0.0006 0.0131 0.0602 0.0262 0.0139
Cytoplasmic Foci 0.0117 0.0093 0.0081 0.0015 0.0103 0.003 0.0255 0.0081 0.0198 0.0264 0.0166 0.0078 0.0164 0.0046 0.014 0.0104 0.0057 0.0058
Eisosomes 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0007 0.0003 0.0003 0.0005 0.0002 0.0002 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0002 0.0001 0.0002 0 0 0 0.003 0.0003 0.0014 0.0008 0.0038 0.0005 0.0021 0.0004 0.0008 0.0009 0.0011 0.0005
Endosome 0.0335 0.0304 0.016 0.0032 0.0131 0.0077 0.0451 0.0438 0.064 0.0456 0.0494 0.0133 0.0537 0.0334 0.0404 0.0114 0.0432 0.0284
Golgi 0.0215 0.0459 0.0141 0.0027 0.0267 0.0131 0.0352 0.0256 0.0337 0.0307 0.043 0.0219 0.0307 0.04 0.038 0.0086 0.0469 0.0256
Lipid Particles 0.0256 0.0092 0.0095 0.0004 0.0061 0.0107 0.0167 0.0072 0.0093 0.0097 0.0638 0.0095 0.02 0.003 0.0107 0.0058 0.007 0.0073
Mitochondria 0.8432 0.8468 0.8641 0.9833 0.8694 0.9418 0.6869 0.848 0.8109 0.7956 0.6616 0.8753 0.7828 0.8674 0.8095 0.8201 0.802 0.8749
None 0.0052 0.0001 0.0098 0.0001 0.0003 0 0.0073 0.0007 0.0005 0.024 0.001 0.0003 0.0014 0.0001 0.0007 0.0003 0.0004 0.0005
Nuclear Periphery 0.0001 0.0001 0.0023 0 0 0 0.0136 0.0001 0.0006 0.001 0.009 0.0095 0.0035 0.0005 0.001 0.0007 0.002 0.0021
Nucleolus 0.0004 0.0002 0.0007 0.0001 0.0002 0.0004 0.0012 0.0023 0.0001 0.0008 0.0009 0.0004 0.0003 0.002 0.0006 0.0009 0.0003 0.0004
Nucleus 0.0001 0.0002 0.001 0.0001 0.0001 0 0.0167 0.0005 0.0002 0.0011 0.0144 0.0094 0.0098 0.021 0.017 0.001 0.0042 0.0074
Peroxisomes 0.0414 0.0322 0.0496 0.0048 0.0548 0.0189 0.0167 0.0142 0.023 0.0273 0.0156 0.0241 0.0278 0.0057 0.0237 0.0187 0.0232 0.0128
Punctate Nuclear 0.0002 0 0.0004 0 0 0 0.013 0.0001 0.0005 0.0041 0.0013 0.0055 0.0022 0 0.0012 0.0003 0.0013 0.0013
Vacuole 0.0033 0.0026 0.004 0.0008 0.0006 0.0011 0.0046 0.0093 0.0082 0.0018 0.0453 0.004 0.0074 0.0087 0.0051 0.0074 0.0062 0.0088
Vacuole Periphery 0.0028 0.002 0.0034 0.0012 0.0006 0.0012 0.008 0.004 0.0085 0.0022 0.0226 0.0024 0.0113 0.0087 0.0036 0.0137 0.0081 0.0053

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5446 5.9014 5.6303 8.4304 6.7251 3.7702 10.229 8.9175 10.9614 9.5264
Translational Efficiency 0.8359 0.9091 0.8666 0.849 0.7667 1.7944 0.5928 0.7453 0.769 0.7416

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1893 77 210 1668 1921 1858 1319 1128 3814 1935 1529 2796

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 836.45 1023.35 1076.86 1008.08 828.92 901.66 1025.27 1055.13 832.66 906.50 1032.36 1027.06
Standard Deviation 105.74 148.47 125.00 152.08 111.09 136.65 134.63 170.88 108.54 139.19 134.53 161.59
Intensity Change Log 2 — 0.290948 0.364479 0.269259 — 0.121351 0.306699 0.348116 — 0.209023 0.336009 0.309048

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000494 0.007697 0.004471 0.006445 0.000664 0.001666 0.003725 0.013031 0.000579 0.001906 0.003827 0.009102
Bud Neck 0.000828 0.002137 0.009399 0.001221 0.000681 0.001077 0.003963 0.002532 0.000754 0.001120 0.004709 0.001750
Bud Site 0.002512 0.010722 0.029254 0.020646 0.002486 0.002999 0.025386 0.031114 0.002499 0.003306 0.025918 0.024869
Cell Periphery 0.000328 0.000296 0.000486 0.000864 0.000277 0.000220 0.000401 0.001073 0.000302 0.000223 0.000413 0.000949
Cytoplasm 0.000364 0.001478 0.001856 0.003151 0.000409 0.001762 0.004019 0.004266 0.000387 0.001751 0.003722 0.003601
Cytoplasmic Foci 0.010737 0.064889 0.082486 0.020969 0.015180 0.023692 0.047677 0.024354 0.012975 0.025332 0.052458 0.022335
Eisosomes 0.000029 0.000091 0.000075 0.000087 0.000034 0.000044 0.000069 0.000124 0.000032 0.000046 0.000070 0.000102
Endoplasmic Reticulum 0.000341 0.000491 0.001105 0.000727 0.000283 0.000233 0.001383 0.000759 0.000312 0.000243 0.001345 0.000740
Endosome 0.032962 0.075963 0.120378 0.030766 0.033197 0.038191 0.073697 0.037840 0.033080 0.039694 0.080108 0.033620
Golgi 0.026010 0.076184 0.099529 0.071303 0.034694 0.037956 0.084217 0.082435 0.030384 0.039477 0.086320 0.075794
Lipid Particles 0.001576 0.008888 0.003032 0.000230 0.003236 0.002697 0.002069 0.000308 0.002412 0.002943 0.002201 0.000261
Mitochondria 0.882029* 0.676157* 0.425911* 0.775440* 0.867321* 0.845273* 0.589894* 0.728538* 0.874621* 0.838543* 0.567372* 0.756518*
Mitotic Spindle 0.002896 0.013887 0.083079 0.020866 0.002783 0.003470 0.053452 0.016236 0.002839 0.003884 0.057522 0.018998
None 0.000173 0.000212 0.000251 0.000392 0.000331 0.000355 0.000354 0.000353 0.000253 0.000349 0.000340 0.000376
Nuclear Periphery 0.000247 0.000231 0.001852 0.000070 0.000188 0.000205 0.000526 0.000074 0.000217 0.000206 0.000708 0.000072
Nuclear Periphery Foci 0.000217 0.002289 0.001695 0.000108 0.000246 0.000378 0.000669 0.000135 0.000232 0.000454 0.000810 0.000119
Nucleolus 0.000157 0.000072 0.000382 0.000060 0.000233 0.000215 0.000930 0.000056 0.000195 0.000209 0.000855 0.000059
Nucleus 0.000151 0.000120 0.000636 0.000062 0.000176 0.000780 0.001077 0.000078 0.000163 0.000754 0.001016 0.000068
Peroxisomes 0.015925 0.044295 0.041377 0.019566 0.013339 0.013160 0.037577 0.023304 0.014622 0.014399 0.038099 0.021074
Vacuole 0.007269 0.009195 0.048385 0.011535 0.008162 0.011939 0.032960 0.015197 0.007719 0.011830 0.035078 0.013012
Vacuole Periphery 0.014756 0.004705 0.044362 0.015492 0.016080 0.013688 0.035955 0.018193 0.015423 0.013330 0.037110 0.016582

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.77 -3.68 -6.21 0.30 -1.38 -1.72 -3.99 -6.05 -5.36 -4.26 -2.29 -4.79 -8.48 -6.23 -4.35
Bud Neck -1.56 -3.53 -1.98 1.09 3.37 -1.73 -7.18 -3.50 -2.62 2.23 -1.71 -7.69 -4.11 -2.12 5.41
Bud Site -1.25 -4.00 -9.55 -1.46 1.24 -0.73 -9.23 -9.85 -9.61 -1.44 -1.20 -10.11 -13.72 -12.62 0.47
Cell Periphery 0.30 -0.73 -3.69 -3.63 -1.54 0.82 -0.99 -6.03 -7.19 -4.25 1.54 -1.03 -6.37 -7.67 -4.02
Cytoplasm -1.93 -4.95 -11.83 -2.71 -3.49 -2.39 -5.15 -6.16 -2.78 -0.15 -2.50 -5.50 -11.12 -2.71 0.31
Cytoplasmic Foci -3.77 -8.88 -6.85 3.05 7.55 -4.45 -13.21 -4.38 -0.23 8.01 -6.86 -16.85 -7.58 1.51 12.06
Eisosomes -3.14 -6.86 -10.29 0.19 -1.41 -3.58 -10.37 -9.61 -8.34 -5.67 -5.34 -12.97 -13.92 -10.14 -5.72
Endoplasmic Reticulum -0.57 -3.68 -2.64 -0.90 1.86 1.17 -4.54 -5.04 -5.77 2.47 1.10 -4.79 -4.81 -6.74 2.78
Endosome -2.92 -8.56 0.86 3.08 8.83 -1.80 -11.04 -1.59 0.11 9.32 -2.70 -14.02 -0.30 2.50 13.95
Golgi -3.19 -7.48 -12.14 0.30 2.75 -1.09 -11.25 -9.44 -8.62 0.27 -3.52 -14.47 -14.99 -10.28 2.28
Lipid Particles -1.11 -2.06 4.24 1.31 4.38 0.74 1.41 4.76 5.65 3.27 -0.89 0.33 6.17 5.54 4.08
Mitochondria 4.68 17.45 12.04 -2.23 -13.10 2.79 24.41 12.40 10.08 -9.90 5.12 30.23 17.01 9.67 -16.96
Mitotic Spindle -1.96 -6.23 -9.54 -1.21 4.79 -0.95 -14.18 -7.39 -6.74 9.39 -1.43 -15.35 -12.13 -10.49 10.22
None -1.30 -3.68 -6.41 -4.21 -3.79 -0.23 -0.19 -0.14 0.23 0.20 -1.16 -1.19 -1.64 -0.25 -1.01
Nuclear Periphery 0.08 -3.09 1.98 1.03 3.48 -0.30 -3.50 2.45 3.41 5.18 0.18 -4.31 2.92 3.81 6.18
Nuclear Periphery Foci -0.97 -4.03 1.76 1.02 4.37 -0.94 -2.88 1.33 2.07 4.25 -1.51 -4.52 2.16 2.38 5.79
Nucleolus 1.34 -1.85 0.72 -0.38 2.32 0.09 -2.00 1.24 3.11 2.57 -0.20 -2.23 1.35 2.23 2.61
Nucleus 0.46 -3.11 1.90 1.12 3.82 -1.75 -3.34 1.47 2.02 3.74 -1.79 -3.66 2.33 2.05 4.06
Peroxisomes -1.99 -3.22 -1.41 1.72 2.72 0.08 -7.03 -3.31 -3.38 3.49 0.11 -7.53 -3.33 -3.11 5.11
Vacuole -0.65 -6.37 -3.41 -0.75 5.65 -3.20 -12.27 -4.05 -1.81 7.28 -4.08 -14.32 -5.15 -0.95 10.72
Vacuole Periphery 6.67 -4.99 -0.45 -6.98 4.86 1.61 -8.03 -0.98 -2.28 6.35 1.79 -9.92 -0.89 -2.50 9.08
Standard name
Human Ortholog
Description Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Shy1

Shy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Shy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available