Standard name
Human Ortholog
Description Phosphatase with sequence similarity to Cdc25p; Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.97 0.95 0.93 0.86 – 0.74 0.76 0.73 0.7 0.73 0.97 0.98 0.98 0.95 0.97 0.96 0.94 0.97 0.89 0.9 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.16 – 0.12 0.23 0.19 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 – 0.17 0.1 0.08 0.11 0.08 0 0 0 0.07 0 0 0 0 0.05 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 4
Bud 0 0 2 2 4 2 – 5 5 2 4 12 1 0 1 0 0 0 3 0 0 1 4 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 – 4 5 7 4 7 0 5 5 6 6 7 0 0 0 0 0 0
Cytoplasm 313 111 98 195 350 310 – 487 254 339 187 306 394 570 772 211 239 217 312 114 99 123 473 550
Endoplasmic Reticulum 1 0 1 1 1 3 – 2 6 12 2 3 5 5 2 5 7 4 1 0 0 0 4 4
Endosome 0 0 0 0 0 0 – 1 0 0 0 1 0 0 1 2 0 1 1 0 0 0 3 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 2 6
Mitochondria 2 2 4 13 11 56 – 78 76 90 76 105 4 0 2 3 6 2 1 0 2 1 3 1
Nucleus 2 2 0 2 3 2 – 30 6 30 10 13 13 9 12 5 2 4 1 0 0 1 5 3
Nuclear Periphery 0 0 0 1 0 1 – 3 0 3 2 2 0 1 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 1 – 0 0 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 0 5 26 16 – 112 35 35 29 33 0 14 10 16 8 9 4 0 5 6 22 18
Unique Cell Count 321 113 101 206 377 361 662 336 463 269 417 408 583 791 223 246 225 331 118 111 137 527 610
Labelled Cell Count 325 116 106 220 395 391 723 387 520 316 483 418 604 807 248 269 244 331 118 111 137 527 610


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 9.1 6.2 5.8 6.4 5.8 – 6.2 5.8 6.2 6.1 6.4 9.3 9.4 9.1 9.3 9.2 9.3 8.0 8.7 8.7
Std Deviation (1e-4) 1.2 1.9 0.9 1.1 1.2 1.3 – 1.2 1.4 1.3 1.3 1.7 1.4 5.1 1.5 2.1 2.1 2.4 1.9 1.8 1.7
Intensity Change (Log2) – – – -0.07 0.05 -0.07 – 0.0 -0.08 0.02 -0.0 0.05 0.59 0.61 0.56 0.6 0.58 0.6 0.38 0.5 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.5 -3.1 – -5.2 -4.8 -5.2 -5.6 -5.1 -0.2 0.5 0.3 -1.0 0.1 -0.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0 3.1 – 2.4 4.3 3.8 5.1 4.7 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 2.6 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 – 4.5 3.4 2.9 3.4 2.9 0 0 0 0 0 0