Standard name
Human Ortholog
Description Putative component of the protein phosphatase type 2A complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.23 0.47 0.56 0.93 0.39 0.76 0.59 0.56 0.64 0.66 0.68 0.57 – – – 0.33 0.62 0.59 0.21 0.4 0.55 0.38 0.39 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 0 0.06 0.07 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0.13 0.13 0.17 0 0 0.05 0 0 0 0.05 0 0 – – – 0.33 0.22 0.24 0.65 0.42 0.3 0.28 0.29 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.68 0.58 0.44 0.11 0.62 0.26 0.41 0.42 0.26 0.27 0.25 0.32 – – – 0.47 0.42 0.29 0 0.08 0 0.07 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 0 2 1 2 4 8 – – – 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 0 1 3 1 1 5 – – – 0 0 0 1 13 5 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 2 1 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 1 2 4 5 7 12 8 16 – – – 1 0 0 0 0 0 0 0 0
Cytoplasm 23 205 101 280 138 282 340 285 378 390 315 315 – – – 24 53 10 25 186 109 5 20 11
Endoplasmic Reticulum 1 0 0 1 1 3 5 6 9 15 9 7 – – – 6 4 0 0 1 0 0 0 0
Endosome 0 2 3 0 2 1 4 1 2 1 0 3 – – – 0 0 0 3 7 2 0 0 0
Golgi 1 0 0 0 12 3 4 10 1 6 7 7 – – – 0 0 1 2 2 1 0 0 0
Mitochondria 3 7 0 1 1 6 9 3 47 18 26 37 – – – 1 0 0 1 4 3 0 0 0
Nucleus 13 58 30 3 14 18 19 20 22 31 18 19 – – – 24 19 4 80 192 58 4 14 4
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 2 – – – 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 2 2 2 0 1 – – – 0 0 0 0 2 0 0 2 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 2 0 0 – – – 0 0 0 1 6 4 0 1 1
Vac/Vac Membrane 67 254 80 32 217 96 236 213 155 159 117 176 – – – 34 36 5 4 35 7 0 3 1
Unique Cell Count 98 440 181 302 352 370 575 507 592 593 465 552 72 85 17 124 461 199 15 52 27
Labelled Cell Count 110 527 215 319 387 414 622 548 627 639 505 596 90 112 20 124 461 199 15 52 27


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 7.8 9.3 11.0 15.6 13.9 13.5 13.8 11.9 13.9 13.9 14.5 – – – 10.7 11.8 11.5 – 17.7 –
Std Deviation (1e-4) 1.8 1.3 1.5 2.0 2.4 2.5 2.7 3.1 3.0 3.6 4.0 4.1 – – – 2.2 2.0 2.6 – 5.4 –
Intensity Change (Log2) – – – 0.24 0.75 0.58 0.54 0.58 0.36 0.58 0.59 0.64 – – – 0.21 0.34 0.32 – 0.93 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 9.6 -3.6 4.9 0.8 0.1 2.0 2.4 2.8 0.3 – – – -3.2 1.0 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 3.9 0 3.2 3.6 – – – 0 0 0
Nucleus -6.6 -5.0 -4.6 -6.3 -5.6 -6.0 -5.0 -5.5 -6.1 – – – 2.9 1.1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole -8.5 3.8 -4.3 -0.8 -0.5 -4.6 -4.4 -4.7 -3.0 – – – 0.4 -0.3 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7494 6.3036 5.6764 4.8444 3.9567 5.451 8.3782 10.5443 9.5158 8.9639 8.6611 9.643 4.7518 6.5792 5.7578 5.7024 5.092 6.002
Actin 0.0329 0.0017 0.0143 0.0577 0.065 0.0066 0.0269 0.0003 0.0027 0.0019 0.0004 0.0024 0.0264 0.0063 0.0244 0.0054 0.0056 0.0116
Bud 0.0018 0.0017 0.0093 0.002 0.0007 0.0008 0.0005 0.0003 0.0005 0.0003 0.0005 0.0002 0.0008 0.0007 0.0034 0.0001 0.0002 0.0002
Bud Neck 0.0044 0.0004 0.0007 0.0024 0.0016 0.002 0.0043 0.0005 0.0009 0.0008 0.0018 0.0021 0.0024 0.0006 0.0023 0.001 0.0013 0.0022
Bud Periphery 0.002 0.0004 0.0026 0.001 0.0005 0.0003 0.0005 0.0001 0.0004 0.0001 0.0005 0.0001 0.0009 0.0004 0.0018 0 0.0001 0.0002
Bud Site 0.0033 0.0016 0.0049 0.0016 0.0008 0.0002 0.0044 0.001 0.0019 0.0002 0.0036 0.0001 0.0031 0.0049 0.0039 0.0004 0.0002 0.0003
Cell Periphery 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0 0.0002 0 0.0002 0.0001 0.0001 0 0 0
Cytoplasm 0.3977 0.5412 0.603 0.5906 0.5859 0.5257 0.5063 0.5974 0.6309 0.817 0.7955 0.6018 0.529 0.6569 0.6027 0.8177 0.7693 0.5175
Cytoplasmic Foci 0.0214 0.0038 0.0086 0.0397 0.0081 0.0109 0.014 0.0015 0.0043 0.0048 0.0028 0.0026 0.0253 0.0025 0.013 0.014 0.0094 0.0042
Eisosomes 0.0003 0 0.0001 0.0001 0.0011 0 0.0004 0 0 0 0 0 0.0003 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0104 0.0028 0.0024 0.0019 0.0071 0.0017 0.0074 0.0014 0.0025 0.0057 0.0034 0.0024 0.0071 0.0033 0.0023 0.0039 0.006 0.0031
Endosome 0.0422 0.001 0.0114 0.0049 0.0129 0.0062 0.0121 0.0006 0.0029 0.0019 0.0024 0.0031 0.0157 0.0019 0.0061 0.0045 0.0113 0.0089
Golgi 0.0135 0.0001 0.0024 0.0074 0.0111 0.0032 0.0044 0 0.0012 0.0002 0.0001 0.0003 0.0041 0.0003 0.0028 0.0014 0.0016 0.0033
Lipid Particles 0.0081 0.0001 0.0004 0.0117 0.0203 0.0065 0.0034 0 0.0028 0.0002 0 0.0002 0.0103 0.0002 0.0058 0.0025 0.0042 0.0005
Mitochondria 0.0103 0.0006 0.003 0.0023 0.0194 0.0049 0.0041 0.0002 0.0026 0.0002 0.0003 0.0005 0.0023 0.0037 0.0016 0.0002 0.0007 0.0024
None 0.0076 0.0046 0.0124 0.0021 0.0725 0.0026 0.0111 0.0032 0.0093 0.0068 0.0045 0.0116 0.0235 0.0226 0.0109 0.0259 0.0092 0.0049
Nuclear Periphery 0.034 0.0224 0.0105 0.0089 0.0266 0.0101 0.0189 0.0056 0.0074 0.0043 0.008 0.0095 0.0211 0.0103 0.0061 0.0054 0.0193 0.0131
Nucleolus 0.0073 0.0003 0.0008 0.0002 0.0019 0.0003 0.0005 0.0002 0.0003 0.0001 0.0001 0.0003 0.0027 0.0004 0.0005 0.0002 0.0014 0.0005
Nucleus 0.3824 0.4128 0.2988 0.2319 0.1381 0.4081 0.3688 0.385 0.3218 0.15 0.1714 0.3573 0.2881 0.2792 0.2959 0.0998 0.1424 0.422
Peroxisomes 0.0036 0.0002 0.0007 0.0253 0.0134 0.0018 0.0034 0.0001 0.0027 0.0002 0.0001 0.0001 0.0141 0.0003 0.0107 0.001 0.0009 0.0003
Punctate Nuclear 0.0092 0.003 0.011 0.0065 0.008 0.0064 0.0053 0.0019 0.004 0.0037 0.0029 0.0039 0.0186 0.0035 0.0038 0.0159 0.0143 0.0033
Vacuole 0.0051 0.0012 0.0019 0.0014 0.0031 0.0012 0.0021 0.0006 0.0007 0.0015 0.0014 0.0011 0.0035 0.0013 0.0016 0.0004 0.0016 0.001
Vacuole Periphery 0.0021 0.0003 0.0006 0.0004 0.0015 0.0004 0.0009 0.0001 0.0002 0.0002 0.0002 0.0003 0.0007 0.0006 0.0003 0.0001 0.0008 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.7058 8.718 21.2556 26.2222 21.6759 68.2284 41.2404 44.1952 44.1426 36.2196
Translational Efficiency 1.9048 5.0051 1.5654 1.4973 2.1428 2.3482 1.9846 2.0405 2.1234 2.1053

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
904 992 724 1513 2035 1933 2014 1520 2939 2925 2738 3033

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 989.77 889.06 1028.13 1112.11 835.56 995.30 1005.06 1062.00 882.99 959.27 1011.16 1087.00
Standard Deviation 185.75 150.98 162.14 432.51 111.71 129.88 155.73 208.39 155.95 146.32 157.78 340.16
Intensity Change Log 2 — -0.154813 0.054857 0.168134 — 0.252388 0.266466 0.345968 — 0.045917 0.155588 0.252268

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000751 0.000470 0.000167 0.002128 0.000089 0.000184 0.000274 0.000691 0.000293 0.000281 0.000246 0.001408
Bud Neck 0.008646 0.002631 0.007615 0.009280 0.001202 0.002861 0.005675 0.011620 0.003491 0.002783 0.006188 0.010453
Bud Site 0.007224 0.003448 0.001863 0.025885 0.001107 0.002669 0.001792 0.012873 0.002989 0.002933 0.001811 0.019364
Cell Periphery 0.000635 0.000171 0.000094 0.000299 0.000065 0.000062 0.000172 0.000213 0.000241 0.000099 0.000151 0.000255
Cytoplasm 0.166775* 0.516939* 0.193173* 0.237874* 0.599811* 0.537087* 0.120219* 0.251078* 0.466615* 0.530254* 0.139510* 0.244491*
Cytoplasmic Foci 0.006551 0.018263 0.000512 0.020983 0.021692 0.019304 0.001713 0.001810 0.017035 0.018951 0.001395 0.011374
Eisosomes 0.000027 0.000023 0.000008 0.000031 0.000010 0.000012 0.000020 0.000025 0.000016 0.000016 0.000017 0.000028
Endoplasmic Reticulum 0.009463 0.003448 0.002281 0.004649 0.001080 0.000779 0.003017 0.004489 0.003658 0.001685 0.002822 0.004568
Endosome 0.000940 0.003012 0.000266 0.023507 0.000805 0.001603 0.000420 0.002215 0.000847 0.002081 0.000379 0.012836
Golgi 0.000213 0.001231 0.000036 0.015594 0.000125 0.000642 0.000044 0.000402 0.000152 0.000842 0.000041 0.007980
Lipid Particles 0.000600 0.000996 0.000170 0.001581 0.000473 0.000692 0.000327 0.000810 0.000512 0.000795 0.000286 0.001195
Mitochondria 0.000924 0.000758 0.001502 0.014228 0.000209 0.000771 0.000836 0.003622 0.000429 0.000767 0.001012 0.008913
Mitotic Spindle 0.000826 0.001473 0.005978 0.139877* 0.000677 0.004554 0.003650 0.021117 0.000723 0.003509 0.004266 0.080360
None 0.009231 0.012229 0.006713 0.016170 0.008471 0.007183 0.009514 0.015531 0.008705 0.008894 0.008773 0.015850
Nuclear Periphery 0.000797 0.000461 0.002226 0.003028 0.000375 0.000539 0.001185 0.001918 0.000505 0.000513 0.001460 0.002472
Nuclear Periphery Foci 0.001828 0.003696 0.000206 0.003613 0.000820 0.000364 0.000273 0.000741 0.001130 0.001494 0.000256 0.002174
Nucleolus 0.001198 0.000902 0.000196 0.001269 0.000293 0.000515 0.000525 0.001153 0.000572 0.000646 0.000438 0.001211
Nucleus 0.774060* 0.399405* 0.767749* 0.409820* 0.352239* 0.402251* 0.843242* 0.630924* 0.481986* 0.401286* 0.823280* 0.520627*
Peroxisomes 0.000200 0.000516 0.000023 0.002432 0.000168 0.000860 0.000053 0.000556 0.000178 0.000743 0.000045 0.001492
Vacuole 0.008365 0.029508 0.009140 0.057843 0.010137 0.016888 0.006983 0.036093 0.009592 0.021168 0.007553 0.046943
Vacuole Periphery 0.000746 0.000420 0.000082 0.009909 0.000150 0.000179 0.000067 0.002121 0.000334 0.000261 0.000071 0.006006

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.71 9.61 -1.38 -2.57 -4.37 -1.11 -10.60 -7.41 -4.79 -3.66 0.03 -2.19 -5.61 -5.02 -5.05
Bud Neck 6.15 -0.09 -2.66 -9.37 -1.77 -5.32 -11.02 -12.71 -10.01 -4.16 1.52 -7.76 -14.36 -14.50 -4.95
Bud Site 3.37 5.34 -2.04 -5.37 -7.12 -3.12 -4.29 -6.98 -5.25 -5.70 -0.02 1.89 -7.86 -7.70 -8.98
Cell Periphery 9.33 11.16 8.78 -1.92 -8.40 0.82 -10.51 -11.81 -11.87 -1.58 8.58 5.96 2.94 -10.83 -5.45
Cytoplasm -26.56 -0.26 -0.06 30.39 0.26 7.79 70.01* 54.08 42.70 -10.96 -6.51 49.88 41.92 53.25 -10.03
Cytoplasmic Foci -7.41 6.41 1.86 9.43 -5.90 1.22 16.73 16.25 13.53 -1.16 -1.73 17.66 14.65 15.70 -5.12
Eisosomes 0.96 8.67 -6.28 -7.19 -20.49 -2.35 -11.11 -16.74 -14.27 -7.04 -1.25 -4.54 -19.70 -17.29 -14.73
Endoplasmic Reticulum 10.12 11.22 7.67 -4.29 -6.27 2.24 -16.16 -17.07 -18.31 -4.16 8.93 -1.31 -7.08 -18.34 -6.20
Endosome -3.54 7.82 -4.66 -1.37 -5.52 -2.96 3.44 -2.60 1.13 -4.17 -4.62 6.25 -5.20 -1.50 -6.17
Golgi -1.25 4.58 -3.53 -1.35 -3.80 -1.91 2.08 -1.67 1.60 -3.72 -2.11 4.02 -3.69 -1.22 -3.99
Lipid Particles -2.99 3.11 -4.07 -0.94 -9.34 -1.84 1.19 -3.95 -1.26 -5.20 -3.23 2.72 -6.40 -2.10 -9.77
Mitochondria 0.56 -3.33 -5.42 -5.04 -4.07 -3.65 -17.81 -6.11 -4.15 -3.58 -2.18 -12.04 -8.26 -6.66 -5.81
Mitotic Spindle -1.20 -3.74 -13.11 -12.89 -10.10 -3.14 -4.64 -9.32 -7.51 -7.00 -3.32 -5.97 -15.92 -14.44 -12.87
None -1.46 2.07 -3.26 -1.38 -8.18 1.54 0.15 -2.89 -5.18 -3.15 0.05 0.98 -4.70 -5.29 -6.26
Nuclear Periphery 3.22 -10.83 -19.77 -22.73 -8.20 -4.89 -20.88 -24.74 -23.36 -11.86 -1.23 -21.86 -31.08 -31.50 -16.00
Nuclear Periphery Foci -4.52 6.47 0.43 5.02 -8.45 5.56 3.97 -1.16 -7.07 -5.31 -2.90 7.34 -1.51 1.89 -9.21
Nucleolus 0.95 4.36 -0.37 -0.99 -2.87 -2.87 -6.99 -3.63 -2.40 -2.15 -1.16 0.63 -2.87 -2.39 -3.18
Nucleus 27.15 -0.38 15.50 -14.34 16.98 -5.98 -65.14* -35.15 -27.96 20.39 8.96 -47.00 -18.49 -29.33 29.68
Peroxisomes -2.73 5.91 -1.28 0.40 -2.16 -2.80 1.73 -0.61 2.48 -1.96 -3.41 2.91 -1.44 2.10 -2.68
Vacuole -9.32 -3.31 -22.17 -14.91 -19.36 -5.81 -2.56 -16.71 -13.35 -15.36 -10.56 -4.00 -27.57 -21.03 -25.25
Vacuole Periphery 0.78 1.63 -1.48 -2.62 -3.39 -0.80 2.61 -2.14 -2.03 -2.46 0.56 2.01 -2.85 -3.42 -4.14
Standard name
Human Ortholog
Description Putative component of the protein phosphatase type 2A complex
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rts3

Rts3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rts3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available