ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.16 0.14 0.11 0.13 0.2 0.18 – 0.21 0.22 0.19 0.22 0.23 0.22 0.27 0.21 0.2 0.22 0.12 0.06 0.1 0.13 0.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0 – 0.1 0.08 0.13 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.61 0.74 0.75 0.85 0.75 0.63 0.61 – 0.52 0.54 0.56 0.52 0.61 0.71 0.57 0.54 0.41 0.41 0.47 0.56 0.5 0.37 0.33 0.37
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.24 0.13 0.06 0.21 0.17 0.28 – 0.26 0.23 0.22 0.2 0.22 0.12 0.22 0.32 0.43 0.4 0.3 0.3 0.3 0.39 0.4 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 2 1 1 1 0 0 0 0 1 0 0 1 1 0 1 1
Bud 0 0 0 0 0 1 0 – 4 4 4 4 0 0 0 0 0 0 8 9 3 12 21 23
Bud Neck 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 1 2 3 5 17 17
Bud Site 0 0 0 0 0 2 0 – 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 2 1 – 1 1 2 1 5 4 10 0 0 3 0 0 0 0 0 0
Cytoplasm 22 41 21 31 48 61 76 – 83 94 67 75 67 77 109 51 22 28 30 17 17 28 53 39
Endoplasmic Reticulum 0 4 0 2 1 2 1 – 1 5 1 2 3 5 1 11 6 6 7 0 2 3 12 12
Endosome 5 0 1 1 1 0 0 – 2 4 3 1 1 0 1 7 3 4 2 3 1 1 4 4
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 1 1 1 0 0 0 1 1 0 2 4
Mitochondria 2 5 2 0 0 25 5 – 38 33 46 51 1 0 0 3 0 2 1 0 2 1 4 3
Nucleus 129 189 112 231 284 194 265 – 206 229 200 175 182 244 231 130 45 53 122 165 87 84 175 141
Nuclear Periphery 3 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 0 0 2 0 0 0 5 3
Nucleolus 1 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 5
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 0 1 0 0 6 4
Vac/Vac Membrane 72 62 20 17 79 52 120 – 102 97 79 68 65 43 89 77 47 52 77 86 52 90 214 118
Unique Cell Count 210 255 149 272 377 310 431 395 426 359 335 296 344 406 242 110 129 261 294 177 231 533 383
Labelled Cell Count 234 301 157 282 413 340 468 439 474 404 379 324 375 442 281 124 148 261 294 177 231 533 383


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.9 7.1 7.3 8.3 6.1 7.9 – 7.4 7.3 7.3 7.3 8.3 7.5 8.7 11.1 12.3 12.3 7.2 7.3 7.7
Std Deviation (1e-4) 1.3 0.8 0.9 1.3 1.5 1.3 1.4 – 1.6 1.3 1.3 1.2 1.1 1.3 1.5 1.9 2.1 2.0 1.4 1.3 1.5
Intensity Change (Log2) – – – 0.04 0.22 -0.21 0.15 – 0.06 0.05 0.04 0.04 0.23 0.07 0.3 0.65 0.79 0.79 0.01 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -0.4 1.5 1.0 – 1.8 2.1 1.2 2.1 2.1 2.1 3.1 1.7 1.3 1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.9 0 – 3.3 2.8 4.0 4.5 0 0 0 0 0 0
Nucleus 2.5 0 -2.7 -3.0 – -4.9 -4.6 -4.1 -4.7 -2.9 -1.0 -3.9 -4.2 -5.6 -5.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -2.5 2.0 0.9 3.5 – 3.1 2.4 2.2 1.8 2.2 -0.3 2.2 4.1 5.3 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6955 2.0536 1.9032 1.6659 0.7468 1.8534 3.3375 3.7226 3.4586 3.1898 2.7335 3.0915 5.3296 6.4496 5.325 5.738 5.4512 5.4875
Actin 0.0115 0.0003 0.0032 0.0002 0.0036 0.0019 0.0351 0.0011 0.0006 0.0132 0.0002 0.0029 0.0522 0.0005 0.014 0.0002 0.0038 0.0035
Bud 0.0003 0.0002 0.0001 0.0001 0.0005 0.0002 0.0006 0.0073 0.0026 0.0004 0.0003 0.0003 0.0009 0.0003 0.0007 0.0001 0.0002 0.0004
Bud Neck 0.005 0.0017 0.0011 0.0031 0.0037 0.0147 0.009 0.0028 0.0019 0.0058 0.0108 0.0158 0.0037 0.0012 0.0047 0.0009 0.0019 0.0202
Bud Periphery 0.0004 0.0001 0.0001 0 0.0008 0.0002 0.001 0.0014 0.0015 0.0003 0.0002 0.0003 0.0027 0.0002 0.0008 0 0.0002 0.0007
Bud Site 0.0016 0.003 0.0009 0.0003 0.0021 0.0004 0.0034 0.005 0.0078 0.0012 0.0017 0.0006 0.0027 0.0039 0.0047 0.0003 0.0009 0.001
Cell Periphery 0.0004 0.0003 0.0002 0.0001 0.0003 0.0002 0.0018 0.001 0.0005 0.0004 0.0005 0.0004 0.0011 0.0003 0.0004 0.0001 0.0001 0.001
Cytoplasm 0.2276 0.2362 0.1867 0.2141 0.0932 0.1618 0.3419 0.2887 0.2639 0.2175 0.405 0.2978 0.221 0.176 0.2132 0.207 0.1356 0.1744
Cytoplasmic Foci 0.0114 0.0014 0.0049 0.0004 0.0108 0.0023 0.0296 0.0024 0.0031 0.0012 0.0005 0.0014 0.0113 0.0007 0.0072 0.0007 0.0104 0.0019
Eisosomes 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0.0002 0 0.0001 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0157 0.0153 0.0143 0.0063 0.0076 0.0084 0.0273 0.0167 0.0064 0.004 0.0075 0.021 0.0122 0.0136 0.0074 0.0071 0.0095 0.0107
Endosome 0.0232 0.0031 0.0092 0.0008 0.0615 0.0032 0.0748 0.0027 0.0155 0.002 0.0008 0.004 0.0044 0.0014 0.0024 0.001 0.0174 0.0073
Golgi 0.0046 0.0001 0.003 0 0.015 0.0009 0.0153 0.0004 0.0041 0.0067 0 0.0056 0.0027 0.0001 0.0012 0 0.0093 0.0016
Lipid Particles 0.0162 0.0003 0.0081 0.0001 0.0311 0.0023 0.0182 0.0004 0.0005 0.0004 0.0001 0.0033 0.0041 0.0003 0.0005 0 0.0082 0.0008
Mitochondria 0.0048 0.0003 0.0016 0.0001 0.0097 0.0007 0.0029 0.0038 0.0038 0.0275 0.0002 0.0231 0.0134 0.0002 0.0013 0.0002 0.0122 0.0014
None 0.0188 0.0086 0.0078 0.0441 0.042 0.0076 0.0085 0.005 0.0039 0.0002 0.0004 0.0005 0.0191 0.0489 0.0168 0.0208 0.049 0.012
Nuclear Periphery 0.0418 0.0392 0.0392 0.0099 0.0446 0.018 0.0298 0.0263 0.0221 0.0137 0.0166 0.0163 0.0176 0.0208 0.0136 0.0145 0.012 0.0114
Nucleolus 0.0026 0.0021 0.0013 0.0006 0.003 0.002 0.0015 0.0035 0.0011 0.0008 0.0012 0.0018 0.001 0.0016 0.0011 0.0006 0.0016 0.0017
Nucleus 0.5698 0.6765 0.7062 0.716 0.6384 0.7582 0.3522 0.6091 0.6495 0.6997 0.5451 0.5966 0.603 0.7226 0.7007 0.742 0.6933 0.738
Peroxisomes 0.0196 0.0001 0.0018 0 0.0053 0.0021 0.011 0.0024 0.001 0.001 0 0.001 0.0152 0 0.0024 0 0.0256 0.0001
Punctate Nuclear 0.0159 0.0051 0.0064 0.0019 0.0128 0.0089 0.0075 0.0084 0.0022 0.0013 0.0029 0.0022 0.0028 0.0023 0.0037 0.0027 0.0051 0.0028
Vacuole 0.0072 0.0056 0.0032 0.0017 0.0113 0.0057 0.0266 0.0112 0.0072 0.0023 0.0056 0.0047 0.008 0.0049 0.0031 0.0016 0.003 0.0084
Vacuole Periphery 0.0014 0.0005 0.0006 0.0001 0.0027 0.0002 0.0017 0.0003 0.0008 0.0002 0.0002 0.0004 0.0005 0.0002 0.0002 0.0001 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7572 4.27 3.1685 7.8676 8.2575 1.5913 8.9946 7.2849 10.901 8.6343
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2313 1498 2604 2063 1596 2578 1218 1787 3909 4076 3822 3850

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 846.27 924.40 1133.47 1247.64 789.68 929.65 1137.10 1279.43 823.16 927.72 1134.63 1262.40
Standard Deviation 107.30 127.23 171.98 223.07 109.18 129.37 179.75 218.95 111.60 128.61 174.50 221.74
Intensity Change Log 2 — 0.127399 0.421556 0.560012 — 0.235420 0.526019 0.696161 — 0.180551 0.472926 0.627337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000144 0.000560 0.000289 0.000871 0.000367 0.000290 0.001012 0.000980 0.000235 0.000389 0.000519 0.000921
Bud Neck 0.003537 0.006614 0.012243 0.032919 0.002216 0.010163 0.019519 0.027527 0.002998 0.008859 0.014562 0.030416
Bud Site 0.001071 0.003761 0.002432 0.014646 0.001540 0.004564 0.006724 0.010141 0.001263 0.004269 0.003800 0.012555
Cell Periphery 0.000487 0.000550 0.000318 0.000893 0.000401 0.000349 0.000892 0.000527 0.000452 0.000423 0.000501 0.000723
Cytoplasm 0.124755* 0.139091* 0.049613 0.063738 0.150193* 0.139240* 0.033420 0.072212 0.135141* 0.139185* 0.044453 0.067671
Cytoplasmic Foci 0.009805 0.008887 0.000174 0.001603 0.014907 0.006338 0.000733 0.001711 0.011888 0.007275 0.000352 0.001653
Eisosomes 0.000035 0.000042 0.000018 0.000064 0.000047 0.000023 0.000077 0.000047 0.000040 0.000030 0.000037 0.000056
Endoplasmic Reticulum 0.002898 0.013093 0.004112 0.014230 0.003530 0.003708 0.020369 0.011431 0.003156 0.007157 0.009293 0.012931
Endosome 0.000631 0.001413 0.000228 0.001657 0.001132 0.001504 0.001221 0.002530 0.000836 0.001471 0.000544 0.002062
Golgi 0.000318 0.000483 0.000026 0.000549 0.000493 0.000329 0.000165 0.000601 0.000389 0.000386 0.000070 0.000573
Lipid Particles 0.001343 0.001225 0.000105 0.000465 0.002757 0.001740 0.000426 0.000489 0.001920 0.001551 0.000207 0.000476
Mitochondria 0.001639 0.001229 0.000986 0.003188 0.001750 0.002441 0.001490 0.002776 0.001684 0.001996 0.001147 0.002996
Mitotic Spindle 0.001778 0.002060 0.000545 0.016528 0.001702 0.003550 0.000759 0.018820 0.001747 0.003002 0.000613 0.017592
None 0.002461 0.004145 0.003053 0.005886 0.006442 0.006289 0.002815 0.011010 0.004087 0.005501 0.002977 0.008264
Nuclear Periphery 0.001054 0.001636 0.001377 0.003263 0.001060 0.001604 0.001968 0.003028 0.001056 0.001616 0.001565 0.003154
Nuclear Periphery Foci 0.000502 0.001542 0.000188 0.000936 0.001061 0.000587 0.000622 0.001222 0.000730 0.000938 0.000326 0.001069
Nucleolus 0.001028 0.001324 0.000190 0.000732 0.001649 0.001492 0.000447 0.001069 0.001281 0.001430 0.000272 0.000888
Nucleus 0.840608* 0.799917* 0.919253* 0.815086* 0.799167* 0.805252* 0.889951* 0.811831* 0.823688* 0.803291* 0.909915* 0.813576*
Peroxisomes 0.000596 0.000653 0.000016 0.000121 0.001203 0.001220 0.000072 0.000145 0.000844 0.001011 0.000034 0.000132
Vacuole 0.005044 0.011542 0.004787 0.021414 0.008216 0.008683 0.017056 0.021268 0.006339 0.009734 0.008697 0.021346
Vacuole Periphery 0.000268 0.000233 0.000048 0.001210 0.000167 0.000636 0.000264 0.000636 0.000227 0.000488 0.000117 0.000944

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.68 -3.25 -11.90 -5.11 -15.05 0.30 -5.01 -4.31 -8.22 1.88 -1.52 -5.06 -9.93 -9.68 -10.71
Bud Neck -4.68 -13.63 -24.07 -21.54 -15.74 -7.37 -16.61 -24.10 -14.63 -4.64 -7.95 -21.33 -33.37 -24.06 -16.29
Bud Site -5.64 -5.66 -12.12 -8.43 -9.83 -4.23 -9.64 -10.83 -5.43 -1.31 -6.46 -10.47 -16.16 -9.47 -9.88
Cell Periphery -0.54 1.41 -2.14 -3.65 -7.13 1.08 -6.08 -1.33 -3.56 6.04 0.39 -0.51 -2.21 -6.26 -2.67
Cytoplasm -2.36 21.29 18.89 17.06 -2.01 1.69 22.65 17.42 18.46 -8.24 -0.92 30.32 25.60 25.29 -5.83
Cytoplasmic Foci 0.90 12.58 10.98 7.92 -3.12 6.37 11.85 11.66 8.08 -0.59 5.47 17.31 16.01 11.25 -3.06
Eisosomes -2.52 5.87 -12.38 -9.58 -18.70 7.52 -8.89 -3.60 -15.98 7.04 4.64 -2.21 -12.04 -18.52 -10.30
Endoplasmic Reticulum -12.12 -7.14 -18.24 -0.14 -14.36 -0.58 -20.75 -13.29 -12.02 11.43 -9.86 -19.53 -22.54 -9.54 -3.25
Endosome -4.20 4.12 -3.04 1.28 -6.08 -1.24 -0.23 -0.54 0.59 -0.24 -3.58 2.51 -2.44 1.25 -4.28
Golgi -0.67 2.03 0.36 1.10 -2.59 0.95 2.02 1.25 0.45 -0.97 0.12 2.99 1.05 1.08 -2.78
Lipid Particles 0.62 6.60 3.07 3.43 -10.57 1.51 3.59 3.41 2.84 -1.80 1.14 6.09 4.40 3.44 -11.24
Mitochondria 0.86 1.17 -2.96 -4.83 -8.28 -1.03 0.07 -2.07 -0.90 -7.58 -0.70 1.07 -3.62 -2.87 -11.71
Mitotic Spindle -0.22 1.45 -5.86 -5.58 -6.60 -1.97 0.07 -6.35 -5.24 -6.57 -1.89 1.25 -8.62 -7.38 -9.43
None -2.28 -1.10 -7.11 -2.13 -7.89 0.20 4.29 -1.32 -1.67 -6.11 -2.27 3.34 -4.72 -2.00 -9.15
Nuclear Periphery -6.26 -8.73 -17.50 -12.10 -13.27 -3.07 -10.49 -17.52 -9.17 -8.89 -4.99 -12.93 -24.73 -13.65 -16.83
Nuclear Periphery Foci -4.33 3.14 -1.79 2.60 -3.65 2.09 1.64 1.27 -1.45 -0.66 -0.62 3.08 -0.09 0.60 -3.85
Nucleolus -0.53 3.02 0.01 0.60 -5.12 0.28 3.60 0.97 0.74 -3.48 -0.51 4.72 0.58 1.12 -6.23
Nucleus 5.67 -15.65 5.45 -0.86 20.96 -0.86 -10.17 -2.21 -1.51 9.35 3.83 -19.13 2.57 -1.49 23.37
Peroxisomes -0.16 3.83 3.32 3.86 -5.46 -0.02 3.54 3.49 5.11 -0.82 -0.72 5.13 4.76 6.14 -5.22
Vacuole -6.95 -3.04 -16.11 -12.16 -15.03 0.20 -7.89 -12.17 -12.48 -4.61 -4.41 -7.35 -20.35 -17.66 -15.78
Vacuole Periphery 0.28 1.75 -0.78 -0.96 -1.60 -1.98 -0.47 -1.32 1.46 -0.42 -1.54 1.49 -1.00 0.38 -1.84
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages nucleus (52%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YGR126W

YGR126W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygr126w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available