Standard name | |
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Human Ortholog | |
Description | Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 10 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 5 | 6 | 10 | 18 | 4 | – | 11 | 20 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 135 | 78 | 165 | 292 | 363 | 351 | 497 | 598 | 247 | – | 255 | 317 | 184 | 413 | 427 | 513 | 284 | 342 | 121 | 84 | 159 | 156 | 241 | 265 |
Endoplasmic Reticulum | 0 | 2 | 1 | 1 | 1 | 4 | 29 | 29 | 13 | – | 19 | 26 | 2 | 0 | 6 | 16 | 17 | 15 | 0 | 1 | 2 | 0 | 0 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 14 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 2 | 11 | 1 | 2 | 2 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 3 | 4 |
Unique Cell Count | 135 | 80 | 166 | 293 | 364 | 357 | 525 | 632 | 263 | 288 | 371 | 186 | 418 | 435 | 516 | 287 | 342 | 125 | 90 | 166 | 163 | 276 | 295 | |
Labelled Cell Count | 135 | 80 | 169 | 295 | 371 | 362 | 536 | 648 | 268 | 304 | 390 | 188 | 419 | 436 | 534 | 305 | 359 | 125 | 90 | 166 | 163 | 276 | 295 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 162.2 | 133.4 | 88.2 | 86.2 | 90.1 | 77.1 | 72.8 | 66.7 | 57.5 | – | 49.7 | 46.7 | 155.1 | 153.6 | 129.9 | 133.2 | 162.4 | 160.0 | 123.2 | 109.9 | 124.3 |
Std Deviation (1e-4) | 35.1 | 35.4 | 15.4 | 18.0 | 19.4 | 17.6 | 16.5 | 16.5 | 17.1 | – | 14.3 | 13.5 | 40.7 | 38.7 | 33.9 | 40.7 | 44.9 | 49.0 | 28.4 | 36.2 | 35.5 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.03 | -0.19 | -0.28 | -0.4 | -0.62 | – | -0.83 | -0.92 | 0.81 | 0.8 | 0.56 | 0.6 | 0.88 | 0.86 | 0.48 | 0.32 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.4 | 0.6 | -1.0 | -2.6 | -2.7 | -2.8 | – | -4.2 | -4.9 | -0.5 | -0.6 | -1.1 | 0 | -0.5 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.7 | 2.4 | 2.5 | – | 3.0 | 3.1 | 0 | 0 | 0 | 0 | 2.8 | 2.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 138.6889 | 189.5873 | 160.3022 | 163.1751 | 140.9596 | 161.1963 | 160.5591 | 217.8931 | 207.3603 | 194.4177 | 175.998 | 196.2954 | 142.7447 | 180.1413 | 160.3822 | 153.3346 | 152.1619 | 161.5852 |
Actin | 0.0022 | 0.0001 | 0.003 | 0.0001 | 0.0034 | 0.0239 | 0.0031 | 0.0002 | 0 | 0.0008 | 0.0027 | 0.0002 | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud | 0.001 | 0.0001 | 0.001 | 0.0002 | 0.0011 | 0.0002 | 0.0014 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0033 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0005 | 0 | 0.0003 | 0 | 0.0017 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0009 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8051 | 0.9622 | 0.8772 | 0.915 | 0.8621 | 0.9111 | 0.8847 | 0.9857 | 0.9792 | 0.9237 | 0.8675 | 0.9258 | 0.9336 | 0.9906 | 0.9663 | 0.9702 | 0.9828 | 0.9942 |
Cytoplasmic Foci | 0.0091 | 0 | 0.0131 | 0.0001 | 0.0038 | 0.0059 | 0.0038 | 0.0001 | 0.0005 | 0.0224 | 0.0029 | 0.0028 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0.0006 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0012 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0023 | 0.0001 | 0.0001 | 0.0008 | 0.0042 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endosome | 0.0023 | 0 | 0.0015 | 0.0001 | 0.002 | 0.002 | 0.0077 | 0 | 0 | 0.0045 | 0.0415 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 |
Golgi | 0.0004 | 0 | 0.0009 | 0 | 0.0023 | 0.0029 | 0.0008 | 0.0001 | 0 | 0.0015 | 0.0073 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0016 | 0 | 0.0035 | 0 | 0.0152 | 0.0088 | 0.0012 | 0.0001 | 0 | 0.0022 | 0.0024 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0103 | 0.0004 | 0.0106 | 0.0003 | 0.0135 | 0.0008 | 0.0048 | 0.002 | 0.0001 | 0.0018 | 0.0068 | 0.0022 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 |
None | 0.0081 | 0.0001 | 0.0083 | 0.0002 | 0.0097 | 0.0015 | 0.0176 | 0.0003 | 0.0018 | 0.0001 | 0.0007 | 0.0071 | 0.003 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.0178 | 0.0067 | 0.006 | 0.006 | 0.0071 | 0.0033 | 0.0183 | 0.0008 | 0.0011 | 0.026 | 0.0316 | 0.0044 | 0.0065 | 0.0021 | 0.0024 | 0.0025 | 0.0029 | 0.0006 |
Nucleolus | 0.0239 | 0.0001 | 0.0136 | 0.0001 | 0.0051 | 0.0001 | 0.006 | 0.0004 | 0 | 0.0001 | 0.001 | 0.0119 | 0.0028 | 0 | 0.001 | 0 | 0 | 0 |
Nucleus | 0.1 | 0.0286 | 0.0509 | 0.0742 | 0.0405 | 0.0114 | 0.0376 | 0.0086 | 0.0166 | 0.0102 | 0.017 | 0.0366 | 0.0456 | 0.0065 | 0.0282 | 0.0263 | 0.0123 | 0.0046 |
Peroxisomes | 0.0006 | 0 | 0.0008 | 0 | 0.0166 | 0.024 | 0.0006 | 0 | 0 | 0.0037 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0017 | 0 | 0.0007 | 0.0001 | 0.0006 | 0.0014 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0026 | 0.0006 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 |
Vacuole | 0.0077 | 0.0007 | 0.0029 | 0.002 | 0.0059 | 0.0008 | 0.0041 | 0.0002 | 0.0002 | 0.0002 | 0.0055 | 0.0029 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 |
Vacuole Periphery | 0.0045 | 0.0005 | 0.003 | 0.0012 | 0.0041 | 0.0005 | 0.003 | 0.0003 | 0.0001 | 0.0012 | 0.0053 | 0.0039 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 269.3456 | 221.1621 | 176.2206 | 206.9332 | 206.9629 | 287.6347 | 249.7698 | 233.3789 | 256.6299 | 243.0745 |
Translational Efficiency | 1.5094 | 1.3358 | 1.3465 | 1.304 | 1.341 | 1.4763 | 1.1755 | 1.2675 | 1.184 | 1.2016 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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